BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,494,066	G→C	7.5%	intergenic (+114/‑128)	RCAP_rcc02319 → / → RCAP_rcc02320	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,494,066	0	A→C	7.5%	21.4 ‑1.1 ‑1.0	141	intergenic (+114/‑128)	RCAP_rcc02319/RCAP_rcc02320	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (7/3): ref base (63/60): total (75/66)
Fisher's exact test strand distribution p-value = 3.32e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.04e-01

Only 100 of 150 total aligned reads displayed.


CCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGA.AGGGGGGCA.AGCG.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493965‑2494152


cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.T.CAg                                                                                            <  1:2790042/101‑1 (MQ=255)


            aaGCGGGCTCCCGCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCa                                                                                 <  1:2260694/101‑1 (MQ=18)


            aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCa                                                                                 <  1:1431276/101‑1 (MQ=18)


             aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCag                                                                                <  1:4753551/101‑2 (MQ=18)


              gCGGACTCCCCCCGGGAAATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCaga                                                                               >  1:4734439/1‑99 (MQ=18)


                ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAC.TGGC..GAA.G.CCAG.AGGGGGGCagcgc                                                                             >  1:4638215/1‑97 (MQ=17)


                ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGACCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCagcgc                                                                             >  1:2769520/1‑97 (MQ=17)


                 gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.c.c                                                                        >  1:2725543/1‑101 (MQ=17)


                  aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.c.cc                                                                       >  1:283443/1‑101 (MQ=18)


                  aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.c.cc                                                                       >  2:3025943/1‑101 (MQ=18)


                  aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.c.cc                                                                       >  2:2414556/1‑101 (MQ=18)


                   cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG..AGGGGGGC..AGCG.c.ccc                                                                      <  2:2954777/101‑1 (MQ=17)


                   cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.c.ccc                                                                      <  2:196547/101‑1 (MQ=17)


                   cTCCCCCCGGGATAGTTGCAGCAAGATGAATCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.c.ccc                                                                      <  2:43056/101‑1 (MQ=17)


                    tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.c.cccc                                                                     >  2:128706/1‑101 (MQ=17)


                    tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.c.cccc                                                                     >  1:1565968/1‑101 (MQ=18)


                      cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.c.ccccct                                                                   <  1:4471390/101‑3 (MQ=17)


                      cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.c.ccccct                                                                   <  2:848858/101‑3 (MQ=17)


                         ccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.c.ccccctgcc                                                                <  2:4018385/101‑6 (MQ=17)


                          cGGAATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGccc                                                                <  1:2203745/101‑1 (MQ=17)


                            ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGC.AGCG.C.CCCCTGCCCcg                                                              <  1:4015443/101‑1 (MQ=0)


                            ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCcg                                                              <  1:4417932/101‑1 (MQ=17)


                             gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGC.AGCG.C.CCCCTGCCCcgc                                                             >  1:4404457/1‑100 (MQ=2)


                               tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGC.AGCG.C.CCCCTGCCCcgccg                                                           >  2:1139753/1‑101 (MQ=2)


                               tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCcggcg                                                           >  1:1636751/1‑101 (MQ=18)


                                atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.CACCCCTGCCCcggcgg                                                          >  2:2029738/1‑101 (MQ=18)


                                 tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCcggcggc                                                         >  2:977702/1‑100 (MQ=17)


                                   tGCAGCAATATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTcc                                                       >  1:3303403/1‑101 (MQ=17)


                                   tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTcc                                                       >  2:263673/1‑101 (MQ=17)


                                   tGCAGCAAGATGAAACCGGATGCCTTTCATCTGGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTcc                                                       <  1:3190927/101‑1 (MQ=17)


                                    gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCg                                                      >  1:4463377/1‑101 (MQ=17)


                                      agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTg                                                    <  1:2414691/101‑1 (MQ=17)


                                      agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTg                                                    <  1:998655/101‑1 (MQ=17)


                                        caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTggc                                                  <  1:2524071/101‑1 (MQ=17)


                                        caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGG.A.AGCGCC.CCCCTGCCCCGGCGGTCCGTggc                                                  >  1:345412/1‑101 (MQ=18)


                                         aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGCCCGTggcg                                                 >  1:34950/1‑101 (MQ=17)


                                          aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTggcgg                                                >  2:1161814/1‑101 (MQ=18)


                                            aTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGgcggcg                                              >  2:1882578/1‑101 (MQ=18)


                                              gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGcc                                            >  1:2626861/1‑101 (MQ=17)


                                              gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGG.A.AGCGCC.CCCCTGCCCCGGCGGTCCGGGGCGGGGcc                                            >  2:3683987/1‑101 (MQ=17)


                                                aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          <  1:4650818/101‑1 (MQ=17)


                                                 aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         >  1:2685816/1‑101 (MQ=18)


                                                 aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         >  1:812992/1‑101 (MQ=17)


                                                 aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                         <  1:3335693/101‑1 (MQ=17)


                                                  ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                        <  1:2623656/101‑1 (MQ=17)


                                                   ccgATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                       <  2:1774723/99‑1 (MQ=17)


                                                   cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                       <  2:4640867/101‑1 (MQ=17)


                                                   cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                       >  1:769470/1‑101 (MQ=18)


                                                    ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGCcg                                      >  1:1223598/1‑101 (MQ=17)


                                                    ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                      >  1:3101667/1‑101 (MQ=17)


                                                     gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                     <  1:2311185/101‑1 (MQ=17)


                                                      aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCAGAGC..C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGatcgg                                    >  2:857681/1‑97 (MQ=17)


                                                      aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                                    <  1:1172984/101‑1 (MQ=17)


                                                      aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                                    <  2:1430771/101‑1 (MQ=17)


                                                       tgctttacgtCTTGCCCGACATATTCCGGGGGGCAAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCCTGAcgggg                                   >  2:142639/10‑100 (MQ=9)


                                                       tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                   <  1:4037518/101‑1 (MQ=17)


                                                         ccTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                                 <  1:1254947/101‑1 (MQ=17)


                                                          cTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAG.AGGGGGGCA.GCAC.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGGCGTGAcgggcggg                                >  2:4502618/1‑101 (MQ=17)


                                                           tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTTCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                               >  1:2587620/1‑101 (MQ=17)


                                                           tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                               <  2:944117/101‑1 (MQ=17)


                                                           tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                               <  1:4605986/101‑1 (MQ=17)


                                                           tttCATCTTCCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                               <  2:4386003/101‑1 (MQ=17)


                                                            ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                              >  2:1718966/1‑101 (MQ=18)


                                                             tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggggg                             >  2:3581/1‑100 (MQ=18)


                                                             tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                             <  2:2564012/101‑1 (MQ=18)


                                                             tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                             <  2:4272800/101‑1 (MQ=18)


                                                             tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGAGGCGGCGCCTTGACGGGCGGGGgc                             >  2:2012970/1‑101 (MQ=17)


                                                              cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcg                            >  1:3806231/1‑101 (MQ=18)


                                                               aTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCGCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggggggg                           >  1:4009401/1‑98 (MQ=17)


                                                                tCTTTCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                          <  2:2176871/101‑1 (MQ=18)


                                                                 cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                         >  2:1633663/1‑101 (MQ=18)


                                                                   tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGGGCCACGAAGGGCCGGGGCGCgggg                       >  1:835590/1‑100 (MQ=9)


                                                                   tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGc                       <  1:617765/101‑1 (MQ=18)


                                                                    gCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                      >  2:4636090/1‑101 (MQ=18)


                                                                      ccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                    <  2:2304365/101‑1 (MQ=18)


                                                                      ccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                    <  2:3593736/101‑1 (MQ=18)


                                                                      ccGAAATACCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                    <  1:2122/101‑1 (MQ=18)


                                                                        gAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                  <  1:98413/101‑1 (MQ=18)


                                                                        gAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                  <  1:56142/101‑1 (MQ=18)


                                                                        gAAATATCCCGGGGGGCGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGGCCCTTCACGGGCGGGGGGGCGGGCATTaa                  >  2:1959993/1‑101 (MQ=12)


                                                                         aaaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                 <  2:4734183/101‑1 (MQ=18)


                                                                         aaaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                 <  1:459589/101‑1 (MQ=18)


                                                                          aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                >  1:1127494/1‑101 (MQ=21)


                                                                           atatCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg               <  2:4398025/101‑1 (MQ=21)


                                                                            taccccGGGGGGTGAA.TGGC..G.A.A.GCCA.GAGGGGGCC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa              <  2:1329142/98‑1 (MQ=21)


                                                                             atCCGGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa             <  2:81842/101‑1 (MQ=21)


                                                                             atCCCGGGGGGGGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCGGGCATTAACCGaa             >  2:2821257/1‑101 (MQ=21)


                                                                              tCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa            <  1:4150127/101‑1 (MQ=21)


                                                                                  ggggggTGAA.TGGC..GAA.G.CCAG.AGGGCGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc        >  1:4254827/1‑101 (MQ=25)


                                                                                   gggggTGAA.TGGC..GAA.GCCAG..AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       >  1:316332/1‑101 (MQ=25)


                                                                                   gggggTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       <  1:4292049/101‑1 (MQ=25)


                                                                                   gggggTGAA.TGGC..G.AGG.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       <  2:417052/101‑1 (MQ=25)


                                                                                     gggTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc     <  1:749122/101‑1 (MQ=25)


                                                                                     gggTGAA.TGGC..G.A.A.GAGAGAGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc     <  2:71212/101‑1 (MQ=37)


                                                                                      ggTGAA.TGGC..GAA.GCCAG..AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCGGGCATTAACCGAAATTGCaaaa    >  1:16817/1‑99 (MQ=21)


                                                                                      ggTGAA.TGGC..GAA.GCCAG..AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa    >  2:377256/1‑101 (MQ=25)


                                                                                      ggTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa    <  1:2920432/101‑1 (MQ=25)


                                                                                       gTGAA.TGGC..GAA.GCCAG..AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg   >  1:3642685/1‑101 (MQ=25)


                                                                                       gTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg   <  1:3047534/101‑1 (MQ=25)


                                                                                        tGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGa  <  1:2132218/101‑1 (MQ=25)


CCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGA.AGGGGGGCA.AGCG.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493965‑2494152

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 24 ≤


ATCG

< 32 ≤


ATCG

< 40 ≤


ATCG