Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,523,952 T→G 100% I573S (ATC→AGC)  ppiD → peptidyl‑prolyl cis‑trans isomerase D

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,523,9520T→G100.0% 800.6 210I573S (ATC→AGC) ppiDpeptidyl‑prolyl cis‑trans isomerase D
Reads supporting (aligned to +/- strand):  new base (98/111):  ref base (0/0):  total (98/112)

Only 100 of 215 total aligned reads displayed.

CGACAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGATCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCAA  >  NC_014034/2523854‑2524049
                                                                                                  |                                                                                                 
cGACAAGGTCTTTGTCGTCCCCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGcc                                                                                                 <  1:1084290/101‑1 (MQ=255)
 gACAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCa                                                                                                <  2:4152028/101‑1 (MQ=255)
 gACAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCa                                                                                                <  1:4616182/101‑1 (MQ=255)
  aCAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAg                                                                                               <  2:268881/101‑1 (MQ=255)
  aCAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAg                                                                                               <  1:2157264/101‑1 (MQ=255)
  aCAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCAGTTCCGGCCGATCCCGAGCTGGCGAAGCTGCGCAAGACCTTTGACACCCGCCTGACCCAg                                                                                               >  1:3919345/1‑101 (MQ=255)
    aaGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGAt                                                                                             <  1:1151060/101‑1 (MQ=255)
     aGGTCTTTGTCGTCCCCGTGCGCGCGGGAAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATg                                                                                            <  2:137928/101‑1 (MQ=255)
     aGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATg                                                                                            >  1:765453/1‑101 (MQ=255)
      ggTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGc                                                                                           >  2:3748228/1‑101 (MQ=255)
       gTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCt                                                                                          >  1:4245859/1‑101 (MQ=255)
        tCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTc                                                                                         >  2:2437757/1‑101 (MQ=255)
        tCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTc                                                                                         <  2:3044553/101‑1 (MQ=255)
         cTTTGTCGTCACGGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCg                                                                                        <  2:196856/101‑1 (MQ=255)
          tttGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCgg                                                                                       >  1:961189/1‑101 (MQ=255)
            tGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGgcg                                                                                     >  1:4603919/1‑101 (MQ=255)
            tGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGgcg                                                                                     >  2:3991902/1‑101 (MQ=255)
            tGTCGTCACCGTGCGCGCGGTGAAACC.GCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGgcgc                                                                                    >  1:2767468/1‑101 (MQ=255)
             gtcgtcACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGgcgc                                                                                    >  1:1844145/1‑101 (MQ=255)
             gtcgtcACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGgcgc                                                                                    >  2:3611448/1‑101 (MQ=255)
               cggcACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCg                                                                                  <  2:2995208/98‑1 (MQ=255)
                  cACCGTGCGGGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATc                                                                               <  2:2295963/101‑1 (MQ=255)
                   aCCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCt                                                                              >  2:588156/1‑101 (MQ=255)
                    ccGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTg                                                                             <  2:2114605/101‑1 (MQ=255)
                     cGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTgg                                                                            >  1:2838745/1‑101 (MQ=255)
                       tGCGCGCGGTGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTggcg                                                                          <  1:3446487/101‑1 (MQ=255)
                          gcgcgGGGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGcc                                                                       <  1:2253364/101‑1 (MQ=255)
                           cgcgGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGAGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCt                                                                      >  2:848898/1‑101 (MQ=255)
                            gcgGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCGGATCTAGCGGCCtt                                                                     >  1:4099826/1‑101 (MQ=255)
                             cgGTGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTc                                                                    <  1:593466/101‑1 (MQ=255)
                              tgagAAACCGTCCGAGCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCt                                                                   <  2:755780/98‑1 (MQ=255)
                              ggggAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCt                                                                   <  2:4351518/98‑1 (MQ=255)
                              ggTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCt                                                                   <  1:3129133/101‑1 (MQ=255)
                               ttaaatcctgccgatccggccgatccCGAGCTGGTGCAGCCGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGGGCCGATCTGGGGGCCTTCTa                                                                  <  2:160133/92‑1 (MQ=255)
                                 gAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACg                                                                <  1:563637/101‑1 (MQ=255)
                                 gAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACg                                                                <  1:3812825/101‑1 (MQ=255)
                                 gAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACg                                                                >  1:395276/1‑101 (MQ=255)
                                      cggccgatccggccgatcCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGt                                                           >  1:2728657/1‑101 (MQ=255)
                                       ggcggatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTg                                                          <  1:163919/101‑1 (MQ=255)
                                       ggccgatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTg                                                          >  1:744662/1‑101 (MQ=255)
                                           gatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCcgc                                                      >  1:919134/1‑101 (MQ=255)
                                           gatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCcgc                                                      <  2:2900950/101‑1 (MQ=255)
                                           gatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCcgc                                                      >  1:4175267/1‑101 (MQ=255)
                                           gatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCcgc                                                      <  1:4515731/101‑1 (MQ=255)
                                            atccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCcgcg                                                     >  2:629290/1‑101 (MQ=255)
                                            atccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCcgcg                                                     >  1:4692606/1‑101 (MQ=255)
                                              ccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCa                                                   >  2:2472352/1‑101 (MQ=255)
                                              ccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTAACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCa                                                   >  2:3501164/1‑101 (MQ=255)
                                               cggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCag                                                  <  1:2934590/101‑1 (MQ=255)
                                               cggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCag                                                  <  1:1141005/101‑1 (MQ=255)
                                                    gatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccga                                             <  1:2819816/101‑1 (MQ=255)
                                                     atccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgag                                            <  1:1820356/101‑1 (MQ=255)
                                                      tccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgagg                                           >  1:2525323/1‑101 (MQ=255)
                                                      tccCGAGCGGGTGCGGTTGCGCAAGACTTTTGGCGCCCGGCTGAGCCAGATGCTCGGCGCGCACCTGGGGGCCTTCTACGCCCGTGCCCCCCaggccgatg                                           <  2:3112808/101‑3 (MQ=255)
                                                       cccGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgaggc                                          >  2:2825294/1‑101 (MQ=255)
                                                       cccGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgaggc                                          <  1:3116677/101‑1 (MQ=255)
                                                          gAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCgg                                       >  1:799195/1‑101 (MQ=255)
                                                           aGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCggg                                      <  2:2525357/101‑1 (MQ=255)
                                                           aGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCAGAGGCCggg                                      >  2:444617/1‑101 (MQ=255)
                                                            gctagcgctgCTGTGAAAGCCCCTTGACACCCGGATGATCCAGATGCTCGTCGCCGATCTGGCGTCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGa                                     <  2:1807414/92‑1 (MQ=255)
                                                             cTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGAt                                    >  2:2048581/1‑101 (MQ=255)
                                                             cTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGAt                                    <  1:170222/101‑1 (MQ=255)
                                                             cTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGAt                                    <  1:642864/101‑1 (MQ=255)
                                                             cTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCGTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGAt                                    >  1:4076109/1‑101 (MQ=255)
                                                              tGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATc                                   >  1:2672535/1‑101 (MQ=255)
                                                               ggTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCa                                  <  1:2409051/101‑1 (MQ=255)
                                                               ggTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCa                                  >  1:2542247/1‑101 (MQ=255)
                                                                  gCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCAccc                               <  2:3883177/101‑1 (MQ=255)
                                                                    aGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTc                             >  2:1055141/1‑101 (MQ=255)
                                                                    aGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTc                             >  1:3772056/1‑101 (MQ=255)
                                                                     gCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCg                            <  1:3344987/101‑1 (MQ=255)
                                                                      cTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGa                           <  2:799122/101‑1 (MQ=255)
                                                                      cTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGa                           >  2:2013055/1‑101 (MQ=255)
                                                                       tGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGAc                          <  2:2730209/101‑1 (MQ=255)
                                                                       tGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGAc                          <  2:395232/101‑1 (MQ=255)
                                                                       gaggcAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGAc                          <  1:4683603/98‑1 (MQ=255)
                                                                        gcgcAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACa                         <  1:2179939/101‑1 (MQ=255)
                                                                           cAAGCCCTTTGCCCCCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAgcg                      <  1:583061/101‑1 (MQ=255)
                                                                           cAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGTCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCCGGCCGAGGCCGGGATCACCCTCGACAgcg                      <  2:3915118/101‑1 (MQ=255)
                                                                           cAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAgcg                      >  2:2285205/1‑101 (MQ=255)
                                                                             aGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGcgcg                    <  2:3404339/101‑1 (MQ=255)
                                                                              gACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCga                   >  2:4302733/1‑101 (MQ=255)
                                                                                ccTTTGACGCCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgatg                 <  2:4549579/101‑1 (MQ=255)
                                                                                ccTTTGACCCCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgatg                 <  1:446914/101‑1 (MQ=255)
                                                                                ccTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgatg                 <  1:3257879/101‑1 (MQ=255)
                                                                                ccTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgatg                 <  1:1360236/101‑1 (MQ=255)
                                                                                ccTCTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgatg                 <  1:3711723/101‑1 (MQ=255)
                                                                                  tttGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgatgat               <  2:1260342/101‑1 (MQ=255)
                                                                                   ttGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATc              >  2:1436720/1‑101 (MQ=255)
                                                                                      acccccGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCt           <  1:2471924/98‑1 (MQ=255)
                                                                                        acCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGc         <  2:919057/101‑1 (MQ=255)
                                                                                         cccGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGcc        <  1:390817/101‑1 (MQ=255)
                                                                                           cGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGt      >  1:1510335/1‑101 (MQ=255)
                                                                                           cGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGt      <  1:4568697/101‑1 (MQ=255)
                                                                                            ggCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTg     >  1:3976020/1‑101 (MQ=255)
                                                                                              cTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCa   <  2:4671680/101‑1 (MQ=255)
                                                                                              cTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCa   >  2:2593436/1‑101 (MQ=255)
                                                                                               tGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCaa  >  2:4284022/1‑101 (MQ=255)
                                                                                               tGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCaa  <  1:3581049/101‑1 (MQ=255)
                                                                                               tGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCaa  >  1:1919694/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CGACAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGATCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCAA  >  NC_014034/2523854‑2524049

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG