Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,544,260 C→A 2.1% V190V (GTG→GTT)  RCAP_rcc02372 ← monosacharide ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,544,2600C→A 2.1% 2.3 ‑1.0 ‑0.2239V190V (GTG→GTT) RCAP_rcc02372monosacharide ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (122/112):  total (126/113)
Fisher's exact test strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.80e-01

Only 100 of 253 total aligned reads displayed.

CACCGCGATGCGGTCGGACACGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATCGCCACGGTCTGGC  >  NC_014034/2544161‑2544360
                                                                                                   |                                                                                                    
caCCGCGATGCGGTCGGACACGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAaca                                                                                                     >  1:4477/1‑101 (MQ=255)
caCCGCGATGCGGTCGGACACGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAaca                                                                                                     >  1:1774653/1‑101 (MQ=255)
 aCCGCGAGGCGGTCGGACACGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAacac                                                                                                    <  2:3226954/101‑1 (MQ=255)
   cgcgATGCGGTCGGACACGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCt                                                                                                  >  1:2617594/1‑101 (MQ=255)
   cgcgATGCGGTCGGACACGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCt                                                                                                  >  2:2902742/1‑101 (MQ=255)
    gcgATGCGGTCGGACACGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTc                                                                                                 >  1:1860171/1‑101 (MQ=255)
    gcgATGCGGTCGGACACGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTc                                                                                                 <  1:4511114/101‑1 (MQ=255)
    gcgATGCGGTCGGACACGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTc                                                                                                 <  2:2549164/101‑1 (MQ=255)
      gATGCGGTCGGACACGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTcgg                                                                                               >  2:1222243/1‑101 (MQ=255)
        tGCGGTCGGACACGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTcggcg                                                                                             >  2:2259252/1‑101 (MQ=255)
            gTCGGACACGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGGCt                                                                                         >  1:681204/1‑101 (MQ=255)
             tCGGACACGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTg                                                                                        <  2:3525419/101‑1 (MQ=255)
              cGGACACGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTgg                                                                                       >  1:1038964/1‑101 (MQ=255)
               ggACACGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTggg                                                                                      >  1:3854862/1‑101 (MQ=255)
               ggACACGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTggg                                                                                      >  2:4741268/1‑101 (MQ=255)
               ggACACGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAAAACCTCGGCGGTCTggg                                                                                      >  1:395245/1‑101 (MQ=255)
                gACACGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGc                                                                                     <  1:3742769/101‑1 (MQ=255)
                    cGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGAcc                                                                                 <  1:2475335/101‑1 (MQ=255)
                       cgcgGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGAccccc                                                                              >  2:4664445/1‑101 (MQ=255)
                        gcgGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCca                                                                             <  1:493037/101‑1 (MQ=255)
                        gcgGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCca                                                                             <  1:3210869/101‑1 (MQ=255)
                         cgGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCcag                                                                            >  2:3404569/1‑101 (MQ=255)
                         cgGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCcag                                                                            >  1:3457990/1‑101 (MQ=255)
                          ggACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCATCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCcagc                                                                           <  2:4101749/101‑1 (MQ=255)
                          ggACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCcagc                                                                           >  1:216081/1‑101 (MQ=255)
                          ggACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCcagc                                                                           <  1:3008950/101‑1 (MQ=255)
                           gACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCcagcg                                                                          >  2:537186/1‑101 (MQ=255)
                            aCATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCcagcgc                                                                         >  2:194107/1‑101 (MQ=255)
                              aTTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCcagcgcag                                                                       >  2:3381666/1‑101 (MQ=255)
                               ttGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCcagcgcagc                                                                      <  2:4391627/101‑1 (MQ=255)
                                tGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCcagcgcagcg                                                                     >  1:3143797/1‑101 (MQ=255)
                                tGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCcagcgcagcg                                                                     >  1:2119427/1‑101 (MQ=255)
                                tGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCcagcgcagcg                                                                     >  2:388762/1‑101 (MQ=255)
                                   ccATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTg                                                                  >  1:276193/1‑101 (MQ=255)
                                   ccATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTg                                                                  >  2:869763/1‑101 (MQ=255)
                                   ccATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTg                                                                  >  1:2324263/1‑101 (MQ=255)
                                    cATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTgg                                                                 >  2:138112/1‑101 (MQ=255)
                                     aTGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTggg                                                                >  2:2001416/1‑101 (MQ=255)
                                       gttgtGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCt                                                              <  1:1994200/101‑1 (MQ=255)
                                         tgtgGGAAATCAGGATCNCGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCa                                                            <  2:170970/101‑1 (MQ=255)
                                         tgtgGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCa                                                            <  1:190122/101‑1 (MQ=255)
                                           tgGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATc                                                          <  2:2148090/101‑1 (MQ=255)
                                           tgGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATc                                                          <  1:897235/101‑1 (MQ=255)
                                              gAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAg                                                       >  1:2935786/1‑101 (MQ=255)
                                                aaTCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCa                                                     >  1:2020403/1‑101 (MQ=255)
                                                 aTCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCAt                                                    <  1:2211957/101‑1 (MQ=255)
                                                  tCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATg                                                   <  2:2664601/101‑1 (MQ=255)
                                                   cAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGa                                                  <  1:2640410/101‑1 (MQ=255)
                                                   cAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGa                                                  >  1:1349088/1‑101 (MQ=255)
                                                   cAGGATCACGCCATAGCCGAGATCGCGCAGCCGCTAGATCAGGTTCAAAACCTCGGCGGTCTGGGCGACTCCCAGCGCAGCGGTGGGATCATCGAGCATGa                                                  >  2:624242/1‑101 (MQ=255)
                                                     ggATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCCTCGAGCATGACg                                                <  1:2576043/101‑1 (MQ=255)
                                                     ggATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACg                                                <  2:1902369/101‑1 (MQ=255)
                                                       aTCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGAt                                              <  2:2831430/101‑1 (MQ=255)
                                                         cACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCt                                            <  1:2097647/101‑1 (MQ=255)
                                                         cACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCt                                            <  2:317501/101‑1 (MQ=255)
                                                          aCGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCtt                                           <  1:3594706/101‑1 (MQ=255)
                                                            gCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCg                                         <  2:3268593/101‑1 (MQ=255)
                                                            gCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCg                                         <  2:1038885/101‑1 (MQ=255)
                                                               aTAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCt                                      >  2:951567/1‑101 (MQ=255)
                                                               aTAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCt                                      >  1:761746/1‑101 (MQ=255)
                                                                tAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTc                                     <  1:1686169/101‑1 (MQ=255)
                                                                tAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTc                                     <  2:2970501/101‑1 (MQ=255)
                                                                 aGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTcc                                    >  2:3467414/1‑101 (MQ=255)
                                                                 aGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTcc                                    >  2:2314081/1‑101 (MQ=255)
                                                                   ccGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCcag                                  <  2:3340858/101‑1 (MQ=255)
                                                                   ccGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCcag                                  >  2:244861/1‑101 (MQ=255)
                                                                    cgcgATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCcagc                                 <  2:2727842/101‑1 (MQ=255)
                                                                     gcgATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCcagca                                <  1:4115758/101‑1 (MQ=255)
                                                                      cgATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCcagcag                               <  2:1860059/101‑1 (MQ=255)
                                                                        aTCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGTTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCcagcagca                             <  1:2829238/101‑1 (MQ=255)
                                                                        aTCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCcagcagca                             <  2:555443/101‑1 (MQ=255)
                                                                         tCGCGCAGCCGCTGGATCAGGTTCAACACCTCGGCGGTCGGGGCGACCCCCAGCGCGGCGGTGGGATGAGCGAGCATGACGATCTTCGGCTCcagcagcag                            <  1:514347/101‑1 (MQ=255)
                                                                         tCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCcagcagcag                            <  2:761672/101‑1 (MQ=255)
                                                                          cgggccgCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCTCAGCGCTGGGCTCATCGAGCATGGCGATCTTCGGCTCCagcagcagc                           <  2:804109/95‑1 (MQ=255)
                                                                          cgcgCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCagcagcagc                           >  2:2619335/1‑101 (MQ=255)
                                                                          cgcgCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCagcagcagc                           <  2:2988209/101‑1 (MQ=255)
                                                                          cgcgCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCagcagcagc                           <  2:155995/101‑1 (MQ=255)
                                                                           gcgcAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCagcg                          <  2:183037/101‑1 (MQ=255)
                                                                            cgcAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCagcga                         <  1:73963/101‑1 (MQ=255)
                                                                                   gCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGcgcgcg                  >  2:1100460/1‑101 (MQ=255)
                                                                                   gCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGcgcgcg                  >  2:3561203/1‑101 (MQ=255)
                                                                                      cGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATc               >  1:1420681/1‑101 (MQ=255)
                                                                                      cGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATc               >  1:643031/1‑101 (MQ=255)
                                                                                       gATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATCg              >  2:3695730/1‑101 (MQ=255)
                                                                                        aTCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATCGc             <  2:3190421/101‑1 (MQ=255)
                                                                                        aTCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATCGc             >  1:1229481/1‑101 (MQ=255)
                                                                                         tCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATCGcc            >  1:3571878/1‑101 (MQ=255)
                                                                                          ctggTTCAACACCTCGGCGGGCTGGGTGTGCGCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATCGCCa           <  2:681140/99‑1 (MQ=255)
                                                                                          cAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATCGCCa           >  1:4022925/1‑101 (MQ=255)
                                                                                           aGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATCGCCAc          <  2:4357806/101‑1 (MQ=255)
                                                                                           aGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATCGCCAc          <  2:70475/101‑1 (MQ=255)
                                                                                               tCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATCGCCACGGTc      >  2:1026287/1‑101 (MQ=255)
                                                                                                cAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATCGCCACGGTCt     <  1:1654254/101‑1 (MQ=255)
                                                                                                cAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATCGCCACGGTCt     <  2:2107305/101‑1 (MQ=255)
                                                                                                cAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATCGCCACGGTCt     <  1:1100546/101‑1 (MQ=255)
                                                                                                cAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATCGCCACGGTCt     <  2:1613194/101‑1 (MQ=255)
                                                                                                 aaCACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATCGCCATGGCCTg    >  2:2069548/1‑101 (MQ=255)
                                                                                                  acacCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATCGCCACGGTCTgg   <  1:3381872/101‑1 (MQ=255)
                                                                                                   cacCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATCGCCACGGTCTGgc  <  1:981577/101‑1 (MQ=255)
                                                                                                   cacCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATCGCCACGGTCTGgc  <  2:186990/101‑1 (MQ=255)
                                                                                                   |                                                                                                    
CACCGCGATGCGGTCGGACACGGCGCGGACATTGTCCATGTTGTGGGAAATCAGGATCACGCCATAGCCGCGATCGCGCAGCCGCTCGATCAGGTTCAACACCTCGGCGGTCTGGGCGACCCCCAGCGCAGCGGTGGGCTCATCGAGCATGACGATCTTCGGCTCCAGCAGCAGCGAGCGCGCGATCGCCACGGTCTGGC  >  NC_014034/2544161‑2544360

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG