Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,549,153 G→A 100% G56S (GGC→AGC)  tonB → protein TonB

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,549,1530G→A100.0% 682.0 180G56S (GGC→AGC) tonBprotein TonB
Reads supporting (aligned to +/- strand):  new base (78/101):  ref base (0/0):  total (78/102)

Only 100 of 202 total aligned reads displayed.

GCTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACGGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCGA  >  NC_014034/2549056‑2549250
                                                                                                 |                                                                                                 
gCTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCACCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGGGGATCTGGACAGcc                                                                                                >  2:1203338/1‑101 (MQ=255)
  tGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGCGGATCTGGACAGCCTc                                                                                              >  2:4472245/1‑101 (MQ=255)
  tGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGGTCTGGACAGGCTc                                                                                              >  2:3406308/1‑101 (MQ=255)
  tGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGGTCAGGACCGCCTc                                                                                              >  2:1150767/1‑101 (MQ=255)
    gcgcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGa                                                                                            <  1:1527493/101‑1 (MQ=255)
      gcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAg                                                                                          <  2:2218290/101‑1 (MQ=255)
      gcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAg                                                                                          >  1:2137595/1‑101 (MQ=255)
       cTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGc                                                                                         >  2:2510388/1‑101 (MQ=255)
         gCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACCGCCTCGAAGCGa                                                                                       >  2:283987/1‑101 (MQ=255)
         gCATGCGGTCGCGGTTTCCGTACTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGa                                                                                       >  1:4587195/1‑101 (MQ=255)
          gaTGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGAt                                                                                      <  1:394760/100‑1 (MQ=255)
          cATGCGTTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGAt                                                                                      <  1:762155/101‑1 (MQ=255)
          cATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGAt                                                                                      >  2:3828010/1‑101 (MQ=255)
           aTGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATc                                                                                     <  1:3742825/101‑1 (MQ=255)
           aTGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATc                                                                                     <  1:3651634/101‑1 (MQ=255)
           aTGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATc                                                                                     >  2:1282877/1‑101 (MQ=255)
             gCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGGTCTGGACAGCCTCGAAGCGATcgc                                                                                   >  2:2426673/1‑101 (MQ=255)
             gCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACGGCCTCGAAGCGATcgc                                                                                   >  2:478019/1‑101 (MQ=255)
             gCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATcgc                                                                                   <  2:3807965/101‑1 (MQ=255)
              cGGGCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATcgcc                                                                                  >  1:2868879/1‑101 (MQ=255)
                gTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATcgccgc                                                                                >  1:3349681/1‑101 (MQ=255)
                  cgcgGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGCAGCGATCGCCGCGa                                                                              >  2:3374360/1‑101 (MQ=255)
                     ggTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCt                                                                           >  1:2788367/1‑101 (MQ=255)
                      gTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCtt                                                                          <  2:2014686/101‑1 (MQ=255)
                       tttCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtc                                                                         <  2:1528931/101‑1 (MQ=255)
                       tttCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtc                                                                         <  1:4014220/101‑1 (MQ=255)
                        ttCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGGGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcg                                                                        <  2:2315155/101‑1 (MQ=255)
                        ttCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcg                                                                        <  1:4309289/101‑1 (MQ=255)
                        ttCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGAGAGGATCTGGACAGACTCGAAGAGATAGCCGCGACCTtcg                                                                        >  1:2353375/1‑101 (MQ=255)
                         tCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgt                                                                       <  1:1912719/101‑1 (MQ=255)
                           cGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGGTGTGGGCAGCCTCGAAGTAATTGCCGCGACCCtcgtcg                                                                     >  1:2389139/1‑101 (MQ=255)
                           cGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACTGCCTCGAAGCGATCGCCGCGACCTtcgtcg                                                                     <  1:4227161/101‑1 (MQ=255)
                            gTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGa                                                                    >  1:1561319/1‑101 (MQ=255)
                             tGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGCCCCTCGTCGAt                                                                   >  1:2518351/1‑101 (MQ=255)
                             tGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGAt                                                                   >  2:66987/1‑101 (MQ=255)
                              gCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATc                                                                  <  1:672856/101‑1 (MQ=255)
                               ctctCGCTGACCGAGGCCGCCCCCGCGGCTCTGGTGGCGGCGGGCGCTGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGTCCTTCGTCGATCt                                                                 <  2:3588805/101‑1 (MQ=255)
                               ctctCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCt                                                                 >  1:3204214/1‑101 (MQ=255)
                                tctcGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGGGGGTCTGGACAGCCTCGGAGCGATCGCCGCGGCCTTCGACGATCTg                                                                >  1:3759612/1‑101 (MQ=255)
                                tctcGCACACCGCGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTg                                                                <  1:3585776/101‑1 (MQ=255)
                                 ctcGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTgg                                                               <  2:1692481/101‑1 (MQ=255)
                                 ctcGCAGACCGAGGCCGCCCCCGCGGAGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTgg                                                               >  2:3135117/1‑101 (MQ=255)
                                     cAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATTGCCGCGACCTTCGTCGATCTGGcgcc                                                           >  2:4717429/1‑101 (MQ=255)
                                     cAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgcc                                                           <  1:2013909/101‑1 (MQ=255)
                                     cAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgcc                                                           >  1:1224752/1‑101 (MQ=255)
                                      aGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgccg                                                          <  1:3601563/101‑1 (MQ=255)
                                       gACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGccccgc                                                         >  1:1018307/1‑101 (MQ=255)
                                       gACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGAAAGCCTCGGAGCGATCCCCGCCAACTTCGTCGATCTGGcgccgc                                                         >  2:4123425/1‑101 (MQ=255)
                                          cGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGa                                                      <  2:2156062/101‑1 (MQ=255)
                                          cGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGa                                                      >  1:3023681/1‑101 (MQ=255)
                                           gAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGAt                                                     <  2:18576/101‑1 (MQ=255)
                                           gAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGAt                                                     >  1:3282239/1‑101 (MQ=255)
                                           gAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGAt                                                     >  1:3675056/1‑101 (MQ=255)
                                             ggCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATcc                                                   <  1:1417946/101‑1 (MQ=255)
                                             ggCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATcc                                                   <  2:1971821/101‑1 (MQ=255)
                                              gccgccCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCa                                                  <  1:4642507/101‑1 (MQ=255)
                                              gccgccCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCa                                                  <  1:4704357/101‑1 (MQ=255)
                                               ccgccCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTTGATCTGGCGCCGCAGATCCAg                                                 <  1:3501443/101‑1 (MQ=255)
                                               ccgccCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAg                                                 <  2:463365/101‑1 (MQ=255)
                                                 gccCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCt                                               <  2:3499983/101‑1 (MQ=255)
                                                 gccCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCt                                               <  1:1772368/101‑1 (MQ=255)
                                                 gccCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCt                                               >  1:2400562/1‑101 (MQ=255)
                                                     ccGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgccc                                           <  2:4288942/101‑1 (MQ=255)
                                                     ccGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgccc                                           <  2:333425/101‑1 (MQ=255)
                                                      cgcgGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAG.CTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccga                                         <  2:3026679/101‑1 (MQ=255)
                                                         gtcgcTGGTGGCGGCGGGCGCGGGCGGCGAGTATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagc                                       <  1:1150853/99‑1 (MQ=255)
                                                            gcTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagcccg                                    <  1:4508026/101‑1 (MQ=255)
                                                            gcTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagcccg                                    <  2:2389005/101‑1 (MQ=255)
                                                            gcTGGGGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagcccg                                    <  1:516031/101‑1 (MQ=255)
                                                              tggtggCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGcccgagcccgag                                  <  1:1457386/101‑1 (MQ=255)
                                                              tggtggCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGcccgagcccgag                                  >  1:2220043/1‑101 (MQ=255)
                                                               ggtggCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGCGCCCggga                                 >  1:2159367/1‑101 (MQ=255)
                                                               ggtggCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCgaga                                 <  2:561835/101‑1 (MQ=255)
                                                               ggtggCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCgaga                                 >  1:1645928/1‑101 (MQ=255)
                                                                gtggCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGAt                                <  1:2435707/101‑1 (MQ=255)
                                                                 tggCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATg                               <  2:4500657/101‑1 (MQ=255)
                                                                  ggcggcGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGCTgg                              <  2:4023141/101‑1 (MQ=255)
                                                                  ggcggcGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATgg                              <  1:1282968/101‑1 (MQ=255)
                                                                      gggggcgcgggggGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCt                          <  1:478070/99‑1 (MQ=255)
                                                                       ggggcgcgggcgGCGAGGATCTGGACAGCCTCGAAGCGATCGCAGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTg                         <  1:284021/100‑1 (MQ=255)
                                                                        gggcgcgggcgGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGc                        >  2:2128485/1‑101 (MQ=255)
                                                                            gcgggcggCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCt                    >  2:1018125/1‑101 (MQ=255)
                                                                            gcgggcggCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCACTGCCGCt                    <  1:2925710/101‑1 (MQ=255)
                                                                             cgggcggCGAGGATCTGGACAGCGTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTg                   >  1:3819936/1‑101 (MQ=255)
                                                                                gcggcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGAcc                <  2:3697130/101‑1 (MQ=255)
                                                                                gcggcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGAcc                <  1:3982176/101‑1 (MQ=255)
                                                                                 cggcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACcg               >  1:858252/1‑101 (MQ=255)
                                                                                  ggcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACcgc              <  1:652218/101‑1 (MQ=255)
                                                                                  ggcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACcgc              <  2:2791276/101‑1 (MQ=255)
                                                                                    cgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCgcgc            >  2:4097219/1‑101 (MQ=255)
                                                                                    cgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCgcgc            >  1:264134/1‑101 (MQ=255)
                                                                                     gAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGcc           <  2:4257098/101‑1 (MQ=255)
                                                                                      aGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCg          >  2:1420127/1‑101 (MQ=255)
                                                                                       ggATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCgg         >  2:3148958/1‑101 (MQ=255)
                                                                                         aTCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTg       >  1:1244521/1‑101 (MQ=255)
                                                                                         aTCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTg       >  2:658894/1‑101 (MQ=255)
                                                                                           cTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGAt     >  2:3861628/1‑101 (MQ=255)
                                                                                             ggACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCg   <  2:1224662/101‑1 (MQ=255)
                                                                                             ggACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCg   >  1:2581167/1‑101 (MQ=255)
                                                                                              gACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCGa  <  2:3653182/101‑1 (MQ=255)
                                                                                                 |                                                                                                 
GCTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACGGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCGA  >  NC_014034/2549056‑2549250

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG