Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,554,356 T→C 100% N96D (AAC→GAC)  mraW ← S‑adenosyl‑methyltransferase MraW

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,554,3560T→C100.0% 874.7 230N96D (AAC→GAC) mraWS‑adenosyl‑methyltransferase MraW
Reads supporting (aligned to +/- strand):  new base (93/137):  ref base (0/0):  total (93/137)

Only 100 of 236 total aligned reads displayed.

CATCCGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTTCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAGGGC  >  NC_014034/2554258‑2554453
                                                                                                  |                                                                                                 
 aTCCGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAg                                                                                                >  1:241797/1‑101 (MQ=255)
   ccGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGcc                                                                                              >  2:70996/1‑101 (MQ=255)
   ccGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGcc                                                                                              <  1:3823553/101‑1 (MQ=255)
   ccGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGcc                                                                                              <  2:2796340/101‑1 (MQ=255)
    cGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGccc                                                                                             <  1:363485/101‑1 (MQ=255)
      cATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCtt                                                                                           <  2:282895/101‑1 (MQ=255)
      cATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCtt                                                                                           <  1:3924121/101‑1 (MQ=255)
       aTGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTc                                                                                          <  1:802990/101‑1 (MQ=255)
       aTGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTc                                                                                          <  2:4015757/101‑1 (MQ=255)
        tGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCg                                                                                         >  2:2101255/1‑101 (MQ=255)
         gTCCAAGGGCCCGTCGCCCAGAAAGGCAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCgg                                                                                        <  2:2111473/101‑1 (MQ=255)
          tCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCGGATCCAGCACCACCCCGTCCAGCCCTTCggg                                                                                       <  2:4545793/101‑1 (MQ=255)
          tCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCggg                                                                                       <  1:981500/101‑1 (MQ=255)
          tCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCggg                                                                                       >  2:740163/1‑101 (MQ=255)
             aaGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGgcgc                                                                                    >  1:4487059/1‑101 (MQ=255)
              aGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCGAGATTCAGCACCCTCCCGTCCACCACCTCTGGcgcg                                                                                   >  2:6468/1‑101 (MQ=255)
                 gCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGAtc                                                                                <  1:4000384/101‑1 (MQ=255)
                   ccGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGAtctc                                                                              <  1:545449/101‑1 (MQ=255)
                   ccGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGAtctc                                                                              <  1:3583788/101‑1 (MQ=255)
                   ccGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGAtctc                                                                              <  2:1107911/101‑1 (MQ=255)
                   ccGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGAtctc                                                                              >  2:3425934/1‑101 (MQ=255)
                    cGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcg                                                                             <  2:3387558/101‑1 (MQ=255)
                    cGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcg                                                                             >  1:2822932/1‑101 (MQ=255)
                     gTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcgg                                                                            >  2:3239844/1‑101 (MQ=255)
                      tCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcggc                                                                           >  2:3798471/1‑101 (MQ=255)
                       cgcgCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcggcg                                                                          >  1:2637155/1‑101 (MQ=255)
                          gcAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGgcggcg                                                                       <  2:2060593/101‑1 (MQ=255)
                          gcAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGgcggcg                                                                       <  2:3939434/101‑1 (MQ=255)
                           cAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGa                                                                      >  1:1609814/1‑101 (MQ=255)
                           cAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGa                                                                      <  1:41514/101‑1 (MQ=255)
                            ggAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGAc                                                                     <  2:2729550/100‑1 (MQ=255)
                            aGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGAc                                                                     >  2:2005376/1‑101 (MQ=255)
                             gAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGGGATCCCGgcggcgcgg                                                                    >  1:498261/1‑98 (MQ=255)
                             gAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACg                                                                    <  2:1650421/101‑1 (MQ=255)
                             gAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACg                                                                    <  2:4398407/101‑1 (MQ=255)
                             gAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGCCGCGATCTCGGCGGCGGCg                                                                    >  1:689880/1‑101 (MQ=255)
                                  gAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATc                                                               <  2:1226353/101‑1 (MQ=255)
                                  gAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATc                                                               <  1:3733831/101‑1 (MQ=255)
                                  gAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATc                                                               <  1:3018139/101‑1 (MQ=255)
                                   aaaaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCg                                                              <  2:3472359/101‑1 (MQ=255)
                                    aaaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGa                                                             >  2:3679764/1‑101 (MQ=255)
                                    aaaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGa                                                             <  1:3353727/101‑1 (MQ=255)
                                    aaaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGa                                                             <  1:3072201/101‑1 (MQ=255)
                                     aaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAg                                                            <  2:3638559/101‑1 (MQ=255)
                                      aGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAgg                                                           >  2:2658128/1‑101 (MQ=255)
                                         cgcgCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTc                                                        <  2:1495957/101‑1 (MQ=255)
                                           cgcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGa                                                      >  2:4067548/1‑101 (MQ=255)
                                           cgcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGa                                                      <  1:513536/101‑1 (MQ=255)
                                            gcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGaa                                                     >  1:1919004/1‑101 (MQ=255)
                                             cTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGaaa                                                    >  1:4373582/1‑101 (MQ=255)
                                              tCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGaaaa                                                   <  1:760723/101‑1 (MQ=255)
                                              tCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGaaaa                                                   >  2:2489715/1‑101 (MQ=255)
                                               cGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAg                                                  <  1:4548419/101‑1 (MQ=255)
                                                   cTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGgtgt                                              >  1:877560/1‑101 (MQ=255)
                                                    tGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGtt                                             <  1:1720028/101‑1 (MQ=255)
                                                    tGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGtt                                             >  2:2525367/1‑101 (MQ=255)
                                                    tGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGtt                                             <  2:1590218/101‑1 (MQ=255)
                                                     gATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTTGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTtc                                            >  1:1827468/1‑101 (MQ=255)
                                                     gATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTtc                                            <  1:4687231/101‑1 (MQ=255)
                                                      aTCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTtct                                           <  2:429342/101‑1 (MQ=255)
                                                        cGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCg                                         <  2:591954/101‑1 (MQ=255)
                                                        cGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGCCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTCCGAAAAGGGGTTCTCg                                         >  2:2198417/1‑101 (MQ=255)
                                                         gAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGa                                        >  1:200589/1‑101 (MQ=255)
                                                            cTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGGGTTCTCGacca                                     >  2:4637224/1‑101 (MQ=255)
                                                             tGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccag                                    <  2:1766004/101‑1 (MQ=255)
                                                             tGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccag                                    >  1:2333334/1‑101 (MQ=255)
                                                              gCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagc                                   <  1:4318013/101‑1 (MQ=255)
                                                              gCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagc                                   >  1:4286574/1‑101 (MQ=255)
                                                              gCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagc                                   >  2:332586/1‑101 (MQ=255)
                                                                aTCGACGACACGCCCAGATCCGGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcac                                 >  2:2310608/1‑101 (MQ=255)
                                                                aTCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcac                                 <  2:2591791/101‑1 (MQ=255)
                                                                  cgacgaCACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcacca                               <  2:4386749/101‑1 (MQ=255)
                                                                   gacgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcaccag                              <  1:3136141/101‑1 (MQ=255)
                                                                     cgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGcc                            <  1:2544367/101‑1 (MQ=255)
                                                                      gacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCg                           <  2:241772/101‑1 (MQ=255)
                                                                      gacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCg                           <  1:698946/101‑1 (MQ=255)
                                                                      cacccggccAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCg                           <  2:498210/94‑1 (MQ=255)
                                                                        cacGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCt                         >  2:3142304/1‑101 (MQ=255)
                                                                        cacGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCt                         >  2:4331184/1‑101 (MQ=255)
                                                                         acGCCCAGAGCCAGCACCCCCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTc                        <  2:932347/101‑1 (MQ=255)
                                                                           gCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGc                      <  2:2894490/101‑1 (MQ=255)
                                                                              cAGATCCAGCACCCCCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGa                   <  2:3753641/101‑1 (MQ=255)
                                                                               aGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGCTGTTCTCGACCAGCACCAGCCGCTCGCCGaa                  <  1:4203767/101‑1 (MQ=255)
                                                                                gATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAAc                 >  1:1355483/1‑101 (MQ=255)
                                                                                  tCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTc               <  1:2909104/101‑1 (MQ=255)
                                                                                     aGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTcgcc            >  1:4356184/1‑101 (MQ=255)
                                                                                     aGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTcgcc            >  1:2368001/1‑101 (MQ=255)
                                                                                     aGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTcgcc            >  1:3597721/1‑101 (MQ=255)
                                                                                      gCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTcgccg           >  2:1937555/1‑101 (MQ=255)
                                                                                       caccacCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGCACTcgccgc          >  1:2057345/1‑101 (MQ=255)
                                                                                       caccacCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAGCTcgccgc          <  2:304533/101‑1 (MQ=255)
                                                                                        ccccccccGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCcgcg         <  1:2862187/97‑1 (MQ=255)
                                                                                          caccCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGcc       >  2:4182642/1‑101 (MQ=255)
                                                                                          caccCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGcc       >  1:1034685/1‑101 (MQ=255)
                                                                                           cccccGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCa      <  1:1139195/100‑1 (MQ=255)
                                                                                           accCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCa      >  1:3801626/1‑101 (MQ=255)
                                                                                           accCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCa      >  1:174483/1‑101 (MQ=255)
                                                                                             cccGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAgg    >  1:2754671/1‑101 (MQ=255)
                                                                                             cccGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAgg    <  1:2247001/101‑1 (MQ=255)
                                                                                              ccGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAggg   <  1:3493357/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CATCCGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTTCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAGGGC  >  NC_014034/2554258‑2554453

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG