BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,559,923	G→C	100%	intergenic (‑199/+71)	ispZ ← / ← ftsY	intracellular septation protein A/cell division protein FtsY

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,559,923	0	G→C	100.0%	452.9	130	intergenic (‑199/+71)	ispZ/ftsY	intracellular septation protein A/cell division protein FtsY
Reads supporting (aligned to +/- strand): new base (119/8): ref base (0/3): total (119/11)

Only 100 of 173 total aligned reads displayed.


TCGCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGGGGGGGGCAGCGCCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGG  >  NC_014034/2559825‑2560022


tcgCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAACC.GCCGGGGGGGT.TCGCGTCC.GCGCAAgcgg                                                                                                    >  2:2487753/1‑101 (MQ=255)


tcgCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAgcgg                                                                                                    >  2:4218692/1‑101 (MQ=255)


     cGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGc                                                                                               >  2:1363193/1‑101 (MQ=255)


     cGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGc                                                                                               >  1:2092362/1‑101 (MQ=255)


     cGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGc                                                                                               >  2:3296766/1‑101 (MQ=255)


      ggTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCa                                                                                              >  1:3634259/1‑101 (MQ=255)


       gTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCAGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAg                                                                                             >  1:4049715/1‑101 (MQ=255)


        tGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAgc                                                                                            <  1:1463130/101‑1 (MQ=255)


         gCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAgcg                                                                                           <  1:3882916/101‑1 (MQ=255)


         gCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAgcg                                                                                           >  1:4555195/1‑101 (MQ=255)


          cGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAgcgc                                                                                          >  1:1636607/1‑101 (MQ=255)


           ggTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGcc                                                                                         >  2:1737871/1‑101 (MQ=255)


           ggTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGcc                                                                                         >  2:142104/1‑101 (MQ=255)


             tttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGcccc                                                                                       >  2:3151549/1‑101 (MQ=255)


             tttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGcccc                                                                                       >  1:4684694/1‑101 (MQ=255)


               tCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGcccccc                                                                                     >  2:2833728/1‑101 (MQ=255)


                 cTGACAGGATTGTTTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGCCA.GCGCAAGCGGGGGGCAGCGccccccga                                                                                   <  2:2854532/101‑3 (MQ=255)


                 cTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGccccccga                                                                                   >  2:2475159/1‑99 (MQ=255)


                 cTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGccccccga                                                                                   >  1:1871173/1‑99 (MQ=255)


                  tGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGccccccgaa                                                                                  >  1:3673363/1‑98 (MQ=255)


                   gACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGccccccgaag                                                                                 >  2:3824067/1‑97 (MQ=255)


                   gACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGccccccgaag                                                                                 >  2:515131/1‑97 (MQ=255)


                    aCAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCCCAAGCGGGGGGCAGCGcccccccaatc                                                                                >  2:3518652/1‑97 (MQ=255)


                     cAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAgcg                                                                             >  2:4627301/1‑101 (MQ=255)


                         aTTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTg                                                                         >  1:1968095/1‑101 (MQ=255)


                           tgtgTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAg                                                                       >  1:3236455/1‑101 (MQ=255)


                           tgtgTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAg                                                                       >  2:2534704/1‑101 (MQ=255)


                           tgtgTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAg                                                                       >  1:755574/1‑101 (MQ=255)


                           tggtcccTCCGCCCCGCGGCGGGCCCG.TTTTCCTTGGGGCAAAGC.GCGGGGGGGTG.GCGGGCCG.GCGAAGGGGGGGGGCAGC.CCCCCCC.GAAGCGCCTGAg                                                                       <  2:2656144/96‑1 (MQ=255)


                            gtgtCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAgg                                                                      >  1:2725848/1‑101 (MQ=255)


                               tCCTTCGCCCCGCAGCAGGCCCGCTTTTCCTG.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGgcgc                                                                   <  2:2291989/101‑1 (MQ=255)


                                ccTTCGTGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTTA.GCGCAAGCGGGGGGCAGCG.CGCCCC.GAAGGGCCTGAGGcgcg                                                                  >  2:1103806/1‑101 (MQ=255)


                                ccTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGcgcg                                                                  >  2:4693815/1‑101 (MQ=255)


                                ccTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGcgcg                                                                  >  1:2635951/1‑101 (MQ=255)


                                 ctccgccCCGCAGCGGCCCCGTTTTTCCTG.GGGCGAACC.GCGGGGGGGTT.GCGCGGCA.GCGCAGGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCgcgg                                                                 <  2:3084600/98‑1 (MQ=255)


                                 cTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCgcgg                                                                 >  2:2104864/1‑101 (MQ=255)


                                  ttCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCgcggc                                                                >  1:2560805/1‑101 (MQ=255)


                                    cgcgCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGcggcgg                                                              >  1:1743793/1‑101 (MQ=255)


                                      cgccgcAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGc                                                            >  1:42927/1‑101 (MQ=255)


                                      cgccgcAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGAAAGGGGGGGGCAGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGc                                                            <  2:2806209/101‑1 (MQ=255)


                                       gccgcAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCCCGGCGGGCt                                                           >  2:732903/1‑101 (MQ=255)


                                          gcagcaGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGGTTgc                                                        >  1:4409393/1‑101 (MQ=255)


                                          gcagcaGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgc                                                        >  2:2021750/1‑101 (MQ=255)


                                          cccggcggCCCCTTTTTCCTG.GGGGGAAAC.GGGGGGGGGGT.GCGGCGCG.GCCGAAGGGGGGGGGCGCCCCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTg                                                         <  1:2685097/95‑1 (MQ=255)


                                           cagcagGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgcc                                                       >  2:799794/1‑101 (MQ=255)


                                            agcagGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgccc                                                      >  2:1787428/1‑101 (MQ=255)


                                            agcagGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgccc                                                      >  1:3095176/1‑101 (MQ=255)


                                             gcagGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAACGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgcccg                                                     >  2:3143371/1‑101 (MQ=255)


                                              ggggCCCGTTTTTCC.T.GGGGGAAACGGCGGGGGGGTG.GCGGGGCG.GCGACGGGGGGGGGCAGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgcccgc                                                    <  1:4487960/99‑1 (MQ=12)


                                              cagGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGGCTgcccgc                                                    >  2:1679367/1‑101 (MQ=38)


                                              cagGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgcccgc                                                    >  2:4586927/1‑101 (MQ=255)


                                              cagGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCCTgcccgc                                                    >  2:52177/1‑101 (MQ=255)


                                                 gCCCTCTTTCCCTG.GGGAAAACC.GCGGGGGGTTG.GCGCGTCA.GCGAAGGCGGGGGGCGGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCa                                                 <  1:3051866/101‑1 (MQ=16)


                                                  cccGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgcccgcccca                                                >  1:3439683/1‑99 (MQ=255)


                                                        tttCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAgg                                          >  2:3417351/1‑101 (MQ=255)


                                                        tttCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCCTgaccgccccaaaaccg                                          >  2:719425/1‑93 (MQ=255)


                                                          tCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGgcg                                        >  1:4753880/1‑101 (MQ=255)


                                                            cTT.GGGCGAAAC.GCCGGGGGGTT.GCTCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCATGGCCGCCCAACGAAGGCGCa                                      >  1:1429203/1‑101 (MQ=255)


                                                             tt.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCaa                                     >  1:1525981/1‑101 (MQ=255)


                                                                gggGGAAAC.GGCGGGGGGTTGGCGCGTGA.GC.CAAGGGGGGGGGAAGCCCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAac                                    <  2:2001806/101‑1 (MQ=18)


                                                                    gAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCCACGAAAGCGCAACCCCCt                               >  2:3336501/1‑101 (MQ=25)


                                                                    gAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGGAGGCGCAACCACCt                               >  2:679215/1‑101 (MQ=255)


                                                                    gAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCt                               >  2:4047606/1‑101 (MQ=255)


                                                                    gAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCt                               >  1:100222/1‑101 (MQ=255)


                                                                    gAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCt                               >  1:4708248/1‑101 (MQ=255)


                                                                     agcc.GCGGGGGGGTG.GCGCGGCG.GCGAAGGGGGGGGGCGGC.CCCCCCG.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCtt                              <  1:560774/98‑1 (MQ=18)


                                                                      aaC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTc                             >  2:27473/1‑101 (MQ=255)


                                                                      aaC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCCTTCCCGCCCCACCAACGAGCAACCACCCTc                             >  1:2379909/1‑101 (MQ=255)


                                                                       accccgggggggTG.GCGCGCCG.GCGCAGGGGGGGGGC.CCCCCCCCCG.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTc                             <  1:3193483/95‑1 (MQ=18)


                                                                       aC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCa                            >  2:3008746/1‑101 (MQ=255)


                                                                         gggaggggggGT.GCGCGGCAGGCGAAGGGGGGGCGCA.CCCCCCCCC.AAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCa                            <  1:3823768/97‑1 (MQ=18)


                                                                           cgggggggTT.GCGCGTGA.GCGAAAGCGGGGGGCCGC.CCCCCAC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTc                         <  1:3143571/99‑1 (MQ=25)


                                                                            cGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACTAAGGCGCAACCACCTTCAGTCg                        >  1:4157067/1‑101 (MQ=255)


                                                                            cGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCg                        >  1:2284830/1‑101 (MQ=255)


                                                                             ggggggGG.GGGCGGCG.GCGGAAGGGGGGGGCCCCCCCCCCCG.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCg                        <  1:352065/101‑1 (MQ=21)


                                                                              gggggTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGGAGGCCGCACCGCCTTCAggccgc                      >  2:4223771/1‑96 (MQ=21)


                                                                                gggTT.GCGCGTCG.GCGAAGGGGGGGGGCGGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCtt                    <  2:441388/101‑1 (MQ=25)


                                                                                  gcgcgcgcGTCG.GCGC.GGGGGGGGGCCCCCCCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTc                   <  1:1309876/97‑1 (MQ=21)


                                                                                  gTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCCCCCAACGAAGGCGCAACCACCCTCAGTCGGCTTCg                  >  1:1687746/1‑101 (MQ=25)


                                                                                   tt.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCAACTTCAGTCCGCTTCGa                 >  2:2114904/1‑101 (MQ=25)


                                                                                    t.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGAt                >  1:4432477/1‑101 (MQ=255)


                                                                                    t.GCGCGCCG.GCG.AAGGGGGGGGCCGGCCCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGAt                <  1:2475302/101‑1 (MQ=25)


                                                                                         gcgccggcgcAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATccc             <  2:1501053/95‑1 (MQ=25)


                                                                                         cgTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATcccc            >  1:2378661/1‑101 (MQ=255)


                                                                                          gTCA.GCGCAAGCGGGGGGCAGCGCCCCCCC..AAGCGCCTGGGGCGCGGCGGGCCTCCCCGCCCAACGAAGGCGCACCCACCATCAGCCGGTTTAGATccccc           >  2:3967988/1‑100 (MQ=255)


                                                                                          gTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCcca           >  2:623235/1‑101 (MQ=255)


                                                                                          gTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCcca           >  1:4284615/1‑101 (MQ=255)


                                                                                           tCA.GCGCAAGCGGGGCGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTTCCCGCCCAACGAAGGCGCAACCACCTTCCGGCGGGTTCGATcccccc          >  2:1245083/1‑99 (MQ=255)


                                                                                             ggcgccgaGGGGGGGGGCCCGCCCCCCCGGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCcca           <  2:4152799/94‑1 (MQ=25)


                                                                                             ccgggcggggggggggGCGCCCCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccac          <  1:4627554/93‑1 (MQ=25)


                                                                                              gccgcggggggggggCAGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccacca        <  1:3897725/94‑1 (MQ=25)


                                                                                               cgcgagggggggggCCCCCCCCCCCG.AAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccacca        <  1:4269622/95‑1 (MQ=25)


                                                                                                 gcAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCCGTCGGCTTCGATCCCCACCAgcc     >  2:4662358/1‑100 (MQ=25)


                                                                                                  gggagggggggGAGGGCCCCCCCAGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgc      <  1:2019143/97‑1 (MQ=37)


                                                                                                  cAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCCCCAgcgc    >  2:2677176/1‑101 (MQ=255)


                                                                                                  cAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgcgc    >  1:2652264/1‑101 (MQ=255)


                                                                                                   aaGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGcgcg   >  2:710265/1‑101 (MQ=255)


                                                                                                   aaGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCAGCATCGATACCCACCAGcgcg   >  2:3769674/1‑101 (MQ=21)


                                                                                                    gggggggggCCCCCCCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGcgcg   <  1:1103892/100‑1 (MQ=255)


                                                                                                     ggggggggCCGCCGCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcgg  <  2:2725694/101‑1 (MQ=255)


TCGCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTT.GGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGGGGGGGGCAGCGCCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGG  >  NC_014034/2559825‑2560022

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 23 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG