BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,566,625	C→G	3.5%	intergenic (+97/+97)	purD → / ← sdaA	phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,566,625	0	C→G	3.5%	10.3 ‑3.6 ‑0.1	231	intergenic (+97/+97)	purD/sdaA	phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand): new base (8/0): ref base (136/86): total (144/87)
Fisher's exact test strand distribution p-value = 2.67e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.22e-01

Only 100 of 253 total aligned reads displayed.


AGACGAAAC.GGGGGCGCTGCCCCC.CTCCTG..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAACCGCCTC  >  NC_014034/2566528‑2566723


agaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGccc                                                                                                    <  2:1243486/101‑1 (MQ=21)


    gAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                                                                                                >  1:4378135/1‑101 (MQ=21)


    gAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                                                                                                >  2:4211402/1‑101 (MQ=21)


     aaacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAat                                                                                               >  1:4631004/6‑101 (MQ=21)


      aacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAata                                                                                              <  1:4113473/97‑1 (MQ=25)


       acgtggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCCCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAatat                                                                                             <  1:2573218/97‑1 (MQ=25)


       acggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAatat                                                                                             <  2:2619611/98‑1 (MQ=25)


       acggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAatat                                                                                             <  1:2995379/98‑1 (MQ=25)


        cagggggCGCTGCCCCCTCTTGGC..CTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggg                                                                                      >  2:2979150/3‑101 (MQ=0)


        cagggggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggg                                                                                      >  2:506050/3‑101 (MQ=0)


         ggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATcc                                                                                           >  2:1003043/2‑101 (MQ=25)


         ggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATcc                                                                                           <  2:3403742/100‑1 (MQ=25)


         agggggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggg                                                                                     >  1:2800801/2‑101 (MQ=0)


          gggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc                                                                                          <  2:3215671/101‑1 (MQ=25)


          gggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc                                                                                          >  2:1746777/1‑101 (MQ=25)


          gggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc                                                                                          <  1:942584/101‑1 (MQ=25)


           ggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCg                                                                                         <  1:4588220/101‑1 (MQ=25)


            gggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCgg                                                                                        <  1:4511503/101‑1 (MQ=37)


                  tGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGc                                                                                  <  1:3489476/101‑1 (MQ=37)


                        ccctcttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCt                                                                             <  1:1003795/91‑1 (MQ=255)


                         cctcttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCtt                                                                            <  1:3753939/92‑1 (MQ=255)


                         cctcttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCtt                                                                            <  1:402984/92‑1 (MQ=255)


                         cctcttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCtt                                                                            <  1:2133041/92‑1 (MQ=255)


                           tcttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgc                                                                          <  1:1211694/94‑1 (MQ=255)


                           tcttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgc                                                                          <  1:4260025/94‑1 (MQ=255)


                               ggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcgggc                                                                      >  1:2051894/4‑101 (MQ=255)


                                gccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcgggcg                                                                     <  1:2303930/99‑1 (MQ=255)


                                    tCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTTATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCggggg                                                                 >  1:1105181/1‑101 (MQ=255)


                                     cGGCCAACTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGc                                                                <  2:4520084/101‑1 (MQ=255)


                                      ggCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCa                                                               <  1:2102511/101‑1 (MQ=255)


                                      ggCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCa                                                               <  2:863892/101‑1 (MQ=255)


                                       gCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTTCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGcgg                                                              >  1:3666957/1‑99 (MQ=255)


                                       gCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGGAg                                                              >  2:2839463/1‑101 (MQ=255)


                                         cAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAgcg                                                            >  2:4290199/1‑101 (MQ=255)


                                          aaTTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGCGGGGGGCAgcgc                                                           >  2:2371746/1‑101 (MQ=255)


                                          aaTTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCCCTTGCGGGCGGGGGGAgcgc                                                           >  2:1851767/1‑101 (MQ=255)


                                             tCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcccc                                                        >  2:352185/1‑101 (MQ=255)


                                             tCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcccc                                                        >  1:2289366/1‑101 (MQ=255)


                                               aCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCg                                                      <  1:1581097/101‑1 (MQ=255)


                                                ccccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGa                                                     <  2:4464118/101‑1 (MQ=255)


                                                 cccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGaa                                                    <  2:2914325/101‑1 (MQ=255)


                                                     cGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAgcgc                                                <  2:740043/101‑1 (MQ=255)


                                                      gggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGcc                                               >  1:3865078/1‑101 (MQ=255)


                                                       ggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGccc                                              <  2:1578690/101‑1 (MQ=255)


                                                       ggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGccc                                              <  1:699301/101‑1 (MQ=255)


                                                          tatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                                           >  2:2695964/1‑101 (MQ=255)


                                                          tatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCCAAGCGCCCGAc                                           >  2:4477629/1‑101 (MQ=255)


                                                              tCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcgc                                       >  2:398864/1‑101 (MQ=255)


                                                                  gCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggcg                                   >  2:4402637/1‑101 (MQ=255)


                                                                    aaGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg                                 <  1:468968/101‑1 (MQ=255)


                                                                     aGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGc                                >  2:898488/1‑101 (MQ=255)


                                                                     aGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGc                                <  2:3585484/101‑1 (MQ=255)


                                                                     aGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGc                                >  1:3442773/1‑101 (MQ=255)


                                                                       aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt                              <  1:1864138/101‑1 (MQ=255)


                                                                       aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt                              >  2:634062/1‑101 (MQ=255)


                                                                       aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt                              >  1:1576416/1‑101 (MQ=255)


                                                                        tAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGGTTg                             >  1:3222290/1‑101 (MQ=255)


                                                                        tAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTg                             >  1:75682/1‑101 (MQ=255)


                                                                        tAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTg                             >  1:528397/1‑101 (MQ=255)


                                                                        tAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTg                             >  1:1800820/1‑101 (MQ=255)


                                                                         aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc                            >  2:2032335/1‑101 (MQ=255)


                                                                          aagaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTtcc                           >  2:1626063/1‑101 (MQ=255)


                                                                          aagaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcc                           >  2:3556561/1‑101 (MQ=255)


                                                                           agaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgccc                          >  2:1459285/1‑101 (MQ=255)


                                                                            gaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccg                         <  2:626484/101‑1 (MQ=255)


                                                                            gaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccg                         <  1:4163149/101‑1 (MQ=255)


                                                                             aagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgc                        <  2:3442566/101‑1 (MQ=255)


                                                                             aagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGATTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTtcccgc                        >  2:410070/1‑101 (MQ=255)


                                                                               ggTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgccc                      <  1:1746882/101‑1 (MQ=255)


                                                                                gTCTTTTCCTTTCATCTTGGCCGAAATATCCCGGGGGAGCCCGCTTGCAGGCGGGGGCAGCGCCCCCGAAGCGCCCGACTCGCGGCAGGCTTGCCCGCCCa                     <  1:2696118/101‑1 (MQ=39)


                                                                                gTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCa                     >  2:2248307/1‑101 (MQ=255)


                                                                                gTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCa                     >  1:1416460/1‑101 (MQ=255)


                                                                                 ttttttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCaa                    <  1:1883910/99‑1 (MQ=255)


                                                                                 tCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCaa                    >  1:1503155/1‑101 (MQ=255)


                                                                                  cTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAag                   >  1:1694673/1‑101 (MQ=255)


                                                                                  cTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAag                   <  1:1394771/101‑1 (MQ=255)


                                                                                  cTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAag                   >  1:1266699/1‑101 (MQ=255)


                                                                                  cTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAag                   >  1:4675741/1‑101 (MQ=255)


                                                                                   ttttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagg                  >  1:3006888/1‑101 (MQ=255)


                                                                                    tttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagga                 >  2:277423/1‑101 (MQ=255)


                                                                                    tttCCTTTCATCTTGCCCGAAATATCCAGGGGGAGTCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTAGCCCGCCCAaaga                 >  2:3168933/1‑101 (MQ=37)


                                                                                     ttCCTTTCATCTTGCCCGAAATATCGCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggag                <  1:2858601/101‑1 (MQ=255)


                                                                                     ttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCTaggag                >  2:1895315/1‑101 (MQ=255)


                                                                                       ccTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCCAGGAagg              >  2:1918207/1‑101 (MQ=255)


                                                                                       ccTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAggg              >  1:3841883/1‑101 (MQ=255)


                                                                                         tttCATCTTGCCCGAAATATCCCGGGGGCGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAACTCCCGACGCGCGGCGGGGTGGCCCGCCCAAGGAGGgcg            >  1:913522/1‑101 (MQ=38)


                                                                                          ttCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgcgc           >  2:2643496/1‑101 (MQ=255)


                                                                                          ttCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgcgc           >  1:2753538/1‑101 (MQ=255)


                                                                                           tCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCa          <  1:1485959/101‑1 (MQ=255)


                                                                                           tCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCa          >  1:3963066/1‑101 (MQ=255)


                                                                                            cATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCaa         >  2:4174101/1‑101 (MQ=255)


                                                                                            cATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCaa         >  2:2477641/1‑101 (MQ=255)


                                                                                             aTCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAAc        >  2:1984881/1‑101 (MQ=255)


                                                                                              tCTTCCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAAcc       <  2:1748997/101‑1 (MQ=255)


                                                                                               cTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAACCg      >  1:3048298/1‑101 (MQ=255)


                                                                                                 tGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAACCGcc    >  1:3537199/1‑101 (MQ=255)


                                                                                                 tGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAACCGcc    >  1:1672849/1‑101 (MQ=255)


                                                                                                 tGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAACCGcc    <  1:2427495/101‑1 (MQ=255)


                                                                                                   cccGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAACCGCCTc  >  2:1086348/1‑101 (MQ=255)


                                                                                                   cccGAAATACGCCGGGGGAGCCCGTTTGCGGGCGGCGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCACAAGGAGGGCGCAACCGCCTc  <  1:4477879/101‑1 (MQ=37)


AGACGAAAC.GGGGGCGCTGCCCCC.CTCCTG..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCAACCGCCTC  >  NC_014034/2566528‑2566723

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG