Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 257,090 C→G 100% intergenic (+24/‑175) secA → / → RCAP_rcc00221 preprotein translocase subunit SecA/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034257,0900C→G100.0% 688.0 185intergenic (+24/‑175)secA/RCAP_rcc00221preprotein translocase subunit SecA/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (87/98):  ref base (0/0):  total (87/98)

Only 100 of 201 total aligned reads displayed.

CCTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GCCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCGG  >  NC_014034/256996‑257187
                                                                                               |                                                                                                   
ccTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.Tcc                                                                                              <  1:3713270/101‑1 (MQ=255)
  tCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.gg                                                                                           <  2:1363231/101‑1 (MQ=255)
  tCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.gg                                                                                           <  1:2134369/101‑1 (MQ=255)
   cgcgCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.ggg                                                                                          <  1:4314101/101‑1 (MQ=255)
    gcgcAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGACTGAAACAAAGGCGCTGAAATATGGC.GGCGG.GCC.cggg                                                                                         >  1:2349905/1‑101 (MQ=255)
      gcAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTg                                                                                       <  1:2826990/101‑1 (MQ=255)
       cAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGa                                                                                      <  2:4601924/101‑1 (MQ=255)
         aTGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.GCC.cggggggaa                                                                                    >  1:4498672/1‑96 (MQ=255)
           gACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGG.GCC.gggggggaacc                                                                                  >  1:2421553/1‑94 (MQ=255)
           gACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAAccc                                                                                  <  1:2099680/101‑1 (MQ=255)
           gACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.GCC.gggggggaacc                                                                                  >  1:2769947/1‑94 (MQ=255)
              ccctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGG.GCG.GGGGGGAACCCgg                                                                                >  1:2270861/1‑101 (MQ=255)
               cctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGgc                                                                              <  2:3326565/101‑1 (MQ=255)
                ctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGgcc                                                                             <  1:1025207/101‑1 (MQ=255)
                   cccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGgccgcc                                                                          <  1:1245264/101‑1 (MQ=255)
                   cccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGgccgcc                                                                          <  1:341909/101‑1 (MQ=255)
                     cctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.GCC.GGGGGGGACCCCGGccgccgc                                                                        >  2:1774043/1‑100 (MQ=255)
                      ctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGG.GCC.GGGGGGGAACCCGGccgccggt                                                                       >  2:3545979/1‑99 (MQ=255)
                      cggcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGACCCCGGGCCGCCGtt                                                                       <  2:4045038/99‑1 (MQ=255)
                       tgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGttt                                                                      <  1:1650726/101‑1 (MQ=255)
                       tgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGttt                                                                      <  1:4353041/101‑1 (MQ=255)
                        gcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGG.GCC.GGGGGGACCCCGGCCCCCCGttt                                                                      >  2:4372603/1‑101 (MQ=255)
                        gcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGtttt                                                                     <  2:2744538/101‑1 (MQ=255)
                        gcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.GCCGGGGGGGAACCCGGG.CGCCGgttt                                                                     >  1:4166794/1‑101 (MQ=255)
                         cGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.GCC.GGGGGGGACCACGGgcgccgcgttt                                                                    >  2:3929042/1‑96 (MQ=255)
                          ggCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTc                                                                   <  1:218363/101‑1 (MQ=255)
                           gCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGG.TCC.GGGGGGGACCCGGGGCGCCCGTTTTCa                                                                  >  1:1404336/1‑101 (MQ=255)
                           gCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCa                                                                  <  1:1281210/101‑1 (MQ=255)
                           gCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.GCC.GGGGGGAGCCCGGGCCGCCGctttttt                                                                  >  2:832124/1‑99 (MQ=255)
                             tCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCAtt                                                                <  1:157465/101‑1 (MQ=255)
                             tCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCAtt                                                                <  1:230535/101‑1 (MQ=255)
                              cGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTa                                                               <  2:2336486/101‑1 (MQ=255)
                               gggCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGGGAAACCCGGCCCGCGCTTTTCATTAt                                                              >  1:3180775/1‑101 (MQ=255)
                               gggCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.GCC.GGGGGGAAACCCGGCCCCCGTTTTTCCtttt                                                              >  2:1154261/1‑99 (MQ=255)
                                  cGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.GCC.GGGGGGAACCCCGGCCGCCGGTTTTTCTTATCCt                                                           >  2:3461580/1‑101 (MQ=255)
                                   gagaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGTGG.TCC.CGGGGGAACCCGGGGCACCGGTTTTTATTATCCtt                                                          >  2:2331517/1‑100 (MQ=255)
                                    agaAGTTCAAGCACTGCCACGGTCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGG.GCC.GGGGGGAACCCGGGGCGCCGGTTTTCCTTATCCTGt                                                         >  1:2013017/1‑101 (MQ=255)
                                    agaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGt                                                         <  1:3339116/101‑1 (MQ=255)
                                    agaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGGGGACCCGGGCCGCCGTTTTTTATTTTCCttt                                                         >  1:3825189/1‑99 (MQ=255)
                                    agaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGGGGACCCCGGCCGCCGCTTTTTCTTATCCTGt                                                         >  2:3768802/1‑101 (MQ=255)
                                    agaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGGGAACCCGGGCCGCCGTTTTTCATTATTCTGt                                                         >  1:1223382/1‑101 (MQ=255)
                                     gaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGtt                                                        <  2:4496922/101‑1 (MQ=255)
                                     gaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGtt                                                        <  2:3063030/101‑1 (MQ=255)
                                     gaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.GCC.GGGGGGGAACCCGGCCGCCGGTTTTCAtttttctttt                                                        >  1:1901918/1‑93 (MQ=255)
                                      aaGTTTAAGAACTGTCAGGGCCGGCTGTGCGGAAGCAAAGGCGCTGAAGTATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTg                                                       <  2:657302/101‑1 (MQ=255)
                                      aaGTTCAAGCACTGCTACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTg                                                       <  2:3185492/101‑1 (MQ=255)
                                      aaGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTg                                                       <  1:2292717/101‑1 (MQ=255)
                                        gTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGAt                                                     <  2:1590922/101‑1 (MQ=255)
                                        gTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.GCC.GGGGGGAACCCGGGCCCCCGTTTTTCATTTTCCTTttttt                                                     >  2:1972665/1‑98 (MQ=255)
                                         ttCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGGGAACCCCGGGCGCCGCTTTTTTTTTTCCTGtttttt                                                    >  1:581317/1‑97 (MQ=255)
                                           cAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTg                                                  <  1:3852585/101‑1 (MQ=255)
                                           cAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.GCC.GGGGGGAACCCCGGCCGCCGGTTTTTATTTTTCTTTTGGTCTg                                                  >  1:926076/1‑101 (MQ=255)
                                            aaGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTgg                                                 <  1:3021510/101‑1 (MQ=255)
                                            aaGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTgg                                                 <  1:3173311/101‑1 (MQ=255)
                                             agaaCTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.GCC.GGGGGGAACCCCGGCCGCCGTTTTTCATTATCCTGTTGATCTggg                                                >  1:3509956/4‑100 (MQ=255)
                                             aGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGa                                                <  2:2533036/101‑1 (MQ=255)
                                             aGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGa                                                <  1:4480316/101‑1 (MQ=255)
                                              gCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGGGAACCCGGGGCGCCGGTTTTTATTATTCTGTTGATCTGGat                                               >  2:3654356/1‑101 (MQ=255)
                                              gCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGGGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTgggt                                               >  1:3206665/1‑99 (MQ=255)
                                               cACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.GCC.GGGGGGAACCCGGGCCGCCGGTTTTTATTATCCTTTTGATCTGGgta                                              >  1:3273243/1‑101 (MQ=255)
                                                aCTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGatat                                             <  2:4413192/101‑1 (MQ=255)
                                                 cTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATg                                            <  1:370108/101‑1 (MQ=255)
                                                 cTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATg                                            <  1:1120298/101‑1 (MQ=255)
                                                   gCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGtt                                          <  1:2962683/101‑1 (MQ=255)
                                                    ccACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGACCCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGttt                                         <  2:2104856/101‑1 (MQ=255)
                                                     cccGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGtttt                                        <  1:882101/99‑1 (MQ=255)
                                                     cACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGtttt                                        <  1:1774152/101‑1 (MQ=255)
                                                       cGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTtg                                      <  1:1235541/101‑1 (MQ=255)
                                                        ggCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGG.TCC.GGGGGGAACCCGGGGCCCCGGTTTTCCTTGTACCACTGATCTGGGTTTGttttttt                                     >  2:4550862/1‑99 (MQ=255)
                                                           cGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGa                                  <  1:459554/101‑1 (MQ=255)
                                                           cGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGa                                  <  2:4199325/101‑1 (MQ=255)
                                                            gTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGCTCG.GGGGGGAACCGGGGCGCCCCTTTTTC.TTATCCTTTTCGGCTGGCAATGTTTTTTTGGaa                                 >  1:249980/1‑101 (MQ=255)
                                                            gTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGaa                                 <  2:4498422/101‑1 (MQ=255)
                                                             tCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGaaa                                <  1:2849817/101‑1 (MQ=255)
                                                             tCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGGGAACCCGGGCCGCCGTTTTTCATTGTCCTGTTGATCTGGATATGTTTTTTTGGaaa                                >  2:620772/1‑101 (MQ=255)
                                                             tCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.GCC.GGGGGGGAACCGGGCCGCCGGTTTTTATTTTTCCGTTTATCTTGGTATGTTTTTTTGGgaa                                >  1:3979606/1‑101 (MQ=255)
                                                              cTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAAt                               <  2:1901806/101‑1 (MQ=255)
                                                              cTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAAt                               <  2:2249915/101‑1 (MQ=255)
                                                               tGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TTC.GGGGGGAACCCCGGGCGCCGTTTTTTATTTTCCTGTTTATTTTGATATGTTTTTGTggtcatt                              >  1:3446660/1‑96 (MQ=255)
                                                               tGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAAtt                              <  1:3769037/101‑1 (MQ=255)
                                                               tGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAAtt                              <  2:125509/101‑1 (MQ=255)
                                                                  ccTGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTcc                           <  2:240776/101‑1 (MQ=255)
                                                                    tGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTccgg                         <  2:2834753/101‑1 (MQ=255)
                                                                    tGAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGGGAACCCGGGCCGCCGTTTTTTATTATCCTGTTGATCTGGATATGTTTTTTTGGAAATTTccgg                         >  1:4024818/1‑101 (MQ=255)
                                                                     gAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTTTGGAAATTTccggc                        >  1:2209215/1‑101 (MQ=255)
                                                                     gAAACAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTccggc                        <  1:3546198/101‑1 (MQ=255)
                                                                      aaaCAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGGGAACCCGGGCCGCCGTTTTTTATTATCCTGTTGATCTGGATATGTTTTTTTGGAAATTTccggcc                       >  1:2784540/1‑101 (MQ=255)
                                                                      aaaCAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGGGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGGTATGTTTTTTTTGAAATTTccggcc                       >  2:2366859/1‑101 (MQ=255)
                                                                        aCAAAGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTccggccgg                     <  2:2670615/101‑1 (MQ=255)
                                                                          aaaGGCGCTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCCGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTTTGGAAAATTCCGGCCCGGc                   >  2:2356305/1‑101 (MQ=255)
                                                                            aGGCGCTGAAATATGGC.GGCGG.TCC.GGGGGGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTTTGGAAATTTCCGGCCGGGCGa                 >  1:804255/1‑101 (MQ=255)
                                                                              gctcTGAAATATGGC.GGCGG.TCC.GGGTTGATCCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAg               <  2:581266/98‑1 (MQ=255)
                                                                               cgcTGAAATATGGC.GGCGG.TCC.GGGGTGAACCCCGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATAAGTTTTTGTGGAAATTTCCGGCCGGGCGAAgg              <  1:4206460/101‑1 (MQ=255)
                                                                                   gAAATATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTc          <  2:2499449/101‑1 (MQ=255)
                                                                                    aaaTATGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTcc         <  1:1180149/101‑1 (MQ=255)
                                                                                    aaaTATGGC.GGCGG.TCC.GGGGGGAACCCGGGCCGCCGGTTTTTATTATCCTGTTGATCTGGATATGTTTTTTTGGAAATTTCCCGCCGGGCGGAGGGTTcc         >  2:1652158/1‑101 (MQ=255)
                                                                                     aaTAGGGC.GGCGG.TCC.GGGGTGAACCCGGCCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCg        <  2:2012900/101‑1 (MQ=255)
                                                                                       tatGGC.GGCGG.TCC.GGGGGGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCCGGCGAAGGTTTCCGtt      >  2:1445553/1‑101 (MQ=255)
                                                                                        atGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGttt     <  1:709358/101‑1 (MQ=255)
                                                                                         tGGC.GGCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTc    >  2:4017003/1‑101 (MQ=255)
                                                                                               |                                                                                                   
CCTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GCCGG.TCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCGG  >  NC_014034/256996‑257187

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG