Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,579,493 A→C 100% S227A (TCC→GCC)  pgi ← glucose‑6‑phosphate isomerase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,579,4930A→C100.0% 780.4 208S227A (TCC→GCC) pgiglucose‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  new base (108/100):  ref base (0/0):  total (108/100)

Only 100 of 221 total aligned reads displayed.

ACCGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGAGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTGGACGC  >  NC_014034/2579396‑2579589
                                                                                                 |                                                                                                
 ccGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAAt                                                                                              <  2:2590332/101‑1 (MQ=255)
   gAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTg                                                                                            <  1:1621617/101‑1 (MQ=255)
    aaTAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTgc                                                                                           >  2:3879637/1‑101 (MQ=255)
     aTAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTgcg                                                                                          <  1:1788742/101‑1 (MQ=255)
       aGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGcgcc                                                                                        >  1:1831757/1‑101 (MQ=255)
        gcgcCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGcgccg                                                                                       <  1:3598420/101‑1 (MQ=255)
        gcgcCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCCCGGTCGGGATCGCGGCGAATTGcgccc                                                                                       >  2:2280427/1‑100 (MQ=255)
          gcccgcccACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCgccgcc                                                                                     >  1:3155335/1‑101 (MQ=255)
          gcccgcccACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCgccgcc                                                                                     >  1:262263/1‑101 (MQ=255)
            ccgcccACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCgg                                                                                   >  2:823952/1‑101 (MQ=255)
              gcccACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGt                                                                                 >  1:4422413/1‑101 (MQ=255)
                ccacccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTcc                                                                               >  1:4241440/1‑101 (MQ=255)
                  acccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGt                                                                             >  1:264258/1‑101 (MQ=255)
                   cccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTg                                                                            <  2:2330437/101‑1 (MQ=255)
                   cccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTg                                                                            <  2:2850299/101‑1 (MQ=255)
                     caATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGAc                                                                          <  2:3284455/101‑1 (MQ=255)
                      aaccctgaaaGCCGAAGACGCGCTCGGGCGGGAGGCCGAAAGCCGCCGTCCTGTCGGGCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGAcc                                                                         <  1:1028839/93‑1 (MQ=255)
                      aaTCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGAcc                                                                         <  2:1596995/101‑1 (MQ=255)
                      aaTCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGAcc                                                                         <  2:4475269/101‑1 (MQ=255)
                      aaTCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGAcc                                                                         >  1:2934724/1‑101 (MQ=255)
                       aTCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCt                                                                        <  2:2201588/101‑1 (MQ=255)
                        tCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCtt                                                                       >  2:4347620/1‑101 (MQ=255)
                         ccTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTc                                                                      <  1:855876/101‑1 (MQ=255)
                         ccTCGAAGCCGAAGACGCGCTCGGGCGGGAGGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCAGCGAATTGCGCTGCCGGGCCCGTGATCTTc                                                                      >  1:4225507/1‑101 (MQ=255)
                          cTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcg                                                                     >  1:1265023/1‑101 (MQ=255)
                          cTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcg                                                                     <  2:4536816/101‑1 (MQ=255)
                           tCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcgc                                                                    >  2:1798121/1‑101 (MQ=255)
                           tCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcgc                                                                    >  2:993460/1‑101 (MQ=255)
                            cGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcgcc                                                                   >  1:3895930/1‑101 (MQ=255)
                             gAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcgccg                                                                  <  1:130858/101‑1 (MQ=255)
                             gAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcgccg                                                                  <  2:262238/101‑1 (MQ=255)
                             gAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcgccg                                                                  >  1:3314395/1‑101 (MQ=255)
                               aGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCgccgcc                                                                >  1:2098171/1‑101 (MQ=255)
                                 ccGAAGACGCGCGGGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCAt                                                              <  2:3318435/101‑1 (MQ=255)
                                  cGAAGACGCGCTCGGGCGGGATGGCGAAAGCCGCCGTCCTGTCGGTCACGGTCGAAATCGAGGCGAATTGCCCCGCCGGGTACGTGACCTTCGCCGCCTTc                                                             >  2:763740/1‑101 (MQ=255)
                                   gAAGANNCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATcc                                                            <  1:1628999/101‑1 (MQ=255)
                                   gAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATcc                                                            <  2:3313428/101‑1 (MQ=255)
                                     aGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAg                                                          >  2:1546152/1‑101 (MQ=255)
                                       aCGCGGTCGGGCGGGAGGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGgc                                                        <  2:1300551/101‑1 (MQ=255)
                                          cgcTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGcgcg                                                     >  1:230235/1‑101 (MQ=255)
                                           gcTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGACgcgg                                                    <  1:1050992/101‑1 (MQ=255)
                                              cgggcgggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCgcgggcg                                                 >  2:3661922/1‑101 (MQ=255)
                                               gggcgggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCgcgggcgg                                                >  2:919817/1‑101 (MQ=255)
                                                 gcgggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGtt                                              <  2:2983967/101‑1 (MQ=255)
                                                  cgggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGttt                                             <  2:3452393/101‑1 (MQ=255)
                                                  cgggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGttt                                             <  2:711893/101‑1 (MQ=255)
                                                  cgggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGttt                                             <  2:1754409/101‑1 (MQ=255)
                                                   gggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTc                                            <  2:1112199/101‑1 (MQ=255)
                                                    ggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCg                                           >  1:653384/1‑101 (MQ=255)
                                                     gATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCgg                                          >  1:4299010/1‑101 (MQ=255)
                                                     gATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCgg                                          >  1:2234563/1‑101 (MQ=255)
                                                       tGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCa                                        >  1:1867447/1‑101 (MQ=255)
                                                       agcggagaGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCa                                        <  2:3828996/94‑1 (MQ=255)
                                                         ccGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCAtt                                      >  2:2043960/1‑101 (MQ=255)
                                                          cGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTg                                     >  2:2644449/1‑101 (MQ=255)
                                                            aaaGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGt                                   >  1:3630969/1‑101 (MQ=255)
                                                            aaaGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGt                                   <  2:937075/101‑1 (MQ=255)
                                                            aaaGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGt                                   >  2:1507317/1‑101 (MQ=255)
                                                             aaGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCAAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTc                                  <  2:3174260/101‑1 (MQ=255)
                                                               gccgccGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCAt                                >  2:1680244/1‑101 (MQ=255)
                                                               gccgccGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCAt                                <  2:1356152/101‑1 (MQ=255)
                                                                ccgccgTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATc                               >  1:2769639/1‑101 (MQ=255)
                                                                ccgccgTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATc                               <  2:2439725/101‑1 (MQ=255)
                                                                  gccgTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGt                             >  2:2946226/1‑101 (MQ=255)
                                                                     gTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGtctc                          >  1:1744177/1‑101 (MQ=255)
                                                                     gTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGtctc                          <  2:12314/101‑1 (MQ=255)
                                                                     gTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGtctc                          <  2:3558515/101‑1 (MQ=255)
                                                                     gTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGtctc                          <  1:688963/101‑1 (MQ=255)
                                                                     gTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGCCATTGGTCATCGtctc                          >  2:3111370/1‑101 (MQ=255)
                                                                       ccTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGa                        >  2:727031/1‑101 (MQ=255)
                                                                       ccTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGa                        >  1:1400812/1‑101 (MQ=255)
                                                                       ccTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGa                        >  2:892815/1‑101 (MQ=255)
                                                                       ccTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTAGa                        >  2:4002709/1‑101 (MQ=255)
                                                                        cTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGAt                       <  1:214509/101‑1 (MQ=255)
                                                                        cTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGAt                       >  1:1686587/1‑101 (MQ=255)
                                                                         tGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGCTc                      <  1:2707061/101‑1 (MQ=255)
                                                                         tGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATc                      <  1:1228198/101‑1 (MQ=255)
                                                                         tGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATc                      >  2:2880603/1‑101 (MQ=255)
                                                                         tGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATc                      <  1:1504782/101‑1 (MQ=255)
                                                                         tGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCAATGGTCATCGTCTCGATc                      >  2:4268527/1‑101 (MQ=255)
                                                                          gtcggtcgCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCg                     >  1:2415362/1‑101 (MQ=255)
                                                                          gtcggtcgCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCg                     >  2:2689386/1‑101 (MQ=255)
                                                                          gtcggtcgCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCg                     <  1:716521/101‑1 (MQ=255)
                                                                             ggtcgcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgtgg                  >  1:3752994/1‑101 (MQ=255)
                                                                              gtcgcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgtggt                 >  2:4065277/1‑101 (MQ=255)
                                                                                cgcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGa               <  2:1064386/101‑1 (MQ=255)
                                                                                  cggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAg             >  1:350304/1‑101 (MQ=255)
                                                                                  cggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAg             >  1:1082327/1‑101 (MQ=255)
                                                                                  cggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAg             >  2:369917/1‑101 (MQ=255)
                                                                                   ggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAgg            >  2:4719307/1‑101 (MQ=255)
                                                                                   ggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAgg            <  1:203534/101‑1 (MQ=255)
                                                                                     tcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCTATCGTGGTGAAGGTc          <  1:4002951/101‑1 (MQ=255)
                                                                                     tcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTc          <  2:1867335/101‑1 (MQ=255)
                                                                                      cgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCt         >  2:594596/1‑101 (MQ=255)
                                                                                        agaTCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTg       <  1:994733/101‑1 (MQ=255)
                                                                                         gaTCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTgg      <  2:3379750/101‑1 (MQ=255)
                                                                                         gaTCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTgg      <  1:3141583/101‑1 (MQ=255)
                                                                                         gaTCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTgg      >  1:539995/1‑101 (MQ=255)
                                                                                           tCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTGGAc    <  2:2759940/101‑1 (MQ=255)
                                                                                             gcgGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTGGACGc  >  2:2125515/1‑101 (MQ=255)
                                                                                                 |                                                                                                
ACCGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGAGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTGGACGC  >  NC_014034/2579396‑2579589

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG