Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,590,068 C→G 100% V12L (GTT→CTT)  glnE ← glutamate‑ammonia‑ligase adenylyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,590,0680C→G100.0% 661.2 175V12L (GTT→CTT) glnEglutamate‑ammonia‑ligase adenylyltransferase
Reads supporting (aligned to +/- strand):  new base (84/91):  ref base (0/0):  total (84/91)

Only 100 of 186 total aligned reads displayed.

CGAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAACGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTT  >  NC_014034/2589970‑2590165
                                                                                                  |                                                                                                 
cGAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGgg                                                                                                 >  2:4091401/1‑101 (MQ=255)
 gAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGggg                                                                                                >  1:189163/1‑101 (MQ=255)
     aGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTg                                                                                            <  1:2634281/101‑1 (MQ=255)
        ccgccgTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCgg                                                                                         >  2:2768876/1‑101 (MQ=255)
         cgccgTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCggg                                                                                        >  1:4487140/1‑101 (MQ=255)
           ccgTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGAAATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTc                                                                                      <  1:2375623/101‑1 (MQ=255)
             gTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAg                                                                                    <  1:103958/101‑1 (MQ=255)
             gTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAg                                                                                    <  2:1801907/101‑1 (MQ=255)
              tGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAgg                                                                                   >  1:2510120/1‑101 (MQ=255)
               gCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGc                                                                                  <  2:3710914/101‑1 (MQ=255)
                    cgATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGAt                                                                             <  2:368562/101‑1 (MQ=255)
                    cgATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGAt                                                                             <  2:4340728/101‑1 (MQ=255)
                    cgATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGAt                                                                             <  1:342645/101‑1 (MQ=255)
                    cgATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGAt                                                                             >  2:4053127/1‑101 (MQ=255)
                     gATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATg                                                                            >  1:3977510/1‑101 (MQ=255)
                     gATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATg                                                                            >  1:3276127/1‑101 (MQ=255)
                     gATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATg                                                                            <  2:4223464/101‑1 (MQ=255)
                      aTCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGc                                                                           >  2:2603951/1‑101 (MQ=255)
                       tCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCa                                                                          >  1:4708534/1‑101 (MQ=255)
                       tCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCa                                                                          <  1:4456950/101‑1 (MQ=255)
                        cAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCaa                                                                         >  2:1453639/1‑101 (MQ=255)
                        cAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCaa                                                                         >  1:767666/1‑101 (MQ=255)
                        cAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCaa                                                                         >  1:3546357/1‑101 (MQ=255)
                         aaCCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAg                                                                        >  1:3050169/1‑101 (MQ=255)
                         aaCCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAg                                                                        >  2:538788/1‑101 (MQ=255)
                          aCCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAgg                                                                       <  1:3057716/101‑1 (MQ=255)
                           cccccGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGc                                                                      <  2:3913271/101‑1 (MQ=255)
                              ccGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCa                                                                   >  2:3020971/1‑101 (MQ=255)
                               cGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCAt                                                                  <  1:3645514/101‑1 (MQ=255)
                               cGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCAt                                                                  <  1:2248000/101‑1 (MQ=255)
                                 gATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATgg                                                                <  1:3365195/101‑1 (MQ=255)
                                  aTTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGgc                                                               >  2:3485923/1‑101 (MQ=255)
                                   tttCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGgcg                                                              >  1:3826774/1‑101 (MQ=255)
                                   tttCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGgcg                                                              <  1:2604103/101‑1 (MQ=255)
                                      cGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCa                                                           >  2:1920007/1‑101 (MQ=255)
                                      cGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCa                                                           >  2:2091423/1‑101 (MQ=255)
                                       gggCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAg                                                          <  2:3244162/101‑1 (MQ=255)
                                        ggCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGa                                                         >  1:3938998/1‑101 (MQ=255)
                                          cGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACt                                                       <  1:4603618/101‑1 (MQ=255)
                                           ggAAAATCGCCATAACGGGCAACGACATCGGCGCTACGGGCCGGATCAAGGGGAAGCGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCTCCAGAcct                                                      >  2:289474/1‑99 (MQ=255)
                                           ggAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTa                                                      <  1:3382893/101‑1 (MQ=255)
                                           ggAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTa                                                      >  1:1127870/1‑101 (MQ=255)
                                            gAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAg                                                     >  2:3851877/1‑101 (MQ=255)
                                             aaaaTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAgc                                                    >  2:4051181/1‑101 (MQ=255)
                                             aaaaTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAgc                                                    >  1:267255/1‑101 (MQ=255)
                                              aaaTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAgcc                                                   >  2:3602836/1‑101 (MQ=255)
                                                     cATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCaa                                            <  2:4723585/101‑1 (MQ=255)
                                                       tAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGc                                          <  2:30475/101‑1 (MQ=255)
                                                       tAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGAGCCAGACTAGCCGCCGCAAGc                                          >  1:2026988/1‑101 (MQ=255)
                                                         aCGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGccc                                        >  2:3443664/1‑101 (MQ=255)
                                                         aCGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGccc                                        >  1:4424681/1‑101 (MQ=255)
                                                         aCGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGccc                                        >  2:2140037/1‑101 (MQ=255)
                                                          cGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGcccc                                       <  1:4026435/101‑1 (MQ=255)
                                                           gggCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCg                                      >  2:4425913/1‑101 (MQ=255)
                                                            ggCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCgg                                     >  2:3188154/1‑101 (MQ=255)
                                                            ggCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCgg                                     >  2:3000736/1‑101 (MQ=255)
                                                             gCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCggg                                    <  2:1758734/101‑1 (MQ=255)
                                                               aaCGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTc                                  >  2:4573587/1‑101 (MQ=255)
                                                               aaCGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTc                                  >  2:1595323/1‑101 (MQ=255)
                                                                 cGACATCGGCGCCAGGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCaa                                <  2:186861/101‑1 (MQ=255)
                                                                 cGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCaa                                <  1:4248929/101‑1 (MQ=255)
                                                                 cGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCaa                                <  1:167666/101‑1 (MQ=255)
                                                                  gACATCGGCGCCAGGGGCCGGATCAAGGGGAAGTGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAg                               <  1:4091643/101‑1 (MQ=255)
                                                                  gACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAg                               >  1:638148/1‑101 (MQ=255)
                                                                  gACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAg                               >  2:2275495/1‑101 (MQ=255)
                                                                  gACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAg                               >  2:2413801/1‑101 (MQ=255)
                                                                  gACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAg                               <  2:3571926/101‑1 (MQ=255)
                                                                   aCATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGTGCCAGCCTAGCCCCCGCAAGCTCGGAGTCAAgg                              >  2:2373017/1‑101 (MQ=255)
                                                                     aTCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGgcg                            >  1:4340136/1‑101 (MQ=255)
                                                                     aTCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGgcg                            >  1:1048358/1‑101 (MQ=255)
                                                                     aTCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGgcg                            <  1:1528745/101‑1 (MQ=255)
                                                                         gcgcCCCGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCa                        <  1:1827270/101‑1 (MQ=255)
                                                                            ccACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAAt                     <  2:3826539/101‑1 (MQ=255)
                                                                            ccACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAAt                     <  2:2508847/101‑1 (MQ=255)
                                                                            ccACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAAt                     <  2:672381/101‑1 (MQ=255)
                                                                               cGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgcc                  <  2:267230/101‑1 (MQ=255)
                                                                                gggCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccc                 >  2:4674718/1‑101 (MQ=255)
                                                                                gggCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccc                 >  2:2144631/1‑101 (MQ=255)
                                                                                gggCCGGATCAAGGGGAAGGGGCGTGAGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccc                 >  2:1429564/1‑101 (MQ=255)
                                                                                gcgCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGGTGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAACGCGCGCAACTgccc                 >  2:4152750/3‑101 (MQ=255)
                                                                                 ggCCGGCTCACGGGGAGGGGGGTTGCGGGTCAGGCGGGATGCAAGACTCATGGCCCCAGCCTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgcccc                <  2:558200/101‑1 (MQ=255)
                                                                                 ggCCGGATCAAGGGGAGGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgcccc                <  1:582727/101‑1 (MQ=255)
                                                                                 ggCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGTCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgcccc                <  1:4051423/101‑1 (MQ=255)
                                                                                 ggCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGGTGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCACATgcccc                >  1:2993402/1‑101 (MQ=255)
                                                                                  gcgggACCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccg               <  2:3435404/98‑1 (MQ=255)
                                                                                  gCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccc               >  1:894369/1‑100 (MQ=255)
                                                                                   cgggATGAAGGGGAAGGGGCGTGCGTGTCGGGTGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccgc              <  2:1643510/99‑1 (MQ=255)
                                                                                      gATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccgcccc           >  2:3370995/1‑101 (MQ=255)
                                                                                      gATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccgcccc           >  1:2770054/1‑101 (MQ=255)
                                                                                       aTCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCt          <  2:1413301/101‑1 (MQ=255)
                                                                                       aTCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCt          >  2:30836/1‑101 (MQ=255)
                                                                                       aTCAAGAGGAAGGGGGGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCt          <  1:776912/101‑1 (MQ=255)
                                                                                        tCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTg         <  1:1448949/101‑1 (MQ=255)
                                                                                         cAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGt        >  1:1871843/1‑101 (MQ=255)
                                                                                         cAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGt        >  2:665798/1‑101 (MQ=255)
                                                                                          aaGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCACCGCCCCTGTc       >  2:115716/1‑101 (MQ=255)
                                                                                          aaGGGGAAGGGGCGGGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTc       <  1:3603055/101‑1 (MQ=255)
                                                                                           aGGGGAGGGGGCGTGCGGGTCAGGCGGGATGCAAGCCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCa      <  2:3191978/101‑1 (MQ=255)
                                                                                            ggggAAGGGGCGTGGGGGGCAGGCGGGATGCAAGGCTCATGGCGCCAGCCTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCAt     <  2:4609860/101‑1 (MQ=255)
                                                                                               gAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCtt  <  2:1257223/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CGAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAACGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTT  >  NC_014034/2589970‑2590165

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG