Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,603,050	A→C	100%	E160A (GAG→GCG)	RCAP_rcc02427 →	M23 family peptidase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,603,050	0	A→C	100.0%	96.8	26	E160A (GAG→GCG)	RCAP_rcc02427	M23 family peptidase
Reads supporting (aligned to +/- strand):  new base (13/13):  ref base (0/0):  total (13/13)

CGGGCGGGGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGAGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCGCC  >  NC_014034/2602954‑2603141                                                                                                 |                                                                                            ggggggggGCTCCCGCCGCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACg                                                                                         <  2:593214/100‑1 (MQ=255) ggggcgggGCGGCCCCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCGGCGATCGACCGCGCCGCCGTCCCCCAGCCGCCCGCCGCGACg                                                                                         <  2:3399657/100‑1 (MQ=255)  gggcgggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGc                                                                                        <  2:1132120/101‑1 (MQ=255)     gggggCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCcg                                                                                     <  1:4747283/100‑1 (MQ=255)            gccgccGCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGcccc                                                                              <  1:4479079/101‑1 (MQ=255)             ccgccgCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGccccc                                                                             <  2:481292/101‑1 (MQ=255)                ccgcGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGcc                                                                          <  2:1603908/101‑1 (MQ=255)                 cgcgGGCGGCGAGCGGATCGACACCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCa                                                                         <  2:968816/101‑1 (MQ=255)                   ggggCAGCGAGGGGATCGACATCCCCCCGCTGGGGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCAcc                                                                       <  2:4656704/100‑1 (MQ=255)                            agccGACCGACATCCCCGCCTTGGCGGGGGCCAAGACCAGCCGCCCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGt                                                              <  2:2445714/101‑1 (MQ=255)                                  tCGACATCACCTCGCTGGCGGGGGGCGCGCGCGGCCGCGCCGCCCTCCCCCACCCGCCCGCCCCCCCGCCCGCGGCCCCCGCCCCCCCCCCCCCCCccgcc                                                        >  2:4111322/1‑101 (MQ=255)                                           ccccGCTGGGGGGGGCCGCGACCCCCCGCGCCCCCGCCCCCCAGCCCCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGt                                               <  1:450413/98‑1 (MQ=255)                                                 tGGCGGGGGGGGCGAGCGACCGCGCCGCCGTCACCCAGCCCCCCCCCCCGCCGCCCCCCGGCCCCCCCCCCGCGCCCCCCCCCGCCGCGcccaaagcccgc                                         >  2:3925981/1‑91 (MQ=255)                                                      ggggCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGt                                    >  2:2003157/1‑101 (MQ=255)                                                             cgATCGCCCGCGCCGCCGCCCCCCAGCCCCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGaaa                             <  2:3472119/101‑1 (MQ=255)                                                               aTCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAAcc                           >  1:3598423/1‑101 (MQ=255)                                                                 cGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCgg                         >  2:4511081/1‑101 (MQ=255)                                                                        gccgccGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGccgcccc                  >  2:728251/1‑101 (MQ=255)                                                                         ccgcccccccccCGCCCCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGccgccccc                 <  1:3926223/92‑1 (MQ=255)                                                                           gccgTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGccgcccccgc               >  1:1626459/1‑101 (MQ=255)                                                                           gccgTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAATGCCAGCCCGGTGGCGGAACCGGccgcccccgc               >  2:4489450/1‑101 (MQ=255)                                                                             cgTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGccgcccccgccc             >  1:1194593/1‑101 (MQ=255)                                                                              gccccccGGCCCCCCGCCGCGCCGCCCGCGGCCCCCGCCCCCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCa            <  1:4111564/97‑1 (MQ=255)                                                                               tCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAg           >  1:497681/1‑101 (MQ=255)                                                                               tCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAg           >  2:4690224/1‑101 (MQ=255)                                                                                  cccAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGAcc        >  1:4306778/1‑101 (MQ=255)                                                                                    cAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCgg      >  1:228415/1‑101 (MQ=255)                                                                                    cAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGCGGCGAAACCGGCCGCCCCCGCCCAGACCgc      >  2:45141/1‑100 (MQ=255)                                                                                        cgcccgccGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCgcc  >  1:3553318/1‑101 (MQ=255)                                                                                        cccccgccGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCgcc  <  1:2003274/99‑1 (MQ=255)                                                                                                 |                                                                                            CGGGCGGGGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGAGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCGCC  >  NC_014034/2602954‑2603141 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG