Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,209 A→T 13.4% intergenic (‑185/+91) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2090A→T13.4% 63.7 ‑0.4 0.0179intergenic (‑185/+91)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (12/12):  ref base (85/69):  total (98/81)
Fisher's exact test strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

AACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGG  >  NC_014034/2640111‑2640309
                                                                                                    |                                                                                                    
 aCCGGGGCGCTTTGGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                                                                                   <  2:188200/101‑1 (MQ=21)
   cGTGGCGCTTTTGCGCAGGCAAAAGGGGGGGGCTGCCCCCCCCCTTGG.CTTCTGCCAATTCCCACCCCCCGGGATTTTGGGCAAGATGAAAAAACAAAggg                                                                                                  >  1:567806/1‑101 (MQ=255)
     tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                                               <  1:4109557/101‑1 (MQ=25)
       gcgcTTTTGCGCAGGCAAAAGGGGTGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                                                                             <  1:526971/101‑1 (MQ=21)
               gcgcAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                                     <  2:1669204/101‑1 (MQ=25)
                 cgacccgaaaAGGGGGCGCTG.CCCCCCGCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCaaa                                                                                  <  1:77379/94‑1 (MQ=17)
                  cAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaaa                                                                                  <  2:1112464/101‑1 (MQ=25)
                   ccccgaaaAGGGGGCTCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAata                                                                                <  1:1989155/96‑1 (MQ=17)
                   aGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAat                                                                                 <  1:4694603/101‑1 (MQ=21)
                    ggCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                                                <  2:3246101/101‑1 (MQ=25)
                    ggCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                                                <  2:2394440/101‑1 (MQ=25)
                      cgaaaAGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATcc                                                                             >  1:3545823/3‑101 (MQ=9)
                        aaaAGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                                           <  1:4557588/101‑1 (MQ=17)
                         aaagggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                                          >  2:490676/4‑101 (MQ=17)
                         aaagggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                                          >  2:2693804/4‑101 (MQ=17)
                          aagggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                                         >  2:187717/3‑101 (MQ=18)
                           ggggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                                         >  1:3464504/1‑101 (MQ=21)
                           agggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                                        >  2:2484948/2‑101 (MQ=18)
                            gggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                                       >  2:2963111/1‑101 (MQ=18)
                             ggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggggg                                                                       <  1:4196744/101‑1 (MQ=25)
                              gggCGCTG.CCCCCCTCTTGGCCTTCGGGCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                                                      <  1:3107933/101‑1 (MQ=25)
                              gggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGGTATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                                     >  1:2514684/1‑99 (MQ=17)
                              gggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                                     <  1:2542500/101‑3 (MQ=18)
                              gggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCCCGGCATTTTGAGCAAGATG.AAAGACCATGGGGTCATCTTTCCTCAAATATCCCcggggg                                                                      >  2:1127926/1‑101 (MQ=16)
                               ggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                                     <  2:4654299/101‑1 (MQ=25)
                                 cgcTG.CCCCCCTCTTGGCCTTCGGCCAATTCTCCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                                  <  2:2803561/101‑6 (MQ=21)
                                 cgcTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                                  <  1:1167898/101‑6 (MQ=21)
                                 cgcTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGt                                                                   <  1:2353004/101‑1 (MQ=25)
                                    tG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                                <  1:3350898/101‑1 (MQ=25)
                                    tG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                                <  1:607517/101‑1 (MQ=25)
                                    tG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                                >  1:3944849/1‑101 (MQ=25)
                                     g.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGGATTTTGAGCAAGATG.AAAGACCATGGGGTCATCTTGCCTCAAATATCCCGGGGGGGGTCCTg                                                               >  1:2907301/1‑101 (MQ=25)
                                        cccccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                             <  2:3327975/101‑1 (MQ=37)
                                         ccccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                                            <  2:430373/101‑1 (MQ=37)
                                         ccccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                                            >  1:3286893/1‑101 (MQ=37)
                                          cccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                           <  2:2289244/101‑1 (MQ=37)
                                            ctctTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                                         >  1:1723413/1‑101 (MQ=37)
                                            ctctTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                                         >  2:3482552/1‑101 (MQ=37)
                                             tctTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                                        <  1:1459164/101‑1 (MQ=37)
                                              ctTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACg                                                       >  1:4085158/1‑101 (MQ=25)
                                              ctTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACg                                                       <  1:1567318/101‑1 (MQ=37)
                                                tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                                     >  2:4381375/5‑101 (MQ=37)
                                                tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                                     >  1:726826/5‑101 (MQ=37)
                                                    cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                                 >  2:2452919/1‑101 (MQ=37)
                                                       cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                              >  1:2789889/1‑101 (MQ=37)
                                                       cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGAGGGCaga                                              >  2:4177219/1‑101 (MQ=37)
                                                         gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                            <  1:4382493/101‑1 (MQ=255)
                                                          ccAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                                           >  2:1781543/1‑101 (MQ=255)
                                                          ccAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                                           <  2:2352536/101‑1 (MQ=255)
                                                           cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                                          <  2:1048494/101‑1 (MQ=255)
                                                           cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGAGGGGGCCCTGAAAGGACGGGGGGCAGAGccc                                          >  2:629234/1‑101 (MQ=25)
                                                            aaTTCACCCCCCGAGGTATTTGGGGCAAGATG.AAAGACCGTGGGATCATCTTGCCTCAAATATCGCGCCGGGAGTCCTGAAAGGAGGGGGGGCACAGcgcc                                         >  2:3818712/1‑101 (MQ=19)
                                                            aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                         >  1:2444517/1‑101 (MQ=37)
                                                             aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                        >  2:1213101/1‑101 (MQ=37)
                                                             aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCACGGGGGCAGTCCTGATAGGACGGGGGACAGACccccc                                        >  2:1900101/1‑101 (MQ=25)
                                                              ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGTGGCAGAGcccccc                                       >  2:2976755/1‑101 (MQ=25)
                                                               tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCACAGCCCCCCt                                      >  2:459148/4‑101 (MQ=25)
                                                                caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                     >  2:203479/3‑101 (MQ=255)
                                                                 accccccGAGGGATTTGGAGCAAGATG.AAAGACCATGGGTGCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                    <  1:2169463/100‑1 (MQ=25)
                                                                   cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                                  >  2:2975898/1‑101 (MQ=255)
                                                                    ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                                 <  2:1454458/101‑1 (MQ=255)
                                                                    ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                                 >  1:4513128/1‑101 (MQ=255)
                                                                     cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg                                >  1:3533448/1‑101 (MQ=255)
                                                                       cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc                              >  1:4041019/1‑101 (MQ=255)
                                                                        gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                             >  1:137667/1‑101 (MQ=255)
                                                                         aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                            >  1:2350304/1‑101 (MQ=255)
                                                                           gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc                          >  2:3407244/1‑101 (MQ=255)
                                                                           gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc                          >  2:1123499/1‑101 (MQ=255)
                                                                            tATTTGGAGCAAGATG.AAAGTCCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                         <  1:380306/101‑1 (MQ=255)
                                                                            tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                         >  2:2754765/1‑101 (MQ=255)
                                                                            tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                         >  1:88993/1‑101 (MQ=255)
                                                                            tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                         >  1:3176031/1‑101 (MQ=255)
                                                                             aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                        >  2:705936/1‑101 (MQ=255)
                                                                             aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                        >  1:3761020/1‑101 (MQ=255)
                                                                              tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa                       <  2:3686629/101‑1 (MQ=255)
                                                                               ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                      >  2:809106/1‑101 (MQ=255)
                                                                                 ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc                    <  1:1041480/101‑1 (MQ=255)
                                                                                 ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc                    >  1:694005/1‑101 (MQ=255)
                                                                                   agcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGt                  >  1:1831823/3‑101 (MQ=255)
                                                                                    gcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGtt                 <  2:3548818/100‑1 (MQ=255)
                                                                                     cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc                >  1:1841831/1‑101 (MQ=255)
                                                                                     cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc                >  2:2001548/1‑101 (MQ=255)
                                                                                      aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc               >  2:2380499/1‑101 (MQ=255)
                                                                                       aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg              >  1:1029815/1‑101 (MQ=255)
                                                                                          gtgaaaGACCATGGGTTCATCTTGCCGCAAAGATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc            <  2:1289827/98‑1 (MQ=255)
                                                                                          atgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc            >  1:4635115/4‑101 (MQ=255)
                                                                                           tgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCt           >  2:3048687/3‑101 (MQ=255)
                                                                                            gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTc          <  2:2075615/100‑1 (MQ=255)
                                                                                            gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTc          >  2:3882507/2‑101 (MQ=255)
                                                                                            gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTc          >  1:389622/2‑101 (MQ=255)
                                                                                            gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTc          <  1:1077600/100‑1 (MQ=255)
                                                                                            gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGAAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGGTCCGACTc          >  2:2522902/2‑101 (MQ=255)
                                                                                             aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCCGCTcg         >  2:4252361/1‑100 (MQ=255)
                                                                                              aaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAg        <  2:1693988/101‑1 (MQ=255)
                                                                                              aaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGCCTTTCTCAAGCGTTCCGGCTCAg        >  1:1293108/1‑101 (MQ=255)
                                                                                                gACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGTGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAAaa      <  1:2976943/101‑1 (MQ=255)
                                                                                                gACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGaa      >  2:737620/1‑101 (MQ=255)
                                                                                                gACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGaa      >  2:2600141/1‑101 (MQ=255)
                                                                                                 aCCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAc     <  2:3286691/101‑1 (MQ=255)
                                                                                                    aTGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAAGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCgg  >  2:4102579/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
AACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGG  >  NC_014034/2640111‑2640309

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG