Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,731,800 G→C 100% P509P (CCC→CCG RCAP_rcc02540 ← diguanylate cyclase/phosphodiesterase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,731,8000G→C100.0% 629.4 169P509P (CCC→CCGRCAP_rcc02540diguanylate cyclase/phosphodiesterase
Reads supporting (aligned to +/- strand):  new base (105/62):  ref base (0/0):  total (107/62)

Only 100 of 176 total aligned reads displayed.

CAGATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCGGGGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCATGGCTGGC  >  NC_014034/2731705‑2731895
                                                                                               |                                                                                               
cAGATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcg                                                                                            >  1:1288169/1‑101 (MQ=255)
  gATCACCCGGACGCCCAGCTGCGGGCCGAAACGGTACTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATTGCCCGCCGCgcgcg                                                                                          >  2:3155025/1‑101 (MQ=255)
     cgcccGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcgctcgc                                                                                       <  2:4022960/99‑1 (MQ=255)
      ccccGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcgcgcgcg                                                                                      <  2:3482186/100‑1 (MQ=255)
      aCCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcgcgcgcg                                                                                      <  1:2598012/101‑1 (MQ=255)
       cccGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcgcgcgcgc                                                                                     >  1:2572394/1‑101 (MQ=255)
       cccGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcgcgcgcgc                                                                                     >  2:689764/1‑101 (MQ=255)
         cGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAg                                                                                   <  1:279481/101‑1 (MQ=255)
         cGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAg                                                                                   <  1:4491486/101‑1 (MQ=255)
             cGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCCGCTgc                                                                               >  2:2929015/1‑101 (MQ=255)
             cGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTgc                                                                               >  2:1739314/1‑101 (MQ=255)
              gCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTgcg                                                                              >  1:1388192/1‑101 (MQ=255)
               cccAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTgcgc                                                                             >  1:1024873/1‑101 (MQ=255)
                ccAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCCGCTGCGcc                                                                            >  2:2518114/1‑101 (MQ=255)
                 cAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCg                                                                           >  1:2257257/1‑101 (MQ=255)
                  aGCTGCGGGCCAAAACGGCCCTTTATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCgg                                                                          <  1:2157647/101‑1 (MQ=255)
                   gCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCggg                                                                         <  1:3057055/101‑1 (MQ=255)
                    gtGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGc                                                                        <  2:3661013/100‑1 (MQ=255)
                      gCGGGGCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCg                                                                      <  2:2350530/101‑1 (MQ=255)
                      gCGGGCCGAACCGGTCCTTGAGCTGCGGGATCTGGTCGAGGGACAGGAAGACCGGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCTGGCCg                                                                      <  1:289202/101‑1 (MQ=255)
                      gCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCg                                                                      >  2:2705639/1‑101 (MQ=255)
                       cGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGa                                                                     <  2:519329/101‑1 (MQ=255)
                       cGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGa                                                                     >  2:2305537/1‑101 (MQ=255)
                          gCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCt                                                                  <  1:608575/101‑1 (MQ=255)
                            cGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTgg                                                                <  1:4340719/101‑1 (MQ=255)
                            cGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTgg                                                                >  2:2675342/1‑101 (MQ=255)
                             gAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTggg                                                               >  1:4443528/1‑101 (MQ=255)
                             gAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTggg                                                               <  2:4742287/101‑1 (MQ=255)
                              aaaCGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGgc                                                              >  1:3571252/1‑101 (MQ=255)
                               aaCGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGgcg                                                             >  1:3071718/1‑101 (MQ=255)
                               aaCGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGgcg                                                             >  1:178852/1‑101 (MQ=255)
                                aCGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGgcgc                                                            >  2:207673/1‑101 (MQ=255)
                                 cGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGcgcg                                                           <  1:4262176/101‑1 (MQ=255)
                                   gTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGa                                                         >  2:1788546/1‑101 (MQ=255)
                                      cTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCCGCTGCGCCGGGCCGACCTGGGCGCGGAAgg                                                      <  1:4222977/101‑1 (MQ=255)
                                      cTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGCCCGACCTGGGCGCGGAAgg                                                      >  2:229236/1‑101 (MQ=255)
                                      cTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGAGCCGGGCCGACCTGGGCGCGGAAgg                                                      >  2:2138676/1‑101 (MQ=255)
                                       ttGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGa                                                     <  1:207698/101‑1 (MQ=255)
                                       ttGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGa                                                     >  2:4589857/1‑101 (MQ=255)
                                        tGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGAt                                                    >  1:1595090/1‑101 (MQ=255)
                                        tGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGAt                                                    >  1:558195/1‑101 (MQ=255)
                                        tGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGAt                                                    <  1:4655273/101‑1 (MQ=255)
                                         gATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGAtt                                                   <  1:2039167/101‑1 (MQ=255)
                                          aTCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTc                                                  <  1:3405797/101‑1 (MQ=255)
                                            ctgctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTcgc                                                >  1:2360953/1‑101 (MQ=255)
                                               ctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGAt                                             >  2:3573615/1‑101 (MQ=255)
                                               ctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGAt                                             >  2:1154274/1‑101 (MQ=255)
                                               ctcgAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCAGCGCGCGCGCGCAGATGCGCCGGGCCGACCTGGGCGCGGAAGGATTcgcgct                                             >  2:3459816/4‑99 (MQ=255)
                                                tgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGAtt                                            >  2:731679/1‑101 (MQ=255)
                                                tgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGAtt                                            >  2:806991/1‑101 (MQ=255)
                                                  gAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTgg                                          <  1:2486728/101‑1 (MQ=255)
                                                  gAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTgg                                          >  2:3733677/1‑101 (MQ=255)
                                                   aaCTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTggg                                         >  2:4129317/1‑101 (MQ=255)
                                                    aCTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGc                                        <  2:2511385/101‑1 (MQ=255)
                                                    aCTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGc                                        <  2:3907421/101‑1 (MQ=255)
                                                     cTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATGGGGcc                                       >  1:3839034/1‑100 (MQ=255)
                                                      gggTCGATCGACAGGAAGCCCTGCTGCAAGAGCCCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAg                                      <  2:1979146/100‑1 (MQ=255)
                                                       ggTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGc                                     >  1:2846028/1‑101 (MQ=255)
                                                       ggTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGc                                     >  2:857939/1‑101 (MQ=255)
                                                       ggTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGACGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGc                                     >  1:1028609/1‑101 (MQ=255)
                                                       ggTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGC..CGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGacc                                   >  2:1119922/1‑101 (MQ=255)
                                                        gTCGATCGACAGGAGGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGcc                                    <  2:2454192/101‑1 (MQ=255)
                                                        gTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGcc                                    >  2:1207327/1‑101 (MQ=255)
                                                         tcgatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGTAAGGATTCGCGATTGGGCAGccc                                   >  1:3675595/1‑101 (MQ=255)
                                                         tcgatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGccc                                   <  2:558144/101‑1 (MQ=255)
                                                            atcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGt                                >  2:821536/1‑101 (MQ=255)
                                                             tcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTc                               <  2:3816404/101‑1 (MQ=255)
                                                             tcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTc                               >  2:4625555/1‑101 (MQ=255)
                                                             acgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTc                               <  2:4420440/100‑1 (MQ=255)
                                                              cgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCa                              >  1:1195201/1‑101 (MQ=255)
                                                              cgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCa                              >  1:3187222/1‑101 (MQ=255)
                                                               gaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAg                             >  1:3729295/1‑101 (MQ=255)
                                                               gaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAg                             >  1:2106462/1‑101 (MQ=255)
                                                               gaCAGGAAAACCTGCTGCAAAAGATGGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAg                             <  1:719720/101‑1 (MQ=255)
                                                                agaGGAGGCCCTGCTGCAAGGGGCCCCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAgc                            <  2:3616725/99‑1 (MQ=255)
                                                                aCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAgc                            >  1:4398906/1‑101 (MQ=255)
                                                                   ggAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGca                         >  2:922039/1‑101 (MQ=255)
                                                                     aaGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGNAGCCGGGTCAGCGcatc                       >  2:1586053/1‑101 (MQ=255)
                                                                     aaGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGcatc                       >  2:95690/1‑101 (MQ=255)
                                                                     aaGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCAGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGCCAGCGcatc                       >  2:1895900/1‑101 (MQ=255)
                                                                       gACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGcatcat                     <  1:4590913/101‑1 (MQ=255)
                                                                         ccTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGc                   <  1:2246141/101‑1 (MQ=255)
                                                                           tgctgcAAGAGATCGCCCGCCGCGCGGGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGt                 >  2:4707802/1‑101 (MQ=255)
                                                                           tgctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGt                 >  2:3085437/1‑101 (MQ=255)
                                                                            gctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTc                >  1:3049153/1‑101 (MQ=255)
                                                                             ctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcg               >  1:1290783/1‑101 (MQ=255)
                                                                              tgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcgc              <  1:935949/101‑1 (MQ=255)
                                                                                cAAGAGATCGCCCGCCGCGCGCGCGCCCAGGTGCGCCGGGCCGGCCTGGGCGCGGCAGGATTCGCGGTTGGGCAGCCCGGGCAGCGCATCACGCGCcggct            >  1:3611869/1‑98 (MQ=255)
                                                                                 aaGAGATCGCCCGCCGCGCGCGCGCGCAGGTGCGCCGGGCCGCCCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCCGCGCATCATGCGTCGCGGc           >  1:1337020/1‑101 (MQ=255)
                                                                                 aaGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGc           >  2:2873070/1‑101 (MQ=255)
                                                                                  agagATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCa          <  2:1967709/101‑1 (MQ=255)
                                                                                   gagaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAt         >  1:1688473/1‑101 (MQ=255)
                                                                                    agaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtg        >  2:1393185/1‑101 (MQ=255)
                                                                                     gaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtgg       >  2:3339550/1‑101 (MQ=255)
                                                                                      aTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggc      >  1:1729494/1‑101 (MQ=255)
                                                                                      aTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggc      >  2:3551125/1‑101 (MQ=255)
                                                                                        cGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggctg    <  1:819766/101‑1 (MQ=255)
                                                                                         tcccGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggctgg   <  1:731753/100‑1 (MQ=255)
                                                                                         gCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggctgg   <  1:708919/101‑1 (MQ=255)
                                                                                          cccGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggctggc  >  1:1091394/1‑101 (MQ=255)
                                                                                               |                                                                                               
CAGATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCGGGGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCATGGCTGGC  >  NC_014034/2731705‑2731895

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG