Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,854	C→G	100%	intergenic (‑186/+107)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,854	0	C→G	100.0%	80.3	27	intergenic (‑186/+107)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (7/20):  ref base (0/0):  total (7/20)

ATAGGAAACGCGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTT  >  NC_014034/2747760‑2747947                                                                                                  |                                                                                               aTAGGAACCGCGCCCCGCTTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCgaga                                                                                           <  2:4195622/101‑1 (MQ=25)  tAGGAACCGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGaa                                                                                          <  1:493177/101‑1 (MQ=37)  tAGGAACCGCGACCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGaa                                                                                          <  2:1951242/101‑1 (MQ=37)  tAGGAAACGCGACACGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGaa                                                                                          >  2:2155094/1‑101 (MQ=37)  tAGAAACCGCGCCCCGCTTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGaa                                                                                          <  1:4555752/101‑1 (MQ=25)  gaGGAAGCGCCCCCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGaa                                                                                          <  1:3838747/100‑1 (MQ=25)  agggAACCGCGCCCCGGGTGC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGaa                                                                                          <  2:3344831/99‑1 (MQ=25)   aGGAAACGCGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAg                                                                                         <  2:2369916/101‑1 (MQ=255)    ggAAACGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAgc                                                                                        <  2:3045285/101‑1 (MQ=37)        aCGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgccc                                                                                    <  1:2061877/101‑1 (MQ=25)        aCGCGACCCGGGTTG.CGCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgccc                                                                                    <  1:4445886/101‑1 (MQ=25)        aCGAGACCCGCGTTC.CGCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCTCCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgccc                                                                                    <  2:1317268/101‑1 (MQ=25)         cgcgCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgcccg                                                                                   <  1:2721320/101‑1 (MQ=25)         cgcgACCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgcccg                                                                                   <  2:2855843/101‑1 (MQ=25)           cgccccGGGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgcccgcc                                                                                 <  1:3931988/98‑1 (MQ=21)              ccccgtttccccccGCCCGCAGCGCCCGGTTGCGGCATGCCCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                              <  1:1071047/91‑1 (MQ=21)               ccgttttt.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.ggtc                                                                            <  2:2520257/96‑3 (MQ=25)                ccgcttccccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggc                                                                            <  2:3535408/93‑3 (MQ=21)                  tcttcgccccGCCCTAAGCGCCGGGTTGGGGCATGCCCGCTCCACCCCGCACATCAGGGGGCAGCGCCCGG.CCCGGCGGGCGAAAGGCGCCCggc.gggccg                                                                          <  2:192946/95‑5 (MQ=9)                  ggttccccccGCCGGCAGCGCCCGGTTGCGGCATGCCCGCTTCAGCCCGCACCACAGAGGGCAGCGCGCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                          <  2:837940/95‑5 (MQ=21)                  gcttccccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCCCCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                          <  1:131274/95‑5 (MQ=21)                  cgTTTCCCCCGCCCGCAGCGCCCGGTGGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                          <  1:1474179/101‑5 (MQ=21)                                                            cAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCCCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGCGGTCACAGGAc                                 >  1:2035637/1‑101 (MQ=17)                                                                            gggCACCGCCCGG.CCCGGCGGGCGAGACGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGc                 <  2:359905/101‑1 (MQ=17)                                                                              gcggcccccggcccc.GCGGCCGAGACGCCGCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCa               <  2:503121/95‑1 (MQ=18)                                                                                     ccgg.cccgGCGGGGGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGAt        <  2:2035516/101‑1 (MQ=18)                                                                                      cgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg       >  2:3401351/1‑101 (MQ=18)                                                                                      cgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg       >  1:2063779/1‑101 (MQ=18)                                                                                       gg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg      >  2:975865/1‑101 (MQ=18)                                                                                          cccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGcc    >  1:1009587/5‑101 (MQ=18)                                                                                           ccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCt   >  2:3416581/4‑101 (MQ=21)                                                                                           ccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAACACGATGGCCt   >  2:1058617/4‑101 (MQ=21)                                                                                            cggcgGGCGAGAAGCGCCCACCGGGCCCGGAGCTACCCGAGGGCCTTTGGGCCCGCGGGGGCCACAGGACCTCGGTGACGCATGGCCAAGGCGATGGCCtt  >  2:3020093/3‑101 (MQ=21)                                                                                                  |                                                                                               ATAGGAAACGCGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTT  >  NC_014034/2747760‑2747947 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 21 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG