Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,791,928 A→G 100% E319E (GAA→GAG)  accC → acetyl‑CoA carboxylase biotin carboxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,791,9280A→G100.0% 503.9 133E319E (GAA→GAG) accCacetyl‑CoA carboxylase biotin carboxylase
Reads supporting (aligned to +/- strand):  new base (12/120):  ref base (0/0):  total (12/121)

Only 100 of 214 total aligned reads displayed.

GAGATGAACACCCGCCTGCAGGTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAAAT.AGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGCCGAACTTCGCCCCCTGCCCCGGCA  >  NC_014034/2791830‑2792025
                                                                                                  |                                                                                                  
gagaTGAACCCCCGCCTGCAGGTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAt                                                                                                  <  1:1833770/101‑1 (MQ=255)
 tcttGAACCCCCGCCTGCAGGTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGACAtg                                                                                                 <  2:2414160/98‑2 (MQ=255)
  gaggAACACCCGCCTGCAGGTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAtgg                                                                                                <  2:1868055/98‑3 (MQ=255)
  gaTGAACCCCCGCCTGCAGGTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAtgg                                                                                                <  2:22802/101‑3 (MQ=255)
   aTGAACCCCCGCCTGCAGGTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGa                                                                                              <  2:1389713/101‑1 (MQ=255)
   aTGAACACCCGCCTGCAGGTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGCAGAGAA.AGa                                                                                              >  2:2093508/1‑101 (MQ=255)
    tGAACACCCGCCTGCAGGTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGGGGAGACAtgaga                                                                                              >  1:2414295/1‑97 (MQ=37)
      aaCACCCGCCTGCAGGTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCTGCGAACAGATCCGCGTCGCGGCGGGGGGAGAGAT.GGaaat                                                                                           >  2:2299427/1‑99 (MQ=37)
      aaCACCCGCCTGCAGGTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGGAGAGAT.GGAAtt                                                                                           >  2:364413/1‑101 (MQ=255)
       acacCCGCCTGCAGGTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGGGAAGAGAT.GGAAtta                                                                                          >  1:4533408/1‑100 (MQ=255)
          cccGCCTGCAGGTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACg                                                                                       <  1:3110079/101‑1 (MQ=255)
              ccTGCAGGTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAgg                                                                                   <  1:3872052/101‑1 (MQ=255)
               cTGCAGGTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGGGGAGAGAG.GGAATTCACGCAGGa                                                                                  >  2:584585/1‑101 (MQ=38)
                tGCAGGTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGGGAGGAGGC.GGAAATCACGGAGGaa                                                                                 >  1:1646796/1‑101 (MQ=37)
                 gCAGGTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGaaa                                                                                <  1:3026097/101‑1 (MQ=255)
                 gCAGGTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGaaa                                                                                <  2:1781165/101‑1 (MQ=255)
                   aGGTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGGGAAGAGAA.GGAATTCACGCCGGaaaaa                                                                              >  2:299929/1‑100 (MQ=255)
                    ggTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAAcc                                                                             <  1:2441824/101‑1 (MQ=255)
                     gTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGGATTTACCCCGGAAAAACt                                                                            >  1:2283850/1‑101 (MQ=255)
                       ggAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGc                                                                          <  2:3094905/101‑1 (MQ=255)
                       ggAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGc                                                                          <  2:4705037/101‑1 (MQ=255)
                       ggAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGc                                                                          <  1:1924031/101‑1 (MQ=255)
                         aaCATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAg                                                                        <  2:1810736/101‑1 (MQ=255)
                          aCATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGGGGAGAGAT.GGAATTCACGCAGGGAAACACGCAGa                                                                       >  1:3488581/1‑101 (MQ=255)
                           cATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGGGGAGAGATGGGAATTAACGCGGAaaaaacgcagca                                                                       >  2:3731356/1‑93 (MQ=255)
                           cATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGAt                                                                      <  2:491041/101‑1 (MQ=255)
                            aTCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGCAGGGAT.GGGATTTAAGCAGGAAAACCTGCAGATc                                                                     >  2:3310141/1‑101 (MQ=255)
                            aTCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATc                                                                     <  1:3236339/101‑1 (MQ=255)
                              ccGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCcg                                                                   <  1:369576/101‑1 (MQ=255)
                              ccGGTGACCGAGGACATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGGGGAGAGAT.GGAATTCACGCAGGAAAACCTGCAGAAccc                                                                   >  2:2742120/1‑100 (MQ=255)
                               cGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGGGGAGAGAT.GGAATTCACGCAGGAAAAAATGCAAATCcgc                                                                  >  1:3996098/1‑101 (MQ=255)
                               cGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGGGGAGAGAA.GGAAGTTACGCAGGCAAACCTGCAAATCcgc                                                                  >  2:1298807/1‑101 (MQ=255)
                                 gTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGGGGAGAGAT.GGAATTTACGCAGGGAAACCCGCAGATCCCCgg                                                                >  2:1538737/1‑101 (MQ=255)
                                  tGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGc                                                               <  2:1619639/101‑1 (MQ=255)
                                   gACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGGGGAGAGAT.GGGATTTACCCAGGAAAAACCGCAGAACCCCCGcc                                                              >  1:48770/1‑101 (MQ=255)
                                   gACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGGAGAGAT.GGAATTTACGCAGGAAAAACTCCAGATCCGCGGcc                                                              >  2:3787233/1‑101 (MQ=255)
                                   gACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGGAGAGAA.GGAAATTACCCAGGAAAACCTGCAGAACCGCGGcc                                                              >  1:2896219/1‑101 (MQ=255)
                                   gACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGcc                                                              <  1:4392671/101‑1 (MQ=255)
                                   gACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGcc                                                              <  2:614350/101‑1 (MQ=255)
                                    aCCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCa                                                             <  2:3920818/101‑1 (MQ=255)
                                     ccGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCAt                                                            <  1:957418/101‑1 (MQ=255)
                                      cGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATg                                                           <  1:2505001/101‑1 (MQ=255)
                                      cGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAA.GGAATTTACGCAGGAAAAACTGCAGATACGCGGCCCTg                                                           >  2:3605991/1‑101 (MQ=255)
                                       gAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGGGGAGAGAT.GGAATTCACGCAGGAAAAACTGCAGATCCGCGGCCACGc                                                          >  1:2337360/1‑101 (MQ=255)
                                       gAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGGAGAGAT.GGAATTTACGCAGGAAAACCTGCAGATACGCGGCCATGc                                                          >  1:1094073/1‑101 (MQ=255)
                                       gAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGc                                                          <  1:2525426/101‑1 (MQ=255)
                                       gAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGc                                                          <  2:3993220/101‑1 (MQ=255)
                                       gAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGc                                                          <  1:1017477/101‑1 (MQ=255)
                                        aGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGGAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCCTGCg                                                         >  2:3631189/1‑101 (MQ=255)
                                         gggCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGa                                                        <  1:15524/101‑1 (MQ=255)
                                           gCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATc                                                      <  1:364447/101‑1 (MQ=255)
                                           gCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATc                                                      <  1:4674499/101‑1 (MQ=255)
                                              tCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAg                                                   <  1:1244313/101‑1 (MQ=255)
                                               cTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGGAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGCgg                                                  >  1:1754588/1‑101 (MQ=255)
                                                 ttccgatctaTCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTg                                                <  1:363558/92‑1 (MQ=255)
                                                   ggCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCg                                              <  1:3884932/101‑1 (MQ=255)
                                                   ggCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCg                                              <  1:456120/101‑1 (MQ=255)
                                                      gTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGGGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGGGCgggt                                           >  1:622640/1‑99 (MQ=255)
                                                      gTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGGAGAGAT.GGAATTCACGCAGGAAAAACTGCAGATCCGCGGCCCTGCGCTCGAGGGGGGGAt                                           >  1:380012/1‑101 (MQ=255)
                                                       tCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATc                                          <  1:1444785/101‑1 (MQ=255)
                                                       tCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATc                                          <  1:2760606/101‑1 (MQ=255)
                                                       tCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATc                                          <  2:170039/101‑1 (MQ=255)
                                                        cGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCa                                         <  2:1426011/101‑1 (MQ=255)
                                                         gATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGGAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGGGCGGATCaa                                        >  1:3528481/1‑101 (MQ=255)
                                                         gATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCaa                                        <  2:3161527/101‑1 (MQ=255)
                                                           tctcGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATg                                      <  1:1382029/101‑1 (MQ=255)
                                                           tctcGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATg                                      <  1:1946604/101‑1 (MQ=255)
                                                              cGTCCGCGAACAGATCCGCGTCGCGGCGGGCGGAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGGGGGGATCAATGCCg                                   >  1:2233509/1‑101 (MQ=255)
                                                              cGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAAATCACGCAGGAAAAACTGCAGATCCGCGGCCATGCGATCGAGGGGCGGGTCAAAGCCg                                   >  1:4309245/1‑101 (MQ=255)
                                                               gTCCGCGAACAGATCCGCGTCGCGGCGGGGGCAGAGAA.GGAATTTACGCAGGAAAACCTGCAGATCCGCGGGCATGCGATCGAGGGGCGGAGCAAAGTCGa                                  >  1:4241889/1‑101 (MQ=255)
                                                               gTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGGGCGGGTCAATGCCGa                                  >  2:2527799/1‑101 (MQ=255)
                                                                tCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGaa                                 <  1:2093629/101‑1 (MQ=255)
                                                                 ccGCGAACAGATCCGCGTCGCGGCGGGCGGAGAGAT.GGGATTCACGCCGGAAAAACCGCAGATCCGCGGCCCTGCGACCGAGGGGCGGGTCAAAGCCgcaa                                >  2:785779/1‑98 (MQ=255)
                                                                   gcgAACAGATCCGCGTCGCGGCGGGGGAAGAGAT.GGAAATTACGCAGGGAAACCTGCAGATCCGCGGCCATGGGAGCGAGGGGCGGATCAAAGCCGAACgg                              >  1:2916488/1‑101 (MQ=255)
                                                                    cgAACAGATCCGCGTCGCGGCGGGCGAAGAAAA.GGAATTTACGGAGGGAAACCTGCAGAACCGCGGCCATGCGGTCCAGGTGCGGGGCAATGCCGAACGGc                             >  1:4133387/1‑101 (MQ=255)
                                                                     gAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTTACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGGGGGGATCAATGCCGAAAGGcg                            >  2:280705/1‑100 (MQ=255)
                                                                     gAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCt                            <  1:719053/101‑1 (MQ=255)
                                                                     gAACAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGGGGGGATCAAAGCCGAACGGcg                            >  2:2321046/1‑100 (MQ=255)
                                                                      aaCAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTg                           <  1:4161493/101‑1 (MQ=255)
                                                                        cAGATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCAGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGcc                         <  2:104342/101‑1 (MQ=255)
                                                                          gATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGGGCGGATCAATGCCGAACGGCCGCCGa                       >  2:2762317/1‑101 (MQ=255)
                                                                          gATCCGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCGGCAGATCCGCGGGCATGCGATCGAGGGGCGGGTCAAAGCCGAACGGCTGGCGa                       >  2:4106614/1‑101 (MQ=255)
                                                                             ccGCGTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGCCGAACt                    <  1:3084880/101‑1 (MQ=255)
                                                                              cgcgTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGCCGCACtt                   >  2:4393309/1‑101 (MQ=255)
                                                                              cgcgTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGCCGAACtt                   <  1:3731586/101‑1 (MQ=255)
                                                                               gcgTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGCCGAACTTc                  <  2:442273/101‑1 (MQ=255)
                                                                                cgTCGCGGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAAACTGCAGATACGCGGCCATGCGATCGAGGGGCGGATCAATGCCGAACGGCTGCCGCACtttc                 >  2:459599/1‑99 (MQ=255)
                                                                                   cgcgGCGGGCGAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGCCGAACTTCGccc              <  2:2896043/101‑1 (MQ=255)
                                                                                      ggcgggcgAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGCCGAACTTCGCCCCCt           <  1:3424124/101‑1 (MQ=255)
                                                                                      ggcgggcgAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGCCGAACTTCGCCCCCt           >  2:4329001/1‑101 (MQ=255)
                                                                                          ggcgAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGCCGAACTTCGCCCCCTGccc       <  2:3884239/101‑1 (MQ=255)
                                                                                           gcgAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGCCGAACTTCGCCCCCTGcccc      >  1:2558601/1‑101 (MQ=255)
                                                                                           gcgAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGCCGAACTTCGCCCCCTGcccc      >  2:1142671/1‑101 (MQ=255)
                                                                                           gcgAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGCCGAACTTCGCCCCCTGcccc      <  2:3380546/101‑1 (MQ=255)
                                                                                            ggaagagAT.GGAATTCACGCAGGAAACCCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGCCGAACTTCGCCCCCTGCCCCg     <  2:805293/100‑1 (MQ=255)
                                                                                            cgAAGAGAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGCCGAACTTCGCCCCCTGCCCCg     <  1:4725923/101‑1 (MQ=255)
                                                                                             gaagagAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGCCGAACTTCGCCCCCTGCCCCgg    >  2:3525631/1‑101 (MQ=255)
                                                                                              aagagAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGCCGAACTTCGCCCCCTGCCCCGGc   >  1:1526779/1‑101 (MQ=255)
                                                                                              aagagAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGCCGAACTTCGCCCCCTGCCCCGGc   >  1:491095/1‑101 (MQ=255)
                                                                                               agagAT.GGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGCCGAACTTCGCCCCCTGCCCCGGCa  <  2:1742579/101‑1 (MQ=255)
                                                                                                  |                                                                                                  
GAGATGAACACCCGCCTGCAGGTGGAACATCCGGTGACCGAGGGCATCTTCGGCGTCGATCTCGTCCGCGAACAGATCCGCGTCGCGGCGGGCGAAGAAAT.AGAATTCACGCAGGAAAACCTGCAGATCCGCGGCCATGCGATCGAGGTGCGGATCAATGCCGAACGGCTGCCGAACTTCGCCCCCTGCCCCGGCA  >  NC_014034/2791830‑2792025

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG