Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,808,441 C→T 100% P352L (CCC→CTC)  pyrD2 → dihydroorotate oxidase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,808,4410C→T100.0% 810.9 215P352L (CCC→CTC) pyrD2dihydroorotate oxidase
Reads supporting (aligned to +/- strand):  new base (80/135):  ref base (0/0):  total (80/135)

Only 100 of 222 total aligned reads displayed.

TCTGGCGGCCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCCCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCG  >  NC_014034/2808343‑2808538
                                                                                                  |                                                                                                 
tCTGGCGGCCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCt                                                                                                 >  1:1338941/1‑101 (MQ=255)
tCTGGCGGCCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCt                                                                                                 >  1:1961945/1‑101 (MQ=255)
tCTGGCGGCCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCt                                                                                                 >  2:3817352/1‑101 (MQ=255)
tCTGGCGGCCGAGGTCGCGCGCGGCCTGGATGCCCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCt                                                                                                 <  1:4044058/101‑1 (MQ=255)
 cTGGCGGCCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTg                                                                                                >  2:2355183/1‑101 (MQ=255)
 cTGGCGGCCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTg                                                                                                <  2:4077417/101‑1 (MQ=255)
 cTGGCGGCCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGCAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTg                                                                                                <  1:4723723/101‑1 (MQ=255)
  tGGCGGCCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGa                                                                                               >  2:2466835/1‑101 (MQ=255)
   ggcggcCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAc                                                                                              >  1:1499627/1‑101 (MQ=255)
   ggcggcCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAc                                                                                              >  1:3682907/1‑101 (MQ=255)
   ggcggcCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAc                                                                                              <  1:3094355/101‑1 (MQ=255)
        ccGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAcccgcc                                                                                         >  2:2063568/1‑101 (MQ=255)
        ccGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAcccgcc                                                                                         <  2:3220682/101‑1 (MQ=255)
          gAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAcccgcccg                                                                                       >  2:78321/1‑101 (MQ=255)
           gggTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGt                                                                                      <  2:98846/100‑1 (MQ=255)
           aGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGt                                                                                      <  2:2007078/101‑1 (MQ=255)
             gTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTCCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAg                                                                                    <  2:627324/101‑1 (MQ=255)
                 cgcgcgGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGc                                                                                <  1:629941/101‑1 (MQ=255)
                 cgcgcgGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGc                                                                                <  1:536029/101‑1 (MQ=255)
                   cgcgGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCgg                                                                              <  1:249340/101‑1 (MQ=255)
                    gcgGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCggg                                                                             >  1:3027049/1‑101 (MQ=255)
                     cgGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCgggg                                                                            >  2:1900577/1‑101 (MQ=255)
                      ggCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCggggg                                                                           >  1:98646/1‑101 (MQ=255)
                        ccTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGGGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGtt                                                                         <  1:1417697/101‑1 (MQ=255)
                        ccTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGtt                                                                         <  1:2792673/101‑1 (MQ=255)
                          gggATGCGCTTTTGGCGCGCGACGGTTTTGCTTGGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTc                                                                       <  2:3443065/100‑1 (MQ=255)
                            gATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTcac                                                                     <  1:2466978/101‑1 (MQ=255)
                             aTGCGTTTTTGGCGCGCGCCTGCTTGGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTcaca                                                                    <  2:1386969/101‑1 (MQ=38)
                             aTGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTcaca                                                                    <  2:3270794/101‑1 (MQ=255)
                                  cTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcaccccc                                                               <  1:183775/101‑1 (MQ=255)
                                  cTTGTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcaccccc                                                               <  2:3148573/101‑1 (MQ=255)
                                   ttttGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcacccccg                                                              <  1:4628400/101‑1 (MQ=255)
                                   ttttGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcacccccg                                                              >  1:4427061/1‑101 (MQ=255)
                                    tttGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcacccccgc                                                             <  2:3586728/101‑1 (MQ=255)
                                     ttGGCGCGCGCCGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcacccccgca                                                            <  1:4573538/101‑1 (MQ=255)
                                     ttGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcacccccgca                                                            >  2:4139903/1‑101 (MQ=255)
                                       ggCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcacccccgcacc                                                          <  2:4313002/101‑1 (MQ=255)
                                        gcgcgcGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcacccccgcaccc                                                         <  1:1537392/101‑1 (MQ=255)
                                        gcgcgcGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcacccccgcaccc                                                         <  2:277647/101‑1 (MQ=255)
                                        gcgcgcGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGGTTCAcacccccgcaccc                                                         >  1:1789659/1‑101 (MQ=255)
                                          gcgggACGGTTTTGCTTGGGTCGCGGAAGCCGTGGGCTCCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcacccccgcaccccc                                                       <  1:3301657/101‑1 (MQ=37)
                                          gcgcgTCGGCTTTGTTGCGGCGGTGGAAGCCGTCGACACCGGTCGCAGCCGCTGGGTCTGGCCCGCCCTTAGCTGCGGGGGTTTCAcgcccccgcaccccc                                                       <  2:1868822/101‑1 (MQ=255)
                                           cgcgCCGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCAcacccccgcacccccg                                                      <  2:2137021/101‑1 (MQ=255)
                                           cgcgACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGATGCGGGGGGTTCAcacccccgcacccccg                                                      >  2:2744901/1‑101 (MQ=255)
                                            gcgACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGGTCCCcacccccgcccccccgc                                                     >  2:2299391/1‑100 (MQ=255)
                                              gACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTgg                                                   <  2:1913887/101‑1 (MQ=255)
                                                   ttttGCTGCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTAt                                              <  2:2803487/100‑1 (MQ=255)
                                                   cTTTGCTCCGGTCGCGGAAGCCGTGGGCCCCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTAt                                              <  1:4452275/101‑1 (MQ=255)
                                                    tttGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTAtt                                             <  1:1645800/101‑1 (MQ=255)
                                                    tttGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTAtt                                             <  1:2952079/101‑1 (MQ=255)
                                                    tttGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCCGGGGGGTCTcaccccccccccccccccgcgccta                                             >  1:2803652/1‑91 (MQ=255)
                                                    ttggCTTGGGTCGCGAAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTAtt                                             <  1:843897/98‑1 (MQ=25)
                                                     ttGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTAttt                                            <  1:4291922/101‑1 (MQ=255)
                                                     tggCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTAttt                                            <  2:1501066/99‑1 (MQ=255)
                                                      tGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTg                                           <  2:4044536/101‑1 (MQ=255)
                                                      tGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGGTTCACACCCCCGCACCCCCCCGGCGTATTTg                                           >  2:2237265/1‑101 (MQ=37)
                                                          tCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGcc                                       <  2:1696197/101‑1 (MQ=255)
                                                           cGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCa                                      <  1:1625256/101‑1 (MQ=255)
                                                             gTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAg                                    <  1:78328/101‑1 (MQ=255)
                                                             gTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAg                                    <  1:3978217/101‑1 (MQ=255)
                                                             gTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAg                                    <  1:2119323/101‑1 (MQ=255)
                                                              gggcgAAAGCCATGGCCACCGGTCGCAGCCGCTGGCTATGACCCGCCCGTAGCTGCGGGGGTTTCACTCCCCCGCACCCCCGTGGCTTATTTGCGCCAAGa                                   <  2:1094553/99‑1 (MQ=18)
                                                               cgcgGAAGCCGTGGGCCCCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGaa                                  <  2:2959121/101‑1 (MQ=255)
                                                               cgcgGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGaa                                  <  1:3571949/101‑1 (MQ=255)
                                                               cgcgGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGaa                                  <  2:1436202/101‑1 (MQ=255)
                                                                 cgGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGGTTCACACCCCCCCACCCCCCCGGCGGGTTTTGGCCCAGaaaa                                >  1:4187238/1‑101 (MQ=255)
                                                                  ggAATCTGTGGGCACTGGTCTCTGCCTCTGGCTTTTTCCCTCCCGTAGCTGCGGGGGTTTCTCTCCCCCTCTCCCCCGTGGCTTATTTGCGCCAAGaaaaa                               <  2:1175662/101‑1 (MQ=255)
                                                                  ggAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGaaaaa                               >  2:1558017/1‑101 (MQ=255)
                                                                    aaGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGc                             >  1:1182566/1‑101 (MQ=255)
                                                                    aaGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGc                             <  1:3457205/101‑1 (MQ=255)
                                                                       ccGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGccca                          <  1:2714822/101‑1 (MQ=255)
                                                                       ccGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGcaca                          <  1:2355316/101‑1 (MQ=255)
                                                                        cGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAg                         <  1:1052719/101‑1 (MQ=255)
                                                                        cGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAg                         <  2:1584791/101‑1 (MQ=255)
                                                                        cGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAg                         <  2:1338849/101‑1 (MQ=255)
                                                                           gggCCCCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACt                      <  2:4334286/101‑1 (MQ=255)
                                                                           gggCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGGTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACt                      >  1:3350815/1‑101 (MQ=255)
                                                                            ggCCCCGGGCGCAGCCGCTGGCTCTGACCCTCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACtt                     <  1:1278119/101‑1 (MQ=18)
                                                                             gCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACttt                    <  2:3682678/101‑1 (MQ=255)
                                                                             gCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACttt                    <  2:1644586/101‑1 (MQ=255)
                                                                              cACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACtttt                   <  2:4550273/101‑1 (MQ=255)
                                                                               aCCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTg                  <  2:4225349/101‑1 (MQ=255)
                                                                               aCCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTg                  <  2:392730/101‑1 (MQ=255)
                                                                                ccGGTCGCAGCCGCTGGCTCTGCCCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGc                 <  2:1302054/101‑1 (MQ=255)
                                                                                ccGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGc                 <  2:124906/101‑1 (MQ=255)
                                                                                ccGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGc                 <  1:2237389/101‑1 (MQ=255)
                                                                                    tCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGt             <  2:388804/101‑1 (MQ=255)
                                                                                    tCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGt             <  2:723931/101‑1 (MQ=255)
                                                                                     cGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGtt            <  2:2598392/101‑1 (MQ=255)
                                                                                      gCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTc           <  1:2922070/101‑1 (MQ=255)
                                                                                      gCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTc           <  1:3873776/101‑1 (MQ=255)
                                                                                       cAGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCa          <  2:3026861/101‑1 (MQ=255)
                                                                                        aGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCAc         <  1:3121637/101‑1 (MQ=255)
                                                                                        aGCCGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCAAAGACTTTTGCCCGTTCAc         >  1:4341338/1‑101 (MQ=255)
                                                                                           cGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCt      >  1:165434/1‑101 (MQ=255)
                                                                                           cGCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCt      <  1:378008/101‑1 (MQ=255)
                                                                                            gCTGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTg     <  2:4186992/101‑1 (MQ=255)
                                                                                              tGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCCCACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGCCCTTTGCCCGTTCACCCTCgc   >  2:1209557/1‑101 (MQ=14)
                                                                                              tGGCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGgc   >  1:3963506/1‑101 (MQ=255)
                                                                                               ggCTCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGgcg  >  2:4743302/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
TCTGGCGGCCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCCCTGACCCGCCCGTAGCTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCG  >  NC_014034/2808343‑2808538

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG