Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,809,617 G→T 100% P318H (CCC→CAC)  acsA2 ← acetate‑‑CoA ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,809,6170G→T100.0% 581.7 178P318H (CCC→CAC) acsA2acetate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  new base (90/88):  ref base (0/0):  total (90/88)

Only 100 of 180 total aligned reads displayed.

ACACCGCCCAGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGGGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTATTGGTGCGTCATCGCGGCATA  >  NC_014034/2809519‑2809705
                                                                                                  |                                                                                        
acacCGCCCAGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGa                                                                                        <  1:2022368/101‑1 (MQ=255)
acacCGCCCAGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGa                                                                                        >  1:4139835/1‑101 (MQ=255)
 cacCGCCCAGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTGGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGAc                                                                                       >  2:3339418/1‑101 (MQ=255)
 cacCGCCCAGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGAc                                                                                       <  2:2393831/101‑1 (MQ=255)
 cacCGCCCAGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGAc                                                                                       <  2:2312750/101‑1 (MQ=255)
  cccGCCCAGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGAcc                                                                                      <  2:4539832/100‑1 (MQ=255)
   ccGCCCAGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGAccc                                                                                     >  2:2989039/1‑101 (MQ=255)
     gCCCAGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGt                                                                                   >  1:3381432/1‑101 (MQ=255)
       ccAGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCa                                                                                 >  1:1387928/1‑101 (MQ=255)
        cAGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCAc                                                                                >  2:2753442/1‑101 (MQ=255)
         aGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCAcc                                                                               >  1:242765/1‑101 (MQ=255)
          gAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCAccc                                                                              >  2:2562266/1‑101 (MQ=255)
          gAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCAccc                                                                              >  2:2186587/1‑101 (MQ=255)
           aaCCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCAccac                                                                             >  2:4664149/1‑99 (MQ=255)
           aaCCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCa                                                                             <  1:191779/101‑1 (MQ=255)
             ccGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGc                                                                           <  1:3676805/101‑1 (MQ=255)
             ccGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGc                                                                           <  1:3268894/101‑1 (MQ=255)
             ccGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGAAGCTGTGACCCGTCACCCAGc                                                                           >  1:2123486/1‑101 (MQ=255)
              cGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGcc                                                                          <  1:4241132/101‑1 (MQ=255)
              cGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGcc                                                                          <  2:3628698/101‑1 (MQ=255)
               gCCCCGCATCGGGCCCGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGccc                                                                         <  2:447793/101‑1 (MQ=255)
               gCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGccc                                                                         <  1:2478329/101‑1 (MQ=255)
                ccccGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCca                                                                        <  1:3292225/101‑1 (MQ=255)
                 cccGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCcac                                                                       <  2:3381226/101‑1 (MQ=255)
                 cccGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCcac                                                                       <  1:1000213/101‑1 (MQ=255)
                  ccGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCcaca                                                                      <  1:249443/101‑1 (MQ=255)
                   cGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACAt                                                                     <  1:913731/101‑1 (MQ=255)
                    gCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCTTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATc                                                                    >  1:3111981/1‑101 (MQ=255)
                    gCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATc                                                                    <  2:2536553/101‑1 (MQ=255)
                    gCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATc                                                                    >  2:1584167/1‑101 (MQ=255)
                    gCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATc                                                                    >  1:2240765/1‑101 (MQ=255)
                     cATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATcg                                                                   >  1:4176012/1‑101 (MQ=255)
                     cATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATcg                                                                   >  1:969376/1‑101 (MQ=255)
                        cGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATcggcg                                                                >  2:580526/1‑101 (MQ=255)
                         gggCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATCTAGCTGTGACCCGTCACCCAGCCCACATcggcgg                                                               >  1:4076910/1‑101 (MQ=255)
                          ggCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGt                                                              >  2:1744627/1‑101 (MQ=255)
                          ggCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGt                                                              >  2:1066951/1‑101 (MQ=255)
                           gCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCAGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTg                                                             <  1:1721837/101‑1 (MQ=255)
                            ccAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGc                                                            <  1:3398086/101‑1 (MQ=255)
                             cAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCa                                                           <  2:34115/101‑1 (MQ=255)
                             cAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCa                                                           >  1:1205312/1‑101 (MQ=255)
                              aGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCAc                                                          <  2:1561515/101‑1 (MQ=255)
                               ggTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGGGCCcc                                                         >  2:2337083/1‑101 (MQ=255)
                                gTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCa                                                        >  2:3284427/1‑101 (MQ=255)
                                gTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCa                                                        <  2:150391/101‑1 (MQ=255)
                                gTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCa                                                        <  1:4526133/101‑1 (MQ=255)
                                gTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCAGTGCACCa                                                        <  2:445593/101‑1 (MQ=255)
                                 tCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAg                                                       <  2:2684567/101‑1 (MQ=255)
                                   ggCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGaa                                                     <  2:307072/101‑1 (MQ=255)
                                   ggCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGaa                                                     >  1:1120261/1‑101 (MQ=255)
                                   ggCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGaa                                                     <  1:439114/101‑1 (MQ=255)
                                    gCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAac                                                    <  2:67359/101‑1 (MQ=255)
                                      aCGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAacac                                                  <  1:3427413/101‑1 (MQ=255)
                                      aCGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAacac                                                  >  2:791930/1‑101 (MQ=255)
                                        gCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATAATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGt                                                <  1:975419/101‑1 (MQ=255)
                                         cccTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGACGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTc                                               >  2:1859568/1‑101 (MQ=255)
                                         cccTCGAACATCAGCGTCGTCCCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTc                                               <  1:584186/101‑1 (MQ=255)
                                          ccTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCg                                              >  2:3819051/1‑101 (MQ=255)
                                            tCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGcc                                            <  1:4562094/101‑1 (MQ=255)
                                            tCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGcc                                            <  2:93285/101‑1 (MQ=255)
                                            tCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGAACCAGAACAGGTCGcc                                            >  1:4488129/1‑101 (MQ=255)
                                              gAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGt                                          >  2:3074612/1‑101 (MQ=255)
                                                  aTCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCtt                                      <  1:1067030/101‑1 (MQ=255)
                                                  aTCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCtt                                      <  1:2337216/101‑1 (MQ=255)
                                                   tCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTg                                     <  2:2872265/101‑1 (MQ=38)
                                                    cAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGt                                    <  1:4650693/101‑1 (MQ=37)
                                                     aGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTa                                   <  2:3885615/101‑1 (MQ=25)
                                                     aGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTa                                   >  2:4084065/1‑101 (MQ=37)
                                                      gCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTaa                                  <  1:716777/101‑1 (MQ=21)
                                                        gtcgtcGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGCCCTTGTAATc                                >  1:4728606/1‑101 (MQ=12)
                                                         tcgtcgCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCa                               <  1:2853739/101‑1 (MQ=17)
                                                         tcgtcgCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCa                               <  2:3992701/101‑1 (MQ=17)
                                                         gcgtcgCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCa                               <  2:2123365/100‑1 (MQ=17)
                                                          cgtcgCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCaa                              >  2:1943626/1‑101 (MQ=17)
                                                           gtcgCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCaaa                             >  1:1257370/1‑101 (MQ=18)
                                                           gtcgCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCaaa                             >  2:206183/1‑101 (MQ=18)
                                                           gtcgCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCaaa                             <  1:524693/101‑1 (MQ=17)
                                                            tcgCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAAc                            <  1:2657886/101‑1 (MQ=17)
                                                            tcgCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAAc                            <  1:2137732/101‑1 (MQ=17)
                                                            tcgCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAAc                            <  2:114572/101‑1 (MQ=17)
                                                             cgcgCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACg                           >  1:2132145/1‑101 (MQ=14)
                                                              gcgcCGTTCGTCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACgt                          <  2:1308023/101‑1 (MQ=12)
                                                               cgcCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACgtg                         <  2:1582103/101‑1 (MQ=17)
                                                                gcCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACgtgt                        >  2:1980877/1‑101 (MQ=17)
                                                                gcCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACgtgt                        <  1:757729/101‑1 (MQ=17)
                                                                gcCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACgtgt                        >  2:1395406/1‑101 (MQ=17)
                                                                 ccGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTa                       <  2:3514718/101‑1 (MQ=17)
                                                                 ccGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTa                       <  2:246440/101‑1 (MQ=17)
                                                                  cGTTGGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTAt                      >  2:1788963/1‑101 (MQ=9)
                                                                  cGTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTAt                      >  1:287852/1‑101 (MQ=17)
                                                                   gTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTAtt                     <  2:969299/101‑1 (MQ=11)
                                                                   gTTCGCCAAGGGCCCGTAGATGATGTAGCTGTGACCAGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTAtt                     >  2:4484125/1‑101 (MQ=2)
                                                                      cGCCAAGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTATTGGt                  >  2:585298/1‑101 (MQ=11)
                                                                        ccAAGGGCCCGTAGATGATGTCGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTATTGGTGc                >  1:2701449/1‑101 (MQ=2)
                                                                          aaGGGCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTATTGGTGCGt              >  2:3427877/1‑101 (MQ=11)
                                                                            gggCCCGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTATTGGTGCGTCa            >  1:447235/1‑101 (MQ=11)
                                                                                 cGTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTATTGGTGCGTCATcgcg       >  2:1207877/1‑101 (MQ=11)
                                                                                  gTAGATGATGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTATTGGTGCGTCATCGCgg      >  1:1457750/1‑101 (MQ=11)
                                                                                     gatgatGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTATTGGTGCGTCATCGCGGCAt   >  2:1453873/1‑101 (MQ=11)
                                                                                     gatgatGTAGCTGTGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTATTGGTGCGTCATCGCGGCAt   >  2:3255074/1‑101 (MQ=11)
                                                                                                  |                                                                                        
ACACCGCCCAGAACCGCCCCGCATCGGGCCAGGTCGGCACGCCCTCGAACATCAGCGTCGTCGCGCCGTTCGCCAAGGGCCCGTAGATGATGTAGCTGGGACCCGTCACCCAGCCCACATCGGCGGTGCACCAGAACACGTCGCCGTCCTTGTAATCAAACGTGTATTGGTGCGTCATCGCGGCATA  >  NC_014034/2809519‑2809705

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG