Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,819,668 G→C 15.9% A73G (GCG→GGG)  dacB ← penicillin‑binding protein 4

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,819,6680G→C15.9% 10.5 ‑1.7 ‑0.056A73G (GCG→GGG) dacBpenicillin‑binding protein 4
Reads supporting (aligned to +/- strand):  new base (0/9):  ref base (12/35):  total (12/44)
Fisher's exact test strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.93e-01

Only 100 of 117 total aligned reads displayed.

GACCGGCCCCGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCAGCCGCGCCGCCGCGATCAG  >  NC_014034/2819568‑2819768
                                                                                                      |                                                                                                     
gaCCGGCCCCGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAggcggg.cg                                                                                                       <  1:2313795/101‑1 (MQ=255)
 aCCGGCCCCGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGg.ggc                                                                                                      >  2:957460/1‑101 (MQ=255)
  ccGGCCCCGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGcc                                                                                                     <  1:3554226/101‑1 (MQ=255)
   cGGCCCCGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGccc                                                                                                    >  1:2281143/1‑100 (MQ=255)
    ggCCCCGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGccct                                                                                                   >  1:559109/1‑99 (MQ=255)
     gCCCCGTCTCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATc                                                                                                  <  2:4098218/101‑1 (MQ=255)
     gCCCCGTCGCCAGCCCCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATc                                                                                                  <  1:1208360/101‑1 (MQ=255)
     gCCCCGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCacc                                                                                                  >  1:337346/1‑99 (MQ=255)
     gCCCCGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGGCGAGGCGGG.CGCCATc                                                                                                  <  2:4716481/101‑1 (MQ=255)
      ccccGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.g                                                                                                <  2:3496103/101‑1 (MQ=255)
         cGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCCTC.GGCt                                                                                             >  1:4579492/1‑101 (MQ=255)
         cGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCt                                                                                             >  2:1184567/1‑101 (MQ=255)
         cGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCt                                                                                             >  1:161924/1‑101 (MQ=255)
          gTCGCCAGCCCCGGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCtt                                                                                            <  2:4522539/101‑1 (MQ=255)
          gTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCTAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCCTC.GGCtt                                                                                            >  1:4217174/1‑101 (MQ=255)
          gTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCCTC.GGCtt                                                                                            >  1:2511467/1‑101 (MQ=255)
          gTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCtt                                                                                            >  1:1118356/1‑101 (MQ=255)
             gCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCttttc                                                                                         >  1:4669253/1‑98 (MQ=255)
             gCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCttttc                                                                                         >  1:3607997/1‑98 (MQ=255)
             gCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTgcc                                                                                         <  2:4692279/101‑1 (MQ=255)
             gCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCACC.CGCttacg                                                                                         >  2:2807237/1‑98 (MQ=255)
              ccAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.GGCCAAC.GGCttttcc                                                                                        >  2:553588/1‑97 (MQ=255)
                aGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCCTC.GGCTTtccccc                                                                                      >  1:2795221/1‑101 (MQ=255)
                 gcccccGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCa                                                                                     <  1:1572609/98‑1 (MQ=255)
                  cACCGGGGTCTGAAAGCGGAACTGCGCCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCAt                                                                                    <  2:2993168/101‑1 (MQ=255)
                   cccggggTCTAAAACCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCCCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATc                                                                                   <  2:3805750/97‑1 (MQ=255)
                      cGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGACTTCGCGGTCGAGGCGGG.GGCCAACAGGCTTcccgcgcaccg                                                                                 >  2:224863/1‑95 (MQ=255)
                       gggctggAAAGCGGAACTGCGGCCCCAGCTTTTCCGGCGCATAAAGCGGGG.TGATCGCTTTCGGGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATcgcgc                                                                               <  2:1334237/95‑1 (MQ=255)
                        ggTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTttcgcccccaccgc                                                                              >  2:1422926/1‑93 (MQ=255)
                        ggTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTtccgcccccccgcc                                                                              >  2:4166931/1‑93 (MQ=255)
                          tgtGAAAGCGGAACCGCGCCCCCAGCGTTCCCAGCGAAAAAGGCGTGG.TGATCCCCTTGGGGGTCGGGGGGGG.CCCCATC.GGCTTGCCGCCATCGCGCGcc                                                                            <  1:4670893/99‑1 (MQ=255)
                            tGAAAGCGGAACGGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTc                                                                          <  1:825323/101‑1 (MQ=255)
                              aaaGGGGCACTGCGGCCCCAGCTTTCCCAGCAAAAAAAGGGTGG.GGACCGCCTTCGGGGTCGGGGGGGC.CCCCATC.GGCTTGCCGCCATCGCGCGCCTCCa                                                                        <  2:2092441/101‑1 (MQ=255)
                              aaaGCGGACCGGCGGCCCCAGGGTTTCCAGCGAATAAGGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CCCCATC.GGCTTGCCGCCATCGCGCGCCTCCa                                                                        <  2:1840330/101‑1 (MQ=255)
                                aGCGGACCTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGGGG.TGATCGCCTTCGGGGTCGGGGGGGG.CCCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGc                                                                      <  1:370477/101‑1 (MQ=255)
                                aGCGAAACCGCGTCCCCGGCGTTTCCGGCGAATAAAGCGTGG.TGATCGCCTTGGGGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGc                                                                      <  1:2865758/101‑1 (MQ=255)
                                   ggAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTTTTGCCCCCGCGCGCCTcccccccc                                                                   >  1:4165825/1‑95 (MQ=255)
                                   ggAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTTCCGCCCTCCCCCGCCCCCCCCAcc                                                                   >  2:2017134/1‑101 (MQ=255)
                                        tGCGCCCCCAGCGTTCCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CCCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGccc                                                              <  1:4252383/101‑1 (MQ=255)
                                        ggCGCCCCCAGCTTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGGGGCGGG.CCCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGccc                                                              <  1:4558590/100‑1 (MQ=255)
                                         gCGGCCCCCCCGTTCCGGGCCCATAAGCGGTGG.TGACCCCCTCCGCGGCGGAGGCGGC.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCg                                                             <  2:241060/101‑1 (MQ=255)
                                            gCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTg                                                          <  2:2024423/101‑1 (MQ=255)
                                             ccccAGCGTTTCCAGCGCATAAAGCGTGG.TGATCCCCTTCGCGGTCGGGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTgc                                                         <  2:3022786/101‑1 (MQ=255)
                                              cccGGCGTTCCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CCCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTgcg                                                        <  1:4088164/101‑1 (MQ=255)
                                              cccAGCGTTTCCAGCGCATAAAGGGTGG.TGCCCGCCTTCGCGGTCGGGGCGGC.CCCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTgcg                                                        <  1:709461/101‑1 (MQ=255)
                                                  gCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.GGCCATC.GGCTTTCCCCCCTCGCGCGGCCCCCGCACCCGCCCGCCccgcgcc                                                    >  1:4653972/1‑100 (MQ=255)
                                                    gTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATc                                                  <  2:1828797/101‑1 (MQ=255)
                                                    gTTCCCGGCGCAAAAGGCGTGG.TTACCCCCTCCGCGGTCGAGGCGGG.CCCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATc                                                  <  1:2864767/101‑1 (MQ=255)
                                                     ttcccAGCCCATAAAGGGGGG.CGACCCCCTCCTGGGTCGGGGGGGC.CCCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCg                                                 <  1:649025/98‑1 (MQ=255)
                                                     ttcccAGCCAATAAAGGGGGG.TGTGCGCCTTCGGGGTCGAGGCGGG.CCCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCg                                                 <  1:457288/98‑1 (MQ=255)
                                                        ccAGCGCATAAAGGGGGG.TGCTCCCCTCCGCGGTCGAGGCGGG.CCCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGcc                                              <  1:2687779/101‑1 (MQ=255)
                                                        ccAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTTCCGGCCTCCCGCGGCCCCCGCACCCGACCCCCGCGCGCCTcaggc                                              >  2:1853257/1‑97 (MQ=255)
                                                        ccAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGcc                                              <  1:2807402/101‑1 (MQ=255)
                                                        ccAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGcc                                              <  2:3881712/101‑1 (MQ=255)
                                                        ccAGCGCATAAAG.GTGGTTCATCGCCTTCGCGGTGGGGGCGGG.CCCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGcc                                              <  2:2652769/101‑1 (MQ=255)
                                                            cgcATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGccacca                                          <  2:161908/101‑1 (MQ=255)
                                                             gcATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTTTCGCCCTCCCCCGCCCCCCGCACCACCCCCCCGCCCGCACCCGCccccac                                         >  1:1997162/1‑101 (MQ=255)
                                                             caaaaaggggtgg.ggATCCCCTTCGCGGCGGGGGGGGG.CCCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCcaccac                                         <  1:3330229/92‑1 (MQ=255)
                                                               aTAAAGCGTGG.TGATCGNCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACAt                                       <  2:559058/101‑1 (MQ=255)
                                                                tAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTTTCGCCACCCCCCGCCCCCCCCACCCCCCCCCCGCCCGCACCCGGCCCcacaca                                      >  1:868421/1‑99 (MQ=255)
                                                                 aaggggtgt.tgCTCCCCTTCGCGGCGGGGGCGGG.CCCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAg                                     <  1:3390454/96‑1 (MQ=255)
                                                                 aaaGCGGGG.GGACCCCCTTCGCGGCCGGGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAg                                     <  1:973982/101‑1 (MQ=255)
                                                                  aaGCGTGG.TGATCCCCTTCGGGGCGGAGGCGGG.CCCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGc                                    <  2:2590014/101‑1 (MQ=255)
                                                                    gCGTGG.TTCTCGCCTTCGCGGTCGAGGCGGC.CCCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCg                                  <  2:316770/101‑1 (MQ=255)
                                                                    gCGTGG.TGCGCGCCTTCGCGTGCGCGGGGGG.CCCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCg                                  <  2:936352/101‑1 (MQ=255)
                                                                     cGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTTCCCCCCACGCGCGCCCCCCGCACCCCCCCCCCGCGCGCCTCCGCCCCcccaaaaccca                                 >  2:2701850/1‑94 (MQ=255)
                                                                     cGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGGCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGa                                 <  1:2450920/101‑1 (MQ=255)
                                                                       tgg.tgATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACa                               <  1:4229051/101‑1 (MQ=255)
                                                                       ngg.ngNNCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACa                               <  1:1700750/100‑1 (MQ=255)
                                                                       ggg.tgCTCCCCTCCGGGGCCGGGGCGGG.CCCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACa                               <  1:1235119/100‑1 (MQ=255)
                                                                        gg.tgATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTTCCGCCATCGCCCGCCTCCCGCCCCACCCCGCTCGGCGCCACGGCCACcccacaccccagct                              >  2:2163360/1‑91 (MQ=255)
                                                                         ggtgccccccTTCGGGGGGGGGGCGGG.CCCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACAt                              <  2:2386752/93‑1 (MQ=255)
                                                                         g.tgATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATc                             <  2:2511322/101‑1 (MQ=255)
                                                                         g.tgATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.AGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATc                             <  1:4104748/101‑1 (MQ=255)
                                                                           tgATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTTCCGCCCCCCCGCGCCCCCCCCCCCCACCCCCCGCCCGCCTCGGCCCCCCCCAAGCCCACaacc                            >  1:1318249/1‑98 (MQ=255)
                                                                           tgATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATcg                            <  1:3659847/101‑1 (MQ=255)
                                                                           tgATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATcg                            <  2:1507274/101‑1 (MQ=255)
                                                                              tCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATcgccg                         <  2:2650978/101‑1 (MQ=255)
                                                                               cGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATcgccgc                        <  1:553639/101‑1 (MQ=255)
                                                                               cGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCCCCCACCGGCGCCCCCAACCCCCACCCCCTACGCGCACCGGCCCCCCCACAGCCCACACcgcccc                        >  2:310245/1‑99 (MQ=255)
                                                                                gCCTTCCCGG.CGGGGCGGGCCCCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCgccgcc                       <  2:379452/101‑1 (MQ=255)
                                                                                  cTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCa                     <  1:1423020/101‑1 (MQ=255)
                                                                                  cTTCGCGGTCGAGGCGGG.CGCCATC.GCCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCa                     <  1:2854527/101‑1 (MQ=255)
                                                                                   ttCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCCCCACATAGCCGACATCGCCGCCCAg                    <  1:1382872/101‑1 (MQ=255)
                                                                                   ttCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCAg                    <  2:4216927/101‑1 (MQ=255)
                                                                                   ttCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCAg                    <  2:3477178/101‑1 (MQ=255)
                                                                                    tCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCGGCACCAGCCCGCTGCGCGCATCGGCCACCAGATAGCCGACATCGCCGCCCAgc                   <  2:2692600/101‑1 (MQ=255)
                                                                                       cgGTCGAGGCGGG.CGCCATC.GGCTTTCCCCCCCCGCGCGCCCCCCCCACCACCCCCCCGCGCGCCTCGGGCCCCCCCTAGCCGACACCCCCCccccccccc                >  1:3582203/1‑95 (MQ=255)
                                                                                           cGAGGCGGG.CGCCATC.GGCTTTCCGCCATCGCGCGCCCCCCGCACCCGCCCCCCGCGCGCATCGGCCCCCACCTAGCCGACATCCCCCCCCCgccgccccg            >  2:2807670/1‑101 (MQ=255)
                                                                                            gAGGCGGG.CGCCATC.GGCTTTCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCCgccgcgccgc           >  1:608419/1‑101 (MQ=255)
                                                                                            gAGGCGGG.CGCCATC.GGCTTTCCGCCATCGCGCGCCCCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCCgccgcgccgc           >  2:1048704/1‑101 (MQ=255)
                                                                                             aGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGGACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCCCCCACGCGcggcgcc          >  2:2199804/1‑101 (MQ=255)
                                                                                             aGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCAGCCGcgccgcc          <  1:375495/101‑1 (MQ=255)
                                                                                               gcggg.cgCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCAGCCGcgccgccgc        <  2:3607778/101‑1 (MQ=255)
                                                                                               gcggg.cgCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCAGCCGcgccgccgc        <  1:957537/101‑1 (MQ=255)
                                                                                                 ggg.cgCCATC.GGCTTGCCGCCATCCCGGGCCTCCACCACCATCCCGCTTCGCGCCTCCCCCACCACATAACCGACATCGCCGCCCGGGCTCCCCGCcgcgt      >  2:1127596/1‑100 (MQ=255)
                                                                                                   g.cgcCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCAGCCGCGCCGCCGCGATc    <  2:4376686/101‑1 (MQ=255)
                                                                                                     cgcCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCAGCCGCGCCGCCGCGATCa   <  2:1985175/101‑1 (MQ=255)
                                                                                                      gcCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCCGCCGCGCCGCCGCGACCAg  >  1:4503455/1‑101 (MQ=255)
                                                                                                      gcCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCAGCCGCGCCGCCGCGATCAg  >  1:2665779/1‑101 (MQ=255)
                                                                                                      |                                                                                                     
GACCGGCCCCGTCGCCAGCACCCGGGTCTGAAAGCGGAACTGCGGCCCCAGCGTTTCCAGCGCATAAAGCGTGG.TGATCGCCTTCGCGGTCGAGGCGGG.CGCCATC.GGCTTGCCGCCATCGCGCGCCTCCAGCACCAGCCCGCTGCGCGCATCGGCCACCACATAGCCGACATCGCCGCCCAGCCGCGCCGCCGCGATCAG  >  NC_014034/2819568‑2819768

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG