Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,891,981 T→G 100% S460A (TCG→GCG)  thiP → thiamine ABC transporter permease

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,891,9810T→G100.0% 821.9 221S460A (TCG→GCG) thiPthiamine ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (111/110):  ref base (0/0):  total (111/110)

Only 100 of 231 total aligned reads displayed.

CCCGGCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCTCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGCCT  >  NC_014034/2891883‑2892078
                                                                                                  |                                                                                                 
cccGGCTGCGGTTCCTCCCCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCg                                                                                                 <  1:2216267/101‑1 (MQ=255)
 ccGGCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGc                                                                                                >  2:598262/1‑101 (MQ=255)
 ccGGCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGc                                                                                                <  1:162921/101‑1 (MQ=255)
    gCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGt                                                                                             >  2:626955/1‑101 (MQ=255)
      tGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGttt                                                                                           >  2:498324/1‑101 (MQ=255)
        cGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTc                                                                                         >  1:4597690/1‑101 (MQ=255)
         ggTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTcc                                                                                        >  2:1057562/1‑101 (MQ=255)
         ggTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTcc                                                                                        <  2:747579/101‑1 (MQ=255)
          gTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCg                                                                                       <  1:3997298/101‑1 (MQ=255)
          gTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCg                                                                                       <  2:2773342/101‑1 (MQ=255)
           ttCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGa                                                                                      >  2:1695103/1‑101 (MQ=255)
           ttCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTGCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGa                                                                                      >  1:4009817/1‑101 (MQ=255)
            tCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGAt                                                                                     >  2:3946026/1‑101 (MQ=255)
              cTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGTTCTGGACGCCATCGCGCTGTTTTCCGAtcg                                                                                   >  2:2816734/1‑99 (MQ=255)
                 ccccTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCg                                                                                <  1:3214991/100‑1 (MQ=255)
                  cccTTCCCCGGCTGGCCCGGCCGACGATCTTTGCCGCAGTAATTGCCTCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGc                                                                               <  2:3064991/101‑1 (MQ=255)
                   ccTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCa                                                                              <  2:4695228/101‑1 (MQ=255)
                     tGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGc                                                                            >  1:4692041/1‑101 (MQ=255)
                     tGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGc                                                                            >  2:1839004/1‑101 (MQ=255)
                     ggCCCCGGCTGGCGCTGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGc                                                                            <  1:4498642/100‑1 (MQ=255)
                      gCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGcc                                                                           >  1:2280316/1‑101 (MQ=255)
                       ccccGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCa                                                                          >  1:2939615/1‑101 (MQ=255)
                         ccGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGa                                                                        <  1:1158229/101‑1 (MQ=255)
                         ccGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGa                                                                        <  1:598326/101‑1 (MQ=255)
                         ccGGCNNGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGa                                                                        <  1:2012713/101‑1 (MQ=255)
                           ggCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGAcc                                                                      >  2:4386913/1‑101 (MQ=255)
                            gCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGGCATCGCGCTGTTTTCCGATGGCCGCAGCCAGAccc                                                                     >  1:2551980/1‑101 (MQ=255)
                              tGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTg                                                                   >  2:131976/1‑101 (MQ=255)
                                 cccGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCg                                                                <  1:3616336/101‑1 (MQ=255)
                                  ccGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCTCTGTTTTCAAATGAGCGCAGCCAGACCATGACGc                                                               <  2:143558/101‑1 (MQ=255)
                                   cGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCt                                                              <  1:3857462/101‑1 (MQ=255)
                                    ggCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCtt                                                             <  2:115064/101‑1 (MQ=255)
                                    ggCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCtt                                                             <  1:2873/101‑1 (MQ=255)
                                       cGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGcc                                                          >  1:4280529/1‑101 (MQ=255)
                                         aCGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGt                                                        >  1:1307429/1‑101 (MQ=255)
                                         aCGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGt                                                        >  1:948058/1‑101 (MQ=255)
                                          cGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTc                                                       >  1:2222949/1‑101 (MQ=255)
                                           gggCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCt                                                      <  1:498375/101‑1 (MQ=255)
                                            ggCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTa                                                     <  1:3452659/101‑1 (MQ=255)
                                            ggCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTa                                                     <  1:4370088/101‑1 (MQ=255)
                                            ggCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTa                                                     <  2:240108/101‑1 (MQ=255)
                                             gCTTTGCCGGGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGTCCGCAGCCAGACCCTGCCGCTTGCCGTCTAt                                                    <  2:2359064/101‑1 (MQ=255)
                                             gCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTAt                                                    >  2:3704190/1‑101 (MQ=255)
                                             gCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTAt                                                    <  2:2143100/101‑1 (MQ=255)
                                             gCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGGTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTAt                                                    <  1:3535973/101‑1 (MQ=255)
                                              cTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATc                                                   <  1:491795/101‑1 (MQ=255)
                                               tttGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCa                                                  >  1:650011/1‑101 (MQ=255)
                                               tttGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCa                                                  <  2:2162107/101‑1 (MQ=255)
                                               tttGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCa                                                  <  2:4597437/101‑1 (MQ=255)
                                                 tGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGc                                                >  2:1403908/1‑101 (MQ=255)
                                                 tGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGc                                                <  1:2776715/101‑1 (MQ=255)
                                                 tGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGc                                                >  1:3265701/1‑101 (MQ=255)
                                                  gCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCt                                               >  1:341081/1‑101 (MQ=255)
                                                  gCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCt                                               >  2:3540304/1‑101 (MQ=255)
                                                  gCCGCGGGGATTGCCGCGGCCGTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCt                                               >  1:2697668/1‑101 (MQ=255)
                                                   ccGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTg                                              >  1:4167186/1‑101 (MQ=255)
                                                     gcgGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGAt                                            >  1:2141693/1‑101 (MQ=255)
                                                      cgGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATg                                           >  2:4072469/1‑101 (MQ=255)
                                                      cgGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATg                                           <  2:649947/101‑1 (MQ=255)
                                                       ggggATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATgg                                          <  1:627019/101‑1 (MQ=255)
                                                         ggATGGCCGCGGCCTTTTCGATGGGCAATCTGGCCGCCATCGCCCTGTTTTCCGATGGCCGCAGCCAAACCCTGCCGCTTGCCGTCTATCAGCTGATGGGc                                        <  2:1195742/101‑1 (MQ=255)
                                                           agtGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAg                                      <  1:3133370/99‑1 (MQ=255)
                                                             tGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGCCCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCt                                    <  2:3135089/101‑1 (MQ=255)
                                                             tGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCt                                    >  1:2661407/1‑101 (MQ=255)
                                                              gCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTa                                   <  2:1987537/101‑1 (MQ=255)
                                                               ccGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTAt                                  <  2:2792270/101‑1 (MQ=255)
                                                               ccGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTAt                                  <  1:3061691/101‑1 (MQ=255)
                                                               ccGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTAt                                  <  2:772263/101‑1 (MQ=255)
                                                                cgcgGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATc                                 <  2:341047/101‑1 (MQ=255)
                                                                  cgGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGc                               <  2:2218409/101‑1 (MQ=255)
                                                                   ggCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCa                              <  2:1803081/101‑1 (MQ=255)
                                                                     ccTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATg                            <  2:4211547/101‑1 (MQ=255)
                                                                     ccTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATg                            <  2:4578841/101‑1 (MQ=255)
                                                                       ttttCGATGGGCGATCTGGGCGCCATCGCGTTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGa                          <  2:1271824/101‑1 (MQ=255)
                                                                       ttttCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGa                          >  1:1080775/1‑101 (MQ=255)
                                                                        tttCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGAt                         <  1:76526/101‑1 (MQ=255)
                                                                        tttCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGAt                         >  1:4134550/1‑101 (MQ=255)
                                                                          tCGCTGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCa                       >  2:453581/1‑101 (MQ=255)
                                                                          tCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCa                       <  1:4422916/101‑1 (MQ=255)
                                                                           cGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAg                      <  1:1017948/101‑1 (MQ=255)
                                                                           cGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAg                      <  1:4711888/101‑1 (MQ=255)
                                                                            gATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAgg                     >  1:1195425/1‑101 (MQ=255)
                                                                             aTGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGc                    >  1:978922/1‑101 (MQ=255)
                                                                             aTGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGc                    <  1:389574/101‑1 (MQ=255)
                                                                              tGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGcc                   >  2:376017/1‑101 (MQ=255)
                                                                              tGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGcc                   >  1:3788198/1‑101 (MQ=255)
                                                                               gggCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCcg                  >  1:3005364/1‑101 (MQ=255)
                                                                               gggCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAACTGATGGGCAGCTATCGCATGGATCAGGCcg                  >  1:3935006/1‑101 (MQ=255)
                                                                                   gATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCggg              <  1:2805017/101‑1 (MQ=255)
                                                                                    aTCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGgc             >  2:4184429/1‑101 (MQ=255)
                                                                                     tCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGgcg            <  1:1670497/101‑1 (MQ=255)
                                                                                      cTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGgcgc           <  2:3265500/101‑1 (MQ=255)
                                                                                       tGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCGGCTATCGCATGGATCAGGCCGCGGGcgcg          >  1:4572721/1‑101 (MQ=255)
                                                                                       tGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGcgcg          >  2:4691343/1‑101 (MQ=255)
                                                                                       tGGGCGCCATCGCGCTGTTTTCCGATGGCCGAAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGcgcg          >  1:4210822/1‑101 (MQ=255)
                                                                                          gcgcCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCt       >  1:1894677/1‑101 (MQ=255)
                                                                                           cgcCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCtt      <  2:3904193/101‑1 (MQ=255)
                                                                                           cgcCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCtt      <  2:2852820/101‑1 (MQ=255)
                                                                                           cgcCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCtt      >  2:936310/1‑101 (MQ=255)
                                                                                              cATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGcc   >  1:2327734/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CCCGGCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCTCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGCCT  >  NC_014034/2891883‑2892078

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG