Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,714 C→G 1.9% intergenic (+57/‑103) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7140C→G 1.9% 3.5 ‑1.7 ‑0.4267intergenic (+57/‑103)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (144/117):  total (146/121)
Fisher's exact test strand distribution p-value = 1.81e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.26e-01

Only 100 of 288 total aligned reads displayed.

CCGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAA  >  NC_014034/2950614‑2950814
                                                                                                     |                                                                                                     
ccGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAacac                                                                                                       >  1:814130/1‑101 (MQ=255)
 cGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACg                                                                                                      <  1:4394794/101‑1 (MQ=255)
  ggggTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGc                                                                                                     >  1:3667563/1‑101 (MQ=255)
   gggTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGcc                                                                                                    <  2:3740638/101‑1 (MQ=255)
    ggTGCCGCCCACGCCGCAATATGCGGACCTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGccc                                                                                                   <  1:1798686/101‑1 (MQ=255)
     gTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGcccc                                                                                                  <  2:3070234/101‑1 (MQ=255)
     gTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGcccc                                                                                                  <  2:3809916/101‑1 (MQ=255)
     gTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGcccc                                                                                                  <  1:1728583/101‑1 (MQ=255)
      tGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCt                                                                                                 >  2:1672076/1‑101 (MQ=255)
      ggccgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCt                                                                                                 <  2:2417159/100‑1 (MQ=255)
        ccgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCttt                                                                                               <  2:2978708/101‑1 (MQ=255)
          gccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCa                                                                                             <  1:3358880/101‑1 (MQ=255)
          gccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCa                                                                                             >  2:3395162/1‑101 (MQ=255)
            ccTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATc                                                                                           <  2:4512079/101‑1 (MQ=255)
             cTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCt                                                                                          <  2:2783058/101‑1 (MQ=255)
                  cgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGccc                                                                                     <  2:1277338/101‑1 (MQ=255)
                   gcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGcccc                                                                                    <  2:5805/101‑1 (MQ=255)
                   gcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGcccc                                                                                    >  1:637741/1‑101 (MQ=255)
                    cAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCTGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCa                                                                                   <  2:4624392/101‑1 (MQ=255)
                    cAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATTTTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCa                                                                                   >  2:459552/1‑101 (MQ=255)
                    cAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCa                                                                                   <  1:2777654/101‑1 (MQ=255)
                    cAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCa                                                                                   >  2:1013549/1‑101 (MQ=255)
                    cAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCa                                                                                   <  1:3194147/101‑1 (MQ=255)
                     aaTATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaa                                                                                  >  2:3244831/1‑101 (MQ=255)
                      atatGCGGAACTTGATTTCTGAATCAAGCCGGGGGCTCTGCCCCCCGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaaa                                                                                 <  1:3142887/101‑1 (MQ=255)
                         tGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAatat                                                                              <  2:3304193/101‑1 (MQ=255)
                          gCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATc                                                                             >  2:2719768/1‑101 (MQ=255)
                            ggAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATccc                                                                           <  1:1017163/101‑1 (MQ=255)
                               aCTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.ggg                                                                       <  2:301871/101‑1 (MQ=255)
                                 ttGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.ggggg                                                                     <  2:1443500/101‑1 (MQ=255)
                                 ttGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.ggggg                                                                     <  1:4506723/101‑1 (MQ=255)
                                   gATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTg                                                                   >  1:2185106/1‑101 (MQ=255)
                                     tttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.TAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGaa                                                                 >  1:161227/1‑101 (MQ=255)
                                     tttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGaa                                                                 <  1:1664449/101‑1 (MQ=255)
                                     tttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGaa                                                                 >  1:1595981/1‑101 (MQ=255)
                                       tCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAtt                                                               <  1:2950286/101‑1 (MQ=255)
                                       tCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAtt                                                               <  2:464121/101‑1 (MQ=255)
                                        cTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAttt                                                              >  2:4573452/1‑101 (MQ=255)
                                         tGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTg                                                             >  1:4666342/1‑101 (MQ=255)
                                            agcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCg                                                          >  1:1199970/1‑101 (MQ=255)
                                            agcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCg                                                          <  2:2557145/101‑1 (MQ=255)
                                             gcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGt                                                         >  1:4527545/1‑101 (MQ=255)
                                              caagcCGGGGGCTCTGCCCCCGGACCCCCGGGACATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGGCCCAAATATCCC.GGGGGTGAATTTGCCGTc                                                        >  1:1889350/1‑101 (MQ=255)
                                               aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                       <  2:2469831/101‑1 (MQ=255)
                                               aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                       <  2:4310164/101‑1 (MQ=255)
                                                  ccGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGc                                                    <  2:3179684/101‑1 (MQ=255)
                                                    gggggCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaa                                                  >  2:1194439/1‑101 (MQ=255)
                                                    gggggCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaa                                                  <  2:814057/101‑1 (MQ=255)
                                                    gggggCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaa                                                  >  1:4186376/1‑101 (MQ=255)
                                                      gggCTCTGCCCCCGGTCCCCCCGGATTTTTGGGGGCAAGATGAAACCCGCCCCTTTCATCTTG.CCCAAATTTTCC.GGGGGTGAATTTGCCGTCAGGCAAag                                                <  2:1106538/101‑1 (MQ=21)
                                                      gggCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAag                                                >  2:2896414/1‑101 (MQ=255)
                                                       ggCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAaga                                               <  2:2861373/101‑1 (MQ=255)
                                                       ggCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAaga                                               <  2:1620731/101‑1 (MQ=255)
                                                        gCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAagag                                              <  1:1992121/101‑1 (MQ=255)
                                                            tGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAggggg                                          >  2:1804674/1‑101 (MQ=255)
                                                             ttcgcctcaCCCCCGGGATATTT.GGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAggggggc                                        >  1:3681076/9‑99 (MQ=25)
                                                             ttcgcctcaCCCCCGGGATATTT.GGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAggggggc                                        >  1:4116521/9‑99 (MQ=25)
                                                             gCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAAAgggggg                                         >  1:155802/1‑100 (MQ=255)
                                                             gCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCAAAGAgggggg                                         >  2:4065671/1‑100 (MQ=255)
                                                              tcgcctcaCCCCCGGGATATTT.GGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAggggggca                                       <  2:3665917/94‑4 (MQ=25)
                                                              cccccGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCa                                        <  2:2111587/101‑1 (MQ=255)
                                                               cgcctcaCCCCCGGGATATTT.GGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAggggggcag                                      >  2:2347200/7‑97 (MQ=25)
                                                                 cgctccccccGGGATATTT.GCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAGcgc                                    <  2:3215774/96‑3 (MQ=2)
                                                                  cGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTc                                    >  2:439562/1‑101 (MQ=255)
                                                                   ggACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTcc                                   >  2:1540921/1‑101 (MQ=255)
                                                                   ctccccccGGGATATTT.GCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAGCGccc                                  >  2:2696761/4‑101 (MQ=2)
                                                                    tccccccGGGATATTT.GCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAGCGcccc                                 <  1:3740144/99‑1 (MQ=2)
                                                                    gACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTccc                                  >  1:2382176/1‑101 (MQ=255)
                                                                    gACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTccc                                  >  1:3589380/1‑101 (MQ=255)
                                                                     aCCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTcccc                                 >  1:2688823/1‑101 (MQ=255)
                                                                     aCCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTcccc                                 >  1:4271625/1‑101 (MQ=255)
                                                                        cccGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTg                              <  1:3395368/101‑1 (MQ=255)
                                                                         ccGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGc                             <  1:3066300/101‑1 (MQ=255)
                                                                           gggATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGccc                           >  2:2756046/1‑101 (MQ=255)
                                                                             gATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCg                         <  1:1430137/101‑1 (MQ=255)
                                                                             gATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCg                         >  1:491/1‑101 (MQ=255)
                                                                               tatTT.GAGGCAAGATCAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGc                       <  2:1877120/101‑1 (MQ=255)
                                                                                 ttt.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTg                     <  2:4186130/101‑1 (MQ=255)
                                                                                 ttt.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTg                     >  2:13493/1‑101 (MQ=255)
                                                                                 ttt.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCAC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTg                     >  2:4103708/1‑101 (MQ=255)
                                                                                  tt.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTga                    <  2:2768714/101‑1 (MQ=255)
                                                                                  tt.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTga                    <  2:2188631/101‑1 (MQ=255)
                                                                                  tt.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTga                    <  2:1595881/101‑1 (MQ=255)
                                                                                   t.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgag                   >  1:3422593/1‑101 (MQ=255)
                                                                                     gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagg                  >  2:928183/1‑101 (MQ=255)
                                                                                     gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTACCCCTGCCCCGGCTgagg                  >  1:52034/1‑101 (MQ=255)
                                                                                      aGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggg                 >  2:3528080/1‑101 (MQ=255)
                                                                                       ggCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga                <  2:2363570/101‑1 (MQ=255)
                                                                                        gCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagggag               <  1:2727203/101‑1 (MQ=255)
                                                                                            gATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCgg           <  1:459596/101‑1 (MQ=255)
                                                                                             aTGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGa          >  1:1120022/1‑101 (MQ=255)
                                                                                             aTGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGa          <  2:477028/101‑1 (MQ=255)
                                                                                              tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa         >  1:2305734/1‑101 (MQ=255)
                                                                                                aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAcg       >  1:2767068/1‑100 (MQ=255)
                                                                                                aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt       >  2:3118396/1‑101 (MQ=255)
                                                                                                 aaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTg      >  1:3973281/1‑101 (MQ=255)
                                                                                                    ccGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaa   <  1:897949/100‑1 (MQ=255)
                                                                                                    acGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaa   <  1:3814400/101‑1 (MQ=255)
                                                                                                     cGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaaa  >  1:2550916/1‑101 (MQ=255)
                                                                                                     cGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaaa  >  2:692608/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
CCGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAA  >  NC_014034/2950614‑2950814

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG