Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,743 G→A 1.7% intergenic (+86/‑74) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7430G→A 1.7% 4.6 ‑1.8 ‑0.5286intergenic (+86/‑74)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (165/116):  total (166/120)
Fisher's exact test strand distribution p-value = 1.65e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.27e-01

Only 100 of 321 total aligned reads displayed.

GAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCC  >  NC_014034/2950643‑2950843
                                                                                                      |                                                                                                       
gAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.g                                                                                                         <  2:2870053/101‑1 (MQ=255)
 aaCTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.gg                                                                                                        >  1:3218478/1‑101 (MQ=255)
 aaCTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.gg                                                                                                        >  1:2038822/1‑101 (MQ=255)
  aCTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGTTATTT.GAGGCAAGATGAAACACGCCCATTTCATCTTGCCCCAAATATCCC.ggg                                                                                                       >  2:3048645/1‑101 (MQ=255)
    ttGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.gggg..g                                                                                                   <  1:4506723/101‑1 (MQ=255)
     tGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..Gt                                                                                                  >  1:2747834/1‑101 (MQ=255)
      gATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTg                                                                                                 >  1:2185106/1‑101 (MQ=255)
       aTTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGa                                                                                                <  2:2466163/101‑1 (MQ=255)
        tttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGaa                                                                                               <  1:1664449/101‑1 (MQ=255)
        tttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGaa                                                                                               >  2:1342973/1‑101 (MQ=255)
        tttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GGGaa                                                                                               >  1:1595981/1‑101 (MQ=255)
         ttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAAt                                                                                              >  1:1913584/1‑101 (MQ=255)
         ttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GGGAAt                                                                                              >  2:4198827/1‑101 (MQ=255)
         ttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCA.GGGG..GCGAAt                                                                                              >  2:1157164/1‑101 (MQ=255)
          tCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAAtt                                                                                             <  1:2950286/101‑1 (MQ=255)
          tCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAAtt                                                                                             <  2:464121/101‑1 (MQ=255)
              aagcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.Gcc                                                                                        >  2:3929580/1‑101 (MQ=255)
                gcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGt                                                                                      >  1:417555/1‑101 (MQ=255)
                gcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGt                                                                                      >  2:3011852/1‑101 (MQ=255)
                 caagcCGGGGGCTCTGCCCCCGGACCCCCGGGGTATTT.TGGGCAAAAAGAAAACCCCCCCCTTTATCTTCCCCCCAAAATCCC.GGGG..GGGGATTT.GCCGTc                                                                                     >  1:3383289/1‑101 (MQ=9)
                  gagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCa                                                                                    >  2:2899636/2‑101 (MQ=255)
                  aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCa                                                                                    >  2:276749/1‑101 (MQ=255)
                  aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCa                                                                                    <  2:3580597/101‑1 (MQ=255)
                    gcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAgg                                                                                  <  2:3218277/101‑1 (MQ=255)
                      cGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCa                                                                                <  2:2709753/101‑1 (MQ=255)
                      cGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GCGG..GTGAATTT.GCCGTCAGGCa                                                                                <  1:2700150/101‑1 (MQ=255)
                       gggggCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCaa                                                                               <  2:814057/101‑1 (MQ=255)
                         gggCTCTGCCCCCGGTCCCCCCGGATTTTTGGGGGCAAGATGAAACCCGCCCCTTTCATCTTG.CCCAAATTTTCC.GGGG..GTGAATTT.GCCGTCAGGCAAag                                                                             <  2:1106538/101‑1 (MQ=21)
                          ggCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAaga                                                                            <  2:1619554/101‑1 (MQ=255)
                           gCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAagag                                                                           <  1:3051749/101‑1 (MQ=255)
                              ctGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GGGAATTT.GCCGTCAGGCAAAGAgggg                                                                        >  1:810906/1‑101 (MQ=255)
                               cttcgcctcaCCCCCGGGATATTT.GGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGG..GTGAATTG.GCCG.AAGGCCAAGAgggggg                                                                      >  2:2067505/10‑100 (MQ=25)
                                ttcgcctcaCCCCCGGGATATTT.GGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGG..GTGAATTG.GCCG.AAGGCCAAGAggggggc                                                                     >  1:3681076/9‑99 (MQ=25)
                                 cccccGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCa                                                                     <  2:1803456/101‑1 (MQ=255)
                                     cGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTc                                                                 >  2:439562/1‑101 (MQ=255)
                                      ggACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTcc                                                                >  2:1540921/1‑101 (MQ=255)
                                       tccccccGGGATATTT.GCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGG..GTGAATTG.GCC.AAAGGCCAAGAGGGGGCAGCGcccc                                                              <  1:3740144/99‑1 (MQ=2)
                                       gACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTccc                                                               >  1:3589380/1‑101 (MQ=255)
                                       gACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTccc                                                               >  1:2382176/1‑101 (MQ=255)
                                        aCCCCCGGTATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTcccc                                                              <  1:263052/101‑1 (MQ=255)
                                          ccccGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTccccca                                                            >  1:2491509/1‑100 (MQ=255)
                                          ccccGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCt                                                            <  2:3211393/101‑1 (MQ=255)
                                                gATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCg                                                      >  1:4239608/1‑101 (MQ=255)
                                                 atatTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCgg                                                     >  2:4378238/1‑101 (MQ=255)
                                                 atatTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCgg                                                     >  2:2635041/1‑101 (MQ=255)
                                                 atatTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGCCGGGCAAAGAGGGGGCAGCTTCCCCGGCCCCgg                                                     >  1:1140624/1‑101 (MQ=25)
                                                  tatTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGc                                                    <  2:3417831/101‑1 (MQ=255)
                                                  tatTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGc                                                    <  2:2771842/101‑1 (MQ=255)
                                                    ttt.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCAC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTg                                                  >  2:4103708/1‑101 (MQ=255)
                                                     tt.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTga                                                 >  1:4616179/1‑101 (MQ=255)
                                                     tt.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTga                                                 <  2:1595881/101‑1 (MQ=255)
                                                      t.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgag                                                >  2:3299602/1‑101 (MQ=255)
                                                      t.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgag                                                >  1:3422593/1‑101 (MQ=255)
                                                        gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagg                                               >  2:928183/1‑101 (MQ=255)
                                                          ggCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga                                             >  1:3140171/1‑101 (MQ=255)
                                                          ggCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga                                             <  1:4524893/101‑1 (MQ=255)
                                                               gATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCgg                                        <  1:459596/101‑1 (MQ=255)
                                                               gATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCgg                                        >  2:2195624/1‑101 (MQ=255)
                                                                aTGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGa                                       >  1:4748811/1‑101 (MQ=255)
                                                                aTGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGa                                       <  1:142613/101‑1 (MQ=255)
                                                                 tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa                                      <  1:1808975/101‑1 (MQ=255)
                                                                 tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa                                      >  1:2305734/1‑101 (MQ=255)
                                                                  gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc                                     >  2:238896/1‑101 (MQ=255)
                                                                   aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt                                    >  2:3118396/1‑101 (MQ=255)
                                                                    aaccccccccTTTCCACTTTCCCCAAATTTCCCGGGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt                                    <  1:3288587/95‑1 (MQ=25)
                                                                       ccGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaa                                <  1:897949/100‑1 (MQ=255)
                                                                       acGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaa                                <  1:3814400/101‑1 (MQ=255)
                                                                        cGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaaa                               >  2:692608/1‑101 (MQ=255)
                                                                         gCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAGGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAc                              >  1:3889082/1‑101 (MQ=255)
                                                                          ccccTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAcc                             <  1:525525/101‑1 (MQ=255)
                                                                             cTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg                          <  2:4584702/101‑1 (MQ=255)
                                                                              tttCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGa                         >  2:555321/1‑101 (MQ=255)
                                                                              tttCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGa                         >  2:4511562/1‑101 (MQ=255)
                                                                               ttcccccccccccAAAATGCCC.GGGG..ATGTATTTCGCCGGCAGAAAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGa                         <  1:2768811/92‑1 (MQ=255)
                                                                               ttCATCTCCCCCCAATGAGCGG.GGGG..GTGAATTC.CCGGACGGGAAAGGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGaa                        <  2:4615926/101‑1 (MQ=255)
                                                                                tCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg                       <  1:1541022/101‑1 (MQ=255)
                                                                                tCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg                       <  2:1082087/101‑1 (MQ=255)
                                                                                tCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg                       >  2:4546909/1‑101 (MQ=255)
                                                                                 cATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGa                      >  1:413088/1‑101 (MQ=255)
                                                                                 cATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGa                      >  2:1850804/1‑101 (MQ=255)
                                                                                 cATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGa                      <  2:1913472/101‑1 (MQ=255)
                                                                                 cATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGa                      >  2:2319725/1‑101 (MQ=255)
                                                                                    cTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc                   >  2:1679040/1‑101 (MQ=255)
                                                                                      ctccccAAAAATCCC.GGGGGTGTGAATTC.GCCGAGAGAAAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc                   <  2:3655313/99‑1 (MQ=255)
                                                                                        ccccAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCt               <  1:1001811/101‑1 (MQ=255)
                                                                                         cccAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTg              <  2:4036686/101‑1 (MQ=255)
                                                                                            aaacagccc.GGGG..ATCAATTT.GCCGCCAGGCAAATAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc           <  2:2362596/95‑1 (MQ=255)
                                                                                            aaaTATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc           >  2:4249134/1‑101 (MQ=255)
                                                                                            aaaTATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc           >  1:1502308/1‑101 (MQ=255)
                                                                                             aaTATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccagatcggaag          >  1:1612850/1‑91 (MQ=255)
                                                                                             aaTATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg          >  2:1349904/1‑101 (MQ=255)
                                                                                              atatCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGa         <  1:2896590/101‑1 (MQ=255)
                                                                                              atatCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGa         >  1:4311732/1‑101 (MQ=255)
                                                                                               tgtCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa        <  1:1703800/99‑1 (MQ=255)
                                                                                               tatCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGATGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa        <  1:4065913/101‑1 (MQ=255)
                                                                                               tatCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa        >  2:3744686/1‑101 (MQ=255)
                                                                                                 tCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAaga      <  1:664633/101‑1 (MQ=255)
                                                                                                  gggggggg..gTGATTTG.GCCGGGAGGAAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAaga      <  2:2550764/97‑1 (MQ=255)
                                                                                                    c.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGc   >  2:1456889/1‑101 (MQ=255)
                                                                                                      gggg..gTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc  >  1:1227201/1‑101 (MQ=255)
                                                                                                      |                                                                                                       
GAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCC  >  NC_014034/2950643‑2950843

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG