Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,754 T→G 4.2% intergenic (+97/‑63) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7540T→G 4.2% 24.5 ‑0.6 ‑1.3284intergenic (+97/‑63)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (6/6):  ref base (163/109):  total (169/115)
Fisher's exact test strand distribution p-value = 5.55e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.38e-02

Only 100 of 312 total aligned reads displayed.

CTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGG  >  NC_014034/2950654‑2950854
                                                                                                      |                                                                                                      
      caagcCGGGGGCTCTGCCCCCGGACCCCCGGGGTATTT.TGGGCAAAAAGAAAACCCCCCCCTTTATCTTCCCCCCAAAATCCC.GGGGGGGGATTT.GCCGTc                                                                                                 >  1:3383289/1‑101 (MQ=9)
       aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGACACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCa                                                                                                >  2:2808219/1‑101 (MQ=255)
       aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCa                                                                                                <  2:3580597/101‑1 (MQ=255)
         gcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAgg                                                                                              <  2:909018/101‑1 (MQ=255)
         gcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAgg                                                                                              <  2:3218277/101‑1 (MQ=255)
         gcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAgg                                                                                              <  1:1517517/101‑1 (MQ=255)
           cGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCa                                                                                            <  2:2709753/101‑1 (MQ=255)
           cGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTT.GCCGTCAGGCa                                                                                            >  1:1745077/1‑101 (MQ=255)
            gggggCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCaa                                                                                           >  2:4607829/1‑101 (MQ=255)
            gggggCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCaa                                                                                           >  2:1194439/1‑101 (MQ=255)
            gggggCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCaa                                                                                           >  1:4186376/1‑101 (MQ=255)
            gggggCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTT.GCCGTCAGGcca                                                                                           >  1:943798/1‑99 (MQ=255)
              gggCTCTGCCCCCGGTCCCCCCGGATTTTTGGGGGCAAGATGAAACCCGCCCCTTTCATCTTG.CCCAAATTTTCC.GGGGGTGAATTT.GCCGTCAGGCAAag                                                                                         <  2:1106538/101‑1 (MQ=21)
               ggCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAaga                                                                                        <  2:1620731/101‑1 (MQ=255)
                gCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAagag                                                                                       <  1:1992121/101‑1 (MQ=255)
                gCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAagag                                                                                       <  1:3051749/101‑1 (MQ=255)
                 ctctGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAgg                                                                                      <  2:1607431/101‑1 (MQ=255)
                   ctGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCGTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAgggg                                                                                    >  2:4102474/1‑101 (MQ=255)
                   ctGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAgggg                                                                                    >  2:1767295/1‑101 (MQ=255)
                      cccccGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCa                                                                                <  2:1381820/101‑1 (MQ=255)
                       cgcctcaCCCCCGGGATATTT.GGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTG.GCCG.AAGGCCAAGAgggg.ggcag                                                                              >  2:2347200/7‑97 (MQ=25)
                       ccccGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAg                                                                               <  1:2420912/101‑1 (MQ=255)
                        cccGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGc                                                                              >  2:509551/1‑101 (MQ=255)
                          cGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTc                                                                            >  1:4526217/1‑101 (MQ=255)
                          cGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTc                                                                            >  1:1864579/1‑101 (MQ=255)
                          cGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTc                                                                            >  2:439562/1‑101 (MQ=255)
                           ggACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTcc                                                                           <  2:1344659/101‑1 (MQ=255)
                           ggACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTcc                                                                           <  2:1546001/101‑1 (MQ=255)
                           ggACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTcc                                                                           >  1:2363703/1‑101 (MQ=255)
                           ggACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTcc                                                                           <  2:2891914/101‑1 (MQ=255)
                            tccccccGGGATATTT.GCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTG.GCC.AAAGGCCAAGAGGGG.GCAGCGcccc                                                                         <  1:1608539/99‑1 (MQ=2)
                             aCCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTcccc                                                                         >  2:2131114/1‑101 (MQ=255)
                             aCCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTcccc                                                                         >  1:2688823/1‑101 (MQ=255)
                             aCCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTcccc                                                                         >  1:4271625/1‑101 (MQ=255)
                               ccccGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCt                                                                       <  1:2959554/101‑1 (MQ=255)
                               ccccGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCt                                                                       <  1:1433221/101‑1 (MQ=255)
                               ccccGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCt                                                                       <  2:3211393/101‑1 (MQ=255)
                                 ccGGGATATTT.GGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTG.GCCG.AAGGCCAAGCGGGGCGCAGCGCCCCCttt                                                                     >  2:754805/1‑99 (MQ=18)
                                    ggATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGcccc                                                                  <  2:4579528/101‑1 (MQ=255)
                                    ggATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGcccc                                                                  >  2:4511024/1‑101 (MQ=255)
                                     gATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCg                                                                 >  1:491/1‑101 (MQ=255)
                                      atatTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCgg                                                                >  2:4378238/1‑101 (MQ=255)
                                      atatTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGCCGGGCAAAGAGGGG.GCAGCTTCCCCGGCCCCgg                                                                >  1:1140624/1‑101 (MQ=25)
                                       tatTT.GAGGCAAGATCAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGc                                                               <  2:1877120/101‑1 (MQ=255)
                                        atTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGGATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCt                                                              >  1:4350405/1‑101 (MQ=255)
                                        atTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCt                                                              >  1:814222/1‑101 (MQ=255)
                                         ttt.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTg                                                             <  2:4186130/101‑1 (MQ=255)
                                          tt.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTga                                                            >  1:4616179/1‑101 (MQ=255)
                                           t.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTgag                                                           >  1:3422593/1‑101 (MQ=255)
                                           t.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTgag                                                           >  2:3299602/1‑101 (MQ=255)
                                           t.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTgag                                                           <  2:3017445/101‑1 (MQ=255)
                                             gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTgagg                                                          >  2:591324/1‑101 (MQ=255)
                                             gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTgagg                                                          <  2:2900780/101‑1 (MQ=255)
                                               ggCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTgaggga                                                        <  2:2363570/101‑1 (MQ=255)
                                                gCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTgagggag                                                       <  1:2727203/101‑1 (MQ=255)
                                                   aGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGTCg                                                    >  1:3727944/1‑101 (MQ=255)
                                                   aGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCg                                                    >  2:2768646/1‑101 (MQ=255)
                                                     aTGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGa                                                  <  1:142613/101‑1 (MQ=255)
                                                      tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa                                                 >  1:2305734/1‑101 (MQ=255)
                                                       gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc                                                >  1:3655542/1‑101 (MQ=255)
                                                         aaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTg                                              >  1:3973281/1‑101 (MQ=255)
                                                           cacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGa                                            <  1:1672176/101‑1 (MQ=255)
                                                             cGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaaa                                          >  1:2550916/1‑101 (MQ=255)
                                                             cGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaaa                                          <  1:276783/101‑1 (MQ=255)
                                                                  cTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg                                     >  1:3325181/1‑101 (MQ=255)
                                                                   tttCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGa                                    >  1:3866811/1‑101 (MQ=255)
                                                                    ttCCTCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGC.GTAGCTCCCTCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGaa                                   <  2:2275453/101‑1 (MQ=38)
                                                                    ttCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGagatcggaag                                   >  2:148874/1‑92 (MQ=255)
                                                                    ttCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGaa                                   >  2:2030474/1‑101 (MQ=255)
                                                                     tCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg                                  >  2:4546909/1‑101 (MQ=255)
                                                                     tCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg                                  <  1:1541022/101‑1 (MQ=255)
                                                                       aTCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAc                                >  1:3528387/1‑101 (MQ=255)
                                                                         cTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc                              >  2:3504013/1‑101 (MQ=255)
                                                                             ccccAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCt                          <  1:1001811/101‑1 (MQ=255)
                                                                              cccAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTg                         <  2:4036686/101‑1 (MQ=255)
                                                                                cAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGAt                       <  2:3131618/101‑1 (MQ=255)
                                                                                cAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGAt                       >  1:783209/1‑101 (MQ=255)
                                                                                 caaatcgcgggggggTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCCCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGAt                       <  1:4511274/91‑1 (MQ=255)
                                                                                 aaaTATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                      >  1:3180795/1‑101 (MQ=255)
                                                                                 aaaTATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                      >  2:4249134/1‑101 (MQ=255)
                                                                                 aaaTATCCA.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                      <  1:4102716/101‑1 (MQ=255)
                                                                                  aaTATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg                     >  1:2146819/1‑101 (MQ=255)
                                                                                   atatCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGa                    <  1:2896590/101‑1 (MQ=255)
                                                                                   atatCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGa                    >  2:3955886/1‑101 (MQ=255)
                                                                                    tatCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                   >  2:1045339/1‑101 (MQ=255)
                                                                                    tatCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                   >  2:3744686/1‑101 (MQ=255)
                                                                                     atCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAag                  <  1:4152447/101‑1 (MQ=255)
                                                                                       gggggggggTGATTTG.GCCGGGAGGAAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAaga                 <  2:2550764/97‑1 (MQ=255)
                                                                                       cgcggggggGGAATTTGGCCGAGAGAAAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAag                  <  1:3118596/97‑1 (MQ=255)
                                                                                        cc.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAgg               >  2:1816993/1‑101 (MQ=255)
                                                                                           gggggTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc             <  2:2394932/101‑1 (MQ=255)
                                                                                           gggggTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc             >  1:1227201/1‑101 (MQ=255)
                                                                                           gggggTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc             >  1:565558/1‑101 (MQ=255)
                                                                                                 gAATTT.GCCGTCAGGCAAAGCGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcgcgcg       >  1:374311/1‑101 (MQ=255)
                                                                                                  aaTTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGa      >  2:3655832/1‑101 (MQ=255)
                                                                                                  aaTTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGa      >  2:3327815/1‑101 (MQ=255)
                                                                                                   aTTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGAt     >  2:4626313/1‑101 (MQ=255)
                                                                                                   aTTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGAt     >  1:3800783/1‑101 (MQ=255)
                                                                                                    ttt.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc    >  1:4739829/1‑101 (MQ=255)
                                                                                                     tt.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCg   <  2:267967/101‑1 (MQ=255)
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CTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTT.GCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGG  >  NC_014034/2950654‑2950854

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG