Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,760 C→A 4.2% intergenic (+103/‑57) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7600C→A 4.2% 20.3 ‑0.2 ‑1.4294intergenic (+103/‑57)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (7/6):  ref base (164/117):  total (171/123)
Fisher's exact test strand distribution p-value = 7.79e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.62e-02

Only 100 of 321 total aligned reads displayed.

CAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTGC  >  NC_014034/2950660‑2950860
                                                                                                     |                                                                                                     
caagcCGGGGGCTCTGCCCCCGGACCCCCGGGGTATTTTGGGCAAAAAGAAAACCCCCCCCTTTATCTTCCCCCCAAAATCCC.GGGGGGGGATTTGCCGTc                                                                                                       >  1:3383289/1‑101 (MQ=9)
 aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                                                                      <  2:2469831/101‑1 (MQ=255)
 aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                                                                      >  2:276749/1‑101 (MQ=255)
  agcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAg                                                                                                     <  2:4211470/101‑1 (MQ=255)
   gcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAgg                                                                                                    <  2:3218277/101‑1 (MQ=255)
   gcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAgg                                                                                                    <  2:909018/101‑1 (MQ=255)
    ccGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGc                                                                                                   <  2:3179684/101‑1 (MQ=255)
     cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                                                                                  <  2:4601102/101‑1 (MQ=255)
     cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCa                                                                                                  >  1:1745077/1‑101 (MQ=255)
      gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaa                                                                                                 >  2:2355644/1‑101 (MQ=255)
      gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaa                                                                                                 >  1:4186376/1‑101 (MQ=255)
        gggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAag                                                                                               <  1:2642116/101‑1 (MQ=255)
         ggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAaga                                                                                              <  2:1619554/101‑1 (MQ=255)
          gCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAagag                                                                                             <  1:1992121/101‑1 (MQ=255)
             ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAgggg                                                                                          >  2:1767295/1‑101 (MQ=255)
              tGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAgggg.g                                                                                        >  1:1681552/1‑101 (MQ=255)
              tGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAgggg.g                                                                                        >  1:3788241/1‑101 (MQ=255)
              tGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAgggg.g                                                                                        >  2:1804674/1‑101 (MQ=255)
              cttcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAgggg.gg                                                                                       >  2:2067505/10‑100 (MQ=25)
               gCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCAAAGAgggg.gg                                                                                       >  2:4065671/1‑100 (MQ=255)
                tcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAgggg.ggca                                                                                     <  2:3665917/94‑4 (MQ=25)
                 ccccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAg                                                                                     <  1:2420912/101‑1 (MQ=255)
                  cccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGc                                                                                    >  2:509551/1‑101 (MQ=255)
                    cGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTc                                                                                  >  1:1864579/1‑101 (MQ=255)
                    cGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTc                                                                                  >  2:439562/1‑101 (MQ=255)
                     ggACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTcc                                                                                 <  2:1546001/101‑1 (MQ=255)
                     ggACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTcc                                                                                 <  2:2891914/101‑1 (MQ=255)
                     ggACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTcc                                                                                 >  1:2280564/1‑101 (MQ=255)
                     ctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGAGGGG.GCAGCGccc                                                                                >  2:2696761/4‑101 (MQ=2)
                      tccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGAGGGG.GCAGCGcccc                                                                               <  1:3740144/99‑1 (MQ=2)
                       aCCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTcccc                                                                               >  1:4271625/1‑101 (MQ=255)
                         ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCt                                                                             <  1:945291/101‑1 (MQ=255)
                         ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCt                                                                             <  1:2959554/101‑1 (MQ=255)
                           ccGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCG.AAGGCCAAGCGGGGCGCAGCGCCCCCttt                                                                           >  2:754805/1‑99 (MQ=18)
                             gggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGccc                                                                         <  2:125936/101‑1 (MQ=255)
                              ggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGcccc                                                                        >  2:4511024/1‑101 (MQ=255)
                               gATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCg                                                                       >  2:3798779/1‑101 (MQ=255)
                                atatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCgg                                                                      >  2:2635041/1‑101 (MQ=255)
                                atatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCgg                                                                      >  2:4378238/1‑101 (MQ=255)
                                atatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGCCGGGCAAAGAGGGG.GCAGCTTCCCCGGCCCCgg                                                                      >  1:1140624/1‑101 (MQ=25)
                                 tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGc                                                                     <  2:2771842/101‑1 (MQ=255)
                                  atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCt                                                                    >  1:56973/1‑101 (MQ=255)
                                  atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCt                                                                    >  1:814222/1‑101 (MQ=255)
                                    ttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTga                                                                  <  2:2188631/101‑1 (MQ=255)
                                    ttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTga                                                                  >  1:4616179/1‑101 (MQ=255)
                                    ttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTga                                                                  <  2:1595881/101‑1 (MQ=255)
                                     tGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTgag                                                                 <  2:3017445/101‑1 (MQ=255)
                                     tGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTgag                                                                 >  2:3299602/1‑101 (MQ=255)
                                      gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTgagg                                                                <  2:2900780/101‑1 (MQ=255)
                                      gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTgagg                                                                >  2:591324/1‑101 (MQ=255)
                                      gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTgagg                                                                >  2:1095417/1‑101 (MQ=255)
                                      gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTACCCCTGCCCCGGCTgagg                                                                >  1:52034/1‑101 (MQ=255)
                                       aGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTgaggg                                                               >  1:3680184/1‑101 (MQ=255)
                                         gCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTgagggag                                                             >  1:2362729/1‑101 (MQ=255)
                                               tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa                                                       <  1:3929822/101‑1 (MQ=255)
                                                gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc                                                      >  1:3092623/1‑101 (MQ=255)
                                                gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc                                                      <  2:2382042/101‑1 (MQ=255)
                                                 aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAcg                                                     >  1:2767068/1‑100 (MQ=255)
                                                 aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt                                                     >  2:3118396/1‑101 (MQ=255)
                                                 aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt                                                     >  1:4743800/1‑101 (MQ=255)
                                                     acGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaa                                                 <  1:3814400/101‑1 (MQ=255)
                                                      cGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaaa                                                >  1:2550916/1‑101 (MQ=255)
                                                         cccTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCt                                             <  1:4514302/101‑1 (MQ=255)
                                                           cTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg                                           >  1:3325181/1‑101 (MQ=255)
                                                            tttCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGa                                          <  1:1260357/101‑1 (MQ=255)
                                                             ttCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGaa                                         >  2:3652144/1‑101 (MQ=255)
                                                             ttCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGaa                                         >  1:3151733/1‑101 (MQ=255)
                                                               cATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGa                                       >  2:1850804/1‑101 (MQ=255)
                                                               cATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGa                                       >  1:413088/1‑101 (MQ=255)
                                                                aTCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAc                                      >  1:3528387/1‑101 (MQ=255)
                                                                  cTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc                                    >  2:3504013/1‑101 (MQ=255)
                                                                   ttGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCg                                   >  2:1136435/1‑101 (MQ=255)
                                                                      ccccAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCt                                <  1:1001811/101‑1 (MQ=255)
                                                                       cccAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTg                               <  2:4271378/101‑1 (MQ=255)
                                                                         cAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGAt                             >  1:783209/1‑101 (MQ=255)
                                                                          aaaTATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                            >  1:1502308/1‑101 (MQ=255)
                                                                           aaTATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccagatcggaag                           >  1:1612850/1‑91 (MQ=255)
                                                                           aaTATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg                           >  1:2146819/1‑101 (MQ=255)
                                                                           aaTATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg                           >  1:1526427/1‑101 (MQ=255)
                                                                           aaTATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg                           >  2:1349904/1‑101 (MQ=255)
                                                                            atatCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGa                          <  1:2896590/101‑1 (MQ=255)
                                                                             tatCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                         >  2:1045339/1‑101 (MQ=255)
                                                                              attcc.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAag                        >  1:2707183/4‑101 (MQ=255)
                                                                               tCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAaga                       <  2:4527292/101‑1 (MQ=255)
                                                                                  c.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGc                    >  2:1456889/1‑101 (MQ=255)
                                                                                    gggggTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc                   >  2:4014051/1‑101 (MQ=255)
                                                                                    gggggTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc                   <  2:2305601/101‑1 (MQ=255)
                                                                                          gAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcgcgcg             >  1:1429856/1‑101 (MQ=255)
                                                                                           aaTTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGa            >  2:3327815/1‑101 (MQ=255)
                                                                                             tttGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc          >  2:4616685/1‑101 (MQ=255)
                                                                                              ttGCCGTCAGGCAAAGAGGGG.GCCGCTCCCCCTGTCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGTCCCGCCTGATCGAAGAGGCCCGCGCGATCg         <  1:4074612/101‑1 (MQ=255)
                                                                                              ttGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCg         >  2:4576830/1‑101 (MQ=255)
                                                                                                 ccGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCt      >  1:4568585/1‑101 (MQ=255)
                                                                                                  cGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCtt     <  1:3048833/101‑1 (MQ=255)
                                                                                                   gTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGcaga    >  1:1559222/1‑98 (MQ=255)
                                                                                                   gTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCttt    <  1:3245032/101‑1 (MQ=255)
                                                                                                    tCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTg   <  2:1894481/101‑1 (MQ=255)
                                                                                                     cAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTGc  >  2:4151434/1‑101 (MQ=255)
                                                                                                     cAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTGc  >  1:4433758/1‑101 (MQ=255)
                                                                                                     cAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTGc  >  1:3400684/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
CAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTGC  >  NC_014034/2950660‑2950860

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG