Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,953,208 C→A 100% intergenic (+97/+281) RCAP_rcc02764 → / ← ilvE1 hypothetical protein/branched‑chain‑amino‑acid transaminase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,953,2080C→A100.0% 521.8 133intergenic (+97/+281)RCAP_rcc02764/ilvE1hypothetical protein/branched‑chain‑amino‑acid transaminase
Reads supporting (aligned to +/- strand):  new base (36/97):  ref base (0/0):  total (36/97)

Only 100 of 195 total aligned reads displayed.

GAACCGGACCCCCTGAAACCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGCGCAGACGGCCCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCA  >  NC_014034/2953110‑2953306
                                                                                                  |                                                                                                   
gAACCGGACCCCCTGAAACCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTgagc                                                                                                   <  1:4184154/101‑1 (MQ=255)
 aaCCGGACCCCCTGAAACCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCa                                                                                                  <  1:2936513/101‑1 (MQ=255)
 aaCCGGACCCCCTGAAACCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAAAACAAGGGGGGCCGGGGGCa                                                                                                  >  2:284009/1‑101 (MQ=255)
   ccGGACCCCCTGAAACCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGa                                                                                                <  1:4317533/101‑1 (MQ=255)
    cGGACCCCCTGAAACCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGGTGAAAAAGAACAAAGCGGGGCGCGGGGAGAc                                                                                               >  1:4463378/1‑101 (MQ=255)
    cGGACCCCCTGAAACCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGAc                                                                                               <  2:837767/101‑1 (MQ=255)
    cGGACCCCCTGAAACCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGAc                                                                                               <  2:4624874/101‑1 (MQ=255)
    cGGACCCCCTGAAACCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGAc                                                                                               <  2:4410107/101‑1 (MQ=255)
    cGGACCCCCTGAAACCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAAACCAAGGCGGGGCGGGGGCAGAc                                                                                               >  1:3283196/1‑101 (MQ=255)
    cGGACCCCCTGAAACCCGGGGGCCGCAACGGTGACGGGAATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGAc                                                                                               <  1:3880250/101‑1 (MQ=255)
     ggACCCCCTGAAACCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAAGCGGGCCGTGAGGAGaag                                                                                              >  2:2644025/1‑99 (MQ=255)
         ccccTGAAACCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAAGCGGGCCGGGGGCAGAAGGccc                                                                                          >  1:2010745/1‑101 (MQ=255)
         ccccTGAAACCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGGAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGGccc                                                                                          <  2:1109353/101‑1 (MQ=255)
          cccTGAAACCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGGcccg                                                                                         <  1:1574428/101‑2 (MQ=255)
           ccTGAAACCCGGGGGCCGCATCGGGGACGGGCATGCCGCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGGcccgc                                                                                        <  2:1312244/101‑3 (MQ=255)
             tGAACCCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGGTGAAAAAAAACAAAGAGGGGCGCGAGAAGACGGcccgccc                                                                                      >  2:4016249/1‑97 (MQ=255)
               aaaCCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGGTGAAAAAGACCAAGGGGGGGCGGGGGCAGACGGGCCGGCCtg                                                                                    >  1:637466/1‑100 (MQ=255)
               aaaCCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTg                                                                                   <  2:2261736/101‑1 (MQ=255)
                aaCCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTgg                                                                                  <  2:2518044/101‑1 (MQ=255)
                 aCCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGGCGGGAGCAGACGGcccgccctggg                                                                                  >  2:3621061/1‑93 (MQ=255)
                 aCCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.t                                                                                <  1:2644179/101‑1 (MQ=255)
                   ccGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCt                                                                              <  1:711809/101‑1 (MQ=255)
                       gggCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTc                                                                          <  1:1398811/101‑1 (MQ=255)
                       gggCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTc                                                                          <  1:3604040/101‑1 (MQ=255)
                       gggCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTc                                                                          <  1:842454/101‑1 (MQ=255)
                         gCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGGTGAAAAAGAACAAAGCGGGGCGGGGGCAGACGG.CCCGCCCTGG.TCTTTTTgg                                                                        >  1:3281478/1‑101 (MQ=255)
                           cGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGGTGAAAAAAACCAAAGGGGGCCGGGGGGAGGCGG.GCCGCCCTGG.TGtttttcggg                                                                      >  2:3444800/1‑96 (MQ=255)
                           cGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATTAAAAAAACCAAAAAGGGCCGTGAGCAGACAGCCCCCCCTGGG.TCtttctggg                                                                       >  2:2157214/1‑96 (MQ=255)
                             cAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGgc                                                                    <  2:94803/101‑1 (MQ=255)
                               aCGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGgcgc                                                                  <  1:120872/101‑1 (MQ=255)
                                cGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCa                                                                 <  2:4115500/101‑1 (MQ=255)
                                cGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCa                                                                 <  1:3837387/101‑1 (MQ=255)
                                 ggTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAAACCAAGGGGGGCCGGGGGCAGACGGGCCGCCCTGGGCTTTTTTGGGGGgcgt                                                                  >  1:1728150/1‑100 (MQ=255)
                                 ggTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGAGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCAt                                                                <  1:924294/101‑1 (MQ=255)
                                  gTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCAtt                                                               <  2:2578283/101‑1 (MQ=255)
                                  gTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAAACCAAGGCGGGCCGTGGGCAGACGG.CCCGCCCTGG.TCTTTTTGGGGGGGCCtt                                                               >  2:4170686/1‑101 (MQ=255)
                                  gTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAAACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCCTGG.TCTTTTCGGGGGGGCAtt                                                               >  1:1547195/1‑101 (MQ=255)
                                   tGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATtc                                                              <  2:1546594/101‑1 (MQ=255)
                                    gACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCCTGG.TCTTTTCGGGGGGGCATtct                                                             >  2:1749538/1‑101 (MQ=39)
                                     aCGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGGTGAAAAAAAACAAAGCGGGGCGTGAGCAGACGGCCCGCCCTTGG..CTTTTTTGGGGGGCATtctc                                                            >  2:3343019/1‑101 (MQ=255)
                                      cGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTTGGGGGCGCATTctct                                                           >  1:707330/1‑101 (MQ=255)
                                      cGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTctct                                                           <  2:637402/101‑1 (MQ=255)
                                        ggCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGa                                                         <  2:4200464/101‑1 (MQ=255)
                                         ggaTGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGaa                                                        <  1:1004251/99‑1 (MQ=255)
                                         gCATGGCTCCGGCGTGCCACCGGAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGaa                                                        <  2:3461191/101‑1 (MQ=38)
                                          cATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGaaa                                                       <  2:3904103/101‑1 (MQ=255)
                                           aTGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAg                                                      <  1:58117/101‑1 (MQ=255)
                                            tGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAAACCAAAGCGGGGCGTGGGCAGACCG.CCCGCCCTGG.TCTTTTTCGGGGGGCCTTCTCTCaaaag                                                     >  1:1820795/1‑99 (MQ=255)
                                             gTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAgcg                                                    <  1:2606713/101‑1 (MQ=255)
                                               ctcCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGGGAGCAGACGG.CCCGCCCTGG.TCTTTTCGGGGGCGCCTTTTCTTAAAgcggc                                                  >  2:104004/1‑99 (MQ=255)
                                                tcCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCt                                                 <  2:2010628/101‑1 (MQ=255)
                                                 ccGGCGTGCCACGGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTg                                                <  2:1703610/101‑1 (MQ=255)
                                                 ccGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGCCGCATTCTCTGAAAGCGCCTg                                                <  1:3495878/101‑1 (MQ=255)
                                                    gCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.GCTTTTCGGGGGGGCATTCTCTGAAAGCGCCTGACt                                             >  1:1310901/1‑101 (MQ=255)
                                                     cGTGCCACCGTAGCGGCGGGAAGAAAAAAACCAAGGCGGGCCCGGAGCAGACGG.CCCGCCTTGG.GCTTTTTGGGGGGGCCTTCTCTGAAAGCGCCCGACtt                                            >  2:1467920/1‑101 (MQ=255)
                                                      gTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGGGCAGACGG.CCCGCCTTGG.GCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTg                                           >  1:2550449/1‑101 (MQ=255)
                                                      gTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTg                                           <  2:2993423/101‑1 (MQ=255)
                                                       tGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGGCGTGAGCAGACGG.CCCGCCTTGG.GCTTTTCGGGGGGGCATTTTCTGAAAGCGCCCTACTTgg                                          >  2:809911/1‑101 (MQ=37)
                                                        gCCACCGTAGCGGCGGGATGAAAAAAACCAAGGCGGGCCGTGGGGAGACGG.CCCGCCTTGG.TCTTTTCGGGGGGGCAGTCTCTGAAAGCGCCTGACCTGGc                                         >  1:4151940/1‑101 (MQ=255)
                                                         ccACCGGAGGGGGGGGATTAAAAAGGCCAAGGGGGGCCGTTAGGAGACGG.CCCGCCTTTG.GCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGcc                                        <  1:2439359/101‑1 (MQ=255)
                                                          cACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCcg                                       <  2:171512/101‑1 (MQ=255)
                                                            ccGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCcgcg                                     <  1:3048072/101‑1 (MQ=255)
                                                             cGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGGGGGCAGACGG.GCCGCCCTGG.GCTTTTCGGGGGCGGCTTCTCTGGAAGCGCCTGACTTGGCCGCgg                                    >  1:4584323/1‑101 (MQ=255)
                                                              gTAGCGGCGGGATGAAAAAGACCAAAGCGGGGCGCGAGCAGACGG.GCCGCCCTGG.TCCTTTTGGGGGCGGCGTCTCTGAAAGGGGCTAAACTGTCCGCGGc                                   >  2:93358/1‑101 (MQ=255)
                                                               tAGCGGCGGGGTGAAAAAGACCAAGGCGGGGCGTGAGCAAACGG.CCCGCCTTTG.TCTTTTCGGGGGCGCGTTCTCTGAAAGCGCCTGACTTGGCCGCGGcc                                  >  2:4729256/1‑101 (MQ=37)
                                                               tAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTTTCTGAAAGCGCCTGACTTGGGCGCGGcc                                  >  2:4561117/1‑101 (MQ=38)
                                                               tAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGcc                                  <  1:3932572/101‑1 (MQ=255)
                                                                aGCGGCGGGATGAAAAAAACCAAGGCGGGGCGGGAGCAGACGG.GCCGCCTTGG.GCTTTTTGGGGGGGCATTTTCTGAAAGCGCCTGACTTTGGCGCGGCCg                                 >  2:1606354/1‑101 (MQ=255)
                                                                   ggcggGATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCgggg                              >  1:3398444/1‑99 (MQ=255)
                                                                     cggGATGAAAAAGACCAAGGCGGGCCGTGAGCCGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGACAGCGCCTGACTTGGCCGCCGCCGGTGAc                            >  1:1258556/1‑101 (MQ=38)
                                                                      gggATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGAcg                           >  1:2528791/1‑101 (MQ=255)
                                                                      gggATAAAAAAGACCAGGGCGGGCCGGGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGAcg                           <  1:2081005/101‑1 (MQ=255)
                                                                       ggATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGAcgc                          >  1:2742452/1‑101 (MQ=255)
                                                                        gATGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGAcgcg                         >  1:2792676/1‑101 (MQ=255)
                                                                         aTGAAAAAGCCCAAGGGGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCgg                        <  2:2312810/101‑1 (MQ=39)
                                                                         aTGAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCgg                        >  1:1298136/1‑101 (MQ=255)
                                                                           gAAAAAGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGcc                      >  2:2412423/1‑101 (MQ=255)
                                                                             aaaaGACCAAGGGGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAt                    <  1:1111580/101‑1 (MQ=255)
                                                                             aaaaGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAt                    <  1:3052951/101‑1 (MQ=255)
                                                                             aaaaGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAt                    <  1:3538934/101‑1 (MQ=255)
                                                                             aaaaGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAt                    <  2:3389251/101‑1 (MQ=255)
                                                                             aaaaGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAt                    <  2:886786/101‑1 (MQ=255)
                                                                               aaGACCAGGGGGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGc                  <  1:2861605/101‑1 (MQ=38)
                                                                               aaGACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGc                  <  1:1499790/101‑1 (MQ=255)
                                                                                 gACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGTCGCGGCCGGTGCCGCGGCCATGCga                >  2:295727/1‑101 (MQ=255)
                                                                                 gACCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCga                <  2:2953588/101‑1 (MQ=255)
                                                                                  aCCAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCgag               <  2:3282995/101‑1 (MQ=255)
                                                                                   ccAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCgaga              >  1:4706657/1‑101 (MQ=255)
                                                                                   ccAAGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGGGACGCGGCCATGCgaga              >  1:1711779/1‑101 (MQ=255)
                                                                                     aaGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACa            >  2:3742293/1‑101 (MQ=255)
                                                                                      aGGCGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACAt           >  1:226018/1‑101 (MQ=255)
                                                                                         cGGGCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTg        >  1:4504460/1‑101 (MQ=255)
                                                                                          gggCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGc       <  2:4463128/101‑1 (MQ=255)
                                                                                          gggCCGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGc       >  1:2239772/1‑101 (MQ=255)
                                                                                              cGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTc   >  2:191063/1‑101 (MQ=255)
                                                                                              cGTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTc   <  2:2676142/101‑1 (MQ=255)
                                                                                               gTGAGCAGACGG.CCCGCCTTTG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTca  <  1:3357960/101‑1 (MQ=255)
                                                                                               gTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTca  >  1:2982333/1‑101 (MQ=255)
                                                                                               gTGAGCAGACGG.CCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTca  >  1:4172483/1‑101 (MQ=255)
                                                                                               gTGAGCAGACGG.CCCACCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTca  >  1:4003851/1‑101 (MQ=255)
                                                                                                  |                                                                                                   
GAACCGGACCCCCTGAAACCCGGGGGCCGCAACGGTGACGGGCATGTCTCCGGCGTGCCACCGTAGCGGCGGGATGAAAAAGACCAAGGCGGGCCGTGCGCAGACGGCCCCGCCTTGG.TCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCA  >  NC_014034/2953110‑2953306

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG