Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,959,195 T→C 100% E74E (GAA→GAG RCAP_rcc02771 ← TetR family transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,959,1950T→C100.0% 718.0 187E74E (GAA→GAGRCAP_rcc02771TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (101/86):  ref base (0/0):  total (101/86)

Only 100 of 189 total aligned reads displayed.

GCCCCAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCTTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGTGTCGCCTTGGACACCCCC  >  NC_014034/2959098‑2959291
                                                                                                 |                                                                                                
gCCCCAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCa                                                                                               <  1:4563188/101‑1 (MQ=255)
 ccccAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCAt                                                                                              <  1:4454132/101‑1 (MQ=255)
 ccccAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCAt                                                                                              <  2:4206090/101‑1 (MQ=255)
  cccAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATc                                                                                             <  2:2588948/101‑1 (MQ=255)
   ccAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCg                                                                                            <  2:852526/101‑1 (MQ=255)
   ccAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCg                                                                                            >  2:4301997/1‑101 (MQ=255)
   ccAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCg                                                                                            >  1:1758104/1‑101 (MQ=255)
       cggcgAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCt                                                                                        >  1:4333092/1‑101 (MQ=255)
       cggcgAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCt                                                                                        <  1:3252145/101‑1 (MQ=255)
        ggcgAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTg                                                                                       <  2:1837116/101‑1 (MQ=255)
        ggcgAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTg                                                                                       <  1:2595525/101‑1 (MQ=255)
          cgAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGgc                                                                                     >  2:2625638/1‑101 (MQ=255)
           gAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGgcg                                                                                    >  2:449600/1‑101 (MQ=255)
            aGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGgcgc                                                                                   >  2:2333539/1‑101 (MQ=255)
              gTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTg                                                                                 <  1:3771115/101‑1 (MQ=255)
              gTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCACCTCATCGGCCTGGCGCTg                                                                                 >  1:4522324/1‑101 (MQ=255)
               tGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTgg                                                                                >  2:246083/1‑101 (MQ=255)
               tGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTgg                                                                                >  1:4663453/1‑101 (MQ=255)
               tGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTgg                                                                                <  2:1406920/101‑1 (MQ=255)
                gATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGc                                                                               >  1:2939632/1‑101 (MQ=255)
                 aTGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCa                                                                              >  2:682941/1‑101 (MQ=255)
                 aTGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCa                                                                              >  2:3825642/1‑101 (MQ=255)
                 aTGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCa                                                                              <  1:3295278/101‑1 (MQ=255)
                   gTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCAtt                                                                            >  1:2283392/1‑101 (MQ=255)
                   gTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCAtt                                                                            <  1:2351201/101‑1 (MQ=255)
                    tAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTc                                                                           >  2:2742387/1‑101 (MQ=255)
                    tAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTc                                                                           >  1:3646795/1‑101 (MQ=255)
                        cGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGccc                                                                       <  1:3126638/101‑1 (MQ=255)
                        cGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGccc                                                                       <  1:2544477/101‑1 (MQ=255)
                          aTCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGt                                                                     <  1:3293670/101‑1 (MQ=255)
                           tCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTc                                                                    >  2:1719001/1‑101 (MQ=255)
                             aGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCCGCCTGGCGCTGGCACTCGCCCGTcgc                                                                  >  2:4673722/1‑101 (MQ=255)
                              gCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTcgcg                                                                 >  2:3664930/1‑101 (MQ=255)
                              gCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTcgcg                                                                 >  2:3780908/1‑101 (MQ=255)
                              gCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTcgcg                                                                 <  2:3380651/101‑1 (MQ=255)
                              gCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTcgcg                                                                 <  1:1007818/101‑1 (MQ=255)
                                 tgtgGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATc                                                              >  1:1631622/1‑101 (MQ=255)
                                  gtgGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCg                                                             >  2:161511/1‑101 (MQ=255)
                                  gtgGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCg                                                             >  1:4665540/1‑101 (MQ=255)
                                   tgGCCCGCATAGCGCAGCACCTCGGGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGGATTCGGCCGGCGCGATCGc                                                            >  2:1029016/1‑101 (MQ=255)
                                   tgGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGc                                                            >  2:824930/1‑101 (MQ=255)
                                   tgGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGc                                                            <  1:3978204/101‑1 (MQ=255)
                                   tgGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGc                                                            >  1:625733/1‑101 (MQ=255)
                                    ggCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGcc                                                           <  2:3872720/101‑1 (MQ=255)
                                    ggCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGcc                                                           >  1:1346562/1‑101 (MQ=255)
                                    ggCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGcc                                                           >  1:1050687/1‑101 (MQ=255)
                                    ggCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATAGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGcc                                                           >  2:4298814/1‑101 (MQ=255)
                                      cccGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCAt                                                         >  1:3517368/1‑101 (MQ=255)
                                      cccGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCAt                                                         <  1:4172560/101‑1 (MQ=255)
                                         gCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGaa                                                      >  1:24867/1‑101 (MQ=255)
                                         gCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGaa                                                      >  2:2013106/1‑101 (MQ=255)
                                         gCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGaa                                                      >  1:832265/1‑101 (MQ=255)
                                           aTAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAAca                                                    <  1:3814418/101‑1 (MQ=255)
                                           aTAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAAca                                                    <  1:1795313/101‑1 (MQ=255)
                                            tAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAAcag                                                   >  2:708054/1‑101 (MQ=255)
                                             aGNGCNGCCCCTCGCGCNCCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCTTCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAAcagc                                                  <  2:1146184/101‑1 (MQ=255)
                                             aGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATAGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAAcagc                                                  <  1:4299061/101‑1 (MQ=255)
                                              gcgcAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAAcagca                                                 <  2:3244024/101‑1 (MQ=255)
                                                gcagcaCCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGATCagcagc                                               >  1:805632/1‑101 (MQ=255)
                                                gcagcaCCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACagcagc                                               >  1:1113346/1‑101 (MQ=255)
                                                gcagcaCCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACagcagc                                               >  1:1959338/1‑101 (MQ=255)
                                                gcagcaCCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACagcagc                                               >  1:3158449/1‑101 (MQ=255)
                                                 cagcaCCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTTGCATTCGCCCGTCGCGCTCGCCATGAACAGAAGcc                                              >  1:1914889/1‑101 (MQ=255)
                                                     aCCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCtt                                          >  2:4444991/1‑101 (MQ=255)
                                                        tCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCTCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTc                                       <  2:2714179/101‑1 (MQ=255)
                                                         cgcgCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTcc                                      <  1:3629640/101‑1 (MQ=255)
                                                          gcgcACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCCGGCATTCGCCCGTCGCGAGCGCCATGCACCGCAGCCGCTTGTCCg                                     >  2:1004748/1‑101 (MQ=255)
                                                           cgcACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCgg                                    <  1:3600551/101‑1 (MQ=255)
                                                               ccGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGaaa                                >  1:2240320/1‑101 (MQ=255)
                                                                cGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAAt                               <  1:2771101/101‑1 (MQ=255)
                                                                 ggCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATa                              <  2:1511418/101‑1 (MQ=255)
                                                                  gCCTGTCGCCCCCATTGATGAACTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAg                             <  2:1542336/101‑1 (MQ=255)
                                                                   ccTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGc                            <  2:3042253/101‑1 (MQ=255)
                                                                    cTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCt                           >  2:4047264/1‑101 (MQ=255)
                                                                    cTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCt                           <  2:625669/101‑1 (MQ=255)
                                                                    cTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCt                           <  2:215123/101‑1 (MQ=255)
                                                                     tGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTg                          <  1:4376182/101‑1 (MQ=255)
                                                                       tCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTa                        >  2:148769/1‑101 (MQ=255)
                                                                       tCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTa                        <  1:3473351/101‑1 (MQ=255)
                                                                       tCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTa                        <  2:2124709/101‑1 (MQ=255)
                                                                       tCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCAATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTa                        >  2:3276671/1‑101 (MQ=255)
                                                                        cGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTag                       <  1:2536854/101‑1 (MQ=255)
                                                                          cccGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTagag                     >  1:485330/1‑101 (MQ=255)
                                                                          cccGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTagag                     <  1:670759/101‑1 (MQ=255)
                                                                           ccGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTagagc                    <  2:1056883/101‑2 (MQ=255)
                                                                            cGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTagagcg                   <  1:735877/101‑3 (MQ=255)
                                                                             gCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAgcgt                  >  1:3351387/1‑101 (MQ=255)
                                                                              cATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTc                 >  2:521902/1‑101 (MQ=255)
                                                                               aTTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCg                >  1:3138228/1‑101 (MQ=255)
                                                                               aTTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCg                <  2:1346470/101‑1 (MQ=255)
                                                                                ttGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGc               <  1:246108/101‑1 (MQ=255)
                                                                                  gatgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCt             >  1:2998854/1‑101 (MQ=255)
                                                                                  gatgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCt             >  2:186781/1‑101 (MQ=255)
                                                                                  gatgaATTCCAGCGCCGCATCGTCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCt             <  2:4522071/101‑1 (MQ=255)
                                                                                   atgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCtt            <  2:1779044/101‑1 (MQ=255)
                                                                                    tgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTg           >  1:1448538/1‑101 (MQ=255)
                                                                                         tCCAGCGCCTCATCGGCTTGGCGCTGGCATTCGCCCGTCGCGGTTGCTATGAAAAGCAGCCGCTTTTCCGGGACATAACTTGAGGGCGTTGCCTTgctgtc      >  2:2955331/1‑96 (MQ=255)
                                                                                           cAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAccc    <  1:1529393/101‑1 (MQ=255)
                                                                                           cAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAccc    <  2:4663197/101‑1 (MQ=255)
                                                                                            aGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAcccc   >  1:2469052/1‑101 (MQ=255)
                                                                                                 |                                                                                                
GCCCCAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCTTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGTGTCGCCTTGGACACCCCC  >  NC_014034/2959098‑2959291

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG