Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,960,499 A→G 100% E123E (GAA→GAG hisP → polar amino acid ABC transporter ATP‑binding protein HisP

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,960,4990A→G100.0% 959.4 249E123E (GAA→GAGhisPpolar amino acid ABC transporter ATP‑binding protein HisP
Reads supporting (aligned to +/- strand):  new base (153/96):  ref base (0/0):  total (153/96)

Only 100 of 254 total aligned reads displayed.

GACCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCT  >  NC_014034/2960401‑2960596
                                                                                                  |                                                                                                 
gACCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGgc                                                                                                 >  1:2327863/1‑101 (MQ=255)
 acccaccgcAGGTAACGCGGTTTCGCACCAACCTGTCGATGGAGTTCCAGCAGCTCAACCTCTGGAGCCACATGACGATGCTGCAAAATGTCATCGAGgcg                                                                                                >  2:948049/7‑101 (MQ=255)
  ccGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGgcgc                                                                                               >  1:2825974/1‑101 (MQ=255)
   cGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGcc                                                                                              <  1:945402/101‑1 (MQ=255)
    gggCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCg                                                                                             <  1:1302811/101‑1 (MQ=255)
    gggCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCg                                                                                             >  1:1020143/1‑101 (MQ=255)
     ggCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTGTGTAGCCACATGACGATCCTGCATAATGTCATGGAGGCGCCgg                                                                                            >  1:328541/1‑101 (MQ=255)
        gcAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGa                                                                                         >  2:412118/1‑101 (MQ=255)
        gcAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGa                                                                                         <  2:606805/101‑1 (MQ=255)
         cAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGAc                                                                                        >  1:2432653/1‑101 (MQ=255)
          aGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACg                                                                                       >  1:4462584/1‑101 (MQ=255)
            gTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGt                                                                                     >  2:2899501/1‑101 (MQ=255)
             tAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGtt                                                                                    <  1:4141790/101‑1 (MQ=255)
             tAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGtt                                                                                    >  2:2137776/1‑101 (MQ=255)
              aaCGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTc                                                                                   <  2:3740557/101‑1 (MQ=255)
                cgcgGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTg                                                                                 >  1:1065145/1‑101 (MQ=255)
                cgcgGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTg                                                                                 <  1:965958/101‑1 (MQ=255)
                   ggTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGc                                                                              <  2:1285113/101‑1 (MQ=255)
                    gTTTCGCACCAACCTGTCGATGGAGTTCCAACAGTTCAACATCTGGAGACACATGACGATCCTGCCAAATGTCATGGCGGCGCCGGTGACGGTTCTGGGcc                                                                             >  2:1837780/1‑101 (MQ=255)
                     tttCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCg                                                                            >  2:1226068/1‑101 (MQ=255)
                      ttCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTCGTCCGc                                                                           >  2:1647952/1‑101 (MQ=255)
                          cACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAAc                                                                       <  1:1501571/101‑1 (MQ=255)
                           aCCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAAcc                                                                      <  1:2658999/101‑1 (MQ=255)
                           aCCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAAcc                                                                      <  2:4737568/101‑1 (MQ=255)
                              aaCCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGc                                                                   >  1:1851798/1‑101 (MQ=255)
                              aaCCTGTCGATGGTATTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGc                                                                   >  2:1998109/1‑101 (MQ=255)
                                ccTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCg                                                                 >  1:4433021/1‑101 (MQ=255)
                                 cTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGa                                                                >  1:1678223/1‑101 (MQ=255)
                                 cTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGa                                                                >  1:461440/1‑101 (MQ=255)
                                 cTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGa                                                                <  2:827985/101‑1 (MQ=255)
                                  tGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGag                                                               >  1:2271027/1‑100 (MQ=255)
                                  tGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGag                                                               >  2:1539128/1‑100 (MQ=255)
                                     cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                            <  1:163513/101‑1 (MQ=255)
                                     cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                            <  1:4676506/101‑1 (MQ=255)
                                     cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                            <  1:141647/101‑1 (MQ=255)
                                     cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                            <  1:1432397/101‑1 (MQ=255)
                                     cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                            >  2:3245402/1‑101 (MQ=255)
                                      gctGGTGTTCCAGCAGTTCACCCTCTGGAGCCCCATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCg                                                           <  1:1108529/99‑1 (MQ=255)
                                      gATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCg                                                           >  1:76843/1‑101 (MQ=255)
                                      gATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCg                                                           >  2:3810380/1‑101 (MQ=255)
                                       aTGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGa                                                          <  1:355595/101‑1 (MQ=255)
                                          gtgtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAcc                                                       >  1:4368117/1‑101 (MQ=255)
                                          gtgtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAcc                                                       <  1:3696574/101‑1 (MQ=255)
                                          gtgtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAcc                                                       >  1:2034207/1‑101 (MQ=255)
                                          gtgtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAcc                                                       >  1:3225090/1‑101 (MQ=255)
                                           tgtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCg                                                      >  1:722093/1‑101 (MQ=255)
                                            gtaccAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGc                                                     >  1:2705288/4‑101 (MQ=255)
                                            gtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGc                                                     <  1:1242906/101‑1 (MQ=255)
                                            gtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGc                                                     >  1:1832338/1‑101 (MQ=255)
                                             ttCCAGCAGTTCAACCTCGGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCt                                                    <  2:1323485/101‑1 (MQ=255)
                                              tCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTc                                                   <  2:348725/101‑1 (MQ=255)
                                               ccAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCg                                                  >  1:1843405/1‑101 (MQ=255)
                                                cagcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGc                                                 >  2:591916/1‑101 (MQ=255)
                                                 agcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGcc                                                >  1:4288258/1‑101 (MQ=255)
                                                   gagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCg                                              <  1:3779519/100‑1 (MQ=255)
                                                   cagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGcccc                                              >  2:2162659/1‑100 (MQ=255)
                                                   cagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCg                                              >  1:3302366/1‑101 (MQ=255)
                                                   cagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCg                                              >  2:2678257/1‑101 (MQ=255)
                                                    agTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGc                                             >  1:3103062/1‑101 (MQ=255)
                                                    agTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGc                                             <  2:39653/101‑1 (MQ=255)
                                                    agTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGc                                             >  1:2863501/1‑101 (MQ=255)
                                                      ttCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAg                                           >  1:650482/1‑101 (MQ=255)
                                                      ttCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAg                                           >  1:321640/1‑101 (MQ=255)
                                                        cacccTCGGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGcc                                         <  2:2960369/98‑1 (MQ=255)
                                                        cAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGcc                                         >  2:4436696/1‑101 (MQ=255)
                                                         aaCCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCt                                        <  1:2232526/101‑1 (MQ=255)
                                                         aaCCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCt                                        >  2:2818577/1‑101 (MQ=255)
                                                          aCCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCtt                                       <  2:3299992/101‑1 (MQ=255)
                                                          aCCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCtt                                       >  2:2478109/1‑101 (MQ=255)
                                                             tctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCGGAGGTCGACCCGCTCGCCCGCAGCCTTCTg                                    <  2:4637255/101‑1 (MQ=255)
                                                              ctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTgg                                   >  1:116023/1‑101 (MQ=255)
                                                                ggAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGcc                                 <  2:3065057/101‑1 (MQ=255)
                                                                 gAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCa                                >  2:2334960/1‑101 (MQ=255)
                                                                    ccACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAgg                             >  2:3081018/1‑101 (MQ=255)
                                                                    ccACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAgg                             >  1:2894432/1‑101 (MQ=255)
                                                                     cacaTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGt                            >  1:863650/1‑101 (MQ=255)
                                                                      acaTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTc                           <  1:1968807/101‑1 (MQ=255)
                                                                      acaTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGCGCCGCAAACCGGCGGAGGTCGAACCGCTCGCCCGCAGCCTTCTGCCCAAGGTc                           >  2:4227261/1‑101 (MQ=255)
                                                                        aTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCgg                         <  1:4223361/101‑1 (MQ=255)
                                                                        aTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCgg                         >  1:4323819/1‑101 (MQ=255)
                                                                          gACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCa                       >  1:1737300/1‑101 (MQ=255)
                                                                          gACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCa                       <  1:479262/101‑1 (MQ=255)
                                                                           aCGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCATCCTTCTGGCCAAGGTCGGCAt                      <  2:2705134/101‑1 (MQ=255)
                                                                           aCGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCAt                      >  2:3022393/1‑101 (MQ=255)
                                                                            cGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATc                     >  2:172108/1‑101 (MQ=255)
                                                                              aTCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAACCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCgg                   >  2:1051072/1‑101 (MQ=255)
                                                                                ccTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCg                 >  1:1562998/1‑101 (MQ=255)
                                                                                ccTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCg                 <  1:2963800/101‑1 (MQ=255)
                                                                                     aaaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaag            >  2:1791029/1‑100 (MQ=255)
                                                                                     aaaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGCCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaag            >  2:3756223/1‑100 (MQ=255)
                                                                                      aaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaagg           >  2:909311/1‑99 (MQ=255)
                                                                                        aTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGcc         >  2:1072447/1‑101 (MQ=255)
                                                                                        aTGTCATGGAGGCGCCGGTGACGGTTCTGGCCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGcc         <  2:3430548/101‑1 (MQ=255)
                                                                                         tGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCg        >  2:1953324/1‑101 (MQ=255)
                                                                                          gTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGa       >  2:2579789/1‑101 (MQ=255)
                                                                                           tCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGAc      >  1:2051772/1‑101 (MQ=255)
                                                                                            cATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACg     >  1:3432364/1‑101 (MQ=255)
                                                                                             aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc    >  2:1120290/1‑101 (MQ=255)
                                                                                              tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGcc   >  1:2646429/1‑101 (MQ=255)
                                                                                               ggAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCt  <  1:3085114/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
GACCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCT  >  NC_014034/2960401‑2960596

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG