Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,960,535 A→G 100% E135E (GAA→GAG hisP → polar amino acid ABC transporter ATP‑binding protein HisP

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,960,5350A→G100.0% 840.4 223E135E (GAA→GAGhisPpolar amino acid ABC transporter ATP‑binding protein HisP
Reads supporting (aligned to +/- strand):  new base (134/89):  ref base (0/0):  total (134/89)

Only 100 of 231 total aligned reads displayed.

CGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCG  >  NC_014034/2960438‑2960632
                                                                                                 |                                                                                                 
cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                                                                >  2:3245402/1‑101 (MQ=255)
cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                                                                <  1:141647/101‑1 (MQ=255)
cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                                                                <  1:1432397/101‑1 (MQ=255)
cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                                                                <  1:4676506/101‑1 (MQ=255)
cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                                                                >  1:2945471/1‑101 (MQ=255)
cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTc                                                                                                <  2:2934917/101‑1 (MQ=255)
 gctGGTGTTCCAGCAGTTCACCCTCTGGAGCCCCATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCg                                                                                               <  1:1108529/99‑1 (MQ=255)
 gATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCg                                                                                               >  2:3414933/1‑101 (MQ=255)
 gATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCg                                                                                               >  1:746510/1‑101 (MQ=255)
  aTGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGa                                                                                              >  1:1778811/1‑101 (MQ=255)
   tGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGaa                                                                                             <  2:1748119/101‑1 (MQ=255)
     gtgtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAcc                                                                                           <  1:3696574/101‑1 (MQ=255)
     gtgtTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAcc                                                                                           >  1:4368117/1‑101 (MQ=255)
        ttCCAGCAGTTCAACCTCGGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCt                                                                                        <  2:1323485/101‑1 (MQ=255)
         tCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTc                                                                                       <  1:1998226/101‑1 (MQ=255)
          ccAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCg                                                                                      >  1:2881664/1‑101 (MQ=255)
           cagcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGc                                                                                     >  2:591916/1‑101 (MQ=255)
            agcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGcc                                                                                    >  1:4288258/1‑101 (MQ=255)
             gcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGccc                                                                                   >  1:3187460/1‑101 (MQ=255)
             gcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGccc                                                                                   <  2:3812543/101‑1 (MQ=255)
             gcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGccc                                                                                   <  2:675957/101‑1 (MQ=255)
             gcagTTCAACCTCTGGAGCCAAATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGccc                                                                                   >  1:2690271/1‑101 (MQ=255)
              gagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCg                                                                                  <  1:3779519/100‑1 (MQ=255)
              cagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCg                                                                                  >  1:2151157/1‑101 (MQ=255)
               agTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGc                                                                                 >  1:3103062/1‑101 (MQ=255)
                gTTCACCCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCa                                                                                <  1:1838889/101‑1 (MQ=255)
                 ttCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAg                                                                               >  1:650482/1‑101 (MQ=255)
                 ttCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAg                                                                               >  2:3470529/1‑101 (MQ=255)
                 ttCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGGCGAACCGCTCGCCCGCAg                                                                               >  2:1288804/1‑101 (MQ=255)
                  tCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGCCGAACCGCTCGCCCGCAGc                                                                              >  2:195236/1‑101 (MQ=255)
                   cAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGcc                                                                             >  2:358654/1‑101 (MQ=255)
                    aaCCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCt                                                                            <  1:2232526/101‑1 (MQ=255)
                     aCCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCtt                                                                           >  1:4516998/1‑101 (MQ=255)
                     aCCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCtt                                                                           <  2:3299992/101‑1 (MQ=255)
                       ctctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCt                                                                         >  1:4319415/1‑101 (MQ=255)
                        tctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCGGAGGTCGACCCGCTCGCCCGCAGCCTTCTg                                                                        <  2:4637255/101‑1 (MQ=255)
                        tctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTg                                                                        >  1:1851657/1‑101 (MQ=255)
                         ctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTgg                                                                       >  1:116023/1‑101 (MQ=255)
                             aGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCaa                                                                   >  1:3223551/1‑101 (MQ=255)
                             aGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCaa                                                                   <  1:231261/101‑1 (MQ=255)
                               ccACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAgg                                                                 >  2:3081018/1‑101 (MQ=255)
                               ccACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAgg                                                                 >  1:2894432/1‑101 (MQ=255)
                                cacaTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGt                                                                >  1:863650/1‑101 (MQ=255)
                                 acaTGACGATCCTGCAAAATGTCATGAAGGCGCCGGTGACGGTTCTGGGCCGCAAACAGCACGAGGACGAACCGATCGCCCGCAGCCTTCTAGCCAAAGTc                                                               >  1:1512963/1‑101 (MQ=255)
                                     gACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCa                                                           <  1:479262/101‑1 (MQ=255)
                                      aCGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCAt                                                          >  2:3022393/1‑101 (MQ=255)
                                       cGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAATCCGGCCGAGGTCGAACCGGTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATc                                                         <  1:948126/101‑1 (MQ=255)
                                        gATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCg                                                        >  2:1707987/1‑101 (MQ=255)
                                         aTCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGCCCAAGGTCGGCATCgg                                                       >  2:3138048/1‑101 (MQ=255)
                                             tGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGAc                                                   >  2:2487215/1‑101 (MQ=255)
                                              gCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACa                                                  <  2:207387/101‑1 (MQ=255)
                                              gCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCAAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACa                                                  >  1:102051/1‑101 (MQ=255)
                                               cAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaa                                                 >  1:995435/1‑101 (MQ=255)
                                                aaaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaag                                                <  2:2201736/101‑2 (MQ=255)
                                                aaaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaag                                                <  1:1648052/101‑2 (MQ=255)
                                                aaaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaag                                                <  2:4580491/101‑2 (MQ=255)
                                                aaaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGCCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaag                                                >  2:3756223/1‑100 (MQ=255)
                                                 aaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaagg                                               >  2:909311/1‑99 (MQ=255)
                                                  aaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGc                                              <  1:3415139/101‑1 (MQ=255)
                                                   aTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGcc                                             >  2:1072447/1‑101 (MQ=255)
                                                     gTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGa                                           >  2:4489752/1‑101 (MQ=255)
                                                     gTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGa                                           >  2:2691175/1‑101 (MQ=255)
                                                      tCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGAc                                          >  1:2051772/1‑101 (MQ=255)
                                                      tCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGAc                                          >  1:2470346/1‑101 (MQ=255)
                                                       cATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACg                                         >  1:1072560/1‑101 (MQ=255)
                                                        aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc                                        >  2:1120290/1‑101 (MQ=255)
                                                         tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGcc                                       >  1:2646429/1‑101 (MQ=255)
                                                          ggAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCt                                      <  1:3085114/101‑1 (MQ=255)
                                                           gcggcgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTg                                     <  2:2339153/98‑1 (MQ=255)
                                                               cgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGccc                                 <  2:4516745/101‑1 (MQ=255)
                                                                gcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCcg                                >  1:2766007/1‑101 (MQ=255)
                                                                 ccGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCcgc                               >  2:1827062/1‑101 (MQ=255)
                                                                      gACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGc                          >  2:775479/1‑101 (MQ=255)
                                                                      gACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGc                          >  2:2718106/1‑101 (MQ=255)
                                                                      gACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGc                          <  2:2646275/101‑1 (MQ=255)
                                                                       aCGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGct                         >  2:2846488/1‑101 (MQ=255)
                                                                         ggTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGctct                       >  2:4140247/1‑101 (MQ=255)
                                                                           ttCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCg                     <  2:20104/101‑1 (MQ=255)
                                                                           ttCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTAGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCg                     >  2:4221274/1‑101 (MQ=255)
                                                                             cTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCggg                   >  2:3988828/1‑101 (MQ=255)
                                                                                gcccGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggcgg                >  2:3608662/3‑101 (MQ=255)
                                                                                    gCAACCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcag            <  1:846743/101‑1 (MQ=255)
                                                                                     cAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagc           <  1:2137897/101‑1 (MQ=255)
                                                                                      aaaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca          >  1:3266466/1‑101 (MQ=255)
                                                                                      aaaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca          >  2:938409/1‑101 (MQ=255)
                                                                                       aaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagcag         >  2:2281869/1‑101 (MQ=255)
                                                                                       aaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagcag         <  1:3582283/101‑1 (MQ=255)
                                                                                         ccGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAgcagca       <  2:1202776/101‑1 (MQ=255)
                                                                                         ccGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAgcagca       <  1:1610846/101‑1 (MQ=255)
                                                                                         ccGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAgcagca       <  2:4380720/101‑1 (MQ=255)
                                                                                         ccGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAgcagca       <  1:3410635/101‑1 (MQ=255)
                                                                                          cGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCaa      >  2:3919733/1‑101 (MQ=255)
                                                                                          cGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCaa      <  1:2545587/101‑1 (MQ=255)
                                                                                           ggCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAc     <  2:288831/101‑1 (MQ=255)
                                                                                             ccGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcgc   >  1:915251/1‑101 (MQ=255)
                                                                                              cGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGNCGGCCAGCAGCAAcgcg  >  2:2356996/1‑101 (MQ=255)
                                                                                              cGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcgcg  >  2:3938907/1‑101 (MQ=255)
                                                                                              cGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcgcg  >  1:403095/1‑101 (MQ=255)
                                                                                              cGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcgcg  <  1:4152691/101‑1 (MQ=255)
                                                                                              cGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGGCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcgcg  >  1:3555589/1‑101 (MQ=255)
                                                                                                 |                                                                                                 
CGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCG  >  NC_014034/2960438‑2960632

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG