Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,960,586 A→G 100% K152K (AAA→AAG hisP → polar amino acid ABC transporter ATP‑binding protein HisP

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,960,5860A→G100.0% 790.3 210K152K (AAA→AAGhisPpolar amino acid ABC transporter ATP‑binding protein HisP
Reads supporting (aligned to +/- strand):  new base (110/100):  ref base (0/0):  total (110/100)

Only 100 of 217 total aligned reads displayed.

AATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTCGACG  >  NC_014034/2960488‑2960683
                                                                                                  |                                                                                                 
aaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGc                                                                                                 <  1:3415139/101‑1 (MQ=255)
 aTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGcc                                                                                                >  2:1072447/1‑101 (MQ=255)
 aTGTCATGGAGGCGCCGGTGACGGTTCTGGCCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGcc                                                                                                <  2:3430548/101‑1 (MQ=255)
  tGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCg                                                                                               >  2:1953324/1‑101 (MQ=255)
  tGTCAGGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCCCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCg                                                                                               <  1:4695521/101‑1 (MQ=255)
   gTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGa                                                                                              >  2:2691175/1‑101 (MQ=255)
    tCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGAc                                                                                             >  1:2051772/1‑101 (MQ=255)
    tCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGAc                                                                                             >  1:989664/1‑101 (MQ=255)
      aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc                                                                                           >  1:1092471/1‑101 (MQ=255)
      aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc                                                                                           >  2:1120290/1‑101 (MQ=255)
       tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGcc                                                                                          >  1:2646429/1‑101 (MQ=255)
       tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGcc                                                                                          >  2:482595/1‑101 (MQ=255)
        ggAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCt                                                                                         <  1:3085114/101‑1 (MQ=255)
         gcggcgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTg                                                                                        <  2:2339153/98‑1 (MQ=255)
          aggcgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTgg                                                                                       <  1:2177113/99‑1 (MQ=255)
          aggcgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTgg                                                                                       <  2:4625306/99‑1 (MQ=255)
           ggcgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGc                                                                                      >  1:2374928/2‑101 (MQ=255)
             cgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGccc                                                                                    <  2:3818283/101‑1 (MQ=255)
               ccGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCcgc                                                                                  >  2:1827062/1‑101 (MQ=255)
                cGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCcgcg                                                                                 <  2:2327730/101‑1 (MQ=255)
                   tGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAg                                                                              <  1:412162/101‑1 (MQ=255)
                    gACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGc                                                                             <  2:2646275/101‑1 (MQ=255)
                    gACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGc                                                                             >  2:2718106/1‑101 (MQ=255)
                    gACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGc                                                                             <  1:1791138/101‑1 (MQ=255)
                    gACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGc                                                                             >  2:775479/1‑101 (MQ=255)
                      cGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGctc                                                                           <  2:3187260/101‑1 (MQ=255)
                         ttCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCg                                                                        <  2:20104/101‑1 (MQ=255)
                         ttCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCg                                                                        <  2:3594594/101‑1 (MQ=255)
                         ttCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTAGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCg                                                                        >  2:4221274/1‑101 (MQ=255)
                          tCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCgg                                                                       <  1:2149167/101‑1 (MQ=255)
                           cTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCggg                                                                      >  2:3988828/1‑101 (MQ=255)
                            tGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggc                                                                     >  1:103393/1‑101 (MQ=255)
                            tGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggc                                                                     >  1:2484449/1‑101 (MQ=255)
                             gggCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggcg                                                                    >  2:4502846/1‑101 (MQ=255)
                               gCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggcggc                                                                  >  1:378484/1‑101 (MQ=255)
                               gCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggcggc                                                                  >  2:4111802/1‑101 (MQ=255)
                                    aaaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca                                                             >  2:938409/1‑101 (MQ=255)
                                    aaaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca                                                             >  2:4542316/1‑101 (MQ=255)
                                    aaaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca                                                             >  1:2682431/1‑101 (MQ=255)
                                     aaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagcag                                                            >  2:4465555/1‑101 (MQ=255)
                                     aaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagcag                                                            >  2:2281869/1‑101 (MQ=255)
                                     aaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagcag                                                            <  1:3582283/101‑1 (MQ=255)
                                       ccGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAgcagca                                                          <  2:1202776/101‑1 (MQ=255)
                                       ccGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAgcagca                                                          <  1:1610846/101‑1 (MQ=255)
                                       ccGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAgcagca                                                          <  2:4380720/101‑1 (MQ=255)
                                        cGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCaa                                                         <  1:2545587/101‑1 (MQ=255)
                                        cGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCaa                                                         >  2:3919733/1‑101 (MQ=255)
                                          gCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCTGCCAGCAGCAAcg                                                       <  2:704426/101‑1 (MQ=255)
                                          gCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcg                                                       <  2:3263090/101‑1 (MQ=255)
                                           ccGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcgc                                                      >  1:915251/1‑101 (MQ=255)
                                            cGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcgcg                                                     >  2:4168868/1‑101 (MQ=255)
                                              aggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGcgcc                                                   <  1:3810615/99‑1 (MQ=255)
                                              aggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGcgcc                                                   >  1:196902/3‑101 (MQ=255)
                                              aggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGAATCGGCGACAAGGCCGACGTCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGAAACGcgcc                                                   >  2:308714/3‑101 (MQ=255)
                                               ggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGcgccg                                                  >  1:3859326/2‑101 (MQ=255)
                                                gTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGcgccgc                                                 <  1:2925774/101‑1 (MQ=255)
                                                gTCGAACCGCCGGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGCCACGGCCTACGCCTGGCCCACGCCGTTCTGGCGCGGCCAGCAGCAACGgtccgc                                                 >  2:738165/1‑101 (MQ=255)
                                                   gAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCAt                                              >  1:184275/1‑101 (MQ=255)
                                                   gAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCAt                                              >  2:1290626/1‑101 (MQ=255)
                                                   gAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCAt                                              <  2:204438/101‑1 (MQ=255)
                                                   gAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCAt                                              >  1:1423741/1‑101 (MQ=255)
                                                     aCCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCg                                            >  2:2570228/1‑101 (MQ=255)
                                                     aCCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCg                                            <  1:4457436/101‑1 (MQ=255)
                                                       cGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGcc                                          <  1:2818742/101‑1 (MQ=255)
                                                        gCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGccc                                         <  1:1407589/101‑1 (MQ=255)
                                                               cGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGct                                  >  2:3161613/1‑101 (MQ=255)
                                                                 cAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGctct                                <  2:3689613/101‑1 (MQ=255)
                                                                 cAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGctct                                <  2:1778702/101‑1 (MQ=255)
                                                                 cAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGctct                                <  1:3116605/101‑1 (MQ=255)
                                                                 cAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGctct                                >  2:4141486/1‑101 (MQ=255)
                                                                  aGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtg                               >  2:3364720/1‑101 (MQ=255)
                                                                   gCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgc                              >  2:2200223/1‑101 (MQ=255)
                                                                   gCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgc                              <  1:2609951/101‑1 (MQ=255)
                                                                    ccTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgca                             >  2:467813/1‑101 (MQ=255)
                                                                     cTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcat                            <  2:2151033/101‑1 (MQ=255)
                                                                       tCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcatgc                          <  2:837537/101‑1 (MQ=255)
                                                                        cTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcatgca                         <  1:3608881/101‑1 (MQ=255)
                                                                        cTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcatgca                         >  1:4165196/1‑101 (MQ=255)
                                                                          gtccAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGc                       <  1:1734376/99‑1 (MQ=255)
                                                                          ggCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGTCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGc                       <  1:4006920/101‑1 (MQ=255)
                                                                          ggCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCCGc                       >  1:3284326/1‑101 (MQ=255)
                                                                          ggCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGc                       >  1:4594700/1‑101 (MQ=255)
                                                                           gCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGcc                      <  2:1843755/101‑1 (MQ=255)
                                                                             cAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGc                    >  1:895761/1‑101 (MQ=255)
                                                                              aaGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCa                   >  1:279030/1‑101 (MQ=255)
                                                                              aaGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCa                   >  1:3536684/1‑101 (MQ=255)
                                                                              aaGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCa                   <  1:1248187/101‑1 (MQ=255)
                                                                                ggTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAgg                 >  1:4404063/1‑101 (MQ=255)
                                                                                      cATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCttt           <  1:2570380/101‑1 (MQ=255)
                                                                                      cATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCttt           <  2:2894256/101‑1 (MQ=255)
                                                                                       aTCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCtttt          >  1:3251841/1‑101 (MQ=255)
                                                                                         cGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGt        >  1:33691/1‑101 (MQ=255)
                                                                                          ggCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGtt       <  2:2398488/101‑1 (MQ=255)
                                                                                           gCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTc      <  2:2270894/101‑1 (MQ=255)
                                                                                            cGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTcg     <  1:909385/101‑1 (MQ=255)
                                                                                             gACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTcga    >  2:2460221/1‑101 (MQ=255)
                                                                                              gcAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTcgac   <  1:738239/100‑1 (MQ=255)
                                                                                              aCAAGGCCGAGGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTcgac   >  1:4104263/1‑101 (MQ=255)
                                                                                              aCAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTcgac   <  2:378440/101‑1 (MQ=255)
                                                                                               cAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCGAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTcgacg  <  1:195261/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
AATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTCGACG  >  NC_014034/2960488‑2960683

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG