Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,962,711 A→G 100% K21E (AAG→GAG)  hisM → polar amino acid ABC transporter permease HisM

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,962,7110A→G100.0% 837.2 221K21E (AAG→GAG) hisMpolar amino acid ABC transporter permease HisM
Reads supporting (aligned to +/- strand):  new base (99/122):  ref base (0/0):  total (99/122)

Only 100 of 238 total aligned reads displayed.

GCGACGATGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCAAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGGCGCT  >  NC_014034/2962613‑2962808
                                                                                                  |                                                                                                 
 cttccgctctgggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAgc                                                                                                <  2:1129670/91‑1 (MQ=255)
 cgacgaTGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAgc                                                                                                >  2:3131434/1‑101 (MQ=255)
    cgaTGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGGTGCACGCAGATCATCGCGGATTTCCCCTTCCGCTCGCTGGGCTATGGCAGAcgcg                                                                                             >  2:1957611/1‑101 (MQ=255)
    cgaTGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCCCGCTGGGCTATGGCGAGcgcg                                                                                             >  2:2299397/1‑101 (MQ=255)
      aTGGCGGGCGAAGCGCCACGGAAGGCCCCGGCATGAGCGGCAGGCGCGCCCAGAACATCGCGGATTACGCCCTCCGCTCGCTGGGCGTTGGCCAGcgcgag                                                                                           >  2:4066811/1‑99 (MQ=255)
      aTGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGtg                                                                                           <  1:408929/101‑1 (MQ=255)
         gcgggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGGGAGCGCGtagat                                                                                        >  1:846762/1‑97 (MQ=255)
           gggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGcc                                                                                      <  1:3523482/101‑1 (MQ=255)
           gggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGcc                                                                                      <  1:4493755/101‑1 (MQ=255)
           gggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGcc                                                                                      >  1:1215245/1‑101 (MQ=255)
           gggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGcc                                                                                      >  1:2349158/1‑101 (MQ=255)
            ggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCcg                                                                                     >  2:2029812/1‑101 (MQ=255)
              cgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCcgcg                                                                                   <  2:1274645/101‑1 (MQ=255)
               gAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCgcgc                                                                                  >  1:1707053/1‑101 (MQ=255)
                aaGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCa                                                                                 >  2:2169117/1‑101 (MQ=255)
                    gcAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGGGCGCGGGCTGCCGCGCAGTGa                                                                             >  1:1017837/1‑101 (MQ=255)
                    gcAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGa                                                                             >  2:493193/1‑101 (MQ=255)
                        cGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTc                                                                         >  2:3558339/1‑101 (MQ=255)
                         ggAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCa                                                                        <  2:3291313/101‑1 (MQ=255)
                         ggAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCa                                                                        <  2:3761744/101‑1 (MQ=255)
                         ggAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCa                                                                        <  1:4031540/101‑1 (MQ=255)
                         ggAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCa                                                                        >  1:3715681/1‑101 (MQ=255)
                          gAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCAc                                                                       <  2:3263179/101‑1 (MQ=255)
                          gAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCAc                                                                       >  1:3563648/1‑101 (MQ=255)
                            aGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGc                                                                     >  1:2567908/1‑101 (MQ=255)
                             ggCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGct                                                                    <  2:390650/101‑1 (MQ=255)
                              gCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGctc                                                                   <  2:832572/101‑1 (MQ=255)
                                cgcgGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTg                                                                 >  2:4724082/1‑101 (MQ=255)
                                  cgGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGcc                                                               >  1:2238929/1‑101 (MQ=255)
                                  cgGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGcc                                                               >  2:563034/1‑101 (MQ=255)
                                    gCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCAt                                                             >  1:744006/1‑101 (MQ=255)
                                     cATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATg                                                            <  1:3889866/101‑1 (MQ=255)
                                      aTGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGa                                                           >  1:4705210/1‑101 (MQ=255)
                                        gAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAAt                                                         <  1:3825646/101‑1 (MQ=255)
                                          gCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTc                                                       <  1:2169241/101‑1 (MQ=255)
                                           cGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCa                                                      <  1:1776078/101‑1 (MQ=255)
                                           cGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCa                                                      <  2:60597/101‑1 (MQ=255)
                                           cGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCa                                                      <  2:1215155/101‑1 (MQ=255)
                                            gACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCAc                                                     <  2:3351116/101‑1 (MQ=255)
                                            gACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCAc                                                     >  1:3033271/1‑101 (MQ=255)
                                            gACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCAc                                                     <  2:4555120/101‑1 (MQ=255)
                                               aGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCt                                                  <  1:3432602/101‑1 (MQ=255)
                                                gCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTg                                                 >  1:1326519/1‑101 (MQ=255)
                                                   gCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCTCCCTGATc                                              <  2:1772273/101‑1 (MQ=255)
                                                   gCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATc                                              <  2:120960/101‑1 (MQ=255)
                                                     aCGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCgg                                            <  1:3122343/101‑1 (MQ=255)
                                                     aCGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCgg                                            >  2:1446486/1‑101 (MQ=255)
                                                       gCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCt                                          >  1:1620067/1‑101 (MQ=255)
                                                        cAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTc                                         >  2:2426530/1‑101 (MQ=255)
                                                         aGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATTGGCTCg                                        >  1:795786/1‑101 (MQ=255)
                                                         aGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCg                                        <  2:1869420/101‑1 (MQ=255)
                                                          gATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCgg                                       >  2:4642565/1‑101 (MQ=255)
                                                          gATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCgg                                       <  2:2517081/101‑1 (MQ=255)
                                                            tcatcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCgggg                                     >  1:1828250/1‑101 (MQ=255)
                                                             catcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGc                                    <  2:3943850/101‑1 (MQ=255)
                                                             catcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCCGGGc                                    >  2:2956828/1‑101 (MQ=255)
                                                               tcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTTCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTg                                  <  2:3045452/101‑1 (MQ=255)
                                                               tcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTg                                  <  1:1113165/101‑1 (MQ=255)
                                                                cgcgGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGcagatcggaag                                 >  2:4505061/1‑91 (MQ=255)
                                                                 gcgGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGAt                                <  2:68599/101‑1 (MQ=255)
                                                                 gcgGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGAt                                <  1:1447966/101‑1 (MQ=255)
                                                                  cgGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATc                               <  1:2390807/101‑1 (MQ=255)
                                                                   ggATTCCGCCTTCCGCTCGCTGGGCTATGGCGAACGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCt                              <  1:4070267/101‑1 (MQ=255)
                                                                   ggATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCt                              >  2:4696841/1‑101 (MQ=255)
                                                                    gATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTg                             <  2:1039838/101‑1 (MQ=255)
                                                                       tACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTggga                          >  1:4514231/1‑99 (MQ=255)
                                                                        aCGCCTTCCGCTCGCTGGGCTATAGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAAc                         <  2:2597394/101‑1 (MQ=255)
                                                                         cGCTTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTC.GGGCTGATCTGGAACGt                       <  2:2846894/101‑1 (MQ=255)
                                                                         cGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACg                        <  2:1684205/101‑1 (MQ=255)
                                                                          gCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGt                       <  2:331832/101‑1 (MQ=255)
                                                                           ccTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTc                      <  2:2601361/101‑1 (MQ=255)
                                                                            cTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGGACGTCt                     >  2:4466831/1‑101 (MQ=255)
                                                                            cTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCt                     <  2:3677449/101‑1 (MQ=255)
                                                                            cTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTCATCTTGAACGTcc                     >  1:2520159/1‑100 (MQ=255)
                                                                             ttCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTa                    >  1:1980879/1‑101 (MQ=255)
                                                                              tCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAt                   <  1:374383/101‑1 (MQ=255)
                                                                              tCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAt                   <  1:493244/101‑1 (MQ=255)
                                                                              tCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAt                   <  1:1787007/101‑1 (MQ=255)
                                                                               ccGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAtt                  <  1:1108998/101‑1 (MQ=255)
                                                                               ccGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAtt                  <  1:4648977/101‑1 (MQ=255)
                                                                               ccGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAtt                  <  1:1636247/101‑1 (MQ=255)
                                                                                cgctcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAttt                 <  1:1139953/101‑1 (MQ=255)
                                                                                 gctcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTc                <  2:2091514/101‑1 (MQ=255)
                                                                                 gctcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTc                <  1:711051/101‑1 (MQ=255)
                                                                                 gcacgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTc                >  1:2711070/4‑101 (MQ=255)
                                                                                   tcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCgg              <  1:428204/101‑1 (MQ=255)
                                                                                    cgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGGTCTGGAACGCCTATTTCGGc             >  2:373646/1‑101 (MQ=255)
                                                                                    cgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGc             >  2:2217614/1‑101 (MQ=255)
                                                                                     gctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCa            >  2:4364125/1‑101 (MQ=255)
                                                                                     gctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCa            <  2:132295/101‑1 (MQ=255)
                                                                                       tGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACg          <  1:523884/101‑1 (MQ=255)
                                                                                       tGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCGGGTCTGGAACGTCTATTTCGGGACg          >  2:3451706/1‑101 (MQ=255)
                                                                                        gggCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGTTCCGGAACGTCTTTTTCCGCACGc         >  1:3802231/1‑101 (MQ=255)
                                                                                          gCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCGCCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTg       <  2:785189/101‑1 (MQ=255)
                                                                                          gCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGGTCTGGAACGTCTATTTCGGCACGccg       >  1:876020/1‑99 (MQ=255)
                                                                                          gCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTg       >  2:3875843/1‑101 (MQ=255)
                                                                                            tATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGgc     <  2:4112898/101‑1 (MQ=255)
                                                                                            tATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGgc     >  1:1507431/1‑101 (MQ=255)
                                                                                             aTGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGgcg    >  1:416946/1‑101 (MQ=255)
                                                                                               ggCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCCGGGCTGATCTGGAACGTCTATTTCGGCACGCTGGCGCt  >  2:2742091/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GCGACGATGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCAAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGGCGCT  >  NC_014034/2962613‑2962808

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG