Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,973,819 T→A 100% V311D (GTT→GAT)  RCAP_rcc02786 → hemolysin‑type calcium‑binding repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,973,8190T→A100.0% 797.2 208V311D (GTT→GAT) RCAP_rcc02786hemolysin‑type calcium‑binding repeat family protein
Reads supporting (aligned to +/- strand):  new base (102/106):  ref base (0/0):  total (102/106)

Only 100 of 220 total aligned reads displayed.

CCTGCGCAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGTTCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCAATACC  >  NC_014034/2973721‑2973916
                                                                                                  |                                                                                                 
ccTGCGCAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATc                                                                                                 >  1:419647/1‑101 (MQ=255)
 cTGCGCAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCt                                                                                                <  1:1257849/101‑1 (MQ=255)
 cTGCGCAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCt                                                                                                >  1:3140358/1‑101 (MQ=255)
 cTGCGCAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCt                                                                                                >  2:2187419/1‑101 (MQ=255)
    cgcAATGTCGGCGGCGCCATCGACGGGGCGGCGCAGATGGGGGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGGTGTCGAGCTggc                                                                                             >  2:1012421/1‑100 (MQ=255)
    cgcAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTggg                                                                                             <  2:3308172/101‑1 (MQ=255)
     gcAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGgc                                                                                            >  2:2740994/1‑101 (MQ=255)
      cAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGgcg                                                                                           <  1:2455384/101‑1 (MQ=255)
       aaTGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGCTGTCGATCTGGgcgc                                                                                          >  1:1090824/1‑101 (MQ=255)
       aaTGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGgcgc                                                                                          >  2:2257127/1‑101 (MQ=255)
            cggcggCACCATCGACGGGGCGGTGCAGATGGGCGACAGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCggggg                                                                                     >  1:3345756/1‑101 (MQ=255)
             ggcggcACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGc                                                                                    >  1:824941/1‑101 (MQ=255)
             ggcggcACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGc                                                                                    <  1:1678349/101‑1 (MQ=255)
             ggcggcACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGc                                                                                    >  1:1771613/1‑101 (MQ=255)
              gcggcACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGcc                                                                                   >  1:4071829/1‑101 (MQ=255)
               cggcACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCg                                                                                  <  2:2370033/101‑1 (MQ=255)
               cggcACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCg                                                                                  <  1:1395994/101‑1 (MQ=255)
               cggcACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCg                                                                                  >  2:1502760/1‑101 (MQ=255)
                ggcACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGa                                                                                 <  1:4231569/101‑1 (MQ=255)
                ggcACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGa                                                                                 >  1:3717392/1‑101 (MQ=255)
                ggcACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGa                                                                                 >  2:9441/1‑101 (MQ=255)
                 gcACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGAt                                                                                <  2:4554841/101‑1 (MQ=255)
                 gcACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGAt                                                                                <  2:2846855/101‑1 (MQ=255)
                 gcACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGAt                                                                                <  2:1869646/101‑1 (MQ=255)
                  cACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATc                                                                               >  2:4069766/1‑101 (MQ=255)
                   aCCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCg                                                                              >  2:242376/1‑101 (MQ=255)
                    ccATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGc                                                                             >  1:172433/1‑101 (MQ=255)
                      aTCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTa                                                                           >  2:526673/1‑101 (MQ=255)
                      aTCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTa                                                                           <  2:1530369/101‑1 (MQ=255)
                         gACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTacgc                                                                        >  2:1034813/1‑100 (MQ=255)
                          aCGGGGCGGTGCAGATGGGCGGCGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTacgac                                                                       <  2:1090738/101‑1 (MQ=255)
                            ggggCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACgg                                                                     <  1:1884438/101‑1 (MQ=255)
                             gggCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACggg                                                                    >  2:258902/1‑101 (MQ=255)
                             gggCGCTGCAGTTGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCGTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACggg                                                                    <  2:316101/101‑1 (MQ=255)
                                cGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGgcgc                                                                 <  2:1822655/101‑1 (MQ=255)
                                 ggTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCa                                                                >  2:752447/1‑101 (MQ=255)
                                 ggTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCa                                                                <  2:3284262/101‑1 (MQ=255)
                                  gTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAg                                                               <  2:1193314/101‑1 (MQ=255)
                                   tGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAgc                                                              <  1:3850649/101‑1 (MQ=255)
                                   tGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAgc                                                              >  2:1732089/1‑101 (MQ=255)
                                      aGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAgcggc                                                           >  2:3795375/1‑101 (MQ=255)
                                      aGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAgcggc                                                           <  1:211233/101‑1 (MQ=255)
                                      aGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAgcggc                                                           >  1:4706078/1‑101 (MQ=255)
                                      aGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGAGCAgcggg                                                           >  1:2805282/1‑100 (MQ=255)
                                       gATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAgcggcg                                                          >  2:4424952/1‑101 (MQ=255)
                                        aTGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGgcgc                                                         >  1:4520621/1‑101 (MQ=255)
                                        aTGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGgcgc                                                         >  1:3756900/1‑101 (MQ=255)
                                         tGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGgcgcg                                                        >  1:2079272/1‑101 (MQ=255)
                                         tGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGgcgcg                                                        >  1:1910022/1‑101 (MQ=255)
                                            gCGGCGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTg                                                     <  1:2741158/101‑1 (MQ=255)
                                              gACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGa                                                   >  1:3060370/1‑101 (MQ=255)
                                               aCGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGAt                                                  <  1:25070/101‑1 (MQ=255)
                                                cGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATg                                                 >  2:2347542/1‑101 (MQ=255)
                                                cGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATg                                                 >  2:1292689/1‑101 (MQ=255)
                                                 ggggCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATgg                                                >  2:4157604/1‑101 (MQ=255)
                                                 ggggCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATgg                                                >  1:863485/1‑101 (MQ=255)
                                                  gggCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGc                                               <  1:96767/101‑1 (MQ=255)
                                                   ggCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCa                                              <  1:4656653/101‑1 (MQ=255)
                                                   ggCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCa                                              <  1:4601317/101‑1 (MQ=255)
                                                    gCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAg                                             <  2:3575891/101‑1 (MQ=255)
                                                      cGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCg                                           >  2:1939602/1‑101 (MQ=255)
                                                         tCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCg                                        >  2:585234/1‑101 (MQ=255)
                                                            gctgATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATg                                     <  1:4260782/101‑1 (MQ=255)
                                                            gctgATCAATTCGGCCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATg                                     <  1:1008939/101‑1 (MQ=255)
                                                             ctgATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATgg                                    <  2:3606386/101‑1 (MQ=255)
                                                              tgATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATggc                                   >  1:3254269/1‑101 (MQ=255)
                                                              tgATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATggc                                   <  1:358555/101‑1 (MQ=255)
                                                               gATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATggcg                                  >  2:370049/1‑101 (MQ=255)
                                                               gATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATggcg                                  <  1:526724/101‑1 (MQ=255)
                                                               gATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATggcg                                  >  1:4620496/1‑101 (MQ=255)
                                                                aTCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATggcgg                                 <  1:29450/101‑1 (MQ=255)
                                                                  cAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGgcggcg                               >  1:1941645/1‑101 (MQ=255)
                                                                    aTTCGGGCGGGCGGATCCTTGGCGATGTCGATTTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCt                             <  2:1217955/101‑1 (MQ=255)
                                                                     ttCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCtg                            <  2:3291511/101‑1 (MQ=255)
                                                                      tCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGGGCtga                           >  1:2339612/1‑101 (MQ=255)
                                                                      tCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCtga                           <  2:1703583/101‑1 (MQ=255)
                                                                       cgggcgggCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCtgat                          >  1:4398458/1‑101 (MQ=255)
                                                                       cgggcgggCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCtgat                          <  2:3771394/101‑1 (MQ=255)
                                                                        gggcgggcGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCtgatg                         <  2:80114/101‑1 (MQ=255)
                                                                          gcgggcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATgac                       <  1:1571372/101‑1 (MQ=255)
                                                                           cgggcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATgacg                      >  1:1317539/1‑101 (MQ=255)
                                                                           cgggcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATgacg                      >  2:2943491/1‑101 (MQ=255)
                                                                            gggcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATgacga                     >  1:387777/1‑101 (MQ=255)
                                                                            gggcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATgacga                     <  2:3345554/101‑1 (MQ=255)
                                                                            gggcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATgacga                     >  2:4226667/1‑101 (MQ=255)
                                                                              gcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGaca                   <  2:3237839/101‑1 (MQ=255)
                                                                                ggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACAcc                 >  1:259793/1‑101 (MQ=255)
                                                                                ggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACAcc                 <  1:2988936/101‑1 (MQ=255)
                                                                                ggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACAGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACAcc                 >  2:4107239/1‑101 (MQ=255)
                                                                                 gATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCt                >  1:4160585/1‑101 (MQ=255)
                                                                                      ttGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATc           <  1:752521/101‑1 (MQ=255)
                                                                                      ttGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATc           <  2:1112894/101‑1 (MQ=255)
                                                                                      gtGGCGATGTCGGTCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGCCACCTTCATc           <  1:3205031/100‑1 (MQ=255)
                                                                                        ggCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCgg         <  2:656282/101‑1 (MQ=255)
                                                                                         gCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGc        <  1:585285/101‑1 (MQ=255)
                                                                                          cGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCa       >  2:4684678/1‑101 (MQ=255)
                                                                                            aTGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCAAt     >  1:222140/1‑101 (MQ=255)
                                                                                             tGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCAATa    <  1:1641011/101‑1 (MQ=255)
                                                                                              gTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCAATAc   >  2:4720887/1‑101 (MQ=255)
                                                                                               tCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGTGGCGCTGATGACGACACCTTCATCGGCAATAcc  <  2:863411/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CCTGCGCAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGTTCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCAATACC  >  NC_014034/2973721‑2973916

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG