Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,995,606 C→A 9.0% intergenic (+146/+74) RCAP_rcc02802 → / ← RCAP_rcc02803 membrane dipeptidase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,995,6060C→A 9.0% 2.1 ‑2.6 ‑1.240intergenic (+146/+74)RCAP_rcc02802/RCAP_rcc02803membrane dipeptidase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (9/27):  total (12/28)
Fisher's exact test strand distribution p-value = 7.28e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.80e-02

CTTCGCCCCCGGACCCCCAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCG  >  NC_014034/2995506‑2995704
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cTTCGCCCCCGGACCCCCAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.c                                                                                                         <  1:2672119/101‑1 (MQ=255)
cTTCGCCCCCGGACCCCCAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.c                                                                                                         <  1:2461802/101‑1 (MQ=255)
  tCGCCCCCGGACCCCCAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.Caa                                                                                                       <  2:2083625/101‑1 (MQ=255)
   cGCCCCCGGACCCCCAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.Caaa                                                                                                      <  2:659638/101‑1 (MQ=255)
   cGCCCCCGGACCCCCAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.Caaa                                                                                                      <  1:1915601/101‑1 (MQ=255)
   cGCCCCCGGACCCCCAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.Caaa                                                                                                      <  2:1373861/101‑1 (MQ=255)
    gCCCCCGGACCCCCAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.Caaaa                                                                                                     <  1:3876239/101‑1 (MQ=255)
    gCCCCCGGACCCCCAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.Caaaa                                                                                                     <  2:4029970/101‑1 (MQ=255)
    gCCCCCGGACCCCCAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.Caaaa                                                                                                     <  1:2549233/101‑1 (MQ=255)
     cccccGGACCCCCAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAt                                                                                                    <  1:60106/101‑1 (MQ=255)
     cccccGGACCCCCAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAt                                                                                                    <  1:239885/101‑1 (MQ=255)
      ccccGGACCCCCAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.ACGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.a                                                                                                  <  1:3915377/101‑1 (MQ=255)
       cccGGACCCCCAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.Ac                                                                                                 <  2:1519035/101‑1 (MQ=255)
       cccGGACCCCCAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.Ac                                                                                                 <  1:1511932/101‑1 (MQ=255)
        ccGGACCCCCAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACg                                                                                                <  2:2682435/101‑1 (MQ=255)
        ccGGACCCCCAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGCTTG.CCGCCCCGCAACCCGTCGGCTGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACg                                                                                                <  2:3945975/101‑1 (MQ=255)
         cGGACCCCCAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.c                                                                                              <  1:2100218/101‑1 (MQ=255)
                ccAAGAGGCGTATTTTGCCAAGGAAACCCCCTTCGGTTC.CCGCCCCGCACCCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.CCgggggg                                                                                       <  1:4241144/101‑1 (MQ=255)
                 cAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGt                                                                                      >  1:1617129/1‑101 (MQ=255)
                   aggggCTTATTTGGCCAAGGAAACCCCCTTCGGTTG.CCGCCCCGCACCCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGc                                                                                    <  2:1711956/98‑1 (MQ=37)
                   agagGCGTTTTTTGCCAAGAAAACCCCCTTGGGTTC.CCGCCCCGCACCCCGTCGGCGCCCCAGCGCCCTGCTTTTCCTTGG.AAAAAT.ACG.CCGGGGGGTGc                                                                                    <  2:3587280/101‑1 (MQ=25)
                   agagGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGc                                                                                    <  2:4729626/101‑1 (MQ=255)
                    gagGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGCg                                                                                   <  2:2875893/101‑1 (MQ=255)
                     agGCGTATTTTGCCAAGGAAACCCCCTTCGGCTG.CCGCCCCGCAGCCCGTCGGCGGCCCGGCGCCCTGCTTTTCCTTCG.CAAACT.ACG.CCGGGGGGTGCgg                                                                                  <  2:4735559/101‑1 (MQ=37)
                      ggCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGCggg                                                                                 <  2:702095/101‑1 (MQ=255)
                          ttgtttGCCAAGGAAAACCCCCTCGGGTGCCCCCCCCGCACCCGGCGGGCGGCCCAGCCCCTTGTTTTTCCTTGG.AAAAAT.ACG.CCGGGGGGTGCgggggg                                                                              <  2:3437670/98‑1 (MQ=16)
                           aTTTTGCCAAGGAAACCCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGCGGGGGGCg                                                                            <  2:749327/101‑1 (MQ=255)
                           aTTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGCGGGGGGCg                                                                            >  2:19752/1‑101 (MQ=255)
                             tttGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGCGGGGGGCGGa                                                                          <  1:3844525/101‑1 (MQ=255)
                             tttGCCAAGAAACCCCCCTTCGTTTC.CCCCCCCGCACCCCGTCGGCGGCCCAGCCCCTTGTTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGCGGGGGGCGGa                                                                          <  2:3212058/101‑1 (MQ=18)
                              ttGCCAAGGAAACCCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGCGGGGGGCGGAg                                                                         <  2:4203650/101‑1 (MQ=255)
                               tGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGCGGGGGGCGGAGc                                                                        >  2:362088/1‑101 (MQ=255)
                                gCCAAGGAAACCCCCTTCGGTTG.CCGCCCCGCAGCCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGCGGGGGGCGGAGcc                                                                       <  1:362122/101‑1 (MQ=255)
                                gCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGCGGGGGGCGGAGcc                                                                       <  2:3173967/101‑1 (MQ=255)
                                 caaagaaaccccccTTCGGTCC.CCCCCCCGCCCCCCGGGGGC.CCCCCCCCCCCTTTTTTTTCTTGGAAAAAAG.CCC.CCGGGGGGTGCGGGGGGCGGAGccc                                                                      <  1:1623871/91‑1 (MQ=2)
                                   aaGGAAACCCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGCGGGGGGCGGAGccccc                                                                    <  2:1944041/101‑1 (MQ=255)
                                     ggAACACCCCTTCGGTTC.CCGCCCCGCACCCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCcg                                                                  <  1:4384569/101‑1 (MQ=255)
                                       gaaaCCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CACAAT.ACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCcc.cg                                                               >  1:2061467/2‑101 (MQ=255)
                                           ccccTTCGTTTC.CCGCCCCGCACCCCGTGGGCGGCCCGGCGCCCTGTTTTTCTTGGG.AAAATT.ACG.CGGGGGGGTGCGGGGGGGGGGGCCCCCCG.CGGGTc                                                           <  2:1544220/101‑1 (MQ=16)
                                            cccTGCGGGTG.CGGCCCCGAAGCCCGTGGGGGGCCCCGCCCCTTGCTTTGCCTGGC.CAAAAG.GGG.GGGGGGGGGGGGGGGGGCGGAGCCCCCCG.CGGGTCg                                                          <  2:1929713/101‑1 (MQ=9)
                                               ttCGGTTG.CCCCCGCGCACCCCGTCGGCGGCCCAGCGCCCTGCTTTCCCTTGG.CAAAAT.AGG.CCGGGGGGTCCGGGGGGCGGAGCCCCCCG.CGGGTCGccc                                                       <  2:3893328/101‑1 (MQ=18)
                                                       ccccccccCCACCCCGTGGGCGCCCCAGCCCCCTTTTTTCCCGGGG.CAAAAT.CCG.CGGGGGGGTGGGGGGGGGGGAGCCCCCCG.CGGGTCGCCCCGGCTT..g                                              <  1:1419898/97‑1 (MQ=12)
                                                                    ccGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGGCGCCCCGGCTT..GCCGGGGCGGAATc                                 >  2:4057193/1‑101 (MQ=255)
                                                                    ccGCCGGCGCCCCAGCCCCCTGCTTTTCCTGGG.AAAAAT.GGG.CGGGGGGGTGGGGGGGGGGGACCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATc                                 <  1:4601521/101‑1 (MQ=18)
                                                                       gccgcggcCCCGGCCCCTTTTTTTCCTTGG.GAAAATCGCG.CGGGGGGGGGGGGGGGGGGGCCCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATccc                               <  2:2570271/98‑1 (MQ=16)
                                                                            ggcCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAAGCCCCCGCTc                         >  1:686689/1‑101 (MQ=255)
                                                                                   cccccTGCTTTTCCTTGG.CAAAAT.CCG.CGGGGGGGTGGGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCa                  <  2:2924081/99‑1 (MQ=255)
                                                                                       cTGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAgaga              >  1:632652/1‑101 (MQ=255)
                                                                                        tGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGTCTCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCagaggc             >  2:3927275/1‑99 (MQ=255)
                                                                                          tttttCCTTGG.AAAAAT.ACGCCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCCGGGTCGCCTCGGCGTCAGCCGAGGCGAAATACCCC.CTCAGCccttcatc              >  2:2611488/2‑95 (MQ=0)
                                                                                             ttCCTTGG.AAAAAT.ACGCCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCCGGGTCGCCTCGGCGTCCGCCGAGGCGAAATACCCC.CTCAGCccttcatcttg           >  1:789505/1‑92 (MQ=0)
                                                                                             ttCCTTGG.AAAAAT.ACGCCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCCGGGTCGCCTCGGCGTCAGCCGAGGCGAAATACCCC.CTCAGCccttcatcttg           >  2:4078231/1‑92 (MQ=0)
                                                                                                   gg.CAAAAT.ACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGAccccc  >  1:1263423/1‑98 (MQ=255)
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CTTCGCCCCCGGACCCCCAAGAGGCGTATTTTGCCAAGGAAAACCCCTTCGGTTG.CCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAAT.ACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCG  >  NC_014034/2995506‑2995704

Base quality scores:  ATCG  < 3 ≤  ATCG  < 14 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG