Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,020 A→C 9.8% intergenic (+66/+110) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0200A→C 9.8% 50.0 ‑0.7 ‑0.4236intergenic (+66/+110)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (12/11):  ref base (91/117):  total (106/130)
Fisher's exact test strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.94e-01

Only 100 of 259 total aligned reads displayed.

GATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGG  >  NC_014034/3003921‑3004119
                                                                                                   |                                                                                                    
 ttgatCGCTCTGGTGCGCGCGTGCGAGATCTGAGGGCGAGGCGTGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGAcc                                                                                                    <  2:1134231/100‑1 (MQ=37)
 atgatCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGAcc                                                                                                    <  1:369009/101‑1 (MQ=255)
   gatcgatcTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCaa                                                                                                  <  1:1365222/101‑1 (MQ=255)
    atcgatcTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAg                                                                                                 <  1:4191028/101‑1 (MQ=255)
    atcgatcTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAg                                                                                                 <  2:967232/101‑1 (MQ=255)
      cgatcTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAggg                                                                                               >  2:1012592/1‑100 (MQ=255)
       gatcTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTg                                                                                              <  1:1161769/101‑1 (MQ=255)
       gatcTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTg                                                                                              <  2:657666/101‑1 (MQ=255)
       gatcTACTGCGCGCGGGCGAGATCTGAGGGCGAGGCGGGGGGCGCTGCCCCACTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTg                                                                                              <  1:4652732/101‑1 (MQ=37)
        atcTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGa                                                                                             <  1:30344/101‑1 (MQ=255)
          cTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGaaa                                                                                           <  1:213542/101‑1 (MQ=255)
          cTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGaaa                                                                                           <  2:2758834/101‑1 (MQ=255)
          cTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGaaa                                                                                           <  2:600382/101‑1 (MQ=255)
           tGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAg                                                                                          <  2:794202/101‑1 (MQ=255)
           tGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAg                                                                                          <  2:3667476/101‑1 (MQ=255)
               gcgcgcGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.a                                                                                     <  2:2779083/101‑1 (MQ=255)
                cgcgcgGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.At                                                                                    <  1:2626912/101‑1 (MQ=255)
                  cgcgGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATcc                                                                                  <  2:1191729/101‑1 (MQ=255)
                   gcgGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATccc                                                                                 <  2:1402680/101‑1 (MQ=255)
                     gggCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCgg                                                                               <  1:1039795/101‑1 (MQ=255)
                      ggCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTAACCCCCCGGGATATTTGGACCAAGGAGAAAGCAG.ATCCCGGt                                                                              >  1:3789200/1‑101 (MQ=255)
                       gCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGtt                                                                             >  2:247885/1‑101 (MQ=255)
                        ggagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGttt                                                                            <  1:3420506/100‑1 (MQ=255)
                        cGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCGATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGttt                                                                            <  2:2604227/101‑1 (MQ=255)
                        cGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGttt                                                                            >  1:2852793/1‑101 (MQ=255)
                         gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACTCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTg                                                                           >  2:312810/1‑101 (MQ=255)
                         aagaGCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTg                                                                           <  1:4476455/100‑1 (MQ=255)
                           gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                         >  1:3199351/1‑101 (MQ=255)
                           gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGGGAAAGCAG.ATCCCGGTTTGcc                                                                         >  1:68448/1‑101 (MQ=255)
                             tCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCtt                                                                       <  1:1607497/101‑1 (MQ=255)
                               tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                     <  2:2091994/101‑1 (MQ=255)
                               tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                     <  1:1719218/101‑1 (MQ=255)
                                gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                    <  2:2961727/101‑1 (MQ=255)
                                gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                    <  2:1100032/101‑1 (MQ=255)
                                gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                    <  1:19770/101‑1 (MQ=255)
                                  gggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCAcc                                                                  >  2:2895738/1‑101 (MQ=255)
                                   ggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCt                                                                 <  1:3986657/101‑1 (MQ=255)
                                   ggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCt                                                                 <  1:2959037/101‑1 (MQ=255)
                                   ggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCt                                                                 <  2:3681507/101‑1 (MQ=255)
                                    gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCAGCtt                                                                <  1:4235841/101‑1 (MQ=255)
                                    gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCtt                                                                <  2:4733040/101‑1 (MQ=255)
                                    gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCtt                                                                >  1:4458149/1‑101 (MQ=255)
                                     cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTg                                                               >  1:522414/1‑101 (MQ=255)
                                      aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTgg                                                              >  2:4610590/1‑101 (MQ=255)
                                      aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCCCACGGCCAATTCACCCCCCCGCAAATTTTGGCCAAGGGGGGAGCAG.ACCCCGGTTTGCCTTTCCCCTTgg                                                              >  1:2415385/1‑101 (MQ=21)
                                        ggCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGgcg                                                            >  2:282998/1‑101 (MQ=255)
                                          cGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCa                                                          >  1:643338/1‑101 (MQ=255)
                                           ggggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCCCCTTGGCGCaa                                                         >  2:3619322/1‑101 (MQ=255)
                                             ggggggtccccccccTCCTGTCCTGACGGCCAATTCCCCCCCCGGGATATTTCGCCCAAGGCAAAACCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                       <  2:4314694/92‑1 (MQ=25)
                                             ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                       <  2:1516039/101‑1 (MQ=255)
                                             gaggCGCTGCCCCCCTCTGGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGCCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                       <  1:1144067/99‑1 (MQ=255)
                                               ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                     <  1:219207/101‑1 (MQ=255)
                                                gcgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATc                                                    <  2:2061610/101‑1 (MQ=255)
                                                 cgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATcc                                                   >  1:2210682/1‑101 (MQ=255)
                                                    tGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgg                                                <  1:3242623/101‑1 (MQ=255)
                                                      ccccccTCTTGGCCT.TCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGAT.AAAGAAG.GTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagccc                                        >  2:3977735/1‑96 (MQ=37)
                                                        ccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgggggg                                            >  2:2411894/1‑101 (MQ=255)
                                                        ccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgggggg                                            >  2:2360/1‑101 (MQ=255)
                                                           ctctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGCCGCAAATATCCCGGGGGGAGt                                         <  1:3494056/101‑1 (MQ=255)
                                                            tctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTc                                        >  1:563196/1‑101 (MQ=255)
                                                              ttGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTccc                                      >  2:751411/1‑101 (MQ=255)
                                                               tGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                     >  1:2874867/1‑101 (MQ=255)
                                                               tGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                     >  2:1610889/1‑101 (MQ=255)
                                                                ggCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                    >  1:406093/1‑101 (MQ=255)
                                                                  ccTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgg                                  >  2:1877623/1‑101 (MQ=255)
                                                                      aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                              <  1:2724240/101‑1 (MQ=255)
                                                                       cGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACg                             <  1:312844/101‑1 (MQ=255)
                                                                        ggCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgg                            >  1:1468305/1‑101 (MQ=255)
                                                                           cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                         >  2:4674720/1‑101 (MQ=255)
                                                                           cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                         <  2:2679205/101‑1 (MQ=255)
                                                                            gcttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                        <  2:2144979/99‑1 (MQ=255)
                                                                             aTTCGCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.GTCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                       <  2:4409774/101‑1 (MQ=255)
                                                                             aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                       >  2:3373161/1‑101 (MQ=255)
                                                                             aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                       <  2:2729936/101‑1 (MQ=255)
                                                                             aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCCGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGGGGCCCGCGGGGGCggggggg                       >  2:4024223/1‑100 (MQ=21)
                                                                              ttCCCCCCCCGGGATATTTGGACAAGGGTGAAAGCAG.ATCCCGGTTTGCTTTTCCCCTTGGCGAAAATACCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                      <  1:1012671/101‑1 (MQ=21)
                                                                               tccccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTGTGCCTTTCCCGTTGGCGGAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                     <  1:1266961/98‑1 (MQ=18)
                                                                               tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                     <  1:368730/101‑1 (MQ=255)
                                                                               cccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGGGTCCCGCAGGGACGGGGGGCag                     >  2:3745347/4‑101 (MQ=18)
                                                                                ccccccccGGGATATTTGGCCCAAGGTGAAATCCC.CCCCCCGTGTTCATTTCCCCTTGGCGGAAATATCCCGGTGGGAGTCCCGCAGGGACGGGGGGCagc                    <  1:460664/99‑2 (MQ=17)
                                                                                ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                    <  2:1966374/99‑2 (MQ=18)
                                                                                 cccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                   <  1:267058/100‑1 (MQ=255)
                                                                                 cccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                   <  2:3933354/100‑1 (MQ=255)
                                                                                 aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                   <  1:3180309/101‑1 (MQ=255)
                                                                                  ccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                  <  1:2484539/101‑1 (MQ=18)
                                                                                   cccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                 >  1:2758194/1‑101 (MQ=18)
                                                                                     cccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccc               <  1:1638962/101‑1 (MQ=255)
                                                                                       cGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc             >  2:4382420/1‑101 (MQ=18)
                                                                                        gggATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccc            <  1:4502560/101‑2 (MQ=17)
                                                                                        gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct            >  2:4440822/1‑101 (MQ=255)
                                                                                           atatTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct         >  2:2282405/1‑97 (MQ=18)
                                                                                           atatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc         >  1:474038/1‑101 (MQ=255)
                                                                                           atatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc         >  2:3501376/1‑101 (MQ=255)
                                                                                            tttttGGCCAAAGGTGAAAGCAG.ATCCCGTTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc        <  2:3271633/99‑1 (MQ=255)
                                                                                            tatTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt        >  1:2452594/1‑96 (MQ=18)
                                                                                            tatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt        <  2:1680358/101‑6 (MQ=17)
                                                                                               ttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc     >  1:4351023/1‑101 (MQ=255)
                                                                                               ttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc     <  1:75970/101‑1 (MQ=255)
                                                                                                 ggACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg   >  1:385777/1‑101 (MQ=255)
                                                                                                 ggACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg   >  2:3033763/1‑101 (MQ=255)
                                                                                                   |                                                                                                    
GATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGG  >  NC_014034/3003921‑3004119

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG