Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,037 T→G 8.1% intergenic (+83/+93) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0370T→G 8.1% 42.6 ‑0.2 ‑0.2230intergenic (+83/+93)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (9/10):  ref base (106/102):  total (118/112)
Fisher's exact test strand distribution p-value = 8.14e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.60e-01

Only 100 of 252 total aligned reads displayed.

CGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGT  >  NC_014034/3003937‑3004137
                                                                                                       |                                                                                                    
  cgcgGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATcc                                                                                                    <  2:1191729/101‑1 (MQ=255)
       gCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGtt                                                                                               >  2:247885/1‑101 (MQ=255)
        ggagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGttt                                                                                              <  1:3749906/100‑1 (MQ=255)
         gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTg                                                                                             <  1:1260126/101‑1 (MQ=255)
           gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCCCCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                                           <  1:2736103/101‑1 (MQ=255)
           gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                                           <  1:1465239/101‑1 (MQ=255)
           gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGGGAAAGCAG.ATCCCGGTTTGcc                                                                                           >  1:68448/1‑101 (MQ=255)
            aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCt                                                                                          <  2:3646444/101‑1 (MQ=255)
             tCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCtt                                                                                         <  2:4404698/101‑1 (MQ=255)
               tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                                       <  2:3065736/101‑1 (MQ=255)
               tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                                       <  2:441928/101‑1 (MQ=255)
               tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                                       <  1:1719218/101‑1 (MQ=255)
                gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                                      <  2:1100032/101‑1 (MQ=255)
                gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                                      <  1:19770/101‑1 (MQ=255)
                gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGAAG.ATCCCGGTTTGCCTTTCa                                                                                      >  2:3567171/1‑101 (MQ=255)
                 aGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCCGACCGGCCAATTACCCCCCC.CCGGCATTTTGTCCAAGGCGAACGCAA.ACTCCCGCTTGCCTTTcc                                                                                      >  1:3255272/1‑100 (MQ=255)
                  gggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCAcc                                                                                    >  2:2937242/1‑101 (MQ=255)
                   ggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCt                                                                                   <  1:3986657/101‑1 (MQ=255)
                    gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCAGCtt                                                                                  <  1:4235841/101‑1 (MQ=255)
                    gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCtt                                                                                  >  1:4458149/1‑101 (MQ=255)
                    gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCtt                                                                                  <  2:4733040/101‑1 (MQ=255)
                     cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTg                                                                                 <  1:342625/101‑1 (MQ=255)
                      aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTgg                                                                                >  2:4610590/1‑101 (MQ=255)
                      aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GCGGTATTTGGCCCCAGGGGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTgg                                                                                >  1:2761781/1‑101 (MQ=37)
                      aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCCCA.CGGCCAATTCACCCCCC.CGCAAATTTTGGCCAAGGGGGGAGCAG.ACCCCGGTTTGCCTTTCCCCTTgg                                                                                >  1:2415385/1‑101 (MQ=21)
                       aGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGgc                                                                               <  1:3813485/101‑1 (MQ=255)
                        tgCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGgcg                                                                              <  1:4295842/100‑1 (MQ=255)
                         gCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGgcgc                                                                             >  2:2780868/1‑101 (MQ=255)
                             ggggggtccccccccTCCTGTCCTGA.CGGCCAATTCCCCCCCC.GGGATATTTCGCCCAAGGCAAAACCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                         <  2:4314694/92‑1 (MQ=25)
                             ggggCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCCCCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                         <  1:4684495/101‑1 (MQ=255)
                             ggggCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                         <  2:1516039/101‑1 (MQ=255)
                             ggggCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                         >  1:2679359/1‑101 (MQ=255)
                               ggCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                       <  1:219207/101‑1 (MQ=255)
                               ggCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                       <  1:2606611/101‑1 (MQ=255)
                                 cgcTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATcc                                                                     >  1:2210682/1‑101 (MQ=255)
                                   cTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCg                                                                   <  1:573122/101‑1 (MQ=255)
                                    tGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgg                                                                  >  2:2078309/1‑101 (MQ=255)
                                     gCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                 >  2:1128896/1‑101 (MQ=255)
                                      ccccccTCTTGGCCT.T.CGGCCAATTCACCCCCC.GGGATATTTCGGGCAAGAT.AAAGAAG.GTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagccc                                                          >  2:1846795/1‑96 (MQ=37)
                                       cccccTCTTGGCCTGA.CGGCCAATTCACCCCCCGGGGAATTTTGGCCCAGGGGGAAAAAAA.ACCCCGGTTGCCCTTTTCCCTTGCGCCAAAAATCCGgggg                                                                >  1:3214124/1‑101 (MQ=9)
                                       cccccTCTTGGCCT.T.CGGCCAATTCCCCCCCC.GGGATATTTCGGGCAAGAT.AAAGAAG.GTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagcccg                                                         <  1:287408/101‑7 (MQ=37)
                                        ccccTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgggggg                                                              >  2:2411894/1‑101 (MQ=255)
                                          ccTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAg                                                            <  1:4469488/101‑1 (MQ=255)
                                           ctctTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGCTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGt                                                           >  2:1438952/1‑101 (MQ=255)
                                            tctTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTc                                                          >  2:2621312/1‑101 (MQ=255)
                                            tctTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTc                                                          <  2:4516520/101‑1 (MQ=255)
                                             ctTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTcc                                                         >  1:3194945/1‑101 (MQ=255)
                                              ttGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTccc                                                        >  2:751411/1‑101 (MQ=255)
                                               tGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                       <  2:4348233/101‑1 (MQ=255)
                                                ggCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                      >  2:198119/1‑101 (MQ=255)
                                                ggCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                      >  2:920737/1‑101 (MQ=255)
                                                ggCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                      >  1:406093/1‑101 (MQ=255)
                                                    tGA.CGGCCAATTCGCCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                  <  1:3172244/101‑1 (MQ=255)
                                                     gA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGa                                                 >  2:1197034/1‑101 (MQ=255)
                                                      a.CGGCCAATTCCCCCCCC.GGGATATTTGGCCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                                                <  2:673217/101‑1 (MQ=255)
                                                        cGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACg                                               <  1:312844/101‑1 (MQ=255)
                                                            cAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                                           >  2:4674720/1‑101 (MQ=255)
                                                             gcttCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                          <  2:2144979/99‑1 (MQ=255)
                                                             aaTTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                          >  1:2087249/1‑101 (MQ=255)
                                                              aTTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                         <  2:2729936/101‑1 (MQ=255)
                                                               ttCCCCCCCC.GGGATATTTGGACAAGGGTGAAAGCAG.ATCCCGGTTTGCTTTTCCCCTTGGCGAAAATACCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                        <  1:1012671/101‑1 (MQ=21)
                                                                tCACCCCCC.GGGATATTTGGACCAAGGTGAAGGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                       >  2:2097362/1‑101 (MQ=255)
                                                                tCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                       <  1:368730/101‑1 (MQ=255)
                                                                 cccccccc.GGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                      <  2:2893325/99‑2 (MQ=18)
                                                                 cACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                      >  1:1480378/1‑101 (MQ=255)
                                                                 cACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                      >  1:2635727/1‑101 (MQ=255)
                                                                  ccccccc.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                     <  2:3933354/100‑1 (MQ=255)
                                                                   cccccc.GGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                    <  1:2484539/101‑1 (MQ=18)
                                                                    ccccc.GGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                   >  1:102579/1‑101 (MQ=18)
                                                                     cccc.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAggcc                                  >  2:1614622/1‑101 (MQ=255)
                                                                      ccc.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccc                                 <  1:1638962/101‑1 (MQ=255)
                                                                       cc.GGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccc                                >  2:2337334/1‑101 (MQ=17)
                                                                        c.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccc                               <  2:3175555/101‑1 (MQ=255)
                                                                          gggATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccc                              <  1:4502560/101‑2 (MQ=17)
                                                                          gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCTCGGGGGGAGTCCCGCGGGGGCGGGGGGCAGAgcccccct                              <  1:502862/101‑1 (MQ=255)
                                                                           ggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctg                             <  2:3688645/101‑1 (MQ=255)
                                                                            gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGAGCAGAgcccacctgc                            >  1:4614400/1‑101 (MQ=255)
                                                                             atatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc                           >  2:3501376/1‑101 (MQ=255)
                                                                              tttttGGCCAAAGGTGAAAGCAG.ATCCCGTTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                          <  2:3271633/99‑1 (MQ=255)
                                                                              tatTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                          >  1:2452594/1‑96 (MQ=18)
                                                                              tatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                          <  2:3997574/101‑6 (MQ=17)
                                                                              tatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                          <  2:1680358/101‑6 (MQ=17)
                                                                               atTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                         >  1:2973536/1‑95 (MQ=18)
                                                                               atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCCGGGGCAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                         >  2:1886475/1‑101 (MQ=255)
                                                                                 ttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                       <  1:75970/101‑1 (MQ=255)
                                                                                 ttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                       >  1:2885002/1‑101 (MQ=255)
                                                                                 ttGGACCAAGGTGAAAGCAG.AGCCCGGTGTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                       <  1:1151881/101‑1 (MQ=255)
                                                                                   ggACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                     >  2:3033763/1‑101 (MQ=255)
                                                                                   ggACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                     >  1:4602673/1‑101 (MQ=255)
                                                                                    gACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgg                    >  1:548285/1‑101 (MQ=255)
                                                                                      ccAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgggg                  <  1:4126745/101‑1 (MQ=255)
                                                                                       cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCCGCCCCCCTGGGGc                 >  1:1121768/1‑101 (MQ=255)
                                                                                        aaGGTGAAAGCAG.ATCCCGTTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                <  2:252846/101‑1 (MQ=255)
                                                                                         aGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg               <  2:3788965/101‑1 (MQ=255)
                                                                                         aGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg               >  1:1311826/1‑101 (MQ=255)
                                                                                          ggTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa              <  2:2761617/101‑1 (MQ=255)
                                                                                                   aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg     >  2:4122377/1‑101 (MQ=255)
                                                                                                   aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg     >  2:2359562/1‑101 (MQ=255)
                                                                                                    g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc    >  1:387800/1‑101 (MQ=255)
                                                                                                      aTCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCg   >  1:3533339/1‑101 (MQ=255)
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CGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCACCCCCC.GGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGT  >  NC_014034/3003937‑3004137

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG