Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,041 G→C 8.0% intergenic (+87/+89) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0410G→C 8.0% 37.0 ‑0.2 ‑0.1229intergenic (+87/+89)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (9/9):  ref base (110/97):  total (121/108)
Fisher's exact test strand distribution p-value = 8.11e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.57e-01

Only 100 of 258 total aligned reads displayed.

CGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTT  >  NC_014034/3003941‑3004141
                                                                                                      |                                                                                                    
    ggagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGttt                                                                                                  <  1:3420506/100‑1 (MQ=255)
    cGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCGATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGttt                                                                                                  <  2:2604227/101‑1 (MQ=255)
    cGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGttt                                                                                                  >  1:2852793/1‑101 (MQ=255)
     gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTg                                                                                                 <  1:1260126/101‑1 (MQ=255)
     gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTg                                                                                                 <  1:2415615/101‑1 (MQ=255)
       gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                                               <  1:1465239/101‑1 (MQ=255)
       gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                                               >  1:3199351/1‑101 (MQ=255)
        aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCt                                                                                              <  1:2987196/101‑1 (MQ=255)
         tCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACTGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCtt                                                                                             <  2:3943887/101‑1 (MQ=255)
         tCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCtt                                                                                             <  1:1607497/101‑1 (MQ=255)
          cTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCttt                                                                                            >  1:685404/1‑101 (MQ=255)
           tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                                           <  2:3065736/101‑1 (MQ=255)
           tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                                           >  1:4414958/1‑101 (MQ=255)
            gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                                          <  2:1100032/101‑1 (MQ=255)
            gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                                          <  1:19770/101‑1 (MQ=255)
              gggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCAcc                                                                                        >  2:2937242/1‑101 (MQ=255)
               ggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCt                                                                                       <  1:3986657/101‑1 (MQ=255)
               ggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCt                                                                                       <  2:3681507/101‑1 (MQ=255)
               ggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCt                                                                                       <  1:2959037/101‑1 (MQ=255)
                gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCtt                                                                                      <  2:4733040/101‑1 (MQ=255)
                 cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTg                                                                                     >  1:522414/1‑101 (MQ=255)
                 cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTg                                                                                     <  1:2273407/101‑1 (MQ=255)
                   aGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGgc                                                                                   <  1:3813485/101‑1 (MQ=255)
                    ggCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGgcg                                                                                  >  2:282998/1‑101 (MQ=255)
                     gCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGgcgc                                                                                 >  2:2780868/1‑101 (MQ=255)
                     gCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGgcgc                                                                                 <  1:2143489/101‑1 (MQ=255)
                      cGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCa                                                                                >  1:643338/1‑101 (MQ=255)
                       ggggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCCCCTTGGCGCaa                                                                               >  2:3619322/1‑101 (MQ=255)
                        gggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCaaa                                                                              <  1:4511483/101‑1 (MQ=255)
                         ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                             <  2:1516039/101‑1 (MQ=255)
                         ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                             <  1:4463576/101‑1 (MQ=255)
                         ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                             <  1:3623501/101‑1 (MQ=255)
                          gggCGCTGCCCCCCTCTTGGCCCGACGGCCAATTCACCCCCCCGGGAAATTTGGCCCCAGGGAAAAATAA.AACCGGGTTGTCCTTTTCCCTTTGCCCaaaa                                                                             >  1:4038576/1‑100 (MQ=12)
                           ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                           <  1:2606611/101‑1 (MQ=255)
                           ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                           <  1:219207/101‑1 (MQ=255)
                           ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTC..CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATc                                                                          <  2:2495297/101‑1 (MQ=255)
                                tGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgg                                                                      <  1:3242623/101‑1 (MQ=255)
                                tGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgg                                                                      >  2:2078309/1‑101 (MQ=255)
                                 gCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                     >  2:1128896/1‑101 (MQ=255)
                                 gCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                     >  1:4295272/1‑101 (MQ=255)
                                  ccccccTCTTGGCCT.TCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGAT.AAAGAAG.GTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagccc                                                              >  2:1846795/1‑96 (MQ=37)
                                    ccccTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgggggg                                                                  >  2:2360/1‑101 (MQ=255)
                                    ccccTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgggggg                                                                  >  2:2411894/1‑101 (MQ=255)
                                       ctctTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGCCGCAAATATCCCGGGGGGAGt                                                               <  1:3494056/101‑1 (MQ=255)
                                           tGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                           >  1:2874867/1‑101 (MQ=255)
                                            ggCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                          >  2:198119/1‑101 (MQ=255)
                                            ggCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                          >  2:920737/1‑101 (MQ=255)
                                                tGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                      <  2:4207429/101‑1 (MQ=255)
                                                tGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                      >  1:4211298/1‑101 (MQ=255)
                                                  aCGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                                                    <  1:2724240/101‑1 (MQ=255)
                                                      ccAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggg                                                >  2:4507949/1‑101 (MQ=255)
                                                         aTTCG.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.GTCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                             <  2:4409774/101‑1 (MQ=255)
                                                         aTTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                             <  2:4392376/101‑1 (MQ=255)
                                                         aTTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                             >  2:3373161/1‑101 (MQ=255)
                                                         aTTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                             <  2:2729936/101‑1 (MQ=255)
                                                         aTTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCCGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGGGGCCCGCGGGGGCggggggg                                             >  2:4024223/1‑100 (MQ=21)
                                                          ttCC.CCCCCCGGGATATTTGGACAAGGGTGAAAGCAG.ATCCCGGTTTGCTTTTCCCCTTGGCGAAAATACCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                            <  1:1012671/101‑1 (MQ=21)
                                                           tCA.CCCCCCGGGATATTTGGACCAAGGTGAAGGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                           >  2:2097362/1‑101 (MQ=255)
                                                            cccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGGGTCCCGCAGGGACGGGGGGCag                                           >  2:3745347/4‑101 (MQ=18)
                                                             a.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                         <  1:3180309/101‑1 (MQ=255)
                                                              cccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                         <  1:267058/100‑1 (MQ=255)
                                                               ccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                        <  1:2484539/101‑1 (MQ=18)
                                                               ccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                        >  2:2244929/1‑101 (MQ=18)
                                                                cccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                       >  1:2758194/1‑101 (MQ=18)
                                                                cccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                       >  1:102579/1‑101 (MQ=18)
                                                                cccccGGGATATTTGGCCCAAGGCGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                       >  2:4338588/1‑101 (MQ=18)
                                                                 ccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAggcc                                      >  2:1614622/1‑101 (MQ=255)
                                                                  cccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccc                                     <  1:1638962/101‑1 (MQ=255)
                                                                   ccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccc                                    >  2:2337334/1‑101 (MQ=17)
                                                                    cGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                   <  1:3729870/101‑1 (MQ=17)
                                                                    cGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccc                                   <  1:4483109/101‑1 (MQ=255)
                                                                     gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                  <  2:744798/101‑1 (MQ=255)
                                                                      ggATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                 <  2:3858571/101‑3 (MQ=17)
                                                                        atatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                               <  2:1196117/101‑5 (MQ=17)
                                                                        atatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc                               >  1:474038/1‑101 (MQ=255)
                                                                        atatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc                               >  2:3501376/1‑101 (MQ=255)
                                                                         tatTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                              >  1:2452594/1‑96 (MQ=18)
                                                                         tatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                              <  2:1680358/101‑6 (MQ=17)
                                                                         tatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                              >  1:4658279/1‑101 (MQ=255)
                                                                         tatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                              >  2:3155551/1‑101 (MQ=255)
                                                                          atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCCGGGGCAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                             >  2:1886475/1‑101 (MQ=255)
                                                                            ttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                           <  1:75970/101‑1 (MQ=255)
                                                                            ttGGACCAAGGTGAAAGCAG.AGCCCGGTGTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                           <  1:1151881/101‑1 (MQ=255)
                                                                              ggACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                         >  2:3033763/1‑101 (MQ=255)
                                                                              ggACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                         >  1:385777/1‑101 (MQ=255)
                                                                                 ccAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgggg                      <  1:4126745/101‑1 (MQ=255)
                                                                                  cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                     >  1:555074/1‑101 (MQ=255)
                                                                                  cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCCGCCCCCCTGGGGc                     >  1:1121768/1‑101 (MQ=255)
                                                                                    aGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                   >  1:1311826/1‑101 (MQ=255)
                                                                                     ggTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                  <  2:2761617/101‑1 (MQ=255)
                                                                                     ggTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                  >  1:3142866/1‑101 (MQ=255)
                                                                                        gAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcg               >  1:1731962/1‑101 (MQ=255)
                                                                                         aaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc              >  2:4670203/1‑101 (MQ=255)
                                                                                           aGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTc            <  2:3194744/101‑1 (MQ=255)
                                                                                            gCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCa           <  2:120396/101‑1 (MQ=255)
                                                                                              aG.ATCCCGGATTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGAGCAGAGCCCCCCTGCCCCCCTGGTGCCGACCGCTCAgg         >  2:4552435/1‑101 (MQ=37)
                                                                                               g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc        <  1:4610843/101‑1 (MQ=255)
                                                                                                   cccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCGGCGGGGCCGGGGGGCGGAGCCCCCCTCCCCCCCTGGGGCCGGGCGCCCGGGCggt     >  2:263916/1‑99 (MQ=25)
                                                                                                   cccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtg     >  2:776449/1‑101 (MQ=255)
                                                                                                      ggTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGttt  <  2:493028/101‑1 (MQ=255)
                                                                                                      |                                                                                                    
CGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTT  >  NC_014034/3003941‑3004141

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG