Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,043 T→G 9.3% intergenic (+89/+87) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0430T→G 9.3% 51.5 ‑0.4 ‑0.6228intergenic (+89/+87)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (12/9):  ref base (105/99):  total (120/108)
Fisher's exact test strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.56e-01

Only 100 of 259 total aligned reads displayed.

GGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGG  >  NC_014034/3003943‑3004143
                                                                                                      |                                                                                                    
 gCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGtt                                                                                                     >  2:247885/1‑101 (MQ=255)
  ggagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGttt                                                                                                    <  1:3420506/100‑1 (MQ=255)
  ggagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGttt                                                                                                    <  1:3749906/100‑1 (MQ=255)
  cGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCGATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGttt                                                                                                    <  2:2604227/101‑1 (MQ=255)
   gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTg                                                                                                   <  1:2415615/101‑1 (MQ=255)
     gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                                                 >  1:3199351/1‑101 (MQ=255)
     gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                                                 >  1:3175755/1‑101 (MQ=255)
     gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGGGAAAGCAG.ATCCCGGTTTGcc                                                                                                 >  1:68448/1‑101 (MQ=255)
      aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCt                                                                                                <  1:2987196/101‑1 (MQ=255)
      aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCt                                                                                                <  2:3646444/101‑1 (MQ=255)
       tCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACTGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCtt                                                                                               <  2:3943887/101‑1 (MQ=255)
         tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                                             >  1:4414958/1‑101 (MQ=255)
         tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                                             <  2:2091994/101‑1 (MQ=255)
          gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                                            <  2:1100032/101‑1 (MQ=255)
             ggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCt                                                                                         <  1:3986657/101‑1 (MQ=255)
               cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTg                                                                                       >  1:522414/1‑101 (MQ=255)
                aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTgg                                                                                      >  2:2781154/1‑101 (MQ=255)
                 aGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGAAG.ATCCCGGTTTGCCTTTCACCTTGgc                                                                                     >  1:1679993/1‑101 (MQ=255)
                   gCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGgcgc                                                                                   <  1:2143489/101‑1 (MQ=255)
                    cGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCa                                                                                  >  1:643338/1‑101 (MQ=255)
                      gggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCaaa                                                                                <  1:4511483/101‑1 (MQ=255)
                       ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                               <  1:4463576/101‑1 (MQ=255)
                       ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                               <  2:1516039/101‑1 (MQ=255)
                       gaggCGCTGCCCCCCTCTGGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGCCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                               <  1:1144067/99‑1 (MQ=255)
                        gggCGCTGCCCCCCTCTTGGCCCGACGGCCAATTCACCCCCCCGGGAAATTTGGCCCCAGGGAAAAATAA.AACCGGGTTGTCCTTTTCCCTTTGCCCaaaa                                                                               >  1:4038576/1‑100 (MQ=12)
                              tGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgg                                                                        >  2:2078309/1‑101 (MQ=255)
                              tGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgg                                                                        >  1:2318857/1‑101 (MQ=255)
                               gCCCCCCTCTTGGCCTGACGGGCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                       >  1:2697277/1‑101 (MQ=255)
                               gCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                       >  1:4295272/1‑101 (MQ=255)
                               gCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                       >  2:1128896/1‑101 (MQ=255)
                                ccccccTCTTGGCCT.TCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGAT.AAAGAAG.GTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagccc                                                                >  2:1846795/1‑96 (MQ=37)
                                ccccccTCTTGGCCT.TCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGAT.AAAGAAG.GTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagccc                                                                <  1:2835562/101‑6 (MQ=37)
                                     ctctTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGCCGCAAATATCCCGGGGGGAGt                                                                 <  1:3494056/101‑1 (MQ=255)
                                      tctTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTc                                                                >  1:2608734/1‑101 (MQ=255)
                                        ttGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTccc                                                              >  2:476430/1‑101 (MQ=255)
                                         tGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                             >  1:2874867/1‑101 (MQ=255)
                                          ggCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                            >  2:920737/1‑101 (MQ=255)
                                          ggCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                            >  2:198119/1‑101 (MQ=255)
                                               gACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGa                                                       <  2:2402087/101‑1 (MQ=255)
                                                    ccAATTCC.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggg                                                  <  1:1460003/101‑1 (MQ=255)
                                                    ccAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggg                                                  >  2:4507949/1‑101 (MQ=255)
                                                     cAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                                                 >  2:4674720/1‑101 (MQ=255)
                                                     cAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                                                 <  2:2679205/101‑1 (MQ=255)
                                                      gcttCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                <  2:2144979/99‑1 (MQ=255)
                                                      aaTTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAGgggggg                                                >  1:3688875/1‑101 (MQ=255)
                                                       aTTCG.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.GTCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                               <  2:4409774/101‑1 (MQ=255)
                                                       aTTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                               <  2:4392376/101‑1 (MQ=255)
                                                       aTTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                               <  2:2729936/101‑1 (MQ=255)
                                                       aTTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCCGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGGGGCCCGCGGGGGCggggggg                                               >  2:4024223/1‑100 (MQ=21)
                                                        ttCC.CCCCCCGGGATATTTGGACAAGGGTGAAAGCAG.ATCCCGGTTTGCTTTTCCCCTTGGCGAAAATACCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                              <  1:1012671/101‑1 (MQ=21)
                                                          tccccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTGTGCCTTTCCCGTTGGCGGAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                             <  1:1266961/98‑1 (MQ=18)
                                                          cccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGGGTCCCGCAGGGACGGGGGGCag                                             >  2:3745347/4‑101 (MQ=18)
                                                          cA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                            >  1:1480378/1‑101 (MQ=255)
                                                           ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                            <  2:2893325/99‑2 (MQ=18)
                                                            cccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                           <  1:267058/100‑1 (MQ=255)
                                                             ccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                          <  1:2484539/101‑1 (MQ=18)
                                                              cccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                         >  1:2758194/1‑101 (MQ=18)
                                                              cccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                         >  1:102579/1‑101 (MQ=18)
                                                              cccccGGGATATTTGGCCCAAGGCGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                         >  2:4338588/1‑101 (MQ=18)
                                                               ccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAggcc                                        >  2:1614622/1‑101 (MQ=255)
                                                                  cGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccc                                     <  1:4483109/101‑1 (MQ=255)
                                                                  cGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccc                                     <  2:3175555/101‑1 (MQ=255)
                                                                   gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                    <  2:744798/101‑1 (MQ=255)
                                                                   gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                    >  1:3824660/1‑101 (MQ=255)
                                                                    ggATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                   <  2:3858571/101‑3 (MQ=17)
                                                                     gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                  >  1:220190/1‑101 (MQ=255)
                                                                     gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                  >  2:2769313/1‑101 (MQ=255)
                                                                     gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGAGCAGAgcccacctgc                                  >  1:4614400/1‑101 (MQ=255)
                                                                      atatTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                 >  2:2282405/1‑97 (MQ=18)
                                                                      atatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                 <  2:1196117/101‑5 (MQ=17)
                                                                      atatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc                                 >  1:474038/1‑101 (MQ=255)
                                                                       tttttGGCCAAAGGTGAAAGCAG.ATCCCGTTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                                <  2:3271633/99‑1 (MQ=255)
                                                                       tatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                                <  2:1680358/101‑6 (MQ=17)
                                                                        atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCCGGGGCAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                               >  2:1886475/1‑101 (MQ=255)
                                                                         tttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccccc                              <  1:3231582/101‑1 (MQ=255)
                                                                          ttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                             <  1:75970/101‑1 (MQ=255)
                                                                           tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                            <  1:247874/101‑1 (MQ=255)
                                                                           tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                            >  2:2957064/1‑101 (MQ=255)
                                                                           tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                            <  1:2918410/101‑1 (MQ=255)
                                                                            ggACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                           >  2:3033763/1‑101 (MQ=255)
                                                                               ccAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgggg                        <  1:4126745/101‑1 (MQ=255)
                                                                                cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCGCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                       <  2:4131107/101‑1 (MQ=255)
                                                                                cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                       >  1:555074/1‑101 (MQ=255)
                                                                                 aaGGTGAAAGCAG.ATCCCGTTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                      <  2:252846/101‑1 (MQ=255)
                                                                                   ggTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                    >  1:3142866/1‑101 (MQ=255)
                                                                                   ggTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                    <  1:1185718/101‑1 (MQ=255)
                                                                                    gTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                   <  2:510336/101‑1 (MQ=255)
                                                                                      gAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcg                 >  1:1731962/1‑101 (MQ=255)
                                                                                          gCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCa             <  1:198144/101‑1 (MQ=255)
                                                                                          gCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCa             <  2:120396/101‑1 (MQ=255)
                                                                                            aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg           >  2:2359562/1‑101 (MQ=255)
                                                                                            aG.ATCCCGGATTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGAGCAGAGCCCCCCTGCCCCCCTGGTGCCGACCGCTCAgg           >  2:4552435/1‑101 (MQ=37)
                                                                                             g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc          <  1:4610843/101‑1 (MQ=255)
                                                                                                tCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgt        >  2:3517660/1‑101 (MQ=255)
                                                                                                 cccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtg       >  2:776449/1‑101 (MQ=255)
                                                                                                  ccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtgt      >  1:4164827/1‑101 (MQ=255)
                                                                                                   cGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt     >  2:3465881/1‑101 (MQ=255)
                                                                                                    ggTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGttt    >  2:3090006/1‑101 (MQ=255)
                                                                                                     gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg   <  2:3621047/101‑1 (MQ=255)
                                                                                                      tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg  >  1:3623377/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
GGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGG  >  NC_014034/3003943‑3004143

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG