BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,004,048	C→A	9.1%	intergenic (+94/+82)	gfo → / ← lrp	glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,004,048	0	C→A	9.1%	45.7 0.0 ‑0.0	230	intergenic (+94/+82)	gfo/lrp	glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand): new base (12/9): ref base (115/94): total (127/103)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.34e-01

Only 100 of 258 total aligned reads displayed.


GATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGC  >  NC_014034/3003948‑3004148


gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCC.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                                                      <  1:2736103/101‑1 (MQ=255)


gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                                                      >  1:3199351/1‑101 (MQ=255)


gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                                                      >  1:3175755/1‑101 (MQ=255)


gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                                                      <  1:1465239/101‑1 (MQ=255)


 aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCt                                                                                                     <  1:2987196/101‑1 (MQ=255)


  tCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCtt                                                                                                    <  1:1607497/101‑1 (MQ=255)


   cTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCttt                                                                                                   >  1:685404/1‑101 (MQ=255)


    tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                                                  <  1:1719218/101‑1 (MQ=255)


    tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                                                  <  2:2091994/101‑1 (MQ=255)


    tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                                                  <  2:2119793/101‑1 (MQ=255)


     gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                                                 <  2:1100032/101‑1 (MQ=255)


       gggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCAcc                                                                                               >  2:2895738/1‑101 (MQ=255)


        ggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCt                                                                                              <  1:3986657/101‑1 (MQ=255)


        ggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCt                                                                                              <  1:2959037/101‑1 (MQ=255)


         gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCAGCtt                                                                                             <  1:4235841/101‑1 (MQ=255)


         gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCtt                                                                                             <  2:4733040/101‑1 (MQ=255)


          cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTg                                                                                            >  1:522414/1‑101 (MQ=255)


          cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTg                                                                                            <  1:342625/101‑1 (MQ=255)


          cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTg                                                                                            >  2:2697141/1‑101 (MQ=255)


           aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTgg                                                                                           >  2:2781154/1‑101 (MQ=255)


              gCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGgcgc                                                                                        <  1:2143489/101‑1 (MQ=255)


               cGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCa                                                                                       >  1:643338/1‑101 (MQ=255)


                ggggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCCCCTTGGCGCaa                                                                                      >  2:3619322/1‑101 (MQ=255)


                  ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                                    <  1:4463576/101‑1 (MQ=255)


                  ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                                    <  2:3057652/101‑1 (MQ=255)


                  ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                                    <  1:3623501/101‑1 (MQ=255)


                  ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                                    >  1:2679359/1‑101 (MQ=255)


                  gaggCGCTGCCCCCCTCTGGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGCCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                                    <  1:1144067/99‑1 (MQ=255)


                   gggCGCTGCCCCCCTCTTGGCCCGACGGCCAATTCACCCCCCCGGGAAATTTGGCCCCAGGGAAAAATAA.AACCGGGTTGTCCTTTTCCCTTTGCCCaaaa                                                                                    >  1:4038576/1‑100 (MQ=12)


                    ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                                  <  1:2606611/101‑1 (MQ=255)


                    ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                                  <  1:1966368/101‑1 (MQ=255)


                    ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTC..CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATc                                                                                 <  2:2495297/101‑1 (MQ=255)


                      cgcTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATcc                                                                                >  1:2210682/1‑101 (MQ=255)


                         tGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgg                                                                             <  1:3242623/101‑1 (MQ=255)


                         tGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgg                                                                             >  1:2318857/1‑101 (MQ=255)


                         tGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgg                                                                             >  2:2078309/1‑101 (MQ=255)


                          gCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                            >  2:1128896/1‑101 (MQ=255)


                           ccccccTCTTGGCCT.TCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGAT.AAAGAAG.GTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagccc                                                                     >  2:1846795/1‑96 (MQ=37)


                           ccccccTCTTGGCCT.TCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGAT.AAAGAAG.GTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagccc                                                                     <  1:2835562/101‑6 (MQ=37)


                            cccccTCTTGGCCTGACGGCCAATTCC.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggggg                                                                          <  2:3644218/101‑1 (MQ=255)


                                ctctTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGCTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGt                                                                      >  2:1438952/1‑101 (MQ=255)


                                 tctTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTc                                                                     >  2:2621312/1‑101 (MQ=255)


                                 tctTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTc                                                                     >  1:563196/1‑101 (MQ=255)


                                    tGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                                  >  2:1610889/1‑101 (MQ=255)


                                    tGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                                  <  2:4348233/101‑1 (MQ=255)


                                    tGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                                  >  1:2874867/1‑101 (MQ=255)


                                     ggCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                                 >  2:198119/1‑101 (MQ=255)


                                     ggCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                                 >  2:920737/1‑101 (MQ=255)


                                         tGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                             <  2:4207429/101‑1 (MQ=255)


                                          gACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGa                                                            >  2:1197034/1‑101 (MQ=255)


                                          gACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGa                                                            <  2:2402087/101‑1 (MQ=255)


                                           aCGGCCAATTCC.CCCCCCGGGATATTTGGCCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                                                           <  2:673217/101‑1 (MQ=255)


                                                 aaTTCA.CCCCCCGGGATATTTGGTCGAAGGAGAAAGCAG.ATCCCGGATTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                     >  2:1528381/1‑101 (MQ=25)


                                                 aaTTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                     >  1:2087249/1‑101 (MQ=255)


                                                  aTTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                    >  2:3373161/1‑101 (MQ=255)


                                                  aTTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                    >  2:2733010/1‑101 (MQ=255)


                                                  aTTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                    <  2:4392376/101‑1 (MQ=255)


                                                   ttCC.CCCCCCGGGATATTTGGACAAGGGTGAAAGCAG.ATCCCGGTTTGCTTTTCCCCTTGGCGAAAATACCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                                   <  1:1012671/101‑1 (MQ=21)


                                                       cccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                                <  2:3933354/100‑1 (MQ=255)


                                                        ccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                               <  1:2484539/101‑1 (MQ=18)


                                                         cccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                              >  1:102579/1‑101 (MQ=18)


                                                             cGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                          <  1:3729870/101‑1 (MQ=17)


                                                             cGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccc                                          <  1:4483109/101‑1 (MQ=255)


                                                              gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                         >  1:3824660/1‑101 (MQ=255)


                                                              gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                         >  2:4440822/1‑101 (MQ=255)


                                                              gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                         <  2:744798/101‑1 (MQ=255)


                                                               ggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctg                                        <  2:3688645/101‑1 (MQ=255)


                                                                 atatTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                      >  2:2282405/1‑97 (MQ=18)


                                                                 atatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc                                      >  2:3501376/1‑101 (MQ=255)


                                                                 atatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc                                      >  1:474038/1‑101 (MQ=255)


                                                                  tatTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                                     >  1:2452594/1‑96 (MQ=18)


                                                                  tatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                                     <  2:3997574/101‑6 (MQ=17)


                                                                  tatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                                     <  2:1680358/101‑6 (MQ=17)


                                                                  tatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                                     >  1:4658279/1‑101 (MQ=255)


                                                                  tatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                                     >  2:3155551/1‑101 (MQ=255)


                                                                   atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCCGGGGCAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                    >  2:1886475/1‑101 (MQ=255)


                                                                     ttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                                  <  1:75970/101‑1 (MQ=255)


                                                                     ttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                                  >  1:4351023/1‑101 (MQ=255)


                                                                      tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                                 >  2:2776229/1‑101 (MQ=255)


                                                                      tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                                 >  2:2957064/1‑101 (MQ=255)


                                                                       ggACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                                >  1:4602673/1‑101 (MQ=255)


                                                                            aaGGTGAAAGCAG.ATCCCGTTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                           <  2:252846/101‑1 (MQ=255)


                                                                             aGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                          >  1:1311826/1‑101 (MQ=255)


                                                                             aGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                          <  2:3788965/101‑1 (MQ=255)


                                                                              ggTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                         >  1:3142866/1‑101 (MQ=255)


                                                                              ggTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                         <  1:1185718/101‑1 (MQ=255)


                                                                                  aaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc                     >  2:4670203/1‑101 (MQ=255)


                                                                                   aaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt                    >  1:4092223/1‑101 (MQ=255)


                                                                                    aGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTc                   <  2:3194744/101‑1 (MQ=255)


                                                                                       aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                >  2:2359562/1‑101 (MQ=255)


                                                                                       aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                >  2:4122377/1‑101 (MQ=255)


                                                                                       aG.ATCCCGGATTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGAGCAGAGCCCCCCTGCCCCCCTGGTGCCGACCGCTCAgg                >  2:4552435/1‑101 (MQ=37)


                                                                                        g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc               <  1:4610843/101‑1 (MQ=255)


                                                                                          aTCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCg              >  1:3533339/1‑101 (MQ=255)


                                                                                            cccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtg            >  2:776449/1‑101 (MQ=255)


                                                                                             ccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtgt           >  1:4164827/1‑101 (MQ=255)


                                                                                               ggTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGttt         <  2:493028/101‑1 (MQ=255)


                                                                                                 tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg       >  1:2647264/1‑101 (MQ=255)


                                                                                                    gCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGa    >  1:94968/1‑101 (MQ=255)


                                                                                                     ccTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTGTGGCggg   >  2:4412821/1‑99 (MQ=255)


GATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGC  >  NC_014034/3003948‑3004148

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG