Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,059 G→C 2.3% intergenic (+105/+71) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0590G→C 2.3% 2.4 ‑1.0 ‑0.0211intergenic (+105/+71)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (2/3):  ref base (126/80):  total (128/83)
Fisher's exact test strand distribution p-value = 3.84e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.96e-01

Only 100 of 241 total aligned reads displayed.

AAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCG  >  NC_014034/3003959‑3004157
                                                                                                    |                                                                                                  
aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGCGGTATTTGGCCCCAGGGGAAAGCAGATCCCGGTTTGCCTTTCACCTTgg                                                                                                    >  1:2761781/1‑101 (MQ=37)
aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCCCACGGCCAATTCACCCCCCCGCAAATTTTGGCCAAGGGGGGAGCAGACCCCGGTTTGCCTTTCCCCTTgg                                                                                                    >  1:2415385/1‑101 (MQ=21)
 aGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGAAGATCCCGGTTTGCCTTTCACCTTGgc                                                                                                   >  1:1679993/1‑101 (MQ=255)
  tgCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGgcg                                                                                                  <  1:4295842/100‑1 (MQ=255)
  ggCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGgcg                                                                                                  >  2:282998/1‑101 (MQ=255)
   gCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGgcgc                                                                                                 >  2:2780868/1‑101 (MQ=255)
    cGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCa                                                                                                >  1:643338/1‑101 (MQ=255)
      gggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCaaa                                                                                              <  1:4511483/101‑1 (MQ=255)
       ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                                             <  2:146833/101‑1 (MQ=255)
       ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                                             >  1:2679359/1‑101 (MQ=255)
         ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                                           <  1:219207/101‑1 (MQ=255)
           cgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATcc                                                                                         >  1:2210682/1‑101 (MQ=255)
              tGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgg                                                                                      >  2:2078309/1‑101 (MQ=255)
               gCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                                     >  2:1128896/1‑101 (MQ=255)
                ccccccTCTTGGCCT.TCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGAT.AAAGAAGGTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagccc                                                                              >  2:3977735/1‑96 (MQ=37)
                 cccccTCTTGGCCT.TCGGCCAATTCCCCCCCCGGGATATTTCGGGCAAGAT.AAAGAAGGTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagcccg                                                                             <  1:287408/101‑7 (MQ=37)
                      tctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTc                                                                              >  1:2608734/1‑101 (MQ=255)
                      tctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTc                                                                              >  1:563196/1‑101 (MQ=255)
                        ttGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTccc                                                                            >  2:751411/1‑101 (MQ=255)
                        ttGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTccc                                                                            >  2:476430/1‑101 (MQ=255)
                         tGGCCTGACGGCCAATTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                                           <  1:402429/101‑1 (MQ=255)
                          ggCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                                          >  2:920737/1‑101 (MQ=255)
                          ggCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                                          >  1:406093/1‑101 (MQ=255)
                          ggCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                                          >  2:198119/1‑101 (MQ=255)
                              tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                                      <  2:4207429/101‑1 (MQ=255)
                               gACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGa                                                                     >  2:1197034/1‑101 (MQ=255)
                               gACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGa                                                                     <  2:2402087/101‑1 (MQ=255)
                                aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                                                                    <  1:2724240/101‑1 (MQ=255)
                                  ggCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgg                                                                  >  1:1468305/1‑101 (MQ=255)
                                      aaTTCACCCCCCGGGATATTTGGTCGAAGGAGAAAGCAGATCCCGGATTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                              >  2:1528381/1‑101 (MQ=25)
                                      aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                              >  1:2087249/1‑101 (MQ=255)
                                       aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                             <  2:4392376/101‑1 (MQ=255)
                                       aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                             <  2:2729936/101‑1 (MQ=255)
                                       aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                             >  2:3373161/1‑101 (MQ=255)
                                        ttCCCCCCCCGGGATATTTGGACAAGGGTGAAAGCAGATCCCGGTTTGCTTTTCCCCTTGGCGAAAATACCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                                            <  1:1012671/101‑1 (MQ=21)
                                         tccccccccGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTGTGCCTTTCCCGTTGGCGGAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                                           <  1:1266961/98‑1 (MQ=18)
                                         tCACCCCCCGGGATATTTGGACCAAGGTGAAGGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                                           >  2:2097362/1‑101 (MQ=255)
                                         tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                                           <  1:368730/101‑1 (MQ=255)
                                          ccccccccGGGATATTTGGCCCAAGGTGAAATCCCCCCCCCGTGTTCATTTCCCCTTGGCGGAAATATCCCGGTGGGAGTCCCGCAGGGACGGGGGGCagc                                                          <  1:460664/99‑2 (MQ=17)
                                           aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                                         <  1:3180309/101‑1 (MQ=255)
                                            ccccccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                                        <  1:2484539/101‑1 (MQ=18)
                                            ccccccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                                        >  2:2244929/1‑101 (MQ=18)
                                             cccccGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                                       >  1:102579/1‑101 (MQ=18)
                                             cccccGGGATATTTGGCCCAAGGCGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                                       >  2:4338588/1‑101 (MQ=18)
                                              ccccGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAggcc                                                      >  2:1614622/1‑101 (MQ=255)
                                                 cGGGATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                                   <  1:3729870/101‑1 (MQ=17)
                                                 cGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccc                                                   <  1:4483109/101‑1 (MQ=255)
                                                 cGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccc                                                   <  2:3175555/101‑1 (MQ=255)
                                                  gggATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCTCGGGGGGAGTCCCGCGGGGGCGGGGGGCAGAgcccccct                                                  <  1:502862/101‑1 (MQ=255)
                                                    gATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctc                                                >  2:2498202/1‑98 (MQ=18)
                                                    gATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                                >  2:2769313/1‑101 (MQ=255)
                                                    gATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGAGCAGAgcccacctgc                                                >  1:4614400/1‑101 (MQ=255)
                                                     atatTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc                                               >  1:474038/1‑101 (MQ=255)
                                                      tttttGGCCAAAGGTGAAAGCAGATCCCGTTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                                              <  2:3271633/99‑1 (MQ=255)
                                                      tatTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                                              <  2:1680358/101‑6 (MQ=17)
                                                      tatTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                                              >  1:4658279/1‑101 (MQ=255)
                                                       atTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCCGGGGCAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                             >  2:1886475/1‑101 (MQ=255)
                                                        tttGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccccc                                            <  1:3231582/101‑1 (MQ=255)
                                                         ttGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                                           >  1:2885002/1‑101 (MQ=255)
                                                         ttGGACCAAGGTGAAAGCAGAGCCCGGTGTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                                           <  1:1151881/101‑1 (MQ=255)
                                                          tGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                                          <  1:247874/101‑1 (MQ=255)
                                                           ggACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                                         >  2:3033763/1‑101 (MQ=255)
                                                           ggACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                                         >  1:385777/1‑101 (MQ=255)
                                                              ccAAGGTGAAAGCAGATCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgggg                                      <  1:4126745/101‑1 (MQ=255)
                                                              ccAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgggg                                      >  2:24176/1‑101 (MQ=255)
                                                               cAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                                     >  1:3487931/1‑101 (MQ=255)
                                                               cAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCCGCCCCCCTGGGGc                                     >  1:1121768/1‑101 (MQ=255)
                                                                aaGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                                    <  1:3114674/101‑1 (MQ=255)
                                                                 aGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                                   <  2:3788965/101‑1 (MQ=255)
                                                                  ggTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                                  >  1:3142866/1‑101 (MQ=255)
                                                                   gTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                                 <  2:510336/101‑1 (MQ=255)
                                                                      aaaGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc                              >  2:4670203/1‑101 (MQ=255)
                                                                         gCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCa                           <  1:198144/101‑1 (MQ=255)
                                                                           aGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                         >  2:2359562/1‑101 (MQ=255)
                                                                           aGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                         >  2:4122377/1‑101 (MQ=255)
                                                                           aGATCCCGGATTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGAGCAGAGCCCCCCTGCCCCCCTGGTGCCGACCGCTCAgg                         >  2:4552435/1‑101 (MQ=37)
                                                                             aTCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCg                       >  2:1696853/1‑101 (MQ=255)
                                                                              tCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgt                      >  2:3517660/1‑101 (MQ=255)
                                                                               cccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCGGCGGGGCCGGGGGGCGGAGCCCCCCTCCCCCCCTGGGGCCGGGCGCCCGGGCggt                     >  2:263916/1‑99 (MQ=25)
                                                                                ccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtgt                    >  1:4164827/1‑101 (MQ=255)
                                                                                  ggTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGttt                  <  1:729592/101‑1 (MQ=255)
                                                                                   gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg                 <  2:3621047/101‑1 (MQ=255)
                                                                                    tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg                >  2:74294/1‑101 (MQ=255)
                                                                                    tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg                >  1:2647264/1‑101 (MQ=255)
                                                                                    tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg                >  1:3623377/1‑101 (MQ=255)
                                                                                     ttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGCGTTTGGc               >  2:2545404/1‑101 (MQ=255)
                                                                                       gCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGa             >  2:3700706/1‑101 (MQ=255)
                                                                                        ccTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAg            <  1:920814/101‑1 (MQ=255)
                                                                                        ccTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTGTGGCggg            >  2:4412821/1‑99 (MQ=255)
                                                                                         cTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGc           >  1:951424/1‑101 (MQ=255)
                                                                                           ttCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCg         >  2:4014586/1‑101 (MQ=255)
                                                                                            tCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGc        >  2:797208/1‑101 (MQ=255)
                                                                                             cACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAGGGGGGGCAGACCCCCCCTGCCCCCCTGGGGCCGAGGGCTCAGGCGTGTTTGGCGAGCCGct       <  2:3061422/101‑1 (MQ=255)
                                                                                             cACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGGAGAGCCCCCCCGCCCCCCCCGGGCCGCGCGCTCAGGCGGGTTTGGCGCGCCGcc       >  1:162873/1‑100 (MQ=25)
                                                                                               cgtgggCGAAAATTCCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGctct     <  2:4457899/97‑1 (MQ=37)
                                                                                               ccTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGGGCGCTCAGGCGTGTTTGGCGAGCCGctct     >  2:1171804/1‑101 (MQ=255)
                                                                                               ccTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGctct     >  1:4400122/1‑101 (MQ=255)
                                                                                                cTTGGCACAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCtt    >  2:1860986/1‑101 (MQ=255)
                                                                                                  tGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCg  <  2:563145/101‑1 (MQ=255)
                                                                                                  tGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCg  <  2:2318724/101‑1 (MQ=255)
                                                                                                    |                                                                                                  
AAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCG  >  NC_014034/3003959‑3004157

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG