Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,086 G→A 9.9% intergenic (+132/+44) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0860G→A 9.9% 49.2 ‑1.0 ‑0.2211intergenic (+132/+44)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (11/10):  ref base (119/71):  total (130/81)
Fisher's exact test strand distribution p-value = 3.57e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.09e-01

Only 100 of 233 total aligned reads displayed.

CCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGT  >  NC_014034/3003987‑3004185
                                                                                                    |                                                                                                   
  tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                                                                  >  1:4211298/1‑101 (MQ=255)
  tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                                                                  <  2:4207429/101‑1 (MQ=255)
   gACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGa                                                                                                 >  2:1197034/1‑101 (MQ=255)
    aCGGCCAATTCCCCCCCCGGGATATTTGGCCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                                                                                                <  2:673217/101‑1 (MQ=255)
     cGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACg                                                                                               <  1:312844/101‑1 (MQ=255)
         cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                                                                                           <  2:2679205/101‑1 (MQ=255)
          aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAGgggggg                                                                                          >  1:3688875/1‑101 (MQ=255)
          aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                                                          >  1:2087249/1‑101 (MQ=255)
           aTTCGCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.GTCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                                                         <  2:4409774/101‑1 (MQ=255)
           aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                                                         >  2:2733010/1‑101 (MQ=255)
           aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCCGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGGGGCCCGCGGGGGCggggggg                                                                                         >  2:4024223/1‑100 (MQ=21)
             tccccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTGTGCCTTTCCCGTTGGCGGAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                                                                       <  1:1266961/98‑1 (MQ=18)
              ccccccccGGGATATTTGGCCCAAGGTGAAATCCC.CCCCCCGTGTTCATTTCCCCTTGGCGGAAATATCCCGGTGGGAGTCCCGCAGGGACGGGGGGCagc                                                                                      <  1:460664/99‑2 (MQ=17)
              cACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGGCGGGGGGCaga                                                                                      >  1:4131353/1‑101 (MQ=255)
              cACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                                                                      >  1:1480378/1‑101 (MQ=255)
               aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                                                                     >  1:1076321/1‑101 (MQ=255)
               aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                                                                     <  1:3180309/101‑1 (MQ=255)
                ccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                                                                    >  2:2244929/1‑101 (MQ=18)
                 cccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                                                                   >  1:2758194/1‑101 (MQ=18)
                  ccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAggcc                                                                                  >  2:1614622/1‑101 (MQ=255)
                   cccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccc                                                                                 <  1:1638962/101‑1 (MQ=255)
                    ccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccc                                                                                >  2:2337334/1‑101 (MQ=17)
                      gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCTCGGGGGGAGTCCCGCGGGGGCGGGGGGCAGAgcccccct                                                                              <  1:502862/101‑1 (MQ=255)
                      gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                                                              <  2:744798/101‑1 (MQ=255)
                      gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                                                              >  1:3824660/1‑101 (MQ=255)
                       ggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctg                                                                             <  2:3688645/101‑1 (MQ=255)
                        gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                                                            >  2:2769313/1‑101 (MQ=255)
                        gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                                                            >  2:1103673/1‑101 (MQ=255)
                          tttttGGCCAAAGGTGAAAGCAG.ATCCCGTTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                                                                          <  2:3271633/99‑1 (MQ=255)
                          tatTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                                                                          >  1:2452594/1‑96 (MQ=18)
                          tatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                                                                          <  2:1680358/101‑6 (MQ=17)
                          tatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                                                                          >  2:3155551/1‑101 (MQ=255)
                           atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                                                         >  2:787827/1‑101 (MQ=255)
                           atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCCGGGGCAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                                                         >  2:1886475/1‑101 (MQ=255)
                              tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                                                                      <  1:2918410/101‑1 (MQ=255)
                                   cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCGCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                                                                 <  2:4131107/101‑1 (MQ=255)
                                   cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                                                                 >  1:3487931/1‑101 (MQ=255)
                                    aaGGTGAAAGCAG.ATCCCGTTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                                                                <  2:252846/101‑1 (MQ=255)
                                    aaGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                                                                <  1:3114674/101‑1 (MQ=255)
                                     aGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                                                               <  2:3788965/101‑1 (MQ=255)
                                       gTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                                                             <  2:510336/101‑1 (MQ=255)
                                        tGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgc                                                            >  1:2602509/1‑101 (MQ=255)
                                            aGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTc                                                        <  2:3194744/101‑1 (MQ=255)
                                              cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                                                      >  1:1019373/1‑101 (MQ=255)
                                               aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg                                                     >  2:2359562/1‑101 (MQ=255)
                                               aG.ATCCCGGATTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGAGCAGAGCCCCCCTGCCCCCCTGGTGCCGACCGCTCAgg                                                     >  2:4552435/1‑101 (MQ=37)
                                                  aTCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCg                                                   >  2:1696853/1‑101 (MQ=255)
                                                   tCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgt                                                  >  2:3517660/1‑101 (MQ=255)
                                                    cccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCCCGGGGCGGGGGGGCAG.GCCCCCCCGGCCCCCCGGGGCCGGGCGGCCAGGCggtg                                                >  1:4611739/1‑98 (MQ=21)
                                                      cGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt                                               >  2:3465881/1‑101 (MQ=255)
                                                       ggTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGttt                                              <  1:729592/101‑1 (MQ=255)
                                                        gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg                                             <  2:2087128/101‑1 (MQ=255)
                                                         tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg                                            >  1:3623377/1‑101 (MQ=255)
                                                         tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg                                            >  1:2647264/1‑101 (MQ=255)
                                                         tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg                                            >  2:25941/1‑101 (MQ=255)
                                                          ttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGc                                           <  2:685340/101‑1 (MQ=255)
                                                            gCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGa                                         >  1:94968/1‑101 (MQ=255)
                                                                ttCCCCTTGGCGCAAATCGCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCg                                     <  1:1124503/101‑1 (MQ=255)
                                                                 tcgccTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGc                                    >  1:2712065/4‑101 (MQ=255)
                                                                  ccccTTGGCGCAAATACCCGGGGGGGAGTCCCGCGGGGAGGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGct                                   <  2:2415250/99‑1 (MQ=255)
                                                                  cACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAGGGGGGGCAGACCCCCCCTGCCCCCCTGGGGCCGAGGGCTCAGGCGTGTTTGGCGAGCCGct                                   <  2:3061422/101‑1 (MQ=255)
                                                                   aCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGctc                                  >  1:2008221/1‑101 (MQ=255)
                                                                    ccTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGGGCGCTCAGGCGTGTTTGGCGAGCCGctct                                 >  2:1171804/1‑101 (MQ=255)
                                                                    ccTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGctct                                 >  1:4400122/1‑101 (MQ=255)
                                                                     ctggccgcAAATCCCCGGGGGGGAGCCCCGCGGGGAGGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCtt                                <  2:844226/96‑1 (MQ=255)
                                                                     cTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCtt                                >  1:4733313/1‑101 (MQ=255)
                                                                          ggcAAATACCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGc                           <  1:4553353/100‑1 (MQ=255)
                                                                          cgcAAATCTGCCGGGGGGAGTCCCGCGGGGCCGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTAAGGCGTGTTTGGCGAGCCGCTCTTCGAGc                           <  1:1439046/101‑1 (MQ=38)
                                                                          cgcAAATATCCGGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGc                           <  2:1945799/101‑1 (MQ=255)
                                                                           gcAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCa                          >  1:336558/1‑101 (MQ=255)
                                                                            cAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCAc                         >  1:1787290/1‑101 (MQ=255)
                                                                            cAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCAc                         <  1:2263717/101‑1 (MQ=255)
                                                                            cAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCAc                         >  1:3909102/1‑101 (MQ=255)
                                                                            cAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCAc                         >  2:3227555/1‑101 (MQ=255)
                                                                             aaaTATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGGCGCTCTTCGAGCACg                        >  2:2128946/1‑101 (MQ=255)
                                                                             aaaTATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACg                        >  2:1865228/1‑101 (MQ=255)
                                                                              cttccgatggggggGGCCCCGGGGGGCGGGGGGGCAGACCCCCCCTGCCCCCCTGGGGCCGAGCCCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGt                       <  1:1169315/93‑1 (MQ=255)
                                                                               atatCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGtg                      >  1:2140815/1‑100 (MQ=255)
                                                                               atatCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTc                      >  2:3995812/1‑101 (MQ=255)
                                                                               atatCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTc                      >  2:2984680/1‑101 (MQ=255)
                                                                                 atCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTTAGGCGTGTTTGGCGCGCCGGTCTTCGAGCACGTCgc                    >  2:4436247/1‑100 (MQ=255)
                                                                                 atCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGa                    >  1:4067877/1‑101 (MQ=255)
                                                                                  tCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGaa                   >  2:2806194/1‑101 (MQ=255)
                                                                                     cGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAggg                >  2:4719961/1‑101 (MQ=255)
                                                                                      ggggggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGc               >  1:1005805/1‑101 (MQ=255)
                                                                                      ggggggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGc               >  2:580847/1‑101 (MQ=255)
                                                                                      ggggggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGc               >  2:1406072/1‑101 (MQ=255)
                                                                                        ggggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTCCCCCCCTGGGGCCGGGCGCTCAGGCGTTTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCAc             <  1:247910/101‑1 (MQ=255)
                                                                                         gggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACg            >  1:4647330/1‑101 (MQ=255)
                                                                                          ggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGc           >  1:797260/1‑101 (MQ=255)
                                                                                            ggccccGGGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTTTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCg         <  1:4032640/98‑1 (MQ=255)
                                                                                             gTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCgg        >  2:482674/1‑101 (MQ=255)
                                                                                              tCCCGGGGGGAGGGGGGTCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCggg       <  1:2781033/101‑1 (MQ=255)
                                                                                              tCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCggg       >  1:581174/1‑101 (MQ=255)
                                                                                               cgggcgGGGGGGGGGGCCACCCCCCCCCGGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCgggg      <  2:4385963/98‑1 (MQ=255)
                                                                                                 cgcgGGGGCGGGGGGCAGACCCCCCCTGCCCCCCTGGGGCCGAGCGCTCGGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtc    <  1:2365/101‑1 (MQ=255)
                                                                                                 cgcgGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtc    >  2:4489510/1‑101 (MQ=255)
                                                                                                 cgcgGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtc    >  2:1019987/1‑101 (MQ=255)
                                                                                                  gcgGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcg   >  2:460412/1‑101 (MQ=255)
                                                                                                   cgGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcgt  >  2:3883739/1‑101 (MQ=255)
                                                                                                    |                                                                                                   
CCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGT  >  NC_014034/3003987‑3004185

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG