Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,011,287 A→T 79.8% intergenic (+86/+56) purK → / ← RCAP_rcc02822 phosphoribosylaminoimidazole carboxylase ATPase subunit/lipase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,011,2870A→T79.8% 149.9 ‑0.3 0.0218intergenic (+86/+56)purK/RCAP_rcc02822phosphoribosylaminoimidazole carboxylase ATPase subunit/lipase
Reads supporting (aligned to +/- strand):  new base (113/61):  ref base (27/17):  total (140/78)
Fisher's exact test strand distribution p-value = 7.25e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

TTCTCTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AATAGTCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGC  >  NC_014034/3011194‑3011380
                                                                                              |                                                                                             
ttCTCTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCgg                                                                                        >  1:3379463/1‑101 (MQ=255)
ttCTCTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCgg                                                                                        <  1:1699116/101‑1 (MQ=255)
 tctcTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCggg                                                                                       >  2:1922329/1‑101 (MQ=255)
 tctcTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCggg                                                                                       >  1:318782/1‑101 (MQ=255)
  ctctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCgggg                                                                                      >  1:1169665/1‑101 (MQ=255)
   tctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCggggg                                                                                     >  2:880067/1‑101 (MQ=255)
     tGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGga                                                                                   <  1:3751443/101‑1 (MQ=255)
     tGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGga                                                                                   >  1:4315016/1‑101 (MQ=255)
      gACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGgag                                                                                  <  1:4673637/101‑1 (MQ=255)
       gccGCGGCAGCGGGGGCTTTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAACCAACGCCCTTTCCCCTTGGGAA.AAATATCCCGGGGGgaga                                                                                 <  2:1493873/100‑1 (MQ=25)
       aCCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGgaga                                                                                 <  2:3295067/101‑1 (MQ=255)
         cgcgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACt                                                                               <  1:4411572/101‑1 (MQ=255)
         cgcgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACt                                                                               <  1:640766/101‑1 (MQ=255)
           cgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTg                                                                             >  2:2961732/1‑101 (MQ=255)
            ggCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGc                                                                            <  2:2449814/101‑1 (MQ=255)
               aGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGc                                                                         <  2:3406496/101‑1 (MQ=255)
               aGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGc                                                                         <  1:4703523/101‑1 (MQ=255)
                 cGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACttagatcgg                                                                       >  2:3789212/1‑94 (MQ=37)
                 cGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAg                                                                       >  2:2722512/1‑101 (MQ=255)
                 cGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAg                                                                       <  1:979530/101‑1 (MQ=255)
                  gggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAgg                                                                      >  1:3028534/1‑101 (MQ=255)
                  gggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAgg                                                                      >  1:3317021/1‑101 (MQ=255)
                  gggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAgg                                                                      >  1:3842875/1‑101 (MQ=255)
                   ggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGc                                                                     <  2:1774733/101‑1 (MQ=255)
                     ggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCaa                                                                   <  2:2103397/101‑1 (MQ=255)
                     ggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCaa                                                                   <  2:4543440/101‑1 (MQ=255)
                     ggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCaa                                                                   <  2:586645/101‑1 (MQ=255)
                      gCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAag                                                                  <  1:306641/101‑1 (MQ=255)
                       cTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAaga                                                                 >  2:4173425/1‑101 (MQ=255)
                       cTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAaga                                                                 >  2:4335863/1‑101 (MQ=255)
                        tGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAagag                                                                >  2:175295/1‑101 (MQ=255)
                         gTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAgg                                                               >  2:1287358/1‑101 (MQ=255)
                         gTCTGCCCCCGCACCCCCGAGGATATTTCCGACAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGTAGACTCGCCGCAGGCAAGAgg                                                               >  2:727701/1‑101 (MQ=38)
                             gCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACGACGCCCTTTCCCGTTGGGAC.AAATATCCCGGGGGAAGACTTGCCGCAGGCAAGAgggggg                                                           <  1:727774/101‑1 (MQ=37)
                              cccccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGc                                                          <  1:1848014/101‑1 (MQ=255)
                               ccccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCa                                                         <  1:1263974/101‑1 (MQ=255)
                               ccccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCa                                                         <  2:553904/101‑1 (MQ=255)
                               ccccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGCAGGGAGACTTGCCTCAGGCAAGAAGGGGGCa                                                         >  2:4263617/1‑101 (MQ=37)
                                cccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAg                                                        <  1:4360319/101‑1 (MQ=255)
                                  cGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAgcg                                                      <  1:4704671/101‑1 (MQ=255)
                                  cGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAgcg                                                      >  2:263891/1‑101 (MQ=255)
                                   gCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAgcgc                                                     >  2:851707/1‑101 (MQ=255)
                                   gCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAgcgc                                                     >  2:539702/1‑101 (MQ=255)
                                    cACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGcc                                                    >  2:671534/1‑101 (MQ=255)
                                    cACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGcc                                                    <  2:2556734/101‑1 (MQ=255)
                                      cccccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGcccc                                                  <  2:1552047/101‑1 (MQ=255)
                                        cccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGcccccc                                                >  2:2366749/1‑101 (MQ=255)
                                        cccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGcccccc                                                <  2:2199564/101‑1 (MQ=255)
                                         ccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCt                                               >  1:2087082/1‑101 (MQ=255)
                                           gAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGc                                             >  2:523551/1‑101 (MQ=255)
                                           gAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGc                                             >  2:3288491/1‑101 (MQ=255)
                                           gAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGc                                             >  2:3106314/1‑101 (MQ=255)
                                            aGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGcc                                            >  1:3550106/1‑101 (MQ=255)
                                             ggATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGccc                                           <  2:3995994/101‑1 (MQ=255)
                                              gATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCg                                          >  1:791157/1‑101 (MQ=255)
                                               atatTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCgg                                         <  2:3047534/101‑1 (MQ=255)
                                                tatTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGc                                        >  2:502266/1‑101 (MQ=255)
                                                tatTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGc                                        <  2:1017650/101‑1 (MQ=255)
                                                 atTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGCCTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGcc                                       >  2:1128466/1‑100 (MQ=37)
                                                 atTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCt                                       >  1:2359392/1‑101 (MQ=255)
                                                 atTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCt                                       >  1:3083493/1‑101 (MQ=255)
                                                   ttCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCa                                     <  1:1922441/101‑1 (MQ=255)
                                                   ttCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCa                                     >  2:366561/1‑101 (MQ=255)
                                                    tCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCAc                                    >  2:3505475/1‑101 (MQ=255)
                                                      cGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACgg                                  >  1:965037/1‑101 (MQ=255)
                                                      cGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACgg                                  <  1:1148692/101‑1 (MQ=255)
                                                        ccAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCa                                <  1:2585764/101‑1 (MQ=255)
                                                         cAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAg                               <  1:1532162/101‑1 (MQ=255)
                                                         cAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAg                               <  2:3673482/101‑1 (MQ=255)
                                                          aaGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCGGCCCGGCTCACGGCAgg                              >  2:4483619/1‑101 (MQ=25)
                                                            ggTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGc                            <  1:1329132/101‑1 (MQ=255)
                                                              tGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCa                          >  1:2124636/1‑101 (MQ=255)
                                                               gAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGGCAg                         >  2:1238847/1‑101 (MQ=37)
                                                               gAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAg                         >  1:4752845/1‑101 (MQ=255)
                                                                 aaaCAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGcc                       >  2:62756/1‑101 (MQ=255)
                                                                 aaaCAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGcc                       >  1:4558063/1‑101 (MQ=255)
                                                                  aacaacGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCg                      >  1:2408634/1‑101 (MQ=255)
                                                                  aacaacGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCg                      <  1:866882/101‑1 (MQ=255)
                                                                    caacGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGcg                    >  2:3559016/1‑100 (MQ=37)
                                                                       cGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCCCGGCAGGGCCAGCCGCTGCa                 >  1:2758700/1‑101 (MQ=25)
                                                                       cGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCa                 <  1:2979370/101‑1 (MQ=255)
                                                                        gCCCTTTCCCCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCaa                <  1:2029234/101‑1 (MQ=37)
                                                                        gCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCaa                >  1:2928263/1‑101 (MQ=255)
                                                                        gCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCaa                >  1:4220862/1‑101 (MQ=255)
                                                                        gCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCaa                >  1:3164288/1‑101 (MQ=255)
                                                                         cccTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCTAGCCGCTGCAAg               >  1:843894/1‑101 (MQ=37)
                                                                          ccTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAgg              >  2:2401556/1‑101 (MQ=255)
                                                                            tttCCCCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGcc            <  2:2660642/101‑1 (MQ=37)
                                                                            tttCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGcc            >  1:2488552/1‑101 (MQ=255)
                                                                             ttCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCcg           >  2:4351636/1‑101 (MQ=255)
                                                                             ttCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCcg           >  1:2216082/1‑101 (MQ=255)
                                                                               cACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCcgcg         >  2:4616718/1‑101 (MQ=255)
                                                                               cACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCcgcg         >  1:159487/1‑101 (MQ=255)
                                                                                aCCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCgcgc        >  1:881944/1‑101 (MQ=255)
                                                                                  cTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGccc      >  1:2527183/1‑101 (MQ=255)
                                                                                   ttGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGcccc     >  2:1834761/1‑101 (MQ=255)
                                                                                    tGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCa    >  1:1101066/1‑101 (MQ=255)
                                                                                    tGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCa    >  2:3224373/1‑101 (MQ=255)
                                                                                     gggAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGccccca   >  2:4241074/1‑99 (MQ=255)
                                                                                     gggAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAg   >  1:940491/1‑101 (MQ=255)
                                                                                              |                                                                                             
TTCTCTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AATAGTCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGC  >  NC_014034/3011194‑3011380

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG