Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,068,130 T→C 2.3% intergenic (‑113/+179) glcD ← / ← RCAP_rcc02872 glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,068,1300T→C 2.3% 3.1 ‑1.8 ‑0.3213intergenic (‑113/+179)glcD/RCAP_rcc02872glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (125/83):  total (130/83)
Fisher's exact test strand distribution p-value = 1.59e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.08e-01

Only 100 of 236 total aligned reads displayed.

CGCTCCGGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGC  >  NC_014034/3068030‑3068227
                                                                                                    |                                                                                                 
cgctccgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCt                                                                                                   <  2:2021235/101‑1 (MQ=255)
 gctccgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTc                                                                                                  >  1:4518581/1‑101 (MQ=255)
  ctccgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCg                                                                                                 <  1:1682845/101‑1 (MQ=255)
  ctccgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCg                                                                                                 >  1:1803448/1‑101 (MQ=255)
  ctccgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACAGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCg                                                                                                 >  1:4053753/1‑101 (MQ=255)
    ccgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTg                                                                                               <  1:1929401/101‑1 (MQ=255)
     cgGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGa                                                                                              >  2:345800/1‑101 (MQ=255)
      ggTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGCGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAc                                                                                             >  1:1159678/1‑101 (MQ=255)
      ggTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAc                                                                                             >  1:3059820/1‑101 (MQ=255)
      ggTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAc                                                                                             >  2:1425439/1‑101 (MQ=255)
       tctaaaaGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAcc                                                                                            <  2:589528/98‑1 (MQ=255)
       gTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAcc                                                                                            <  1:3000700/101‑1 (MQ=255)
        tCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTTAccg                                                                                           <  2:3741232/101‑1 (MQ=255)
        tCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCATCGTGAccg                                                                                           >  2:4203118/1‑101 (MQ=255)
         cAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAccgc                                                                                          >  2:1980615/1‑101 (MQ=255)
         cAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAccgc                                                                                          >  2:1285836/1‑101 (MQ=255)
         cAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAccgc                                                                                          >  1:3628125/1‑101 (MQ=255)
          aaaaGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAccgcc                                                                                         >  1:2994278/1‑101 (MQ=255)
           aaaGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGAccgccg                                                                                        >  2:2682876/1‑101 (MQ=255)
           aaaGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCAATCGCCGAGGACATGCGGAACCGTGGCCTCGTGAccgccg                                                                                        >  2:796883/1‑101 (MQ=255)
            aaGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGa                                                                                       >  2:1763574/1‑101 (MQ=255)
            aaGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGa                                                                                       <  2:4633245/101‑1 (MQ=255)
             aGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAg                                                                                      <  2:118012/101‑1 (MQ=255)
              gTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAgg                                                                                     >  2:4558573/1‑101 (MQ=255)
                tATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGc                                                                                   <  2:4501814/101‑1 (MQ=255)
                 aTGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGcc                                                                                  >  2:2204345/1‑101 (MQ=255)
                 aTGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGcc                                                                                  >  1:4177870/1‑101 (MQ=255)
                 aTGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGcc                                                                                  <  2:3313937/101‑1 (MQ=255)
                  tGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGccc                                                                                 >  1:278484/1‑101 (MQ=255)
                   gACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCa                                                                                <  2:1817204/101‑1 (MQ=255)
                    aCCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGcccga                                                                               <  1:3155092/101‑3 (MQ=255)
                      cAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAg                                                                             <  2:2528181/101‑1 (MQ=255)
                      cAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAg                                                                             >  2:3064462/1‑101 (MQ=255)
                       acttttGCGGATTCGCGCAACCGAACCGATGGGCAGGCTGACCGAGGACATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGCGCCCAAAgg                                                                            <  2:4707020/99‑1 (MQ=255)
                        aTTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGc                                                                           >  1:391359/1‑101 (MQ=255)
                           ttGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCt                                                                        <  2:4442325/101‑1 (MQ=255)
                            tGCGGATTCGCGCACCCGAAACGATGGGCAGGCTGACCGAGGCCACCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTc                                                                       <  1:2203934/101‑1 (MQ=255)
                            tGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTc                                                                       <  1:3621723/101‑1 (MQ=255)
                             gCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCg                                                                      <  2:2231455/101‑1 (MQ=255)
                             gCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCg                                                                      <  1:157151/101‑1 (MQ=255)
                               ggATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGc                                                                    <  2:3856044/101‑1 (MQ=255)
                                gATTCGCGCACCCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAAGAGTGGCCTCGTGACCGCCGAGGGCCCATAGGCCCTCGGcc                                                                   <  2:1565691/101‑1 (MQ=255)
                                gATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGcc                                                                   >  1:2124784/1‑101 (MQ=255)
                                gATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGcc                                                                   <  1:1841529/101‑1 (MQ=255)
                                 aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAACGGCCCTCGGCCa                                                                  <  1:2579267/101‑1 (MQ=255)
                                 aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCa                                                                  <  2:200985/101‑1 (MQ=255)
                                 aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCa                                                                  >  2:1115701/1‑101 (MQ=255)
                                 aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCa                                                                  <  1:938800/101‑1 (MQ=255)
                                 aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCa                                                                  >  2:956144/1‑101 (MQ=255)
                                    cgcgCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAgcg                                                               >  2:3551552/1‑101 (MQ=255)
                                    cgcgCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAgcg                                                               <  1:2459694/101‑1 (MQ=255)
                                       gcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAgcgcgc                                                            >  2:3504676/1‑99 (MQ=255)
                                        cAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGcgcccg                                                           <  1:1175181/101‑1 (MQ=255)
                                         aaCCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGCCCAGcgcccgc                                                          >  1:4386560/1‑101 (MQ=255)
                                           ccGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCgcccgccc                                                        <  2:28085/101‑1 (MQ=255)
                                            cGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGcccgcccc                                                       <  1:2681004/101‑1 (MQ=255)
                                             gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGcccgccccc                                                      >  2:2420912/1‑100 (MQ=255)
                                              aaaCGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGcccgccccgc                                                     >  1:3761338/1‑101 (MQ=255)
                                                aCGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCcgccccgccc                                                   <  1:325265/101‑1 (MQ=255)
                                                 cGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCa                                                  >  2:4413173/1‑101 (MQ=255)
                                                 cGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCa                                                  <  1:1108846/101‑1 (MQ=255)
                                                 cGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTAGGCCAGCGCCCGCCCCGCCCa                                                  <  1:4165361/101‑1 (MQ=255)
                                                  gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCac                                                 >  2:3244148/1‑100 (MQ=255)
                                                  gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCAt                                                 <  1:1401396/101‑1 (MQ=255)
                                                  gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCAt                                                 <  2:3005848/101‑1 (MQ=255)
                                                     gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCAAggc                                              >  1:2422370/1‑101 (MQ=255)
                                                      ggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcg                                             <  1:4558826/101‑1 (MQ=255)
                                                       gCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcgg                                            <  2:2590771/101‑1 (MQ=255)
                                                        cAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGGGCCCGCCCCGCCCACggcggg                                           >  1:2116629/1‑101 (MQ=255)
                                                            cTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCt                                       >  2:1381513/1‑101 (MQ=255)
                                                            cTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCt                                       <  2:200483/101‑1 (MQ=255)
                                                             tGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCtt                                      >  1:719015/1‑101 (MQ=255)
                                                                ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTtctc                                   <  1:4665731/101‑1 (MQ=255)
                                                                 cGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcg                                  >  1:3503987/1‑101 (MQ=255)
                                                                 cGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcg                                  <  2:4398398/101‑1 (MQ=255)
                                                                  gAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCCTGGCGGGCGCTTCTcgc                                 >  2:1689756/1‑101 (MQ=255)
                                                                   aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgcc                                <  1:3616007/101‑1 (MQ=255)
                                                                    ggCCAGCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgccc                               <  2:3264755/101‑1 (MQ=255)
                                                                        aTCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCccgccg                           <  1:3686174/101‑1 (MQ=255)
                                                                         tCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCgg                          <  1:1115787/101‑1 (MQ=255)
                                                                           gCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCgggg                        <  1:2815010/101‑1 (MQ=255)
                                                                           gCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCgggg                        <  1:1285927/101‑1 (MQ=255)
                                                                            ccGAGGTCATGCGGACCAGTGGCCTCGTGACCGCCGCGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGgggc                       <  1:2260916/101‑1 (MQ=255)
                                                                             cGAGGTCATGCGGAACAGTGGCCTCGTGACGGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCCCCGgggcg                      >  1:4223035/1‑101 (MQ=255)
                                                                             cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGgggcg                      >  1:477412/1‑101 (MQ=255)
                                                                             cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGgggcg                      <  1:3844814/101‑1 (MQ=255)
                                                                             cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCCCCcggggg                      >  1:1733475/1‑99 (MQ=255)
                                                                              gAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCCgggcgg                     >  2:648483/1‑101 (MQ=255)
                                                                               aGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGgggcggg                    >  1:2000735/1‑101 (MQ=255)
                                                                               aGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCCCCCggggggg                    >  2:4284063/1‑101 (MQ=255)
                                                                                 gTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGgggcggggg                  >  1:4462986/1‑99 (MQ=255)
                                                                                    aggCGGAACAGTGGCCGCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCGGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCg               <  1:647431/99‑1 (MQ=255)
                                                                                     tGCGGAACAGTGGCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCgg              <  1:1019091/101‑1 (MQ=255)
                                                                                           aCAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCccctc        >  2:3639427/1‑100 (MQ=255)
                                                                                              gTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGGGGCGGcccccgccg     >  1:878104/1‑94 (MQ=14)
                                                                                               tGGCCTGGGGCCCGCGGGGGGCCCAAGGGCCCTCGCCCAGCGCCCGCCCCGCCCAGGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCtgctgc    <  2:1896427/101‑1 (MQ=11)
                                                                                               tGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCCGCCCctgctcc    >  2:2444994/1‑99 (MQ=255)
                                                                                               gggCCCCGTGCCCGCCGAGGGCCCAAGGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCtgctgc    <  1:1425533/100‑1 (MQ=255)
                                                                                                ggCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCCGCCCCCGGGGCGGGGGGCGGCCCCCGCCgcg   >  1:962572/1‑101 (MQ=14)
                                                                                                 gCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTctgcccgat  >  2:2196913/1‑96 (MQ=255)
                                                                                                    |                                                                                                 
CGCTCCGGTCAAAAGTTATGACCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGC  >  NC_014034/3068030‑3068227

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG