BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,068,179	A→T	2.5%	intergenic (‑162/+130)	glcD ← / ← RCAP_rcc02872	glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,068,179	0	A→T	2.5%	3.0 ‑2.0 ‑0.3	159	intergenic (‑162/+130)	glcD/RCAP_rcc02872	glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand): new base (4/0): ref base (88/67): total (92/67)
Fisher's exact test strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.59e-01

Only 100 of 227 total aligned reads displayed.


CGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAG  >  NC_014034/3068079‑3068279


cGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCa                                                                                                           <  1:1108846/101‑1 (MQ=255)


cGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCa                                                                                                           >  2:4413173/1‑101 (MQ=255)


cGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTA.GGCCAGCGCCCGCCCCGCCCa                                                                                                           <  1:4165361/101‑1 (MQ=255)


 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCac                                                                                                          >  2:3244148/1‑100 (MQ=255)


 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCAt                                                                                                          <  2:3059632/101‑1 (MQ=255)


 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCAt                                                                                                          <  1:1401396/101‑1 (MQ=255)


  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCacg                                                                                                         >  1:3896909/1‑99 (MQ=255)


  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCacg                                                                                                         >  2:4442714/1‑99 (MQ=255)


  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATg                                                                                                         <  2:2452543/101‑1 (MQ=255)


   tGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GACCAGCCGCCGCCCCGCCCATgg                                                                                                        >  2:803277/1‑101 (MQ=255)


    gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATggc                                                                                                       >  1:1600173/1‑101 (MQ=255)


    gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATggc                                                                                                       <  2:4151196/101‑1 (MQ=255)


      gCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATggc.gg                                                                                                    <  2:2590771/101‑1 (MQ=255)


       cAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGGGCCCGCCCCGCCCACggc.ggg                                                                                                   >  1:2116629/1‑101 (MQ=255)


           cTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.t                                                                                            <  2:200483/101‑1 (MQ=255)


            tGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.tt                                                                                           >  2:183647/1‑101 (MQ=255)


            tGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.tt                                                                                           <  1:4685412/101‑1 (MQ=255)


            tGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.tt                                                                                           >  1:719015/1‑101 (MQ=255)


            cgcccGAGGCGATCGCCGAGGTGATGCGGAACAGTGGCTTCGGGCCCGCCGAGGGCCCAAA.GG.CCCCC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.tt                                                                                           <  2:129154/98‑1 (MQ=17)


               ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.Ttctc                                                                                        <  1:2428552/101‑1 (MQ=255)


                cGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTcg                                                                                       >  1:3503987/1‑101 (MQ=255)


                cGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTcg                                                                                       <  2:4398398/101‑1 (MQ=255)


                cGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCATCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTcg                                                                                       <  1:4203364/101‑1 (MQ=255)


                  aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTcgcc                                                                                     <  1:3616007/101‑1 (MQ=255)


                  aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTcgcc                                                                                     >  2:2202048/1‑101 (MQ=255)


                  aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTcgcc                                                                                     <  1:2924713/101‑1 (MQ=255)


                  aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCCCCCATGGC.GGGC.G.C.tttccccc                                                                                     >  1:1826205/1‑95 (MQ=255)


                      cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTcgcc.cgcc                                                                                <  2:1686385/101‑1 (MQ=255)


                      cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCCCCCATGGC.GGGC.G.C.TTCTcccc.cccc                                                                                >  1:2663382/1‑101 (MQ=255)


                       aTCGCCGAGGTCTTCCGTAAAAGTGGCCTCGGGACCGCCGAGGGCCCAAA.GC.CCCCC.GCCCAGGGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCc.cgccg                                                                               <  2:1159592/101‑1 (MQ=17)


                         cGCCGAGGTCATGCGGACCAGAAGTCCCGTGACCGCCGGGGGCCCAAA.GG.CCCTC.GGCCTGCGCCCGCCCCGCCATCGGC.TGGC.G.C.GGCTCCCCGCGCCgg                                                                              >  2:1769600/1‑101 (MQ=18)


                           ccGAGGTCATGCGGACCAGTGGCCTCGTGACCGCCGCGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGgggc                                                                           <  1:2260916/101‑1 (MQ=255)


                           ccGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGgggc                                                                           >  2:266490/1‑101 (MQ=255)


                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGG.G.C.CTCTCGCC.CCCCGgggcg                                                                          >  1:1338927/1‑101 (MQ=255)


                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGgggcg                                                                          >  2:2715033/1‑101 (MQ=255)


                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGgggcg                                                                          <  1:3844814/101‑1 (MQ=255)


                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.CTCTCGCC.CCCCCgggcg                                                                          >  2:1892998/1‑101 (MQ=255)


                            cGAGGCCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGgggcg                                                                          <  2:454454/101‑1 (MQ=255)


                              aGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGgggcggg                                                                        >  1:2000735/1‑101 (MQ=255)


                                  cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGGGcc                                                                    >  1:3151551/1‑101 (MQ=255)


                                    tGCGGAACAGTGGCCTCGTGCCCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCgg                                                                  <  1:1019091/101‑1 (MQ=255)


                                    tGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCgg                                                                  >  1:3756844/1‑101 (MQ=255)


                                         accggTGCCCTCGTGCCCGCCGAGGCCCAAAA.GC.CCCTC.GGCCAGCGCCCGCCCCGCCCAGGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCctct                                                             <  1:1763682/97‑1 (MQ=14)


                                           cgggggCCTCGTGCCCCCCGAGGCCCAAAA.GG.CCCTC.GGCCACCGCCCGCCCCGCCCATGGG.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTctgc                                                           <  1:1160519/97‑1 (MQ=14)


                                           caatGGCCCCGTGACCGGCGAGGGCCCAAA.GG.CCCTC.GGCCTGCGCCCGCCCCGCCATCGGC.GGGC.G.CGGTCGCGCC.GCCCGGGGGGGGGGGCgggccccg                                                            >  2:1955833/4‑95 (MQ=17)


                                           cAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCCctgc                                                           >  2:2847374/1‑101 (MQ=255)


                                             gTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGGGGCGGcccccgccg                                                         >  1:878104/1‑94 (MQ=14)


                                             gTGGCCTCGCGACCGCCGA.GGCCCAAA.GGCCCCTC.GCCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTctgctg                                                         <  1:1986869/101‑1 (MQ=14)


                                              tGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CCCCCGGGAGGGGGCCAGCCTCtgctgc                                                        >  1:3644631/1‑101 (MQ=17)


                                               ggCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCCGC.CCCCGGGGCGGGGGGCGGCCCCCGCCgcg                                                       >  1:962572/1‑101 (MQ=14)


                                               ggCCCCGTGACCGGCGAGGGCCCAAA.GG.CCCTC.GGCCTGCGCCCGCCCCGCCATCGGC.GGGCGG.C.GTCGCGCC.CCCCGGGGCGGGGGCGgggccgccggtg                                                        >  1:919207/1‑91 (MQ=14)


                                                 ccccgggcccgccgAGGGCCCAAA.GC.CCCTC.GGCCAGCCCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGcc                                                     <  1:1381607/93‑1 (MQ=14)


                                                  ctggTGCCCGCCGA.GGCCCAAA.GGCCCCTG.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCt                                                    <  2:2124663/98‑1 (MQ=14)


                                                  cccgggcccgccgGGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCAGGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCt                                                    <  1:3565571/94‑1 (MQ=11)


                                                    ggtcaCCGCCGAGGGCCCAAA.GC.CCCTC.GGCCCGCGCCCGCCCCCCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTgg                                                  <  2:757866/97‑1 (MQ=14)


                                                    cGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCCgg                                                  >  1:4427523/1‑101 (MQ=255)


                                                     gggcccgccgAGGCCCCAAG.GC.CCCTC.GGCCAGCCCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTggg                                                 <  1:333918/97‑1 (MQ=14)


                                                     gTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TCTTCGCC.CGCCCGGGCGGGCGGCGGCCGCCTCTCCGCCTggg                                                 >  1:2398583/1‑101 (MQ=14)


                                                      tGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCCGCCCCTGCCGCCCCCgggg                                                >  1:4639127/1‑101 (MQ=14)


                                                       gACCGGCGAGGGCCCAAA.GG.CCCTC.GGCCTGCGCCCGCCCCGCCATCGGCGGGGG.G.G.GGCGCGCC.CCCCGGGGGGGGGGCCGGGCCCCGGTGGGGCTgggg                                                >  1:2817410/1‑101 (MQ=2)


                                                       gACCGCCGAGGGCCCAAA.GG.CACTC.GGCCTGCGCCCGCCCCGCCATCGGC.GGGG.GCC.CGCGCGCC.CCCCGGGGGGGGGGGCGGCCCccgcttctgtttggg                                                >  2:106655/1‑91 (MQ=2)


                                                         ccgcggGGGGCCGAAG.GC.CCCCC.GCCCGGGGCCCCCCCCGCGCCTGGC.GGGC.G.C.TTCCCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGaaa                                             <  1:499123/101‑1 (MQ=9)


                                                          cgcggAGGGCCCAAG.GG.CCCGC.GGCCGGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAAc                                            <  2:4193045/101‑1 (MQ=17)


                                                            ccgAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCCCTGCTGCGCCCGGGGGAACgg                                          >  2:80737/1‑101 (MQ=255)


                                                            cccggggCCCAAACGC.CCCGC.GGCCGGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACg                                           <  2:1959938/97‑1 (MQ=14)


                                                             cgggggCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGt                                         <  1:1719466/98‑1 (MQ=255)


                                                             cgAGGGCCCAAG.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGt                                         <  1:1944472/101‑1 (MQ=255)


                                                             ccggggCCCAAA.GG.CCCCC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGG.G.C.TTCTCCCC.CCCCGGGGCGGGGGCCGGCCCCTGTCACGCCTtggggcaccgg                                         >  1:1033255/4‑94 (MQ=14)


                                                              gggggCCCAAA.GG.CCGCC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGG.G.G.GTCTCGCC.CCCCCGGCCCGGCGGCGGGCCCGGGGGCGCCTcgggggcggggc                                        >  2:2485955/3‑93 (MQ=2)


                                                              gAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCCCTGCTGCGCCTGGGGAAACggcc                                        >  2:1084985/1‑99 (MQ=255)


                                                                ggccccACA.GC.CCCCC.TGCCGGCGCCCCCCCCGCCCAGGGC.GGGC.C.C.TTCTGGCC.CGCCGGGGGGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCtt                                      <  2:2422235/98‑1 (MQ=17)


                                                                  gCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCCGGGGAAACGGTCTTgc                                    >  2:1357513/1‑100 (MQ=255)


                                                                   cccCAA.GG.CCCCCGGGCCGGCGCCCCCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTgg                                    <  2:3210680/101‑1 (MQ=17)


                                                                   cccAAG.GG.CCCCC.GGCCAGCGCCCCCCCCGCCCGTGGC.GGGC.G.C.TTCTCGCC.CGCGGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGc                                   <  1:1889427/101‑1 (MQ=17)


                                                                    ccAAAGGG.CCCTC.GGCCGGCGCCCCCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGc                                   <  2:2250319/101‑1 (MQ=18)


                                                                     cAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGGGGCGGCCCCTGCCGCGCCCGGGGGAACGGGCTTGGCAc                                 >  2:954342/1‑101 (MQ=17)


                                                                      aaa.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGGGCCGGCCTCTGCCGCGCCCGGGGCAACGGTCTTGGCAcc                                >  1:1253773/1‑101 (MQ=17)


                                                                      aaa.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCCCTGCTGCGCCTGGGGGAACGGGCTTGGCAcc                                >  2:3376295/1‑101 (MQ=255)


                                                                       aaggg.CCCTG.GGCCGGCCCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGGGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCAcc                                <  2:3627906/98‑1 (MQ=18)


                                                                       aa.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCCGCCTCTTCTGCGCCCGGGGAAACGGTCTTgggcccg                               >  2:3954947/1‑96 (MQ=17)


                                                                        a.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGGGCCCGCCTCTGCCGCGGCTGGGGGAACGGTCTTGGCACCGc                              >  2:568190/1‑101 (MQ=17)


                                                                         aggccccGC.GCCCAGCCCCCGCCCCGCCCAGGGG.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCg                               <  2:2107152/97‑1 (MQ=18)


                                                                          gg.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGGAAAGGTCTTGGGACCGcc                             >  2:1627867/1‑101 (MQ=255)


                                                                          gg.CCCGC.GCCCACCGCCCCCCCCCCCNNGGNC.GGGC.C.C.TTCGCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGcc                             <  1:3244349/101‑1 (MQ=18)


                                                                           g.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCGGCTGCGCCTGGGGAAACGGGCTTGGCACCGccc                            >  1:4053501/1‑101 (MQ=255)


                                                                             cccGC.GCCCAGCCCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCg                           <  1:4473553/101‑1 (MQ=255)


                                                                                   ggCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGGCGGCCTCTGCCGCGGCTGGGGGAAGGGGCTTAGCACCGCCCCGGCTc                      >  1:2794653/1‑101 (MQ=17)


                                                                                      cacccccccccccccCCATGGG.GGGC.C.C.TTCTCGCC.CGCCGGGGCGGGCCCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCcgg                   <  2:3543515/96‑1 (MQ=21)


                                                                                         cgcccgccCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCcggcgg                >  2:1809897/1‑101 (MQ=255)


                                                                                             cgccccgcccAGGGC.GGGC.G.C.TTCCCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGgcgggcgg            <  1:2342447/101‑1 (MQ=255)


                                                                                             ccccccgcccAGGGC.GGGC.G.C.TTCCCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGgcgggcgg            <  1:4284310/99‑1 (MQ=255)


                                                                                                cccgcccAGGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGa         <  2:4658082/101‑1 (MQ=255)


                                                                                                 ccgcccCGGGC.GGGC.C.C.TTCCCGCC.CGCGGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGaa        <  2:3760299/101‑1 (MQ=21)


                                                                                                 ccgcccAGGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGaa        <  1:4574123/101‑1 (MQ=255)


                                                                                                    cccATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAgg     >  2:1569021/1‑101 (MQ=255)


                                                                                                    cccATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAgg     >  2:67593/1‑101 (MQ=255)


                                                                                                     ccAGGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGGGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAACGc    <  1:2712650/101‑1 (MQ=255)


                                                                                                       aTGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGGGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGGAAGGCAg  >  1:3625017/1‑101 (MQ=255)


                                                                                                       aTGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGGAAGGCAg  >  1:4543351/1‑101 (MQ=255)


                                                                                                       aTGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCCGGGGAAACGGTCTTTGCACCCCCCCGGCTCCGGCGGGCGGGGAAAGGCAg  >  2:3640883/1‑101 (MQ=21)


CGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGC.G.C.TTCTCGCC.CGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGCGGGCGGGGAAAGGCAG  >  NC_014034/3068079‑3068279

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 22 ≤


ATCG

< 32 ≤


ATCG

< 40 ≤


ATCG