Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,137,302 C→A 7.8% L104F (TTG→TTT)  RCAP_rcc02947 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,137,3020C→A 7.8% 6.2 ‑1.7 ‑0.075L104F (TTG→TTT) RCAP_rcc02947hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/6):  ref base (21/48):  total (21/54)
Fisher's exact test strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.91e-01

Only 100 of 186 total aligned reads displayed.

TGCTCGCATATGGCGCCTCGCGCGACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTTCTCTGACCGACCGATCAAGGCTCA  >  NC_014034/3137202‑3137402
                                                                                                       |                                                                                                     
tGCTCGCATATGGCGCCTCGCGCGACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTTTGGCCC.T..CCCCCGGCCGc                                                                                                       >  1:2851863/1‑101 (MQ=255)
 gCTCGCATATGGCGCCTCGCGCGACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.a                                                                                                     <  2:3840609/101‑1 (MQ=255)
 gCTCGCATATGGCGCCTCGCGCGACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCCAGGCCGC.a                                                                                                     >  2:135584/1‑101 (MQ=255)
  cTCGCATATGGCGCCTCGCGCGACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.aa                                                                                                    <  2:1425540/101‑1 (MQ=255)
    cGCATATGGCGCCTCGCGCGACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTTTGGGCC.T..CCCCGGGCCGC.CAGc                                                                                                  >  2:3777616/1‑101 (MQ=255)
    cGCATATGGCGCCTCGCGCGACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTTCGGGCC.T..CCCCCGGCCGC.ACGc                                                                                                  >  1:3109985/1‑101 (MQ=255)
     gCATATGGCGCCTCGCGCGACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTTCGGCCC.T..CCCCCGGCCGcccgcc                                                                                                  >  1:161502/1‑96 (MQ=255)
     gCATATGGCGCCTCGCGCGACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGcc                                                                                                 <  1:1124568/101‑1 (MQ=255)
     gCATATGGCGCCTCGCGCGACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGcc                                                                                                 <  1:1732422/101‑1 (MQ=255)
      cATATGGCGCCTCGGGCGACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGGGGCCGC.AAGCCg                                                                                                <  2:1587992/101‑1 (MQ=255)
         atGGCGCCTCGCGCGACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCCGCGGCCA.T..CCCGAGGCCGC.AAGCCCAgg                                                                                             >  2:675185/1‑101 (MQ=255)
          tGGCGCCTCGCGCGACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAggg                                                                                            >  1:3070852/1‑100 (MQ=255)
           ggCGCCTCGCGCGACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTg                                                                                           <  1:3653417/101‑1 (MQ=255)
           ggCGCCTCGCGCGACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTg                                                                                           <  2:2564065/101‑1 (MQ=255)
             caccTCGCGCGACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGCGGCCGC.AAGCCGAGGTGaa                                                                                         <  1:2321376/99‑1 (MQ=255)
                 ccgcgcgACAGAGCGAGAACGGGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTc                                                                                     <  2:2602471/100‑1 (MQ=255)
                  cgcgcgACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTcc                                                                                    <  1:3054509/101‑1 (MQ=255)
                   gcgcgACAGAGCGAGAACGGGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCa                                                                                   <  1:3607440/101‑1 (MQ=255)
                    cgctgcAGAGAGAGAACGGGGGCCTTCCGCTCGGGGATTTTTGCCGGGAATGCCAGGGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCaa                                                                                  <  2:1734842/96‑1 (MQ=255)
                    cgcgACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCaa                                                                                  <  1:3788812/101‑1 (MQ=255)
                       gACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCGTGCGGCCA.T..CCCCAGGCCGC.CAGCCGCGGGGGAAGGGCAAGaa                                                                               >  2:2010841/1‑101 (MQ=255)
                       gACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTTGGGGCA.C..CCCCCGGCCGC.AAGCCCAGGGGAAAAGCCAAAaa                                                                               >  1:3706503/1‑101 (MQ=255)
                       gACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGGCA.T..CCCGCGGCCGC.AAGCCGAGGGGAAAGGCCAAAaa                                                                               >  2:2344055/1‑101 (MQ=255)
                        aCAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCC.T..CCCCAGGCCGC.CAACCGCGGGGGAAAACCAAAaaa                                                                              >  1:2737317/1‑101 (MQ=255)
                         cAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATCCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAAt                                                                             <  2:4652554/101‑1 (MQ=255)
                         cAGAGCGAGAACGAGGGCGTTCCGCTCGGAGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAAt                                                                             <  2:2783340/101‑1 (MQ=255)
                           gagCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTTCGGGCC.C..CCCCCGGCCGC.CAGCCGCGGGGAAAAGCCCAGAAAACg                                                                           >  2:1812987/1‑101 (MQ=255)
                            agCGAGAACGGGGGCGTTCCGCTCGGCGATTTTTGCCGTAAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGt                                                                          <  2:3458541/101‑1 (MQ=255)
                            agCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTTCGGCCC.T..CCCCCGGGCGC.AAGCCGAGGGGAAAAACCAAAaaaaaag                                                                          >  1:1613653/1‑97 (MQ=255)
                               gagaACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTTCGGCCA.T..CCCCCGGCCGC.CAGCCGCGGGGGANGTCCAAGAAATACTGCt                                                                       >  2:91274/1‑101 (MQ=255)
                               gagaACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCCGGGCCGC.AAGCCGAGGGGAAAAGCCAAAAAAAAGTGCt                                                                       >  1:589492/1‑101 (MQ=255)
                                agaACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGGCAGC..CCCGAGG.CGC.CAACCGAGGGGGAAGTACAAAaaaaaatgcgg                                                                      >  2:1114954/1‑93 (MQ=255)
                                  aaCGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTTCGGGCA.T..TCCCCGGCCCC.AAGCCGAGGGGAAAGACCAACAAAAAGTGCTGGc                                                                    >  1:2654222/1‑101 (MQ=255)
                                  aaCGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTTCGGGCA.T..CCCCAGGCCGC.AAGCCGAGGGGAAAGACCCAGAAAACGTGCTGGc                                                                    >  2:2090190/1‑101 (MQ=255)
                                   aCGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGcc                                                                   <  2:3070664/101‑1 (MQ=255)
                                    cGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTTCGGCCC.T..CCCCAGGCCGC.AAGCCGAGGGGAAAGGCCAAAAAAAAGTGCTGGCCg                                                                  >  2:3677604/1‑101 (MQ=255)
                                    cGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCC.T..CCCGAGGCCGC.AAGCCGAGGGGGAAGGCCAAGAAATCGTGGTGGGCg                                                                  >  2:4557258/1‑101 (MQ=255)
                                    cGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCC.T..CCCCCGGGCGC.AAGCCCAGGGGGAAGTCCCAGAAAAAGTGCTGGCCg                                                                  >  2:1729458/1‑101 (MQ=255)
                                     gAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCC.T..CCCCAGGGCGC.CAGCCGCGGGGGAAGACCAAGAAAACGTGGTGGCCgg                                                                 >  1:3977170/1‑100 (MQ=255)
                                       gggCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTTCGGCCA.T..CCCCCGGCCGC.CAGCCTGGGGGGAAGGCCAAGAAAAAGTGCTGGGCgagg                                                               >  1:4107980/1‑100 (MQ=255)
                                        ggtgTTCCGCTGGGCGTTTTTTGCGGTGAATGCCAGCGGCCTGGGGCCA.T..CCCGGGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGAt                                                              <  1:170937/98‑1 (MQ=255)
                                         gCGTTCCGCTGGGCGATTTTTGCCGTGAATCCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATc                                                             <  2:2900054/101‑1 (MQ=255)
                                          ggTTCCGCTCGGCGTTTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCg                                                            <  1:4296880/100‑1 (MQ=255)
                                            ttCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTTCGGCCA.T..CCCCAGGCCGC.CAGCCGAGGGGAAAAGCCAAGAAAAAGTGGTGGCCgggaaagct                                                          >  1:116411/1‑96 (MQ=255)
                                             tCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCtt                                                         <  2:2170451/101‑1 (MQ=255)
                                              ccGCTCGGCGTTTTTTGCCGTGAATGCCGGGGCCCTGCGGCCA.C..CCCGGGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCttt                                                        <  1:3496233/101‑1 (MQ=255)
                                              ccGCTCGGCGTTTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCttt                                                        <  1:3403486/101‑1 (MQ=255)
                                              ccGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTTCGGCCC.T..CCCCGGGCCGC.CAGCCCGGGGGGAAGGCCCAGAAAACGTGCTGGCCgaagacgcctt                                                        >  2:1631734/1‑92 (MQ=255)
                                              ccGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGGCC.TGCCCCGGGGCCG..AGCCCCGGGGGAAAG.CCAAGAAAACGAGCTGGCCgagagtacctt                                                        >  1:2178687/1‑94 (MQ=255)
                                              ccGCTCGGAGATTTTTGCTGTGAAGCCTAGCGGCCGGGGACCC.T..CCCGAGCCAGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCttt                                                        <  1:2754873/101‑1 (MQ=255)
                                               cGCGCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTc                                                       <  2:3928018/101‑1 (MQ=255)
                                                gCTCGGCGTTTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCg                                                      <  2:3197087/101‑1 (MQ=255)
                                                 cTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTTCGGCCA.T..CCCCAGGCCGC.CAGCCCCGGGGAAAGTCCAAGAAAACGTGCGTGCCGAGATAGCTTTcca                                                     >  2:1164874/1‑99 (MQ=255)
                                                  tgggCTATTTTGGCCGTGAATGCCAGCGGCCGGGGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAg                                                    <  2:628916/99‑1 (MQ=255)
                                                  tCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCCCGGCCGC.AAGCCGAGGGGGAAATACAAAAAAACGTGCTGGCCGAGAACGCttttggc                                                    >  1:206447/1‑97 (MQ=255)
                                                   cGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCC.T..CCCGAGGCCGC.CAGCCGGGGGGAAAAACCAAGAAAAAGTGCCTGGCGGGGTCGCTTTTGAGc                                                   >  1:2979590/1‑101 (MQ=255)
                                                   cGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.C..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGc                                                   <  1:4178998/101‑1 (MQ=255)
                                                     gctttttttGCCGTGAAGCCCAGCGGCCGGCGGCCA.C..CCCGAGGCCGA.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCa                                                 <  2:1476716/97‑1 (MQ=255)
                                                     gctttttttGCCGAAAATGCCAGCGGCCTGCGGCAA.C..CCCGAGGCCAA.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCa                                                 <  1:4500911/97‑1 (MQ=255)
                                                     gCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCC.T..CCCCAGGCCGC.AAACCGCGGGGGAAATCCAAGAAAACGTGCTTGGCGAGAGCGCTTTCGAGCCa                                                 >  2:1603157/1‑101 (MQ=255)
                                                      cgttttttGCCGTGAGGCCCGGCGGCCGGGGGCCA.T..CCCGAGGCCGA.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCaa                                                <  2:1044723/98‑1 (MQ=255)
                                                      cGATTTTTGCCGTGAATGCCAGCGGCCTTGGGCCG.T..CCCCAGGGGGC.AAGCCGAGGGGGAAAGCCCAGAAAACGGGCTGGGCGGGATCCCTTTCGTGCCaa                                                >  1:1872398/1‑101 (MQ=255)
                                                      cGATTTTTGCCGTGAATGCCAGCGGCCTGGGGTCA.T..TCCAAAGACGC.AAAACGCGGGGAAAGGCCAAAAAAAATTGCTGGGCGGGAGCGCctttcaacccc                                                >  1:713914/1‑93 (MQ=255)
                                                        ttttttGCCGTGAATGCCGGCGGCCTGCGCCCA.C..CCCGGGCCCGA.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATc                                              <  1:3235314/100‑1 (MQ=255)
                                                        aTTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATc                                              <  1:3950094/101‑1 (MQ=255)
                                                         tttttGCCGTGAATGCCAGCGGCCTGCGGGCC.T..CCCGAGGCCGC.AAGCCGAGGGGGAAGGCCAAGAAATAGTGCTGGCCGAGATACGTTTCGAGCCAAtag                                             >  2:724206/1‑99 (MQ=255)
                                                          ttttGCCGTGAATGCCAGCGTCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATcgc                                            >  1:3160090/1‑101 (MQ=255)
                                                          ttttGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCCAGGCCGC.AAGCCGAGGGGAAAGTCCAAGAAAACGTGCTGGCCGAGATCGCTTTTGCGCCAATcgc                                            >  1:2404514/1‑101 (MQ=255)
                                                          ttttGCCGTGAATGCCAGCGGCCTGAGGCCA.C..CCCGAGGGCGC.CAGGCCAGGTGAAAGTACCAAAAAACGTGCTGGCCGGGGTCGCCTTTCAGCCAATccc                                            >  2:4514649/1‑99 (MQ=255)
                                                          ttttGCCGGAAAGCCCGGCGGCCGGGGGCCC.C..CCCGGGGCCGA.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATcgc                                            <  2:3354411/101‑1 (MQ=255)
                                                             tGCCGTGAATGCCAGCGGCCTGCGGCCC.T..CCCCCGGCCGC.CAGCCGAGGGGAAAGTCCAAGAAATCGTGCTGGCCGAGATGGCTTTCGAGGCAATCGCggg                                         >  2:4752149/1‑100 (MQ=255)
                                                               ccggaaaTGCCGGGGGCCGGCGCCCA.C..CCGGGGCCCGA.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATc                                       <  1:2402594/96‑1 (MQ=255)
                                                               ccGTGAATGCCAGCGGCCTGCGGGCA.T..CCCGAAGCCGC.CAGCCGGGGGGGAAGGCCCAGAAATCGTGCTGGCCGAGACCGCTTTCGGGCCAACCGCGGaac                                       >  2:737767/1‑99 (MQ=255)
                                                                cGTGAATGCCAGCGGCCTGCGGCCA.T..CCCCAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAAACGTGCTGGCCGCGATCGCTTTCGAGCCAAACGCGGGTCa                                      >  2:4402110/1‑101 (MQ=255)
                                                                cGTGAATGCCAGCGGCCTGCGCCCA.C..CCCGAGGCCGA.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCa                                      <  2:3055980/101‑1 (MQ=255)
                                                                 ggaaagcccggCGGCCGGCGGCCA.C..CCGGAGGCCGA.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCaa                                     <  1:2040267/91‑1 (MQ=255)
                                                                  taaaTGCCGGCGGCCTGCGCCCA.C..CCGGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCaaa                                    <  1:4438370/99‑1 (MQ=255)
                                                                    aaTGCCAGCGGCCTGCGGGCA.T..CCCGAGGCCGC.AAGACGCGGGGGAAGACCATAAAAAAGGGCTGGCCGAGATCGCGTTAGGGCCAGACGCGGagccaaac                                  >  2:3170341/1‑94 (MQ=255)
                                                                     tgccgtgCGGCCCGCGCCCC.C..CCCGAGGCCTC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGcc                                 <  1:293653/95‑1 (MQ=255)
                                                                     aTGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGGGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGGTCAAAGcc                                 >  1:3735544/1‑101 (MQ=255)
                                                                      tGCCAGCGGCCTGCGGCCA.T..CCCCAGGCCGC.AAACCGCGGGGAAAGGCCAAGAAAACGTGCTGGCCGCGATCGCTTTCGGGCCAATCGCGGGTCCAAACCg                                >  2:3256280/1‑101 (MQ=255)
                                                                            cgcccggCGCCCA.C..CCCGGGCCCGA.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGtt                          <  2:2984169/95‑1 (MQ=255)
                                                                             ggCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTtc                         >  2:3149376/1‑101 (MQ=255)
                                                                             ggCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTtc                         >  2:544414/1‑101 (MQ=255)
                                                                             ggCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTtc                         >  1:192310/1‑101 (MQ=255)
                                                                                cTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTTctct                      >  2:698892/1‑101 (MQ=255)
                                                                                  gCGCCCA.C..CCGGAGGCGAA.AAGCGGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTTCTCTga                    <  1:1337281/101‑1 (MQ=255)
                                                                                   cGGCCA.T..CCGGCGGCCAA.AAGCCGAGGTGAAAGTCCAAGAAATCGGGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTTCTCTgac                   <  1:3003863/101‑1 (MQ=255)
                                                                                     tgcggccccccGGGGCCGGAAAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTTCTCTg                     <  2:4578682/93‑1 (MQ=255)
                                                                                     gCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTTCTCTgaccg                 >  2:1191754/1‑101 (MQ=255)
                                                                                          t..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTTCTCTGaccgaccg             >  2:2841784/1‑101 (MQ=255)
                                                                                            cccggAGGCCGA.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTTCTCTGaccgaccg             <  2:3109785/97‑1 (MQ=255)
                                                                                             cccGAGGCCGA.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTTCTCTGAccgaccga            <  2:4087162/101‑1 (MQ=255)
                                                                                              cggagccgac.AATCCGGGGTAAAAGTCAAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTTCTCTGACCGACCGAt           <  1:802453/92‑1 (MQ=255)
                                                                                               gggggCCGA.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTTCTCTGACCGACCGATc          <  1:4752405/98‑1 (MQ=255)
                                                                                               cGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTTCTCTGACCGACCGATc          >  2:1380013/1‑101 (MQ=255)
                                                                                                gggcccGA.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTTCTCTGACCGACCGATCa         <  2:3358316/97‑1 (MQ=255)
                                                                                                     cGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTTCTCTGACCGACCGATCAAGGCt    >  2:4253289/1‑101 (MQ=255)
                                                                                                      gC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTTCTCTGACCGACCGATCAAGGCTc   >  2:1922565/1‑101 (MQ=255)
                                                                                                       c.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTTCTCTGACCGACCGATCAAGGCTCa  >  1:2015901/1‑101 (MQ=255)
                                                                                                       |                                                                                                     
TGCTCGCATATGGCGCCTCGCGCGACAGAGCGAGAACGAGGGCGTTCCGCTCGGCGATTTTTGCCGTGAATGCCAGCGGCCTGCGGCCA.T..CCCGAGGCCGC.AAGCCGAGGTGAAAGTCCAAGAAATCGTGCTGGCCGAGATCGCTTTCGAGCCAATCGCGGATCAAAGCCGACAGTTCTCTGACCGACCGATCAAGGCTCA  >  NC_014034/3137202‑3137402

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG