Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 317,927 A→G 100% K338E (AAG→GAG)  RCAP_rcc00280 → hemolysin‑type calcium‑binding repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034317,9270A→G100.0% 954.5 251K338E (AAG→GAG) RCAP_rcc00280hemolysin‑type calcium‑binding repeat family protein
Reads supporting (aligned to +/- strand):  new base (123/127):  ref base (0/0):  total (123/128)

Only 100 of 255 total aligned reads displayed.

AGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCAAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATCCGC  >  NC_014034/317830‑318024
                                                                                                 |                                                                                                 
aGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAgg                                                                                                >  2:1060286/1‑101 (MQ=255)
 gTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGa                                                                                               <  1:1044997/101‑1 (MQ=255)
   gACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGAtg                                                                                             <  2:595544/101‑1 (MQ=255)
      gCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGc                                                                                          <  2:3622909/101‑1 (MQ=255)
       cTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGct                                                                                         >  2:3728930/1‑101 (MQ=255)
       cTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGct                                                                                         <  2:2264305/101‑1 (MQ=255)
        tGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGctc                                                                                        >  2:9189/1‑101 (MQ=255)
          gAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCtt                                                                                      >  1:4187912/1‑101 (MQ=255)
           aGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTg                                                                                     <  2:1754086/101‑1 (MQ=255)
            gCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGCGGTCGATGCGGGCGATCTGCGCCCCGAGGAtgtgagatcgg                                                                                    >  2:3305988/1‑94 (MQ=255)
              ccGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATc                                                                                  <  2:3090991/101‑1 (MQ=255)
               cgcgACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCa                                                                                 <  2:1591662/101‑1 (MQ=255)
                 cgacgaCCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACg                                                                               <  2:4187666/101‑1 (MQ=255)
                   acgacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGcc                                                                             >  1:3894594/1‑101 (MQ=255)
                   acgacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGcc                                                                             <  2:782646/101‑1 (MQ=255)
                    cgacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGccc                                                                            >  1:2944480/1‑101 (MQ=255)
                     gacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCg                                                                           <  1:2810739/101‑1 (MQ=255)
                      acCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCgg                                                                          >  2:2152190/1‑100 (MQ=255)
                       ccGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAg                                                                         >  2:3418303/1‑101 (MQ=255)
                         gAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGa                                                                       >  1:17868/1‑101 (MQ=255)
                          aGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGaa                                                                      >  1:4633721/1‑101 (MQ=255)
                          aGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGGCGATGCGGGGGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGaa                                                                      >  2:1544236/1‑101 (MQ=255)
                          aGGGAAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGaa                                                                      >  2:2903361/1‑101 (MQ=255)
                           gggCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAAt                                                                     <  2:3504029/101‑1 (MQ=255)
                           gggCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAAt                                                                     <  1:2069709/101‑1 (MQ=255)
                            ggCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAAtt                                                                    <  2:4399130/101‑1 (MQ=255)
                             gCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTc                                                                   <  2:2494051/101‑1 (MQ=255)
                               aaTGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCgg                                                                 >  2:726564/1‑101 (MQ=255)
                               aaTGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCgg                                                                 >  1:2557945/1‑101 (MQ=255)
                               aaTGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCgg                                                                 <  2:4678799/101‑1 (MQ=255)
                                  gTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgcgc                                                              >  1:953739/1‑101 (MQ=255)
                                  gTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATATGCTCTTGATCACGCCCGAGGAATTCGgcgc                                                              <  1:4262110/101‑1 (MQ=255)
                                   tCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGcc                                                             >  1:2605015/1‑101 (MQ=255)
                                   tCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGcc                                                             <  1:2014654/101‑1 (MQ=255)
                                    cTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCa                                                            >  1:1731631/1‑101 (MQ=255)
                                     tGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCAt                                                           >  2:2433839/1‑101 (MQ=255)
                                      ggATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATc                                                          >  1:748476/1‑101 (MQ=255)
                                       gATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCt                                                         <  2:219384/101‑1 (MQ=255)
                                         tCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGa                                                       <  2:4304703/101‑1 (MQ=255)
                                          cACGCTGAACGGGGTGCATCTGGCCGAGGAGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGAc                                                      >  1:4371382/1‑101 (MQ=255)
                                           tagCTGGACGGGGGGCATCTTGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCGGAGGGTGTGCTCTTGATCGCGCCCGAGGAATTCGGCGCCATCTGAcc                                                     <  1:2350499/99‑1 (MQ=255)
                                              cTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCggg                                                  >  2:2640895/1‑101 (MQ=255)
                                              cTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCggg                                                  >  1:2182769/1‑101 (MQ=255)
                                               tGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGc                                                 <  1:890808/101‑1 (MQ=255)
                                                gAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGcc                                                >  2:384676/1‑101 (MQ=255)
                                                gAACGGGGTGCATCTGGCCGAGGTGGGCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGcc                                                >  1:1194023/1‑101 (MQ=255)
                                                 aaCGGGGTGCATCTGGCAGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCt                                               >  2:805891/1‑101 (MQ=255)
                                                   cGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTgg                                             <  1:3864105/101‑1 (MQ=255)
                                                      ggTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGc                                          <  1:940112/101‑1 (MQ=255)
                                                      ggTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGc                                          >  1:3271457/1‑101 (MQ=255)
                                                        tGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCtt                                        <  1:4355381/101‑1 (MQ=255)
                                                        tGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCtt                                        <  2:164824/101‑1 (MQ=255)
                                                         gCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTc                                       <  2:748402/101‑1 (MQ=255)
                                                         gCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTc                                       <  1:1001810/101‑1 (MQ=255)
                                                            tCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCt                                    >  2:1520897/1‑101 (MQ=255)
                                                              tGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCttt                                  <  2:1201963/101‑1 (MQ=255)
                                                              tGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCttt                                  >  1:3237416/1‑101 (MQ=255)
                                                              tGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCttt                                  <  1:3729160/101‑1 (MQ=255)
                                                               ggCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTg                                 <  2:466466/101‑1 (MQ=255)
                                                               ggCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTg                                 <  1:802994/101‑1 (MQ=255)
                                                               ggCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTg                                 <  2:1334419/101‑1 (MQ=255)
                                                                gCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGc                                <  1:205438/101‑1 (MQ=255)
                                                                   gAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGcccc                             >  2:2398107/1‑101 (MQ=255)
                                                                   gAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGcccc                             >  2:2769429/1‑101 (MQ=255)
                                                                    aGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCg                            <  1:4727477/101‑1 (MQ=255)
                                                                     ggtggtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGc                           >  1:1589863/1‑101 (MQ=255)
                                                                      gtggtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCt                          >  1:3376004/1‑101 (MQ=255)
                                                                       tggtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCTCCGCtt                         >  1:773138/1‑101 (MQ=255)
                                                                       tggtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCtt                         <  2:3204518/101‑1 (MQ=255)
                                                                       tggtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCtt                         <  2:3238530/101‑1 (MQ=255)
                                                                       tggtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCtt                         <  1:3419703/101‑1 (MQ=255)
                                                                        ggtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCttt                        <  2:4525484/101‑1 (MQ=255)
                                                                        ggtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCttt                        <  1:1129055/101‑1 (MQ=255)
                                                                         gtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCtttt                       <  2:456312/101‑1 (MQ=255)
                                                                          tCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTc                      <  2:3605437/101‑1 (MQ=255)
                                                                           cGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTcc                     >  1:1801382/1‑101 (MQ=255)
                                                                           cGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTcc                     >  2:1931662/1‑101 (MQ=255)
                                                                            gATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCg                    <  2:3953479/101‑1 (MQ=255)
                                                                            gATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCg                    >  1:839896/1‑101 (MQ=255)
                                                                                cGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaa                <  2:1834419/101‑1 (MQ=255)
                                                                                 gggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaaa               <  1:3530776/101‑1 (MQ=255)
                                                                                 gggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaaa               <  2:3894352/101‑1 (MQ=255)
                                                                                 gggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaaa               >  1:3946347/1‑101 (MQ=255)
                                                                                 gggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaaa               <  2:205715/101‑1 (MQ=255)
                                                                                 gggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaaa               <  2:1055702/101‑1 (MQ=255)
                                                                                  ggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAc              <  1:450024/101‑1 (MQ=255)
                                                                                  ggCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAc              <  1:3162811/101‑1 (MQ=255)
                                                                                   gCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcg             <  2:4505559/101‑1 (MQ=255)
                                                                                   gCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcg             >  2:3980843/1‑101 (MQ=255)
                                                                                       tCTGCGCCCCTAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgccgc         <  1:99035/101‑1 (MQ=255)
                                                                                       tCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgccgc         >  1:2227109/1‑101 (MQ=255)
                                                                                       tCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgccgc         >  2:2546784/1‑101 (MQ=255)
                                                                                       tCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgccgc         >  1:531040/1‑101 (MQ=255)
                                                                                        cTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACgccgcc        >  1:1670833/1‑101 (MQ=255)
                                                                                         tGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCa       <  2:3268703/101‑1 (MQ=255)
                                                                                           cgcCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATc     <  1:4467893/101‑1 (MQ=255)
                                                                                           cgcCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATc     >  2:4750682/1‑101 (MQ=255)
                                                                                            gcCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATcc    <  1:1352072/101‑1 (MQ=255)
                                                                                             acccGAGGATGTGCTCTTGAGCACGCCCGAGGAATTCGGCGCCATCTGACCGGTCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATccg   <  1:1406905/100‑1 (MQ=255)
                                                                                              cccGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATccgc  >  2:4444421/1‑101 (MQ=255)
                                                                                                 |                                                                                                 
AGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCAAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATCCGC  >  NC_014034/317830‑318024

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG