Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,216,136 C→A 100% A65D (GCT→GAT)  dctS3 → C4‑dicarboxylate transport sensor protein DctS

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,216,1360C→A100.0% 849.6 219A65D (GCT→GAT) dctS3C4‑dicarboxylate transport sensor protein DctS
Reads supporting (aligned to +/- strand):  new base (93/126):  ref base (0/0):  total (93/126)

Only 100 of 238 total aligned reads displayed.

GCTGGTGGCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGCTGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTCGCAA  >  NC_014034/3216038‑3216233
                                                                                                  |                                                                                                 
gCTGGTGGCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCActgatg                                                                                                 <  1:387242/101‑1 (MQ=255)
 cTGGTGGCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACtgatgc                                                                                                <  1:3740552/101‑1 (MQ=255)
   ggtggCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATgcgc                                                                                              <  2:4310955/101‑1 (MQ=255)
    gtggCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCt                                                                                             <  1:3637153/101‑1 (MQ=255)
    gtggCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCt                                                                                             >  1:3517314/1‑101 (MQ=255)
     tggCGATCGTCGCGCTGATGCCGCTGGGCGGCGCGCTCCTCTTCGCCGTCGCGCAATCCGATGCCAGCCGCGCCCGGGCGAAACTGGCCACTGATGCGCtt                                                                                            <  2:4399254/101‑1 (MQ=255)
       gCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTg                                                                                          <  1:3363908/101‑1 (MQ=255)
        cGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTgg                                                                                         >  1:2684114/1‑101 (MQ=255)
         gATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTggg                                                                                        >  2:636998/1‑101 (MQ=255)
          aTCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGt                                                                                       >  1:4544236/1‑101 (MQ=255)
            cgtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCg                                                                                     >  2:829664/1‑101 (MQ=255)
            cgtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCg                                                                                     >  1:1869393/1‑101 (MQ=255)
            cgtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCg                                                                                     >  2:3801157/1‑101 (MQ=255)
            cgtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCg                                                                                     <  2:1569400/101‑1 (MQ=255)
            cgtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCg                                                                                     >  2:2947351/1‑101 (MQ=255)
             gtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGa                                                                                    <  1:3494480/101‑1 (MQ=255)
             gtcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGa                                                                                    <  1:336196/101‑1 (MQ=255)
              tcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGaa                                                                                   <  1:2207487/101‑1 (MQ=255)
              tcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGaa                                                                                   >  2:2454536/1‑101 (MQ=255)
              tcgCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGaa                                                                                   >  1:4414309/1‑101 (MQ=255)
               cgcgCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAAc                                                                                  >  2:2399320/1‑101 (MQ=255)
               cgcgCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAAc                                                                                  <  1:764334/101‑1 (MQ=255)
               cgcgCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAAc                                                                                  <  2:1687060/101‑1 (MQ=255)
                gcgcTGATGGCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACa                                                                                 <  2:2180280/101‑1 (MQ=255)
                gcgcTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACa                                                                                 <  2:1180570/101‑1 (MQ=255)
                 cgcTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAg                                                                                <  1:2305029/101‑1 (MQ=255)
                   cTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGAc                                                                              <  1:1108963/101‑1 (MQ=255)
                     gatgaCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGc                                                                            <  2:438296/101‑1 (MQ=255)
                     gatgaCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGc                                                                            >  2:2028597/1‑101 (MQ=255)
                      atgaCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCt                                                                           <  1:1932597/101‑1 (MQ=255)
                        gaCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTgc                                                                         >  2:3628381/1‑101 (MQ=255)
                         aCGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTgcg                                                                        <  1:1216545/101‑1 (MQ=255)
                          cGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTgcgc                                                                       <  1:1083650/101‑1 (MQ=255)
                            cTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCtt                                                                     >  2:501913/1‑101 (MQ=255)
                              ggTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTc                                                                   <  2:1915225/101‑1 (MQ=255)
                                tCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAg                                                                 >  2:1820393/1‑101 (MQ=255)
                                tCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAg                                                                 <  2:584391/101‑1 (MQ=255)
                                 cGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGa                                                                <  1:548078/101‑1 (MQ=255)
                                  ggCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGAt                                                               >  2:3441508/1‑101 (MQ=255)
                                  ggCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGAt                                                               <  2:4414059/101‑1 (MQ=255)
                                   gcgcgcTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATg                                                              <  2:4699123/101‑1 (MQ=255)
                                    cgcgcTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATgg                                                             <  1:729205/101‑1 (MQ=255)
                                    cgcgcTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATgg                                                             >  2:2874944/1‑101 (MQ=255)
                                      cgcTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCg                                                           >  2:889648/1‑101 (MQ=255)
                                      cctccccctTCCCCGCCGCACATCCCGATGCCAACCGGGCCCGGGGGAAACTGGCCCCTGAGGCGCTTTGGGCCGAACAGACGCTGCGCTTCGAGATGGCg                                                           <  2:1870640/94‑1 (MQ=255)
                                       gctgctCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCgg                                                          >  2:2487940/1‑101 (MQ=255)
                                         tgctCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTg                                                        >  1:2040508/1‑101 (MQ=255)
                                           ctctTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGa                                                      <  1:2924784/101‑1 (MQ=255)
                                            tctTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGAc                                                     >  2:1048247/1‑101 (MQ=255)
                                                cGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAgg                                                 <  1:311240/101‑1 (MQ=255)
                                                 gCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGa                                                <  2:1984880/101‑1 (MQ=255)
                                                  ccGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGAt                                               <  2:3159836/101‑1 (MQ=255)
                                                   cGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGAtg                                              <  2:3131812/101‑1 (MQ=255)
                                                      cgcgCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTAGACGAGGATGTGc                                           >  1:4078521/1‑101 (MQ=255)
                                                        cgcAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTg                                         <  1:1057679/101‑1 (MQ=255)
                                                         gcAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTgg                                        >  1:3788322/1‑101 (MQ=255)
                                                           aaTCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGCCGAGGATGTGCTGGTg                                      >  1:4138359/1‑101 (MQ=255)
                                                           aaTCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTg                                      >  2:1144545/1‑101 (MQ=255)
                                                                 aTGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTg                                >  1:3979667/1‑101 (MQ=255)
                                                                 aTGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTg                                <  1:665021/101‑1 (MQ=255)
                                                                 aTGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTg                                <  1:466763/101‑1 (MQ=255)
                                                                 aTGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTg                                <  1:4200042/101‑1 (MQ=255)
                                                                  tGCCAACCGCGCCCGGGCGAAACTGGCCACTTATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTTGACGAGGATGTGCTGGTGCGTCTgg                               <  1:3237553/101‑1 (MQ=255)
                                                                  tGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTgg                               <  1:1802422/101‑1 (MQ=255)
                                                                  tGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTgg                               <  2:4136942/101‑1 (MQ=255)
                                                                  tGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTgg                               <  1:829737/101‑1 (MQ=255)
                                                                  tGCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTgg                               >  2:2561189/1‑101 (MQ=255)
                                                                   gCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGgc                              >  1:972527/1‑101 (MQ=255)
                                                                   gCCAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGgc                              >  1:4739205/1‑101 (MQ=255)
                                                                     cAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGgcgc                            <  1:2015070/101‑1 (MQ=255)
                                                                     cAACCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGgcgc                            >  1:3344127/1‑101 (MQ=255)
                                                                       aCCGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTg                          <  1:1451033/101‑1 (MQ=255)
                                                                        ccGCGCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTgg                         <  1:4359765/101‑1 (MQ=255)
                                                                         cgcgCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGa                        >  1:4648200/1‑101 (MQ=255)
                                                                         cgcgCCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGa                        <  1:292581/101‑1 (MQ=255)
                                                                           cgcCCGGGCGAAACTGGCCACTGATGCGCGTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATg                      <  1:2138612/101‑1 (MQ=255)
                                                                            gcCCGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGc                     <  1:2545381/101‑1 (MQ=255)
                                                                             cccGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGAGGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGcc                    <  1:144743/101‑1 (MQ=255)
                                                                              ccGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCa                   <  2:1798466/101‑1 (MQ=255)
                                                                              ccGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCa                   <  1:1678802/101‑1 (MQ=255)
                                                                              ccGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCa                   <  1:2412460/101‑1 (MQ=255)
                                                                              ccGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCa                   <  2:4031770/101‑1 (MQ=255)
                                                                               cGGGCGAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAg                  >  2:144175/1‑101 (MQ=255)
                                                                                 ggCGAAACTGGCCACTGGTGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAgcg                <  2:3467747/101‑1 (MQ=255)
                                                                                    gAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCg             <  2:3284801/101‑1 (MQ=255)
                                                                                    gAAACTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGCGGACGAGGATGAGCTGGTGCGTCTGGCGCTGGTTGCCAGCGCCg             >  2:4295394/1‑101 (MQ=255)
                                                                                     aaaCTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCgg            >  2:1113720/1‑101 (MQ=255)
                                                                                     aaaCTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCgg            <  1:2850290/101‑1 (MQ=255)
                                                                                        cTGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGc         <  1:1853875/101‑1 (MQ=255)
                                                                                         tGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGcc        <  2:1501836/101‑1 (MQ=255)
                                                                                         tGGCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGcc        >  2:4484506/1‑101 (MQ=255)
                                                                                           gCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTc      <  2:1537023/101‑1 (MQ=255)
                                                                                           gCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTc      <  2:3688584/101‑1 (MQ=255)
                                                                                           gCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTc      <  1:993740/101‑1 (MQ=255)
                                                                                           gCCACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTc      <  2:3052719/101‑1 (MQ=255)
                                                                                             cACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTCGc    >  2:392918/1‑101 (MQ=255)
                                                                                             cACTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTCGc    <  2:2591734/101‑1 (MQ=255)
                                                                                              aCTGATGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGGGCGTCTGGCGCTGGAGGCCAGCGCCGGGGCCTCGCa   <  1:849419/101‑1 (MQ=255)
                                                                                               ctgatgCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTCGCaa  <  1:3801392/101‑1 (MQ=255)
                                                                                               ctgatgCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTCGCaa  >  2:1215790/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GCTGGTGGCGATCGTCGCGCTGATGACGCTGGTCGGCGCGCTGCTCTTCGCCGTCGCGCAATCCGATGCCAACCGCGCCCGGGCGAAACTGGCCACTGCTGCGCTTTGGGTCGAACAGACGCTGCGCTTTCAGATGGCGGTGGACGAGGATGTGCTGGTGCGTCTGGCGCTGGATGCCAGCGCCGGGGCCTCGCAA  >  NC_014034/3216038‑3216233

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG