Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,223,935	G→C	100%	P129A (CCC→GCC)	fdhD ←	formate dehydrogenase accessory protein FdhD

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,223,935	0	G→C	100.0%	64.9	17	P129A (CCC→GCC)	fdhD	formate dehydrogenase accessory protein FdhD
Reads supporting (aligned to +/- strand):  new base (0/17):  ref base (0/0):  total (0/17)

CGCCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGGGGGGGCCCCGCCCCGGGGCGCCCGCGGCAGCGGGCGCAACACAGCCGCCAGGCTGTCAAGGCCGCAAAGCCCGCAGCCGACCGGACCGGCCA  >  NC_014034/3223839‑3224027                                                                                                 |                                                                                             cgcCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcgggg                                                                                          <  1:1974566/101‑1 (MQ=255)   ccGTCCCAGAACCCCGCCGCATGCACGGCCCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgc                                                                                        <  2:3969552/101‑1 (MQ=255)   ccGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgc                                                                                        <  1:844309/101‑1 (MQ=255)      tCCCAGACCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcc                                                                                     <  1:2345705/101‑1 (MQ=255)       cccAGAACCCCGCCTCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcca                                                                                    <  1:4095393/101‑2 (MQ=255)       cccAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCACGCGCTCCATCGGCCAGGGGGGCGGGGgcgcca                                                                                    <  1:1231408/101‑2 (MQ=255)       cccAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcca                                                                                    <  1:680305/101‑2 (MQ=255)        ccAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaa                                                                                   <  2:4010616/101‑3 (MQ=255)        ccAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaa                                                                                   <  2:344619/101‑3 (MQ=255)        caaGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaa                                                                                   <  1:3325404/99‑3 (MQ=255)          aGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagc                                                                                 <  1:39496/101‑5 (MQ=255)          aGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagc                                                                                 <  2:3127074/101‑5 (MQ=255)              ccccGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgcca                                                                             <  2:4716768/101‑9 (MQ=255)              ccccGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgcca                                                                             <  1:4541571/101‑9 (MQ=255)              ccccGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgcca                                                                             <  1:3034947/101‑9 (MQ=255)               cccGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgccag                                                                            <  1:1790708/101‑10 (MQ=255)               cccGCCGCAGGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgccag                                                                            <  2:4398490/101‑10 (MQ=255)                ccgccgCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgccagc                                                                           <  1:341921/101‑11 (MQ=255)                                                                                         cgccggcggggcgcCCCCGCCCCGGGGCGCCCGCGGCAGCGGGCGCAACACAGCCGCCAGGCTGTCAAGGCCGCAAAGCCCGCAGCCGACCGGACCGGCCa  <  1:4666137/94‑1 (MQ=255)                                                                                                 |                                                                                             CGCCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGGGGGGGCCCCGCCCCGGGGCGCCCGCGGCAGCGGGCGCAACACAGCCGCCAGGCTGTCAAGGCCGCAAAGCCCGCAGCCGACCGGACCGGCCA  >  NC_014034/3223839‑3224027 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG