Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,204 T→C 100% C449C (TGT→TGC recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,2040T→C100.0% 362.1 98C449C (TGT→TGCrecJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (37/61):  ref base (0/0):  total (37/61)

Only 100 of 111 total aligned reads displayed.

GCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGA.GG.GCA.G.TGGA.A.CCGCGATGG.AA.GGCT.GCCG.A.G.TGTTCGCCCGGCAGGGC.G.C.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCG  >  NC_014034/3256117‑3256292
                                                                                                    |                                                                                            
gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGccc                                                                                        >  1:2414166/1‑101 (MQ=255)
 cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCg                                                                                       <  1:2242462/101‑1 (MQ=255)
  ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGccccg                                                                                      >  2:1787309/1‑99 (MQ=255)
  ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCgg                                                                                      >  2:2374272/1‑101 (MQ=255)
  ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCgg                                                                                      >  2:2359202/1‑101 (MQ=255)
  ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCgc                                                                                      >  2:2076447/1‑100 (MQ=255)
   tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAGC.CCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGc                                                                                     <  2:437332/101‑1 (MQ=255)
     tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCGCG.G.TGCTCGCCCGGCAg                                                                                   >  2:2658248/1‑101 (MQ=255)
      gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAgg                                                                                  >  2:1352101/1‑101 (MQ=255)
      gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCCCGCCCGGCGgg                                                                                  >  2:4686789/1‑101 (MQ=255)
        tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc                                                                                >  1:350947/1‑101 (MQ=255)
        tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCCCCCGGCAgggg                                                                                >  1:1706393/1‑100 (MQ=255)
        tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGG.CACGGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc                                                                                <  1:979901/101‑1 (MQ=255)
        tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGG.CACGGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc                                                                                <  2:1754536/101‑1 (MQ=255)
         cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAggggg                                                                               >  1:2802289/1‑99 (MQ=255)
         cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc.g                                                                              <  2:1474308/101‑1 (MQ=255)
          aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc.g.c                                                                            >  1:2440977/1‑101 (MQ=255)
          aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGCA.ACCCGCGATGGCAC.GGCTGGCCG.AGG.TACTCGCCCGGCCGGgg.g.c                                                                            >  1:2166548/1‑101 (MQ=255)
           tctgcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGc.g.c.g                                                                          <  1:2186548/98‑1 (MQ=255)
           aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAggggggg                                                                             >  1:4192183/1‑97 (MQ=255)
             ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCCCCCCGCAGGGC.G.C.ggg                                                                        >  2:2790294/1‑100 (MQ=255)
              gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGG.GCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCCGGGc.g.c.gcgg                                                                       >  2:2186424/1‑98 (MQ=255)
              gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.C.gggc                                                                       <  1:2854973/101‑3 (MQ=255)
               cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGGTCGCCCGGCAGGgc.ggcgggc                                                                        >  1:4001771/1‑95 (MQ=255)
               cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGGAGGGC.G.C.ggggc                                                                      >  1:378473/1‑98 (MQ=255)
                ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCATC.TGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.C.gggccc                                                                     >  1:1496045/1‑97 (MQ=255)
                ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.C.ggggac                                                                     >  2:2921778/1‑97 (MQ=255)
                ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.C.gggcac                                                                     <  2:1499717/101‑5 (MQ=255)
                 gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGc.g.c.gatcagc                                                                    <  2:2466140/101‑7 (MQ=255)
                 gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.C.gggcacg                                                                    <  1:751831/101‑6 (MQ=255)
                  cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGATGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCAc.gg                                                                   >  2:4341233/1‑101 (MQ=255)
                     gCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGG.G.GGCAc.ggggc                                                                >  1:4719768/1‑98 (MQ=255)
                     gCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCAc.ggggg                                                                >  1:2039066/1‑101 (MQ=255)
                      cACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCAc.gggcgg                                                               <  1:1431487/101‑5 (MQ=255)
                      cACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCAc.gggcgg                                                               <  1:1352193/101‑5 (MQ=255)
                       aCCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGG.G.CGGGCAc.gggcggc                                                              >  2:1409502/1‑96 (MQ=255)
                         cGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggccc                                                             <  1:1942357/101‑1 (MQ=255)
                          gCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccg                                                            <  2:1396245/101‑1 (MQ=255)
                          gCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccg                                                            <  2:1920817/101‑1 (MQ=255)
                           cATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccgg                                                           <  2:3190852/101‑1 (MQ=255)
                            aTGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGG.G.CGGGCACGGGcgggccggc                                                          >  1:1063846/1‑101 (MQ=255)
                            aTGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccggc                                                          <  2:1356459/101‑1 (MQ=255)
                            aTGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCACGGC.G.CGGGCAc.gggccggccggc                                                         >  2:4177494/1‑91 (MQ=255)
                             tGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccggcc                                                         <  2:2327371/101‑1 (MQ=255)
                             tGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccggcc                                                         <  1:2242332/101‑1 (MQ=255)
                             tGGCCGCGGGGCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCCC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccggcc                                                         <  1:535005/101‑1 (MQ=255)
                             gggCCGCGGGTCTCGCCCTGGCCGA.GGCGCA.GCAGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccggcc                                                         <  1:4082222/100‑1 (MQ=255)
                              ggCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCg                                                        <  2:2595281/101‑1 (MQ=255)
                              ggCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCg                                                        <  1:3054207/101‑1 (MQ=255)
                              ggCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCg                                                        <  2:478590/101‑1 (MQ=255)
                               gCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.GCC.GGGACGGGCGGCCCGGCCgg                                                       >  2:4608852/1‑100 (MQ=255)
                                ccGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGAt                                                      <  2:1375646/101‑1 (MQ=255)
                                 cgcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGGTc                                                     >  1:3459888/1‑101 (MQ=255)
                                 cgcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGGTc                                                     >  1:3798680/1‑101 (MQ=255)
                                 cgcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATc                                                     <  1:264196/101‑1 (MQ=255)
                                 cgcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATc                                                     <  1:2658402/101‑1 (MQ=255)
                                  gcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGGGGGCCGGGCGATCt                                                    >  2:1408251/1‑101 (MQ=255)
                                  gcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCt                                                    <  2:3511752/101‑1 (MQ=255)
                                    gggTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGc                                                  >  2:1016674/1‑101 (MQ=255)
                                      gTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCgg                                                <  1:4687045/101‑1 (MQ=255)
                                        cTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCt                                              <  1:2040909/101‑1 (MQ=255)
                                        cTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCt                                              <  1:3157167/101‑1 (MQ=255)
                                         tGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                                             <  2:350913/101‑1 (MQ=255)
                                         tGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                                             <  2:3522607/101‑1 (MQ=255)
                                         tGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCCATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                                             >  1:4227446/1‑101 (MQ=255)
                                           aCCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGa                                           <  2:1738096/101‑1 (MQ=255)
                                           aCCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGa                                           <  1:1209142/101‑1 (MQ=255)
                                            ccctggCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                                          <  1:178779/96‑1 (MQ=255)
                                            ccctggCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                                          <  2:2972853/96‑1 (MQ=255)
                                              ctggCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCgg                                        >  1:486038/4‑101 (MQ=255)
                                              ctggCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACgg                                        <  1:4655592/98‑1 (MQ=255)
                                               tggCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGG.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCCggggggg                                       >  1:3757425/3‑96 (MQ=255)
                                               tggCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACggg                                       <  2:2517356/99‑1 (MQ=255)
                                                 gCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt                                     <  1:3318283/101‑1 (MQ=255)
                                                       aggcgcagctGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                                 <  2:512892/92‑1 (MQ=255)
                                                        ggcgcagctGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                <  1:2790459/93‑1 (MQ=255)
                                                          cgcagctGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATg                              >  2:2748577/7‑101 (MQ=255)
                                                             agctGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCg                           <  2:3782793/98‑1 (MQ=255)
                                                              gctGGA.ACCCGCGATGG.CACGGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCgg                          >  1:3937234/3‑101 (MQ=255)
                                                               ctGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg                         <  1:1149062/100‑1 (MQ=255)
                                                                tGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGgc                        >  2:3740273/1‑101 (MQ=255)
                                                                  ggaacccGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGGCGGGCCGGGTCCCGATGGCggggg                       >  1:173729/6‑99 (MQ=255)
                                                                   gaacccGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTTGGCGGGGCCCCGATGCCGGgcgc                      >  1:2020287/5‑101 (MQ=255)
                                                                   gaacccGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGGTGGCGGgggc                      >  1:2379455/5‑101 (MQ=255)
                                                                    gccccGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                     <  2:4191937/98‑1 (MQ=255)
                                                                      cccGCGATGGCAC.GGCTGGCCG.A.GTTGCTGGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccgc                   <  2:2166424/100‑1 (MQ=255)
                                                                        cgcgATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGCGCCCCGa                 >  1:1990691/1‑101 (MQ=255)
                                                                        cgcgATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGa                 <  1:2359335/101‑1 (MQ=255)
                                                                         gcgATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc                <  1:3020521/101‑1 (MQ=255)
                                                                                gcacggctggCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCcgc          <  1:1016753/92‑1 (MQ=255)
                                                                                   cggctggCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCt       <  1:2472941/95‑1 (MQ=255)
                                                                                    ggctggCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTg      <  2:3973209/96‑1 (MQ=255)
                                                                                    ggctggCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTg      <  2:2220878/96‑1 (MQ=255)
                                                                                      ctggCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGAt    <  2:1097841/98‑1 (MQ=255)
                                                                                       tggCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc   >  1:1476026/3‑101 (MQ=255)
                                                                                       tggCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCgcgccggtc   >  1:4032665/3‑96 (MQ=255)
                                                                                       tggCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc   <  1:4711787/99‑1 (MQ=255)
                                                                                        ggCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg  <  2:3798445/100‑1 (MQ=255)
                                                                                        ggCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg  >  1:2183718/2‑101 (MQ=255)
                                                                                        ggCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg  >  2:2199190/2‑101 (MQ=255)
                                                                                                    |                                                                                            
GCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGA.GG.GCA.G.TGGA.A.CCGCGATGG.AA.GGCT.GCCG.A.G.TGTTCGCCCGGCAGGGC.G.C.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCG  >  NC_014034/3256117‑3256292

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG