Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,333,494	C→G	100%	intergenic (+40/‑126)	ccmH → / → paaG	cytochrome c‑type biogenesis protein CcmH/enoyl‑CoA hydratase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,333,494	0	C→G	100.0%	79.8	22	intergenic (+40/‑126)	ccmH/paaG	cytochrome c‑type biogenesis protein CcmH/enoyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  new base (0/22):  ref base (0/0):  total (0/22)

GCCGGTGCTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGCGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGCCCGCGCG  >  NC_014034/3333400‑3333591                                                                                               |                                                                                                  gccggtgcTCTGGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTcc                                                                                             <  1:3312074/101‑1 (MQ=255) gccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTcc                                                                                             <  2:90973/101‑1 (MQ=255)  ccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCg                                                                                            <  1:4497509/101‑1 (MQ=255)     gtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGTTTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGccc                                                                                         <  1:1627321/101‑1 (MQ=255)        ctctCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcccccc                                                                                      <  1:1895369/101‑1 (MQ=255)            cGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAc                                                                                  <  1:3150800/101‑1 (MQ=255)              gCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAccc                                                                                <  1:630618/101‑1 (MQ=255)               ccGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCGGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAcccc                                                                               <  1:3520149/101‑1 (MQ=255)               ccGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAcccc                                                                               <  1:223567/101‑1 (MQ=255)                cGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAccccc                                                                              <  2:3266642/101‑1 (MQ=255)                cGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAccccc                                                                              <  1:2817699/101‑1 (MQ=255)                 gAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAcccccc                                                                             <  1:4589564/101‑1 (MQ=255)                     aaGAGGCGCGGCTAAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGCCTTGCGCACCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGc                                                                         <  2:4175572/101‑1 (MQ=37)                          gggcgGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCGGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTAtt                                                                    <  1:1175947/99‑1 (MQ=255)                               gCTGAAAGGCCGGCGCAAGGACTAGGGATTCCCCCCGGGCCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGcc                                                               <  2:2988947/101‑1 (MQ=21)                               gCTGAAAGCCCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGcc                                                               <  1:3857459/101‑1 (MQ=255)                                                      aGGGATTTCGCCCGGGGCTTCCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCg                                        <  1:2099894/101‑1 (MQ=255)                                                          gtttCGCCCGGGGCTTGCGCCCGGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGAc                                    <  2:734996/100‑1 (MQ=255)                                                           ttccGCCGGGGGCTGGCGCCCGGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACg                                   <  2:4044114/98‑1 (MQ=18)                                                                     ggCTTGCGCCCCGGGCGCCCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCg                         <  1:59634/101‑1 (MQ=255)                                                                          gcgcCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTg                    <  2:996397/101‑1 (MQ=255)                                                                                 gggCGCCCCGCGGGGGGCTCCGCCCCCCCCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCg             <  1:2166012/101‑1 (MQ=255)                                                                                            ggggggCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGCCcgcgcg  >  1:499585/1‑101 (MQ=255)                                                                                            ggggggCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGGCTGGGCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGCCcgcgcg  >  1:76783/1‑101 (MQ=255)                                                                                            ggggggCTCCGCCCCCCCCACCCCCCGGGCGGATTTTGCCAAGGAAAAGCGGGGGGCTGGTCGTGACGTTTGGGTTCGCTTTGCCTTTCCGCGCCcgcgcg  >  2:2098271/1‑101 (MQ=25)                                                                                               |                                                                                                  GCCGGTGCTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGCGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTCGGCGCCCGCGCG  >  NC_014034/3333400‑3333591 Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG