Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,342,563	A→C	100%	L155R (CTG→CGG)	RCAP_rcc03145 ←	AsnC/Lrp family transcriptional regulator

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,342,563	0	A→C	100.0%	77.0	21	L155R (CTG→CGG)	RCAP_rcc03145	AsnC/Lrp family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (0/21):  ref base (0/0):  total (0/21)

AGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACACCCAGCACCGGGGCAGCCGCGGCCGCGGGTTGCGGCAGGACAGCTG  >  NC_014034/3342465‑3342594                                                                                                   |                                cgCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgc                               <  2:706328/100‑1 (MQ=255)   cGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcgg                             <  2:1612640/101‑3 (MQ=255)     tctcGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcg                           <  2:2837474/101‑1 (MQ=255)     tctcGGGGCTGATCTCGTTCAGCGAGGGTTGAGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcg                           <  1:3101484/101‑1 (MQ=255)       tcGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggc                         <  1:1588647/101‑1 (MQ=255)        cGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggcc                        <  2:4251061/101‑1 (MQ=255)        cGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCCCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggcc                        >  1:2529222/1‑101 (MQ=255)         ggggCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggccg                       <  2:378088/101‑1 (MQ=255)         ggggCTGATCCCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCAGCGGGGCCgcggcggccg                       <  2:4010851/101‑1 (MQ=255)           ggCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggccgcg                     <  2:710791/101‑1 (MQ=255)             cTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCggg                   <  1:2883819/101‑1 (MQ=255)              tGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGt                  <  2:3137728/101‑1 (MQ=255)               gATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGGGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGtt                 <  2:4318949/101‑1 (MQ=255)                aTCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTg                <  1:4641231/101‑1 (MQ=255)                aTCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTg                <  2:4724903/101‑1 (MQ=255)                 tctcGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGc               <  1:3570543/101‑1 (MQ=255)                      ttCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcgg          <  1:3346764/101‑3 (MQ=255)                       tCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGGCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggc         <  2:2256068/101‑4 (MQ=255)                       tCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggc         <  2:2529077/101‑4 (MQ=255)                       tCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggc         <  2:2660486/101‑4 (MQ=255)                          gggAGGGTTGCGGCGGGAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcacc      <  1:2081968/99‑7 (MQ=255)                              gggTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccggcg  <  1:2659787/101‑11 (MQ=255)                                                                                                   |                                AGCGTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACACCCAGCACCGGGGCAGCCGCGGCCGCGGGTTGCGGCAGGACAGCTG  >  NC_014034/3342465‑3342594 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 38 ≤  ATCG