Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,398,880 G→A 100% P228L (CCG→CTG)  tolQ ← TolQ protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,398,8800G→A100.0% 938.1 244P228L (CCG→CTG) tolQTolQ protein
Reads supporting (aligned to +/- strand):  new base (109/135):  ref base (0/0):  total (109/135)

Only 100 of 259 total aligned reads displayed.

CGGCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGC  >  NC_014034/3398782‑3398977
                                                                                                  |                                                                                                 
cGGCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACagg                                                                                                 <  2:51588/101‑1 (MQ=255)
 ggCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGa                                                                                                <  1:1877306/101‑1 (MQ=255)
  gCATGTTTGCCCTTGCCGCCGCGGCGGTGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGAt                                                                                               >  1:1261825/1‑101 (MQ=255)
  gCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGAt                                                                                               <  1:1772652/101‑1 (MQ=255)
  gCATGTTGGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGAt                                                                                               <  2:3077480/101‑1 (MQ=255)
   cATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATg                                                                                              <  1:297628/101‑1 (MQ=255)
      gTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTc                                                                                           <  1:2001402/101‑1 (MQ=255)
      gTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTc                                                                                           <  1:4582453/101‑1 (MQ=255)
       tttGCCCTTGCCGCCGCGGCGGCGTTGGCTGCCGCCCCCGCCCTTTTTCATCACCCCCGCGCCCATCCCTTACGCGACCACCTGGGGCGACAGGATGGGCg                                                                                          >  1:1447367/1‑101 (MQ=255)
       tttGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGGCCAGCTGGCGCGACACGAGGGTCg                                                                                          >  2:1655523/1‑101 (MQ=255)
        ttGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCga                                                                                         >  1:3509059/1‑101 (MQ=255)
        ttGCCCTGGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCga                                                                                         <  1:3176/101‑1 (MQ=255)
          gCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCgaga                                                                                       <  2:555382/101‑1 (MQ=255)
            ccTTGCCGCCGCGGCGGCGTCGGCCGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAAt                                                                                     <  1:3845494/101‑1 (MQ=255)
             cgtGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCGCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAAtt                                                                                    <  2:4394662/99‑1 (MQ=255)
                gccgccGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtcgt                                                                                 <  2:140299/101‑1 (MQ=255)
                 ccgccgCGGCGGCCTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtcgtc                                                                                <  2:3723389/101‑1 (MQ=255)
                  cgccgcGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtcgtcg                                                                               <  1:4354727/101‑1 (MQ=255)
                    ccgcGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGCCGGc                                                                             <  1:1553075/101‑1 (MQ=255)
                     cgcgGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCg                                                                            <  2:2247856/101‑1 (MQ=255)
                      gcggcggcgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGa                                                                           >  1:1699748/1‑101 (MQ=255)
                           ggcgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGcc                                                                      >  1:4622155/1‑101 (MQ=255)
                            gcgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCt                                                                     <  2:3177379/101‑1 (MQ=255)
                             cgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCCTCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTc                                                                    >  1:4751624/1‑101 (MQ=255)
                              gTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCg                                                                   <  2:2429488/101‑1 (MQ=255)
                               gcGGCTGCCGCCTCCGCCCTTTTTCATCCCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGt                                                                  <  1:887155/100‑1 (MQ=255)
                                cGGCTGCCGCCTCCGCCCTTTTTCATCCCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTa                                                                 <  1:2738557/101‑1 (MQ=255)
                                cGGCTGCCGCCTCCGCCCTTTTTCATCCCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTa                                                                 <  1:637791/101‑1 (MQ=255)
                                cGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTa                                                                 >  1:1435688/1‑101 (MQ=255)
                                cGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTa                                                                 <  1:4652865/101‑1 (MQ=255)
                                cGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTa                                                                 <  1:3228002/101‑1 (MQ=255)
                                    tGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGccc                                                             >  2:2759766/1‑101 (MQ=255)
                                    tGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGccc                                                             >  2:2501010/1‑101 (MQ=255)
                                    tGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGccc                                                             >  1:2312838/1‑101 (MQ=255)
                                     gccgccTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCg                                                            <  1:3417471/101‑1 (MQ=255)
                                       cgccTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAg                                                          <  1:565512/101‑1 (MQ=255)
                                         ccTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGt                                                        <  2:1005837/101‑1 (MQ=255)
                                            ccGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGAt                                                     >  1:3034372/1‑101 (MQ=255)
                                            ccGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGAt                                                     <  2:1436554/101‑1 (MQ=255)
                                             cGCCCTTTTTCATCCCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGAtc                                                    <  1:3488165/101‑1 (MQ=255)
                                              gCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGAtct                                                   >  1:1028373/1‑101 (MQ=255)
                                                ccTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGAtcttg                                                 <  1:2446997/101‑3 (MQ=255)
                                                 cTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTACCCCGAGGTGATCttgt                                                >  2:398342/1‑101 (MQ=255)
                                                   ttttCATCCCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtcg                                              <  2:1023504/101‑1 (MQ=255)
                                                   ttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGGGGTGATCttgtcg                                              >  1:3707434/1‑101 (MQ=255)
                                                   ttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtcg                                              <  2:1216671/101‑1 (MQ=255)
                                                    tttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcgg                                             >  2:932912/1‑101 (MQ=255)
                                                       catcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgt                                          >  1:2385774/1‑101 (MQ=255)
                                                        ctcccccccGCGCCCGTGGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTGGTAGCCCGAGGTGATCTtgtcggcgtc                                         <  2:4645074/97‑1 (MQ=255)
                                                        atcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtc                                         >  2:3102437/1‑101 (MQ=255)
                                                        atcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtc                                         >  1:1365817/1‑101 (MQ=255)
                                                        atcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGGGATCTtgtcggcgtc                                         >  1:3586407/1‑101 (MQ=255)
                                                         tcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcg                                        >  1:719289/1‑101 (MQ=255)
                                                          caCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcgg                                       >  2:3785515/1‑101 (MQ=255)
                                                           ccccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGa                                      <  1:2242514/100‑1 (MQ=255)
                                                           aCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcggc                                      >  1:1258088/1‑100 (MQ=255)
                                                           aCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGa                                      >  1:3667078/1‑101 (MQ=255)
                                                            cccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGAc                                     >  2:3212526/1‑101 (MQ=255)
                                                             ccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACg                                    >  1:1564899/1‑101 (MQ=255)
                                                               ccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgag                                  >  2:3263590/1‑101 (MQ=255)
                                                               ccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgag                                  <  2:1144814/101‑1 (MQ=255)
                                                                cgcgCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgaga                                 <  2:2963179/101‑1 (MQ=255)
                                                                cgcgCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgaga                                 <  1:3983209/101‑1 (MQ=255)
                                                                 gcgcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGagag                                >  2:4177323/1‑101 (MQ=255)
                                                                   gcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCt                              >  1:3312773/1‑101 (MQ=255)
                                                                    cccATCGCTTACGCGTCCGGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCtt                             >  1:4572798/1‑101 (MQ=255)
                                                                    cccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCtt                             <  2:1291928/101‑1 (MQ=255)
                                                                        tCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgttg                         >  2:408787/1‑101 (MQ=255)
                                                                        tCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgttg                         <  2:1592102/101‑1 (MQ=255)
                                                                        tCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCATGTCGGCGTCGGACGAGAGCttgttg                         >  2:770514/1‑101 (MQ=255)
                                                                        tCGCTGACGCCTCGAGCTGGCGCGGCAGGATGGTCGAGACTTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgttg                         <  2:4009544/101‑1 (MQ=255)
                                                                         cGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTtgttgt                        >  2:4536998/1‑101 (MQ=255)
                                                                          gCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTa                       <  1:1202627/101‑1 (MQ=255)
                                                                           cTTGCGCGTCCAGCGTGCGCGACAGGATGGTCGAGAATTCGGCGGCGAAGGCCTCGTAGCCCGAGGTGACCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                      <  2:2363727/101‑1 (MQ=255)
                                                                           cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                      <  2:825253/101‑1 (MQ=255)
                                                                           cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                      >  1:4447997/1‑101 (MQ=255)
                                                                            ttACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAgg                     >  1:1099536/1‑101 (MQ=255)
                                                                             tACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGc                    <  2:4595274/101‑1 (MQ=255)
                                                                                gcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAt                 >  2:3458427/1‑101 (MQ=255)
                                                                                 cgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATg                <  2:1987575/101‑1 (MQ=255)
                                                                                  gTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAtca               >  1:1148529/1‑99 (MQ=255)
                                                                                  gTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGa               <  2:4744868/101‑1 (MQ=255)
                                                                                  gTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGa               <  1:2491512/101‑1 (MQ=255)
                                                                                   tCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc              <  2:2587040/101‑1 (MQ=255)
                                                                                   gccAGCTGGCGCGACAGGATGGTCGAGAATGCGGCGGCGGAGTCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc              <  2:1874007/100‑1 (MQ=255)
                                                                                     cAGCTGGCGCGACAGGATGGTCGAGAATTCGTCTGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccg            <  1:4106892/101‑1 (MQ=255)
                                                                                     cAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccg            >  2:3343777/1‑101 (MQ=255)
                                                                                     cAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccg            <  1:533849/101‑1 (MQ=255)
                                                                                      aGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgc           >  2:2120227/1‑101 (MQ=255)
                                                                                       gCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgcc          <  1:3435002/101‑1 (MQ=255)
                                                                                        cTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgccg         <  1:1923118/101‑1 (MQ=255)
                                                                                        cTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgccg         <  2:4610343/101‑1 (MQ=255)
                                                                                         tGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCgg        >  2:227817/1‑101 (MQ=255)
                                                                                         tGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCgg        <  1:2505501/101‑1 (MQ=255)
                                                                                            cgcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGAt     <  2:689812/101‑1 (MQ=255)
                                                                                             gcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc    <  2:3521761/101‑1 (MQ=255)
                                                                                             gcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc    <  2:1791872/101‑1 (MQ=255)
                                                                                              cgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcg   >  1:1719047/1‑101 (MQ=255)
                                                                                               gACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcgc  >  2:3654919/1‑101 (MQ=255)
                                                                                               gACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcgc  >  2:3413035/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CGGCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGC  >  NC_014034/3398782‑3398977

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG