Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,398,929 C→T 100% E212K (GAG→AAG)  tolQ ← TolQ protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,398,9290C→T100.0% 807.8 204E212K (GAG→AAG) tolQTolQ protein
Reads supporting (aligned to +/- strand):  new base (109/95):  ref base (0/0):  total (109/95)

Only 100 of 247 total aligned reads displayed.

CTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCGGC  >  NC_014034/3398831‑3399026
                                                                                                  |                                                                                                 
cTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTACCCCGAGGTGATCttgt                                                                                                 >  2:398342/1‑101 (MQ=255)
 tttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtc                                                                                                <  1:4640944/101‑1 (MQ=255)
  ttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtcg                                                                                               <  2:1216671/101‑1 (MQ=255)
   tttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcgg                                                                                              >  2:444465/1‑101 (MQ=255)
    ttCATCCCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggc                                                                                             <  2:547264/101‑1 (MQ=255)
    ttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggc                                                                                             >  1:1592201/1‑101 (MQ=255)
    ttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggc                                                                                             >  1:1987692/1‑101 (MQ=255)
     tcatcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcg                                                                                            >  1:1839234/1‑101 (MQ=255)
     tcatcaCCCCCGCGCCCATCGCTTACCCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGGCTCGTAGCCCGAGGCGATCTtgtcggcg                                                                                            >  1:915036/1‑101 (MQ=255)
      catcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgt                                                                                           >  1:1220025/1‑101 (MQ=255)
       atcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtc                                                                                          >  1:272701/1‑101 (MQ=255)
       atcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtc                                                                                          >  1:1365817/1‑101 (MQ=255)
       atcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGGGATCTtgtcggcgtc                                                                                          >  1:3586407/1‑101 (MQ=255)
        tcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcg                                                                                         >  2:2491368/1‑101 (MQ=255)
        tcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcg                                                                                         >  1:4509967/1‑101 (MQ=255)
        tcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcg                                                                                         >  1:719289/1‑101 (MQ=255)
         caCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcgg                                                                                        >  2:3785515/1‑101 (MQ=255)
          aCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcggc                                                                                       >  1:1258088/1‑100 (MQ=255)
           cccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGAc                                                                                      >  1:2265763/1‑101 (MQ=255)
              ccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgag                                                                                   <  2:1144814/101‑1 (MQ=255)
              ccGAGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgag                                                                                   <  1:3885530/101‑1 (MQ=255)
               cgcgCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgaga                                                                                  >  2:3079388/1‑101 (MQ=255)
               cgcgCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgaga                                                                                  <  1:3983209/101‑1 (MQ=255)
               cgcgCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgaga                                                                                  <  2:2963179/101‑1 (MQ=255)
               cgcgCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgaga                                                                                  <  2:2529718/101‑1 (MQ=255)
                   cccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCtt                                                                              <  2:1291928/101‑1 (MQ=255)
                    ccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttg                                                                             >  1:1925967/1‑101 (MQ=255)
                       tCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTATCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgttg                                                                          >  1:369289/1‑101 (MQ=255)
                       tCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgttg                                                                          >  2:408787/1‑101 (MQ=255)
                       tCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgttg                                                                          <  2:1592102/101‑1 (MQ=255)
                       tCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCATGTCGGCGTCGGACGAGAGCttgttg                                                                          >  2:770514/1‑101 (MQ=255)
                         gCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTa                                                                        <  2:3561098/101‑1 (MQ=255)
                          cTTGCGCGTCCAGCGTGCGCGACAGGATGGTCGAGAATTCGGCGGCGAAGGCCTCGTAGCCCGAGGTGACCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                                                                       <  2:2363727/101‑1 (MQ=255)
                          cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                                                                       <  2:3330916/101‑1 (MQ=255)
                           ttACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAgg                                                                      >  2:71443/1‑101 (MQ=255)
                            tACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGc                                                                     <  2:4595274/101‑1 (MQ=255)
                               gcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAt                                                                  >  2:3458427/1‑101 (MQ=255)
                               gcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAt                                                                  <  1:2501154/101‑1 (MQ=255)
                               gcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAt                                                                  >  1:2405177/1‑101 (MQ=255)
                               gcgCCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAt                                                                  <  2:3216205/101‑1 (MQ=255)
                                cgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATg                                                                 >  2:1006454/1‑101 (MQ=255)
                                 gTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGAtca                                                                >  1:1148529/1‑99 (MQ=255)
                                 gTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGa                                                                <  2:4744868/101‑1 (MQ=255)
                                 gTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGa                                                                >  2:3400329/1‑101 (MQ=255)
                                  tCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc                                                               >  1:4203219/1‑101 (MQ=255)
                                  tCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc                                                               >  1:2936278/1‑101 (MQ=255)
                                  tCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc                                                               <  1:4753057/101‑1 (MQ=255)
                                  gccAGCTGGCGCGACAGGATGGTCGAGAATGCGGCGGCGGAGTCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc                                                               <  2:1874007/100‑1 (MQ=255)
                                   ccAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAcc                                                              <  1:3415577/101‑1 (MQ=255)
                                    cAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccg                                                             <  1:533849/101‑1 (MQ=255)
                                      gCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgcc                                                           <  1:3435002/101‑1 (MQ=255)
                                       cTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgccg                                                          <  2:4610343/101‑1 (MQ=255)
                                       cTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGGGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgccg                                                          <  2:914959/101‑1 (MQ=255)
                                        tGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCgg                                                         >  2:624628/1‑101 (MQ=255)
                                        tGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCgg                                                         <  1:3182646/101‑1 (MQ=255)
                                        tGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCgg                                                         >  2:227817/1‑101 (MQ=255)
                                         ggCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCggg                                                        >  2:2054614/1‑101 (MQ=255)
                                            gcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGCGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc                                                     <  1:3992525/101‑1 (MQ=255)
                                            gcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc                                                     <  2:1791872/101‑1 (MQ=255)
                                             cgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcg                                                    >  1:1719047/1‑101 (MQ=255)
                                               acaggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcgcg                                                  >  1:247896/4‑101 (MQ=255)
                                                 aggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCgcggc                                                >  1:4146145/2‑101 (MQ=255)
                                                 aggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCgcggc                                                >  2:1981707/2‑101 (MQ=255)
                                                  ggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCgcggcg                                               <  1:3496899/101‑1 (MQ=255)
                                                  ggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCgcggcg                                               <  1:4468884/101‑1 (MQ=255)
                                                  ggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGGGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATCCCCCCCGGGATGggggcg                                               >  2:1371485/1‑101 (MQ=255)
                                                     tGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACa                                            >  2:3644080/1‑101 (MQ=255)
                                                     tGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACa                                            <  1:2236397/101‑1 (MQ=255)
                                                       gTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAg                                          <  1:444509/101‑1 (MQ=255)
                                                       gTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAg                                          >  2:2774486/1‑101 (MQ=255)
                                                         cGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGc                                        >  1:3006659/1‑101 (MQ=255)
                                                          gagaATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGcc                                       <  2:2203057/101‑1 (MQ=255)
                                                            gaATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGa                                     >  1:1468961/1‑101 (MQ=255)
                                                            gaATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGa                                     <  2:272667/101‑1 (MQ=255)
                                                              aTTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGGGGCGACAAGGCCGATc                                   >  1:3058656/1‑101 (MQ=255)
                                                              aTTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATc                                   >  2:4111144/1‑101 (MQ=255)
                                                                 cgtcgGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGcc                                <  1:4368243/101‑1 (MQ=255)
                                                                  gtcgGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCg                               >  1:1060613/1‑101 (MQ=255)
                                                                    ctgCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTg                             >  1:3820702/3‑101 (MQ=255)
                                                                    cgGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTg                             <  1:3723385/101‑1 (MQ=255)
                                                                        gAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGcca                         >  1:1392064/1‑101 (MQ=255)
                                                                          aGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGCTGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGccacc                       <  1:1186040/101‑1 (MQ=255)
                                                                          aGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGccacc                       <  1:320421/101‑1 (MQ=255)
                                                                           ggCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGccacca                      >  1:845173/1‑101 (MQ=255)
                                                                            gCCTCGTAGCGCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAg                     <  1:4575897/101‑1 (MQ=255)
                                                                            gCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAg                     >  2:3118789/1‑101 (MQ=255)
                                                                            gCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAg                     >  2:4160642/1‑101 (MQ=255)
                                                                               tCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAgcgc                  <  2:1561358/101‑1 (MQ=255)
                                                                                cGTAGCCCGAGGTGATCTTGTGGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGcc                 >  1:3491903/1‑101 (MQ=255)
                                                                                cGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGcc                 >  2:3959462/1‑101 (MQ=255)
                                                                                 gTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCt                <  1:634910/101‑1 (MQ=255)
                                                                                  tAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGcccc               >  2:877637/1‑99 (MQ=255)
                                                                                  tAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTc               <  2:2594358/101‑1 (MQ=255)
                                                                                   aGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCg              >  2:477338/1‑101 (MQ=255)
                                                                                   aGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCg              >  2:3662887/1‑101 (MQ=255)
                                                                                    gCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCgg             <  1:2817082/101‑1 (MQ=255)
                                                                                       cGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGgaga          >  2:3497163/1‑101 (MQ=255)
                                                                                        gAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGAt         <  1:2752173/101‑1 (MQ=255)
                                                                                             gATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCg    >  2:2186961/1‑101 (MQ=255)
                                                                                              aTCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCgg   <  2:4485387/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCGGC  >  NC_014034/3398831‑3399026

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG